UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview
glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
generation of the T-DNA insertion ugt85a1-1 loss-of-function mutant. UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type, overview
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene UGT85A1, overexpression of the wild-type enzyme in Arabidopsis thaliana using transfection via Agrobacterium tumefaciens strain GV3101, expression as GFP-tagged enzyme in onion epidermis by particle bombardment