Information on EC 2.4.1.182 - lipid-A-disaccharide synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.1.182
-
RECOMMENDED NAME
GeneOntology No.
lipid-A-disaccharide synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
lipid IVA biosynthesis
-
Lipopolysaccharide biosynthesis
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine:2,3-bis-(3-hydroxytetradecanoyl)-beta-D-glucosaminyl-1-phosphate 2,3-bis(3-hydroxytetradecanoyl)-glucosaminyltransferase
Involved with EC 2.3.1.129 (acyl-[acyl-carrier-protein]---UDP-N-acetylglucosamine O-acyltransferase) and EC 2.7.1.130 (tetraacyldisaccharide 4'-kinase) in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of Escherichia coli.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
lcsC
Q5WSK6, Q5WWX7, Q5X5J5
paralogue of the lipid A disaccharide synthase lpxB, lpxB1 forms a transcriptional unit with gnnA encoding a putative UDP-GlcNAc oxidase
lcsC/lpxB1
Q5WSK6, Q5WWX7, Q5X5J5
-
Legionella cytotoxic suppressor C
Q5WSK6, Q5WWX7, Q5X5J5
-
lipid A disaccharide synthase
-
-
-
-
lipid A disaccharide synthase
-
-
lipid A disaccharide synthase
Escherichia coli C41(DE3)
-
-
-
lipid A disaccharide synthase
-
-
LpxB
Arabidopsis thaliana Col-0
F4IF99
-
-
LpxB
Escherichia coli C41(DE3)
-
-
-
synthase, lipid A disaccharide
-
-
-
-
additional information
Q5WSK6, Q5WWX7, Q5X5J5
two paralogues, lpxB1 and lpxB2
CAS REGISTRY NUMBER
COMMENTARY
105843-81-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Arabidopsis thaliana Col-0
-
UniProt
Manually annotated by BRENDA team
gene lpxB gene
-
-
Manually annotated by BRENDA team
MC1061/pSR8, overvproducing strain
-
-
Manually annotated by BRENDA team
strain C41(DE3)
-
-
Manually annotated by BRENDA team
Escherichia coli C41(DE3)
strain C41(DE3)
-
-
Manually annotated by BRENDA team
Escherichia coli MC1061/pSR8
MC1061/pSR8, overvproducing strain
-
-
Manually annotated by BRENDA team
IpxB1, strain Lens; strains Philadelphia-1, Paris, and Lens, preferentially expressed in the exponential and early stationary phases, Acanthamoeba castellanii used as a host cell, construction of a insertion deletion
UniProt
Manually annotated by BRENDA team
IpxB1, strain Paris; strains Philadelphia-1, Paris, and Lens, preferentially expressed in the exponential and early stationary phases, Acanthamoeba castellanii used as a host cell, construction of a insertion deletion
UniProt
Manually annotated by BRENDA team
IpxB2, strain Lens; strains Philadelphia-1, Paris, and Lens, preferentially expressed in the exponential and early stationary phases, Acanthamoeba castellanii used as a host cell, construction of a insertion deletion
UniProt
Manually annotated by BRENDA team
biovars phaseoli CE3 and viciae 8401
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
F4IF99
42fold accumulation of 2,3-diacylglucosamine 1-phosphate in Arabidopsis thaliana atlpxb-1 mutant
malfunction
Arabidopsis thaliana Col-0
-
42fold accumulation of 2,3-diacylglucosamine 1-phosphate in Arabidopsis thaliana atlpxb-1 mutant
-
metabolism
-
catalyzes the fifth step of lipid A biosynthesis
metabolism
Escherichia coli C41(DE3)
-
catalyzes the fifth step of lipid A biosynthesis
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
ir
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
ir
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
product analysis
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
2,3-diacylglucosamine 1-phosphate is lipid X
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
2,3-diacylglucosamine 1-phosphate is lipid X
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
2,3-diacylglucosamine 1-phosphate is lipid X
-
ir
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
2,3-diacylglucosamine 1-phosphate is lipid X
-
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
strong preference for 2,3-diacylated substrates, but the (R)-3-hydroxy substituent is not required
-
ir
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
lipid X isolated from E. coli
-
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
involved with EC 2.3.1.129 and EC 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of E. coli and other gram-negative bacteria
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
involved in lipid A biosynthesis
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
Escherichia coli MC1061/pSR8
-
2,3-diacylglucosamine 1-phosphate is lipid X
-
?
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
Escherichia coli MC1061/pSR8
-
2,3-diacylglucosamine 1-phosphate is lipid X, strong preference for 2,3-diacylated substrates, but the (R)-3-hydroxy substituent is not required
-
ir
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
Escherichia coli MC1061/pSR8
-
involved with EC 2.3.1.129 and EC 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of E. coli and other gram-negative bacteria
-
-
-
UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
show the reaction diagram
Arabidopsis thaliana, Arabidopsis thaliana Col-0
F4IF99
-
-
-
?
UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate
2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP
show the reaction diagram
Haemophilus influenzae, Escherichia coli, Escherichia coli C41(DE3)
-
-
-
-
?
UDP-3-((R)-3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 3-((R)-3-hydroxytetradecanoyl)-N-acetyl-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
Escherichia coli, Escherichia coli MC1061/pSR8
-
0.44% of the activity with UDP-2,3-diacylglucosamine
-
-
?
additional information
?
-
-, Q5WSK6, Q5WWX7, Q5X5J5
involved in the biosynthetic pathway leading to 3-aminoglucosamine analogues of lipid A, responsible for cytotoxic effect on the host Acanthamoeba castellanii
-
-
-
additional information
?
