Information on EC 2.4.1.150 - N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.4.1.150
-
RECOMMENDED NAME
GeneOntology No.
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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-
-
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UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-R
show the reaction diagram
mechanism, model for regulation by putrescine
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
terminal O-glycans residues modification
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminide 6-beta-N-acetyl-D-glucosaminyltransferase
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoprotein.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->6-
-
-
-
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beta1-6GnT
-
-
-
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Galbeta1-->4GlcNAc-R beta1-->6 N-acetylglucosaminyltransferase
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-
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Gcnt2 gene product
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Gcnt2 gene product
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gene contains one variable exon and two constant exons
I beta1,6-N-acetylglucosaminyltransferase
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I N-acetylglucosaminyltransferase
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-
-
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IGnT
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-
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N-acetylglucosaminyltransferase
-
-
-
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UDP-GlcNAc:Gal-R, beta-D-6-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->6-acetylglucosaminyltransferase
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-
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additional information
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C2GnT forms the core 2 O-glycan branch, IGnT forms the I antigen, both are members of a beta-1,6-N-acetylglucosaminyltransferase gene family
CAS REGISTRY NUMBER
COMMENTARY
85638-40-0
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
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Manually annotated by BRENDA team
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
IGnT A, 2 isoenzymes: IGnT A and B
UniProt
Manually annotated by BRENDA team
IGnT B, 2 isoenzymes: IGnT A and B
UniProt
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
-
-
Manually annotated by BRENDA team
gene contains three highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
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Manually annotated by BRENDA team
Sprague Dawley rats
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Manually annotated by BRENDA team
gene contains two highly similar variable exons and two constant exons, each variable exon is preceded by a distinct promoter and is separately spliced to a set of two constant exons to generate functional Gcnt2 mRNA
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
C2GnT2 deficiency, impair of mucosal barrier, increase of susceptibility to colitis, reduced immunoglobulin abundance, loss of all core 4 O-glycan biosynthetic activity
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
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acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins
-
?
UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein
UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
show the reaction diagram
-
acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins, GlcNAc residues are introduced to position C-6 of the terminal galactose of the glycoprotein, relative high activity towards asialo-alpha1-acid glycoprotein in Novikoff ascites tumor cells
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
show the reaction diagram
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acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins, GlcNAc residues are introduced to position C-6 of the terminal galactose of the glycoprotein
-
?
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT A: at 256% of the rate with lacto-N-neotetraose
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-
?
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT B: at 148% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
?
show the reaction diagram
-
reaction rate is 38% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
poor substrate
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT B: at 6% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT A: at 4% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
poor substrate
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT B: at 10% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT A: at 6% of the rate with lacto-N-neotetraose
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-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)(Fucalpha(1-3))GlcNAcbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
very poor substrate
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?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
i.e. lacto-N-neotetraose, IGnT B forms branch in the internal Gal residue
no transfer to the terminal Gal residue
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
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cIGnT6
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
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cIGnT
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
IGnT B: at 181% of the rate with lacto-N-neotetraose
IGnT B: major product, minor product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc, IGnT B forms beta-1,6 branch in both of the internal galactosyl residues, prolonged incubation results in di-branched oligosaccharide
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
IGnT A: at 170% of the rate with lacto-N-neotetraose
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
i.e. para-lacto-N-neohexaose
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
show the reaction diagram
i.e. para-lacto-N-neohexaose
IGnT B: major product, minor product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc, IGnT B forms beta-1,6 branch in both of the internal galactosyl residues, prolonged incubation results in di-branched oligosaccharide
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
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cIGnT6
cIGnT6, 60% of the heptasaccharide product, 40% of the heptasaccharide product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc, 95% heptasaccharide and 5% di-branched octasaccharide product
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
IGnT B: at 164% of the rate with lacto-N-neotetraose
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
IGnT A: at 194% of the rate with lacto-N-neotetraose
-
?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + (GlcNAc)1-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
show the reaction diagram
-
cIGnT
also as product: (GlcNAc)2-Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, cIGnT6 generates in a partial reaction nona- and decasaccharide products, which represent mixtures of isomers carrying one or two GlcNAc-branches on the linear octasaccharide acceptor
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-3)GalNAcalpha1-R
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-3)GalNAcalpha1-R
show the reaction diagram
-
dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
-
dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)Glc(NAc)beta1-R
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc(NAc)beta1-R
show the reaction diagram
-
dIGnT
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
-
very poor acceptor, reaction rate is 2% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Gal
?
show the reaction diagram
-
poor acceptor, reaction rate is 6% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
-
reaction rate is 41% of that with GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
IGnT A: at 22% of the rate with lacto-N-neotetraose
only product
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
IGnT B: at 41% of the rate with lacto-N-neotetraose
only product
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
-
GlcNAc residue at the reducing end side of the branching galactose plays a role in the reaction
-
?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAc
show the reaction diagram
-
cIGnT6
-
?
