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Information on EC 2.4.1.165 - N-acetylneuraminylgalactosylglucosylceramide beta-1,4-N-acetylgalactosaminyltransferase and Organism(s) Homo sapiens and UniProt Accession Q8NHY0
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2.4.1.165 N-acetylneuraminylgalactosylglucosylceramide beta-1,4-N-acetylgalactosaminyltransferaseIUBMB Comments
Requires Mn2+. Only substances containing sialic acid residues can act as acceptors; bovine fetuin is the best acceptor tested.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
galgt2, beta4galnact-ii, ct galnac transferase, beta4galnac-t, udp-galnac:beta1,4-n-acetylgalactosaminyltransferase, beta-1,4-n-acetylgalactosyltransferase ii, cytotoxic t cell galnac transferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-acetylneuraminyl(alpha2-->3)galactosyl(beta1-->4)glucosyl beta1-->4-
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-
-
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CT GalNAc transferase
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-
-
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Sda-beta-1,4-N-acetylgalactosaminyltransferase
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-
-
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Sda-beta-GalNAc-transferase
-
-
-
-
additional information
the short- and long-form human Sd(alpha) beta4GalNAcT-II transcripts are composed of different exon 1, exon 1S, and exon 1L
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-galactosamine:N-acetylneuraminyl-(2->3)-alpha-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 4-beta-N-acetylgalactosaminyltransferase
Requires Mn2+. Only substances containing sialic acid residues can act as acceptors; bovine fetuin is the best acceptor tested.
CAS REGISTRY NUMBER
COMMENTARY
109136-50-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + alpha(2-3)-sialylated glycoprotein
UDP + GalNAc-beta(1-4)(Neu5Ac(2-3))Gal-beta-1-protein
-
-
-
?
UDP-N-acetyl-D-galactosamine + Neu5Ac-alpha(2-3)Gal-beta(1-3)GlcNAc-beta-1-O-benzyl
UDP + ?
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
responsible for the final step in the formation of the Sda/Cad antigenic epitopes, Sd(alpha) beta4GalNAcT-II mRNA transcript is drastically reduced in oncogenetic processes in gastrointestinal tissues
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP-N-acetyl-D-galactosamine + alpha1-acid glycoprotein
UDP + N-acetylgalactosaminyl-alpha1-acid glycoprotein
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + asialofetuin
UDP + N-acetylgalactosaminylasialofetuin
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + di-sialyl-lacto-N-tetraose
UDP + N-acetylgalactosaminyl-di-sialyl-lacto-N-tetraose
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + fetuin
UDP + N-acetylgalactosaminylfetuin
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-
-
-
?
UDP-N-acetyl-D-galactosamine + glycophorin
UDP + N-acetylgalactosaminyl glycophorin
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good acceptor
-
-
?
UDP-N-acetyl-D-galactosamine + glycophorin A
UDP + N-acetylgalactosaminyl glycophorin A
-
weak activity
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin
UDP + N-acetylgalactosaminyl human chorionic gonadotropin
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNAc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
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enzymic activity correlates with the degree of enterocytic differentiation
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-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
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responsible for addition of the immunodominant sugar of the Sda-isto-blood-group determinant
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
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strictly requires the presence of sialic acid in alpha-2,3-linkage to subterminal galactose
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
i.e. 3'-sialyllactose
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNAc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNAc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
-
i.e. 3'-sialyllactosamine
-
-
?
additional information
?
-
involved in formation of blood group Sda/CAD antigen
-
-
?
additional information
?
-
analysis of acceptor substrate specificity, no substrate: ganglioside GM3, 3-sulfolactose, 3-O-methylcarboxyllactose
-
-
?
additional information
?
-
-
the enzyme strictly requires the presence in acceptors of NeuAc in alpha2,3-linkage to subterminal galactose
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-galactosamine + NeuAcalpha(2-3)Galbeta(1-4)Glc
UDP + GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc
responsible for the final step in the formation of the Sda/Cad antigenic epitopes, Sd(alpha) beta4GalNAcT-II mRNA transcript is drastically reduced in oncogenetic processes in gastrointestinal tissues
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
additional information
?
-
involved in formation of blood group Sda/CAD antigen
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
enzymic activity correlates with the degree of enterocytic differentiation
-
-
?
UDP-N-acetyl-D-galactosamine + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-[alpha-N-acetylneuraminyl-(2->3)]-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
responsible for addition of the immunodominant sugar of the Sda-isto-blood-group determinant
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
enzymic activity correlates with the degree of enterocytic differentiation
-
colon mucosa or colon carcinoma cell line CaCo-2, not in cell lines SW-948, SW-948FL, SW-480, SW-48, SW-1417, COLO-205, LOVO or HT-29
additional information
detected in various amounts in all cell types studied
additional information
other cell lines than differentiated Caco2 cells or normal colonic mucosa show low level of enzyme
additional information
-
other cell lines than differentiated Caco2 cells or normal colonic mucosa show low level of enzyme
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B4GN2_HUMAN
566
0
63258
Swiss-Prot
Mitochondrion (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
protein has a typical type II glycosyltransferase organization and a 67 amino acid cytoplasmic tail
additional information
-
protein has a typical type II glycosyltransferase organization and a 67 amino acid cytoplasmic tail
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takeya, A.; Hosomi, O.; Kogure, T.
Identification and characterization of UDP-GalNAc: NeuAcalpha2-3Galbeta1-4Glc(NAc) beta 1-4(GalNAc to Gal)N-acetylgalactosaminyltransferase in human blood plasma
J. Biochem.
101
251-259
1987
Homo sapiens
Malagolini, N.; Dall'Olio, F.; Serafini-Cessi, F.
UDP-GalNAc: NeuAc alpha 2,3Gal beta-R (GalNAc to Gal) beta 1,4-N-acetylgalactosaminyltransferase responsible for the Sda specificity in human colon carcinoma CaCo-2 cell line
Biochem. Biophys. Res. Commun.
180
681-686
1991
Homo sapiens
Morton, J.A.; Pickles, M.M.; Vanhegan, R.I.
The Sda antigen in the human kidney and colon
Immunol. Invest.
17
217-224
1988
Homo sapiens
Malagolini, N.; Dall'Olio, F.; Guerrini, S.; Serafini-Cessi, F.
Identification and characterization of the Sda beta1,4-N-acetylgalactosaminyltransferase from pig large intestine
Glycoconj. J.
11
89-95
1994
Homo sapiens, Rattus norvegicus, Sus scrofa
Serafini-Cessi, F.; Malagolini, N.; Guerrini, S.; Turrini, I.
A soluble form of Sda-beta1,4-N-acetylgalactosaminyltransferase is released by differentiated human colon carcinoma CaCo-2 cells
Glycoconj. J.
12
773-779
1995
Homo sapiens
Montiel, M.D.; Krzewinski-Recchi, M.A.; Delannoy, P.; Harduin-Lepers, A.
Molecular cloning, gene organization and expression of the human UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1,4-N-acetylgalactosaminyltransferase responsible for the biosynthesis of the blood group Sda/Cad antigen: evidence for an unusual extended cytoplasmic domain
Biochem. J.
373
369-379
2003
Homo sapiens (Q8NHY0)
Lo Presti, L.; Cabuy, E.; Chiricolo, M.; Dall'Olio, F.
Molecular cloning of the human beta1,4N-acetylgalactosaminyltransferase responsible for the biosynthesis of the Sda histo-blood group antigen: The sequence predicts a very long cytoplasmic domain
J. Biochem.
134
675-682
2003
Homo sapiens (Q8NHY0), Homo sapiens
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