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Information on EC 2.4.1.203 - trans-zeatin O-beta-D-glucosyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9ZQ99
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EC Tree
2.4.1.203 trans-zeatin O-beta-D-glucosyltransferaseIUBMB Comments
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
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Word Map
- 2.4.1.203
- seedling
-
planta
-
n-glucosides
-
n-glucosylation
- glycosyltransferases
- anthocyanin
- drought
-
abscisic
-
brassinosteroids
- chlorophyll
- cis-zeatin
-
postgermination
-
cheap
- brassinolide
-
phytohormones
- rhodiola
- salidroside
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cytokinin-related
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overexpressors
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diphosphate-dependent
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br-deficiency
- agriculture
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
ugt76c2, ugt73c5, ugt76c1, ugt85a1, trans-zeatin o-glucosyltransferase, ugt73c1, glucosyltransferase ugt85a1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glucosyltransferase, uridine diphosphoglucose-zeatin O-
-
-
-
-
uridine diphosphoglucose-zeatin O-glucosyltransferase
-
-
-
-
zeatin O-beta-D-glucosyltransferase
-
-
-
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zeatin O-glucosyltransferase
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-
-
-
CK-specific UGT
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-zeatin O-beta-D-glucosyltransferase
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
CAS REGISTRY NUMBER
COMMENTARY
123644-76-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 2-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]ethyl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
UDP + ?
-
-
-
?
UDP-alpha-D-glucose + prop-2-yn-1-yl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
UDP + ?
determination and analysis of the substrate binding structure of UGT73C1, model, overview
-
-
?
UDP-D-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
-
-
?
UDP-glucose + steviol
UDP + ?
determination and analysis of the substrate binding structure of UGT73C1, model, overview
-
-
?
UDP-D-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
-
-
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
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-
-
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
-
-
?
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
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-
-
additional information
?
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-
the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
-
-
-
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
-
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
-
-
?
additional information
?
-
additional information
enzyme UGT85A1 is also active with cis-zeatin
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-
-
additional information
?
-
enzyme UGT85A1 is also active with cis-zeatin
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-
-
additional information
?
-
enzyme UGT85A1 is also active with cis-zeatin
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-
-
additional information
?
-
additional information
enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
-
-
-
additional information
?
-
enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
-
-
-
additional information
?
-
enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
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UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
additional information
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
-
-
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
-
-
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
additional information
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation
-
additional information
additional information
-
steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
UGT85A1 is mainly expressed in the early seedlings, spatial-temporal expression patterns, overview
UGT85A1 is specifically expressed in senescent leaves
additional information
UGT85A1 is specifically expressed in senescent leaves
additional information
additional information
analysis of CK glucosides formation during main developmental transition phases
additional information
analysis of CK glucosides formation during main developmental transition phases
additional information
analysis of CK glucosides formation during main developmental transition phases
additional information
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
additional information
malfunction
the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview
malfunction
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
malfunction
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
malfunction
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
physiological function
glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview
physiological function
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
additional information
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
additional information
-
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
malfunction
additional information
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
malfunction
additional information
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
malfunction
additional information
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
physiological function
additional information
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
additional information
generation of the T-DNA insertion ugt76c1-1 loss-of-function mutant
additional information
generation of the T-DNA insertion ugt76c1-1 loss-of-function mutant
additional information
generation of the T-DNA insertion ugt76c1-1 loss-of-function mutant
additional information
additional information
generation of the T-DNA insertion ugt76c2-1 loss-of-function mutant
additional information
generation of the T-DNA insertion ugt76c2-1 loss-of-function mutant
additional information
generation of the T-DNA insertion ugt76c2-1 loss-of-function mutant
additional information
additional information
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
additional information
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
additional information
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene UGT73C1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene UGT76C1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
gene UGT76C2, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
gene UGT85A1, overexpression of the wild-type enzyme in Arabidopsis thaliana using transfection via Agrobacterium tumefaciens strain GV3101, expression as GFP-tagged enzyme in onion epidermis by particle bombardment
gene UGT85A1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
gene UGT76C1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
gene UGT76C1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
additional information
gene UGT76C2, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
additional information
gene UGT85A1, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
additional information
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme UGT85A1 is sensitive only to trans-zeatin treatment which increases its expression mildly by 1.59times
the CK N-specific UGT76C1 glycosyltransferase shows no response to any of the cytokinins on transcript level
UGT76C2 is activated rapidly in response to exogenously applied cytokinin, extremely elevated trans-zeatin (tZ)-type CKs is observed
enzyme UGT85A1 is sensitive only to trans-zeatin treatment which increases its expression mildly by 1.59times
enzyme UGT85A1 is sensitive only to trans-zeatin treatment which increases its expression mildly by 1.59times
additional information
the CK N-specific UGT76C1 glycosyltransferase shows no response to any of the cytokinins on transcript level
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin, extremely elevated trans-zeatin (tZ)-type CKs is observed
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hou, B.; Lim, E.K.; Higgins, G.S.; Bowles, D.J.
N-Glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana
J. Biol. Chem.
279
47822-47832
2004
Arabidopsis thaliana (Q9SK82), Arabidopsis thaliana (Q9ZQ94), Arabidopsis thaliana (Q9ZQ99), Arabidopsis thaliana
Jin, S.H.; Ma, X.M.; Kojima, M.; Sakakibara, H.; Wang, Y.W.; Hou, B.K.
Overexpression of glucosyltransferase UGT85A1 influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation
Planta
237
991-999
2013
Arabidopsis thaliana (Q9SK82), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (Q9SK82)
Zhou, Y.; Li, W.; You, W.; Di, Z.; Wang, M.; Zhou, H.; Yuan, S.; Wong, N.K.; Xiao, Y.
Discovery of Arabidopsis UGT73C1 as a steviol-catalyzing UDP-glycosyltransferase with chemical probes
Chem. Commun. (Camb.)
54
7179-7182
2018
Arabidopsis thaliana (Q9ZQ99), Arabidopsis thaliana
Smehilova, M.; Dobruskova, J.; Novak, O.; Takac, T.; Galuszka, P.
Cytokinin-specific glycosyltransferases possess different roles in cytokinin homeostasis maintenance
Front. Plant Sci.
7
1264
2016
Arabidopsis thaliana (more), Arabidopsis thaliana (Q9FI99), Arabidopsis thaliana (Q9FIA0)
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