UDP-N-acetyl-D-glucosamine + lactose
UDP + GlcNAcbeta(1-6)Galbeta(1-4)Glc
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyllactosamine
UDP + GlcNAcbeta(1-6)Galbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT B: at 123% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT A: at 35% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT A: at 256% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
?
show the reaction diagram
IGnT B: at 148% of the rate with lacto-N-neotetraose
-
-
?
UDP-N-acetyl-D-glucosamine + oligo-N-acetyllactosaminoglycan
?
show the reaction diagram
-
involved in midchain branching of oligo-N-acetyllactosaminoglycans by transferring GlcNAc in beta1,6-linkage to internal galactose residues
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
cIGnT6
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
forms branches in poly-N-acetyllactosamines
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
forms branches in poly-N-acetyllactosamines
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
forms branches in poly-N-acetyllactosamines
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
forms branches in poly-N-acetyllactosamines
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
transfer of GlcNAc to beta1,4-linked Gal residue in a linear poly-N-acetyllactosamine with the approximate structure Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc(NAc)-R, forming Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc(NAc)-R
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
cIGnT6 transfers one or multiple GlcNAc branches to midchain galactoses of long linear polylactosamines
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
IGnT
-
-
?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
-
might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
-
-
-
UDP-N-acetyl-D-glucosamine + porcine submaxillary asialo-afuco-mucin
UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
show the reaction diagram
-
poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure
-
?
UDP-N-acetyl-D-glucosamine + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
UDP + pyridylaminated Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc
show the reaction diagram
-
i.e. pyridylaminated lacto-N-neotetraose, cIGnT6
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
2 isoforms IGnT A and IGnT B, C-terminal 1/4 of IGnT B is identical to that of IGnT A, the rest of the sequences shows 63% identity
-
-
-
additional information
?
-
2 isoforms IGnT A and IGnT B, C-terminal 1/4 of IGnT B is identical to that of IGnT A, the rest of the sequences shows 63% identity
-
-
-
additional information
?
-
-
2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues
-
-
-
additional information
?
-
-
2 types of branching enzyme activities: cIGnT6 generates beta-1,6-N-acetylglucosaminyl branches at the central galactose residue, and dIGnT6 acts on peridistal galactose residues
-
-
-
additional information
?
-
generally, oligosaccharides with the Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc(Nac) sequence serve as good substrates, addition of Galalpha(1-3) or sialic acid alpha(2-6) to the non-reducing end gelactose enhances the acceptor activity, while sialic acid alpha2-3 linkage shows a repressive effect, no substrate: N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
-
-
additional information
?
-
-
no substrate of cIGnT6: GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc, Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)(Fucalpha(1-3))GlcNAc, no transfer of GlcNAc to substrates with alpha1-3-fucosyl residues and to midchain galactoses that belongs to Lewis x determinants
-
-
-
additional information
?
-
-
absolute requirement of at least a complete Galbeta(1-4)GlcNAc residue bound to position 3 of the acceptor Gal residues, i.e. it is capable of acting only on the Gal residues of internal Galbeta(1-4)GlcNAc units, no substrates: pyridylaminated GlcNAcbeta(1-3)Galbeta(1-4)Glc and pyridylaminated Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc, 4 major groups according to their acceptor specificities: blood group I structure: IGnT6, core 2 in O-glycans: C2GnT6, core 4 in O-glycans: C4GnT6, 2,6-branched N-linked core: GnT V
-
-
-
additional information
?
-
-
initiates formation of side chains, key enzyme in biosynthesis of I antigen of erythrocytes, N-acetyllactosamine is a more physiological acceptor than lactose
-
-
-
additional information
?
-
-
expression of I-antigen is entirely dependent on IGnT, expression of IGnT is developmentally regulated
-
-
-
additional information
?
