Information on EC 2.4.1.189 - luteolin 7-O-glucuronosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Secale cereale

EC NUMBER
COMMENTARY hide
2.4.1.189
-
RECOMMENDED NAME
GeneOntology No.
luteolin 7-O-glucuronosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-glucuronate + luteolin = UDP + luteolin 7-O-beta-D-glucuronide
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
-
-
luteolin triglucuronide biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucuronate:luteolin 7-O-glucuronosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
115490-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDPgalactose + luteolin
UDP + luteolin 7-O-galactoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucose + luteolin
UDP + luteolin 7-O-glucoside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
UDPglucuronic acid + apigenin
UDP + apigenin 7-O-glucuronide
show the reaction diagram
-
38% of activity compared to luteolin
-
?
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
UDPxylose + luteolin
UDP + luteolin 7-O-xyloside
show the reaction diagram
-
10% of activity with UDPglucuronic acid
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDPglucuronic acid + luteolin
UDP + luteolin 7-O-glucuronide
show the reaction diagram
-
first enzyme in the biosynthesis of luteolin triglucuronide
-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
0.25 mM, 25% stimulation
Mg2+
-
up to 1 mM, 20% stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
luteolin
-
0.14 mM, 60% inhibition
UDP
-
strong inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
luteolin
-
-
0.012
UDPglucuronic acid
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26
UDP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
50% of maximal activity at pH 5.6
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 9
-
50% of maximal activity at pH 5.6 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34500
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without glycerol, complete inactivation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50% glycerol, 3 months, 30-50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ultrogel AcA 44, DEAE-cellulose, partial purification
-