Information on EC 2.4.1.191 - luteolin-7-O-diglucuronide 4'-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY
2.4.1.191
-
RECOMMENDED NAME
GeneOntology No.
luteolin-7-O-diglucuronide 4'-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucuronate + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] = UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-beta-D-glucuronide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
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luteolin triglucuronide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin-7-O-beta-D-diglucuronide 4'-O-glucuronosyltransferase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-diglucuronide
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-
-
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LDT
-
-
-
-
UDP-glucuronate:luteolin 7-O-diglucuronide-glucuronosyltransferase
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-
-
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UDPglucuronate:luteolin 7-O-diglucuronide-4'-O-glucuronosyl-transferase
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY
115490-50-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-galactoside
show the reaction diagram
-
7.5% of activity with UDPglucuronic acid
-
?
UDP-glucose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
show the reaction diagram
-
-
-
-
-
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
show the reaction diagram
-
-, involved in the sequential biosynthesis of luteolin triglucuronide
-
ir
UDP-xylose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-xyloside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucuronic acid + luteolin-7-O-glucuronide
UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide
show the reaction diagram
-
34% of activity with luteolin 7-O-diglucuronide
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
show the reaction diagram
-
-
-
-
-
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
show the reaction diagram
-
involved in the sequential biosynthesis of luteolin triglucuronide
-
ir
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
not affected by Ca2+ or Mg2+ up to 0.5 and 0.75 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
luteolin
-
0.02 mM, 50% inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.009
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Luteolin 7-O-diglucuronide
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-
0.09
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UDPglucuronic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.14
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
9
-
50% of maximal activity at pH 6 and pH 9
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
mesophyll protoplasts
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
29000
-
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
freezing without glycerol, complete loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ultrogel AcA 44, Sephadex G-25, ultrafiltration, partial purification
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