-
-
C2GnT forms the core 2 O-glycan branch, which is critical for oligosaccharide-mediated cell-cell interaction, IGnT forms the I antigen, both are members of a beta-1,6-N-acetylglucosaminyltransferase gene family
-
-
-
additional information
?
-
-
a major enzyme involved in the branching of poly-N-acetyllactosamine chains in embryoglycan, branching of poly-N-acetyllactosamine chains is performed by beta1,6-N-acetylglucosaminylation of the galactosyl residue
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + oligo-N-acetyllactosaminoglycan
?
show the reaction diagram
-
involved in midchain branching of oligo-N-acetyllactosaminoglycans by transferring GlcNAc in beta1,6-linkage to internal galactose residues
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
responsible for the conversion of linear to branched polylactosamines, cIGnT6 actions at central rather than peridistal galactose residues of linear polylactosamines in the biosynthesis of blood group I antigens
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
A2IQH4, A2IQH5
forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine
?
show the reaction diagram
-
responsible for the formation of the beta1-6-branched poly-N-acetyllactosamine structure, involved in generating branches to central positions of preformed as well as growing polylactosamine chains but not in synthesizing the distal branches to growing chains
-
-
-
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan
?
show the reaction diagram
-
might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans
-
-
-
additional information
?
-
-
initiates formation of side chains, key enzyme in biosynthesis of I antigen of erythrocytes, N-acetyllactosamine is a more physiological acceptor than lactose
-
-
-
additional information
?
-
-
expression of I-antigen is entirely dependent on IGnT, expression of IGnT is developmentally regulated
-
-
-
additional information
?
-
-
C2GnT forms the core 2 O-glycan branch, which is critical for oligosaccharide-mediated cell-cell interaction, IGnT forms the I antigen, both are members of a beta-1,6-N-acetylglucosaminyltransferase gene family
-
-
-
additional information
?
-
-
a major enzyme involved in the branching of poly-N-acetyllactosamine chains in embryoglycan, branching of poly-N-acetyllactosamine chains is performed by beta1,6-N-acetylglucosaminylation of the galactosyl residue
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cd2+
-
CdCl2, slightly stimulates
Mg2+
-
MgCl2, slightly stimulates
Mn2+
-
MnCl2, 10-25 mM for optimal activity required
Mn2+
-
requirement
additional information
-
no requirement for any divalent metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
AMP
-
10% inhibition
CuCl2
-
-
GDP
-
19% inhibition
NiCl2
-
-
UDP
-
most potent inhibitor of donor substrate analogues, complete inhibition
UDP-galactose
-
42% inhibition
UDP-glucose
-
51% inhibition
UDP-glucuronic acid
-
36% inhibition
UDP-hexanolamine
-
66% inhibition
UMP
-
41% inhibition
UTP
-
most potent inhibitor of donor substrate analogues, complete inhibition
ZnCl2
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.44
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.55
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.52
NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc
IGnT B
0.96
pyridylaminated lacto-N-neotetraose
-
-
2.59
UDP-N-acetyl-D-glucosamine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.058
-
-
additional information
when expressed as proteins IGnT B shows higher specific activity than IGnT A; when expressed as proteins IGnT B shows higher specific activity than IGnT A
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.8 - 7.8
-
broad
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
assay at
37
-
assay at
37
assay at; assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
IGnT is highly expressed in adult cerebellum and the frontal lobe of adult brain, also expression in fetal brain
Manually annotated by BRENDA team
-
IGnT is highly expressed in adult cerebellum
Manually annotated by BRENDA team
-
IGnT is moderately expressed in adult colon
Manually annotated by BRENDA team
-
native and knockout mutants, mutant cells exhibited a reduced capability in embryoglycan synthesis and adhere weakly to laminin-coated plates
Manually annotated by BRENDA team
D-3 embryonic stem cells moderately express IGnT A and B; D-3 embryonic stem cells moderately express IGnT A and B
Manually annotated by BRENDA team
-
F9 embryonal carcinoma cells moderately express IGnT A and B
Manually annotated by BRENDA team
-
IGnT is moderately expressed in adult heart
Manually annotated by BRENDA team
-
C2GnT, promyelocytic leukaemia HL-60 cells
Manually annotated by BRENDA team
-
strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
strong expression of IGnT A and B in adults; strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
