By further transfers of galactosyl residues to the digalactosyldiacylglycerol, trigalactosyldiacylglycerol and tetragalactosyldiacylglycerol are also formed. This enzyme was originally thought to be the major enzyme involved in the production of digalactosyldiacylglycerol in plants as it masked the effect of the true enzyme (EC 220.127.116.11, digalactosyldiacylglycerol synthase) [4,5]. Its activity is localized to chloroplast envelope membranes, but it does not contribute to net galactolipid synthesis in plants .
under normal growth conditions, the in vivo activity of the galactolipid:galactolipid galactosyltransferase is not detectable in the wild type, but becomes apparent in the tgd mutant. The enzyme does not provide the bulk of glactoglycerolipids in the chloroplast, but is speculated to play a role during ozone-induced injury or possibly during senescence of leaves when chloroplast membranes are turned over
alpha-linolenic acid causes drastic increase in activity under limiting concentrations of MgCl2, without affecting its maximum activity at higher MgCl2 concentration, free alpha-linolenic acid alone does not affect the activity; EDTA inhibits stimulation; unsaturated 16- and 18-carbon fatty acids stimulate in presence of MgCl2
dgd2 null mutant, no effects on growth and lipid composition. Dgd1 dgd2 double null mutant, only trace amounts of digalactosyldiacylglycerol, but some monogalactosyldiacylglycerol and unusual oligogalactolipids like tri- and tetragalactosyldiacylglycerol
analysis of dgd1 and dgd2 double mutants show that galactolipid:galactolipid galactosyltransferase is different from the genuine digalactosyldiacylglycerol synthases DGD1 and DGD2, because chloroplasts of a double mutant still are capable of oligogalactolipid biosynthesis., analysis of tgd mutants show that the galactosyl residues are all beta1->6-linked.