-
Arabidopsis thaliana, Arabidopsis thaliana Col-0
F4IF99
formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
-
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
involved with EC 2.3.1.129 and EC 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of E. coli and other gram-negative bacteria
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
-
involved in lipid A biosynthesis
-
-
-
UDP-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
UDP + 2,3-bis((R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2,3-bis((R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate
show the reaction diagram
Escherichia coli MC1061/pSR8
-
involved with EC 2.3.1.129 and EC 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of E. coli and other gram-negative bacteria
-
-
-
UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-(1->6)-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
show the reaction diagram
Arabidopsis thaliana, Arabidopsis thaliana Col-0
F4IF99
-
-
-
?
additional information
?
-
-, Q5WSK6, Q5WWX7, Q5X5J5
involved in the biosynthetic pathway leading to 3-aminoglucosamine analogues of lipid A, responsible for cytotoxic effect on the host Acanthamoeba castellanii
-
-
-
additional information
?
-
Arabidopsis thaliana, Arabidopsis thaliana Col-0
F4IF99
formation of lipid A disaccharide from UDP-GlcNAc and lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, releasing UDP. Lipid X, i.e. 2,3-diacylglucosamine 1-phosphate, bears two R-3-hydroxymyristoyl chains in Arabidopsis thaliana and is a key precursor of lipid A. No other lipid X molecular species are detected
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no requirement for divalent cations
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
octyl-beta-D-glucoside
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
between 0 and 0.5 mM Triton X-100 increases the apparent specific activity 5fold
additional information
-
no requirement for detergent
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.27
-
2,3-diacylglucosamine 1-phosphate
-
-
0.11
-
UDP-2,3-diacylglucosamine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9
-
-
purified enzyme
15.9
-
-
purified enzyme
45
-
-
enzyme from membrane-free lysate, at 30C
183
-
-
after 4.0fold purification, at 30C
additional information
-
-
specific activity in different recombinant strains
additional information
-
-
coupled assay, first 3 steps of lipid A biosynthesis
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
37
-
-
assay at
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Escherichia coli MC1061/pSR8
-
-
-
Manually annotated by BRENDA team
-
LpxB activity is dependent upon the bulk surface concentration of ist substrates in a mixed micelle assay system, suggesting that catalysis occurs at the membrane interface
Manually annotated by BRENDA team
Escherichia coli C41(DE3)
-
LpxB activity is dependent upon the bulk surface concentration of ist substrates in a mixed micelle assay system, suggesting that catalysis occurs at the membrane interface
-
Manually annotated by BRENDA team
Arabidopsis thaliana Col-0
-
-
-
Manually annotated by BRENDA team
additional information
-
enzyme possibly interacts with membranes due to its extreme hydrophobic reaction product
-
Manually annotated by BRENDA team
additional information
F4IF99
lipid X levels in mitochondria are 3 and 48fold higher than in chloroplasts or whole cell homogenates, respectively. Lipid X is undetectable in the plasma membrane
-
Manually annotated by BRENDA team
additional information
Arabidopsis thaliana Col-0
-
lipid X levels in mitochondria are 3 and 48fold higher than in chloroplasts or whole cell homogenates, respectively. Lipid X is undetectable in the plasma membrane
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
42000
-
-
gel filtration
86000
-
-
gel filtration
360000
-
-
gel filtration
additional information
-
-
nucleotide sequence
additional information
-
-
glycerol-3-phosphate dehydrogenase specifically binds to purified His-tagged enzyme
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 42000, SDS-PAGE
?
-
x * 42339, DNA sequence determination
?
Escherichia coli MC1061/pSR8
-
x * 42339, DNA sequence determination
-
dimer
-
2 * 42000, SDS-PAGE
dimer
Escherichia coli MC1061/pSR8
-
2 * 42000, SDS-PAGE
-
homooctamer
-
8 * 43000, SDS-PAGE
homooctamer
Escherichia coli C41(DE3)
-
8 * 43000, SDS-PAGE
-
monomer
-
1 * 42000, SDS-PAGE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
30 min, inactivation
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, frozen in liquid N2, stable for several years
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography and Superdex 200 gel filtration
-
purification as recombinant His-tagged protein from overproducing strain in presence of Triton X-100, copurification of glycerol-3-phosphate dehydrogenase specifically bound to the enzyme
-
purification of lpxB-lacZ gene product
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli strain XL1-Blue
-
expression from plasmid, DNA sequence analysis, lpxB gene is cotranscribed with the lpxA gene located in the same operon
-
overexpression from plasmid as His-tagged protein
-
overexpression in Escherichia coli from plasmid, alone and together with gene lpxA, UDP-GlcNAc acyltransferase
-
expressed in Escherichia coli MN7 (temperature sensitive lpxB mutant), expression of protein complements growth at 30 and 42C; expressed in Escherichia coli MN7 (temperature sensitive lpxB mutant), expression of protein complements growth at 30 and 42C; expressed in Escherichia coli MN7 (temperature sensitive lpxB mutant), expression of protein complements growth at 30 and 42C
-, Q5WSK6, Q5WWX7, Q5X5J5
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D89A
-
mutant shows no residual catalytic activity
R201A
-
mutant shows no residual catalytic activity
D89A
Escherichia coli C41(DE3)
-
mutant shows no residual catalytic activity
-
R201A
Escherichia coli C41(DE3)
-
mutant shows no residual catalytic activity
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
synthesis of a variety of artificial fluorinated lipid A precursor analogues on a preparative scale for investigation of structure-function relationships of lipid A derivatives