-
strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
strong expression of IGnT A and B in adults; strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
-
IGnT expression in fetal kidney
Manually annotated by BRENDA team
-
strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
strong expression of IGnT A and B in adults; strong expression of IGnT A and B in adults
Manually annotated by BRENDA team
-
IGnT expression in fetal lung
Manually annotated by BRENDA team
-
moderate expression of IGnT A and B
Manually annotated by BRENDA team
moderate expression of IGnT A and B; moderate expression of IGnT A and B
Manually annotated by BRENDA team
P-19 embryonal carcinoma cells moderately express IGnT A and B; P-19 embryonal carcinoma cells moderately express IGnT A and B
Manually annotated by BRENDA team
-
IGnT, teratocarcinoma PA-1 cells
Manually annotated by BRENDA team
-
cIGnT6, embryonal carcinoma cell line
Manually annotated by BRENDA team
-
tetracarcinoma cells, IGnT mainly acts as cIGnT, but can also act as dIGnT with 6-30% of the activity of cIGnT
Manually annotated by BRENDA team
-
IGnT is highly expressed in adult prostata
Manually annotated by BRENDA team
-
from freshly drawn human blood of healthy donors
Manually annotated by BRENDA team
-
highest enzyme activity per tissue protein
Manually annotated by BRENDA team
-
IGnT is moderately expressed in adult small intestine
Manually annotated by BRENDA team
-
cIGnT is the dominant form
Manually annotated by BRENDA team
-
moderate expression of IGnT A and B
Manually annotated by BRENDA team
moderate expression of IGnT A and B; moderate expression of IGnT A and B
Manually annotated by BRENDA team
-
C2GnT is highly expressed in activated T lymphocytes
Manually annotated by BRENDA team
-
C2GnT is highly expressed in
Manually annotated by BRENDA team
additional information
-
no expression of IGnT A and B in N2a cells
Manually annotated by BRENDA team
additional information
-
high expression of C2GnT in granulocyte-monocyte cell lineage
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
type II transmembrane topology; type II transmembrane topology
Manually annotated by BRENDA team
-
type II transmembrane topology
Manually annotated by BRENDA team
-
enzyme is concentrated in the microsome fraction
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 67000-74000, glycosylated protein fused to glutathione S-transferase, size heterogenicity is at least partially due to differences in N-glycosylation, SDS-PAGE
?
-
x * 76000, SDS-PAGE under nonreducing conditions
monomer
-
cristallographic analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
recombinant enzyme, expressed in SF9 insect cells as fusion protein with glutathione S-transferase has 5 potential N-glycosylation sites
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bovine serum albumin, 2 mg/ml, preserves enzyme activity at 37°C, especially the highly purified enzyme fraction
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, recombinant enzyme, a few days, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cIGnT, purified from PA-1 cells
-
purification of recombinant enzyme, expressed in SF9 insect cells as protein fused to glutathione S-transferase
-
210000fold, cIGnT6
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C2GnT and IGnT cDNAs are cloned and sequenced, genes are both localized on chromosome 9, band q2l, genomic structures
-
cDNA encoding IGnT is cloned from PA-1 cells, 400-amino acids protein
-
IGnT6 is cloned, sequenced and cDNA encoding cIGnT6 in PA-1 cells is expressed in SF9 insect cells as protein fused to glutathione S-transferase
-
2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; 2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; IGnT A is cloned; IGnT A is cloned; IGnT B is cloned from mouse liver cDNA, nucleotide and amino acid sequence, exon organization of the IGnT gene, expression of IGnT A and B in COS-7 and CHO cells; IGnT B is cloned from mouse liver cDNA, nucleotide and amino acid sequence, exon organization of the IGnT gene, expression of IGnT A and B in COS-7 and CHO cells
2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A; expression of each isoform is controlled by a different promoter, characterization of the promoters: GT boxes play crucial roles in transcriptional regulation of the genes; IGnT A is cloned
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
mutation experiments at the GT boxes of the promoters of IGnT A and B genes
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
cIGnT6 transfers multiple GlcNAc branches to long linear polylactosamines, a prerequisite for improving enzyme-assisted in vitro synthesis of a type of multivalent sialyl Lewis x glycans that are high affinity inhibitors of lymphocyte L-selectin, enzyme allows general polylactosamine synthesis