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UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
UDP-alpha-D-glucose + apigenin
UDP + apigenin 7-O-beta-D-glucoside
-
peak I-III, 49%, 45% and 95% of activity with naringenin respectively
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
UDP-alpha-D-glucose + luteolin
UDP + luteolin 7-O-beta-D-glucoside
-
peak I, 52% of activity with naringenin
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
UDP-glucose + a flavanone
UDP + a flavanone 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + a flavone
UDP + a flavone 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + diosmetin
UDP + diosmetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + eriodictyol
UDP + eriodictyol 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
UDP-glucose + isosakuranetin
UDP + isosakuranetin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + kaempferol
UDP + kaempferol 3-O-beta-D-glucoside
-
the enzyme can form kaempferol 3,7-O-diglucoside, reaction of EC 2.4.1.91
-
-
?
UDP-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
the enzyme can form kaempferol 3,7-O-diglucoside
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-glucoside
UDP-glucose + quercetin
UDP + quercetin 3-O-beta-D-glucoside
-
reaction of EC 2.4.1.91
-
-
?
UDP-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
-
-
-
?
UDPglucose + 2',4',6',4-tetrahydroxychalcone
UDP + 2',4',6',4-tetrahydroxychalcone 4'-O-glucoside
-
64% of activity with naringenin
-
?
additional information
?
-
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
Citrus mitis
-
-
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
enzyme does not accept other flavanone or flavonol aglycones
trivial name prunin
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
trivial name naringenin
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
trivial name naringenin
trivial name prunin
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
peak I-III
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
90% of activity with naringenin
-
?
UDP-alpha-D-glucose + kaempferol
UDP + kaempferol 7-O-beta-D-glucoside
-
peak I-III, 50%, 46% and 70% of activity with naringenin respectively
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
164% of activity with naringenin
-
?
UDP-alpha-D-glucose + quercetin
UDP + quercetin 7-O-beta-D-glucoside
-
peak I, 42% of activity with naringenin
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
-
-
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
Citrus mitis
-
-
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
-
-
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
-
trivial name hesperetin, 83% of activity with naringenin
-
?
UDP-glucose + hesperetin
UDP + hesperetin 7-O-glucoside
-
peak I, 70% of activity with naringenin
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-glucoside
-
-
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-glucoside
enzyme specificity for UDP-glucose
-
-
?
UDP-glucose + naringenin
UDP + naringenin 7-O-glucoside
-
-
-
-
?
additional information
?
-
-
the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo
-
-
-
additional information
?
-
-
in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected
-
-
-
additional information
?
-
does not use UDP-glucuronic acid, UDP-galactose, ADP-glucose, GDP-glucose, and TDP-glucose with esculetin as the glycosyl acceptor
-
-
?
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UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
UDP-glucose + a flavanone
UDP + a flavanone 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + a flavone
UDP + a flavone 7-O-beta-D-glucoside
-
-
-
-
?
UDP-glucose + a flavonol
UDP + a flavonol 7-O-beta-D-glucoside
-
-
-
-
?
additional information
?
-
-
the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo
-
-
-
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
Citrus mitis
-
-
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
enzyme does not accept other flavanone or flavonol aglycones
trivial name prunin
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
trivial name naringenin
-
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
trivial name naringenin
trivial name prunin
?
UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone
UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside
-
peak I-III
-
?
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McIntosh, C.A.; Latchinian, L.; Mansell, R.L.
Flavanone-specific 7-O-glucosyltransferase activity in Citrus paradisi seedlings: purification and characterization
Arch. Biochem. Biophys.
282
50-57
1990
Citrus x paradisi
brenda
McIntosh, C.A.; Mansell R.L.
Biosynthesis of naringin in Citrus paradisi: UDP-glucosyl-transferase activity in grapefruit seedlings
Phytochemistry
29
1533-1538
1990
Citrus x paradisi
-
brenda
Lewinsohn, E.; Britsch, L.; Mazur, Y.; Gressel, J.
Flavanone glycoside biosynthesis in Citrus. Chalcone synthase, UDP-glucose: flavanone-7-O-glucosyltransferase and -rhamnosyltransferase activities in cell-free extracts
Plant Physiol.
91
1323-1328
1989
Citrus maxima, Citrus mitis
brenda
Durren, R.L.; McIntosh, C.A.
Flavanone-7-O-glucosyltransferase activity from Petunia hybrida
Phytochemistry
52
793-798
1999
Petunia x hybrida
brenda
Noguchi, A.; Sasaki, N.; Nakao, M.; Fukami, H.; Takahashi, S.; Nishino, T.; Nakayama, T.
cDNA cloning of glycosyltransferases from Chinese wolfberry (Lycium barbarum L.) fruits and enzymatic synthesis of a catechin glucoside using a recombinant enzyme (UGT73A10)
J. Mol. Catal. B
55
84-92
2008
Lycium barbarum (B6EWX3)
-
brenda
Liu, X.; Lin, C.; Ma, X.; Tan, Y.; Wang, J.; Zeng, M.
Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis)
Front. Plant Sci.
9
166
2018
Citrus sinensis
brenda