Information on EC 2.4.1.150 - N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.150
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RECOMMENDED NAME
GeneOntology No.
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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terminal O-glycans residues modification
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminide 6-beta-N-acetyl-D-glucosaminyltransferase
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-40-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Xenopus (Silurana) tropicalis
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
C2GnT2 deficiency, impair of mucosal barrier, increase of susceptibility to colitis, reduced immunoglobulin abundance, loss of all core 4 O-glycan biosynthetic activity
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
?
show the reaction diagram
-
reaction rate is 38% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)(Fucalpha(1-3))GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
very poor substrate
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
i.e. lacto-N-neotetraose, IGnT B forms branch in the internal Gal residue
no transfer to the terminal Gal residue
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + (GlcNAc)1-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
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cIGnT
also as product: (GlcNAc)2-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, cIGnT6 generates in a partial reaction nona- and decasaccharide products, which represent mixtures of isomers carrying one or two GlcNAc-branches on the linear octasaccharide acceptor
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-3)GalNAcalpha1-R
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-3)GalNAcalpha1-R
show the reaction diagram
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dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
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dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)Glc(NAc)beta1-R
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc(NAc)beta1-R
show the reaction diagram
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dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
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very poor acceptor, reaction rate is 2% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
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?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Gal
?
show the reaction diagram
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poor acceptor, reaction rate is 6% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
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?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
UDP-N-acetyl-D-glucosamine + lactose
UDP + GlcNAcbeta(1-6)Galbeta(1-4)Glc
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-6)Galbeta(1-4)GlcNAc
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + oligo-N-acetyllactosaminoglycan
?
show the reaction diagram
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involved in midchain branching of oligo-N-acetyllactosaminoglycans by transferring GlcNAc in beta1,6-linkage to internal galactose residues
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UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
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might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
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UDP-N-acetyl-D-glucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
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poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure
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?
UDP-N-acetyl-D-glucosamine + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
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i.e. pyridylaminated lacto-N-neotetraose, cIGnT6
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?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + oligo-N-acetyllactosaminoglycan
?
show the reaction diagram
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involved in midchain branching of oligo-N-acetyllactosaminoglycans by transferring GlcNAc in beta1,6-linkage to internal galactose residues
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UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
-
might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
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additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
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CdCl2, slightly stimulates
Mg2+
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MgCl2, slightly stimulates
additional information
-
no requirement for any divalent metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AMP
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10% inhibition
CuCl2
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GDP
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19% inhibition
NiCl2
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UDP
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most potent inhibitor of donor substrate analogues, complete inhibition
UDP-galactose
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42% inhibition
UDP-glucose
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51% inhibition
UDP-glucuronic acid
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36% inhibition
UDP-hexanolamine
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66% inhibition
UMP
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41% inhibition
UTP
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most potent inhibitor of donor substrate analogues, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.55
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.52
NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.96
pyridylaminated lacto-N-neotetraose
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2.59
UDP-N-acetyl-D-glucosamine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.058
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additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 7.8
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broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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IGnT is highly expressed in adult cerebellum and the frontal lobe of adult brain, also expression in fetal brain
Manually annotated by BRENDA team
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IGnT is highly expressed in adult cerebellum
Manually annotated by BRENDA team
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IGnT is moderately expressed in adult colon
Manually annotated by BRENDA team
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native and knockout mutants, mutant cells exhibited a reduced capability in embryoglycan synthesis and adhere weakly to laminin-coated plates
Manually annotated by BRENDA team
D-3 embryonic stem cells moderately express IGnT A and B; D-3 embryonic stem cells moderately express IGnT A and B
Manually annotated by BRENDA team
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F9 embryonal carcinoma cells moderately express IGnT A and B
Manually annotated by BRENDA team
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IGnT is moderately expressed in adult heart
Manually annotated by BRENDA team
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C2GnT, promyelocytic leukaemia HL-60 cells
Manually annotated by BRENDA team
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IGnT expression in fetal lung
Manually annotated by BRENDA team
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C2GnT is highly expressed in
Manually annotated by BRENDA team
P-19 embryonal carcinoma cells moderately express IGnT A and B; P-19 embryonal carcinoma cells moderately express IGnT A and B
Manually annotated by BRENDA team
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IGnT is highly expressed in adult prostata
Manually annotated by BRENDA team
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C2GnT is highly expressed in activated T lymphocytes
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
enzyme is concentrated in the microsome fraction
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Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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cristallographic analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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recombinant enzyme, expressed in SF9 insect cells as fusion protein with glutathione S-transferase has 5 potential N-glycosylation sites
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin, 2 mg/ml, preserves enzyme activity at 37°C, especially the highly purified enzyme fraction
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, recombinant enzyme, a few days, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
210000fold, cIGnT6
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cIGnT, purified from PA-1 cells
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purification of recombinant enzyme, expressed in SF9 insect cells as protein fused to glutathione S-transferase
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; 2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; IGnT A is cloned; IGnT A is cloned; IGnT B is cloned from mouse liver cDNA, nucleotide and amino acid sequence, exon organization of the IGnT gene, expression of IGnT A and B in COS-7 and CHO cells; IGnT B is cloned from mouse liver cDNA, nucleotide and amino acid sequence, exon organization of the IGnT gene, expression of IGnT A and B in COS-7 and CHO cells
2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; expression of each isoform is controlled by a different promoter, characterization of the promoters: GT boxes play crucial roles in transcriptional regulation of the genes; IGnT A is cloned
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C2GnT and IGnT cDNAs are cloned and sequenced, genes are both localized on chromosome 9, band q2l, genomic structures
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cDNA encoding IGnT is cloned from PA-1 cells, 400-amino acids protein
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IGnT6 is cloned, sequenced and cDNA encoding cIGnT6 in PA-1 cells is expressed in SF9 insect cells as protein fused to glutathione S-transferase
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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mutation experiments at the GT boxes of the promoters of IGnT A and B genes
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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cIGnT6 transfers multiple GlcNAc branches to long linear polylactosamines, a prerequisite for improving enzyme-assisted in vitro synthesis of a type of multivalent sialyl Lewis x glycans that are high affinity inhibitors of lymphocyte L-selectin, enzyme allows general polylactosamine synthesis