Information on EC 2.4.1.189 - luteolin 7-O-glucuronosyltransferase

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The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY
2.4.1.189
-
RECOMMENDED NAME
GeneOntology No.
luteolin 7-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucuronate + luteolin = UDP + luteolin 7-O-beta-D-glucuronide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
-
luteolin triglucuronide biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin 7-O-glucuronosyltransferase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glucuronosyltransferase, uridine diphosphoglucuronate-luteolin 7-O-
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-
-
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LGT
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
115490-49-8
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPgalactose + luteolin
UDP + luteolin 7-O-galactoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucuronic acid + apigenin
UDP + apigenin 7-O-glucuronide
show the reaction diagram
-
38% of activity compared to luteolin
-
?
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
-, first enzyme in the biosynthesis of luteolin triglucuronide
-
ir
UDPxylose + luteolin
UDP + luteolin 7-O-xyloside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
first enzyme in the biosynthesis of luteolin triglucuronide
-
ir
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
0.25 mM, 25% stimulation
Mg2+
-
up to 1 mM, 20% stimulation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
luteolin
-
0.14 mM, 60% inhibition
UDP
-
strong inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.008
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luteolin
-
-
0.012
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UDPglucuronic acid
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.26
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UDP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.16
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
50% of maximal activity at pH 5.6
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
9
-
50% of maximal activity at pH 5.6 and pH 9.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
no activity in vacuoles
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34500
-
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
freezing without glycerol, complete inactivation
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STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ultrogel AcA 44, DEAE-cellulose, partial purification
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