Information on EC 2.4.1.166 - raffinose-raffinose alpha-galactosyltransferase

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The expected taxonomic range for this enzyme is: Cerastium arvense

EC NUMBER
COMMENTARY
2.4.1.166
-
RECOMMENDED NAME
GeneOntology No.
raffinose-raffinose alpha-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2 raffinose = 1F-alpha-D-galactosylraffinose + sucrose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
lychnose and isolychnose biosynthesis
-
stellariose and mediose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
raffinose:raffinose alpha-D-galactosyltransferase
The 3F position of raffinose can also act as galactosyl acceptor; the enzyme is involved in the accumulation of the tetrasaccharides lychnose and isolychnose in the leaves of Cerastium arvense and other plants of the family Caryophyllaceae during late autumn.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
galactosyltransferase, raffinose (raffinose donor)
-
-
-
-
raffinose:raffinose alpha-galactosyltransferase
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
93389-38-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
enzyme can also be isolated from other species of the Caryophyllaceae
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
raffinose + raffinose
lychnose + sucrose
show the reaction diagram
-
-
i.e. 1F-alpha-D-galactosylraffinose
?
raffinose + raffinose
isolychnose + sucrose
show the reaction diagram
-
-
-, isolychnose is 3F-alpha-galactosylraffinose, enzyme can also be found in Cerastium arvense leaves, probably another raffinose:raffinose alpha-D-galactosyltransferase
?
additional information
?
-
-
involved in the accumulation of the tetrasaccharides lychnose and isolychnose in the leaves of Cerastium arvense and other Caryophyllaceae during late autumn
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
raffinose + raffinose
lychnose + sucrose
show the reaction diagram
-
-
i.e. 1F-alpha-D-galactosylraffinose
?
raffinose + raffinose
isolychnose + sucrose
show the reaction diagram
-
-
-
?
additional information
?
-
-
involved in the accumulation of the tetrasaccharides lychnose and isolychnose in the leaves of Cerastium arvense and other Caryophyllaceae during late autumn
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00025
-
-
lychnose
0.000316
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-
isolychnose
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
38
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE