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Information on EC 2.4.1.198 - phosphatidylinositol N-acetylglucosaminyltransferase

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IUBMB Comments
Involved in the first step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. In mammalian cells, the enzyme is composed of at least five subunits (PIG-A, PIG-H, PIG-C, GPI1 and PIG-P). PIG-A subunit is the catalytic subunit. In some species, the long-chain acyl groups of the phosphatidyl group are partly replaced by long-chain alkyl or alk-1-enyl groups.
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This record set is specific for:
UNIPROT: Q9QYT7
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
pig-a, gpi-gnt, dscr5, gpi19, gpi-n-acetylglucosaminyltransferase, gpi-glcnac transferase, phosphatidylinositol n-acetylglucosaminyltransferase, gpi15, phosphatidylinositol n-acetylglucosaminyltransferase subunit a, gpi-n-acetylglucosaminyltransferase complex, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetyl-D-glucosaminyltransferase, uridine diphosphoacetylglucosamine alpha1,6-
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UDP-N-acetyl-D-glucosamine:phosphatidylinositol N-acetyl-D-glucosaminyltransferase
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uridine diphosphoacetylglucosamine alpha1,6-acetyl-D-glucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:1-phosphatidyl-1D-myo-inositol 6-(N-acetyl-alpha-D-glucosaminyl)transferase
Involved in the first step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. In mammalian cells, the enzyme is composed of at least five subunits (PIG-A, PIG-H, PIG-C, GPI1 and PIG-P). PIG-A subunit is the catalytic subunit. In some species, the long-chain acyl groups of the phosphatidyl group are partly replaced by long-chain alkyl or alk-1-enyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
144388-35-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + phosphatidylinositol
UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + phosphatidylinositol
UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
show the reaction diagram
involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins
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?
additional information
?
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene mGPI1 clone
GenBank
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PIGQ_MOUSE
581
0
66264
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of FLAG-tagged PIG-C, GST-tagged PIG-A and PIG-H with and without mGPI1 in the mGPI1-deficient F9 cells
mGPI1, DNA sequence determination, expression in embryonal carcinoma F9 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hong, Y.; Oshihi, K.; Watanabe, R.; Endo, Y.; Maeda, Y.; Kinoshita, T.
GPI1 stabilizes an enzyme essential in the first step of glycosylphosphatidylinositol biosynthesis
J. Biol. Chem.
274
18582-18588
1999
Homo sapiens, Mus musculus (Q9QYT7), Mus musculus
Manually annotated by BRENDA team