Information on EC 2.4.1.189 - luteolin 7-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY
2.4.1.189
-
RECOMMENDED NAME
GeneOntology No.
luteolin 7-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucuronate + luteolin = UDP + luteolin 7-O-beta-D-glucuronide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
-
-
luteolin triglucuronide biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin 7-O-glucuronosyltransferase
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SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-
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-
-
-
LGT
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
115490-49-8
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPgalactose + luteolin
UDP + luteolin 7-O-galactoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucuronic acid + apigenin
UDP + apigenin 7-O-glucuronide
show the reaction diagram
-
38% of activity compared to luteolin
-
?
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
first enzyme in the biosynthesis of luteolin triglucuronide
-
ir
UDPxylose + luteolin
UDP + luteolin 7-O-xyloside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
first enzyme in the biosynthesis of luteolin triglucuronide
-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
0.25 mM, 25% stimulation
Mg2+
-
up to 1 mM, 20% stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
luteolin
-
0.14 mM, 60% inhibition
UDP
-
strong inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.008
luteolin
-
-
0.012
UDPglucuronic acid
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.26
UDP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
-
50% of maximal activity at pH 5.6
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.6 - 9
-
50% of maximal activity at pH 5.6 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
no activity in vacuoles
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
34500
-
gel filtration
488822
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without glycerol, complete inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ultrogel AcA 44, DEAE-cellulose, partial purification
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