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Information on EC 2.4.1.149 - N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase and Organism(s) Mus musculus and UniProt Accession Q8K0J2
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EC Tree
2.4.1.149 N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferaseIUBMB Comments
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
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Word Map
- 2.4.1.149
- glycans
-
muscular
- dystrophy
- glycosyltransferases
- dystroglycanopathies
-
walker-warburg
-
bulb
-
olfactory
- alpha-dystroglycan
- gmppb
- keratan
-
laminin-binding
-
pathfinding
-
glomeruli
-
pomgnt1
- medicine
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
b3gnt3, b3gnt1, beta3gnt7, beta3gnt1, beta3gn-t7, beta-1,3-n-acetylglucosaminyltransferase-3, beta1-3 n-acetylglucosaminyltransferase-1, poly-n-acetyllactosamine extension enzyme, udp-glcnac:betagal beta-1,3-n-acetylglucosaminyltransferase 1, beta1,3-n-acetylglucosaminyltransferase-7, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-
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-
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Galbeta1-->4GlcNAc-R beta1-->3 N-acetylglucosaminyltransferase
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GnT-I
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i-GlcNAc transferase
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i-GlcNAcT
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IGnT
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N-acetyllactosamine beta(1-3)N-acetylglucosaminyltransferase
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poly-N-acetyllactosamine extension enzyme
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UDP-GlcNAc:Galbeta1-->4GlcNAcbeta-Rbeta1-->3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:Galbeta1-4Glc(NAc) beta-1,3-N-acetylglucosaminyltransferase
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UDP-GlcNAc:GalR, beta-D-3-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-acetyllactosaminide beta1-->3-acetylglucosaminyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY SOURCE
PATHWAYS
KEGG
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-, -, -
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R 3-beta N-acetylglucosaminyltransferase (configuration-inverting)
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
CAS REGISTRY NUMBER
COMMENTARY
85638-39-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + asialo alpha1-acid glycoprotein
?
-
sialic acid depleted alpha1-acid glycoprotein, 30% of the activity with neolactotetraosylceramide
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-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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i.e. neolactotetraosylceramide, GlcNAcT-2, synthesis in vitro of Ii core glycosphingolipids
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?
UDP-N-acetyl-D-glucosamine + lactosylceramide
?
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15% of the activity with neolactotetraosylceramide
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-
?
additional information
?
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beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
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?
additional information
?
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beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
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?
additional information
?
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B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
beta3GnT7 may play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues
-
-
?
additional information
?
-
-
B3gnt1 null females are fertile, the B3gnt1 null males are not able to sire litters at the expected rate when mated to either wild type or B3gnt1-null females
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
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requirement, Km: 1.25 mM, Mn2+ cannot be replaced by Mg2+, Zn2+, Cu2+ or Cd2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
detergent
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activates, reaction rate is optimal at detergent:protein ratio of 1.6 in Triton CF-54 or X-100, 1:3 in Nonidet P-40
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
breast cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
KLN205-MUC1, lung cancer cell line, higher expression of beta3GnT7 in cancer cell lines with low degrees of invasiveness
beta3GnT7 is most strongly expressed in the placenta at the later stages of gestation and in colon
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B3GN7_MOUSE
397
0
45379
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA from placenta encoding beta3GnT7 is cloned and partially characterized, open reading frame encodes a 397-amino acids protein
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
enzyme may be used as diagnostic marker of human cancer or as target molecule for the development of a new therapy
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kataoka, K.; Huh, N.h.
A novel beta1,3-N-acetylglucosaminyltransferase involved in invasion of cancer cells as assayed in vitro
Biochem. Biophys. Res. Commun.
294
843-848
2002
Homo sapiens (Q8NFL0), Homo sapiens, Mus musculus (Q8K0J2), Mus musculus, Mus musculus beta3GnT7 (Q8K0J2)
Basu, M.; Basu, S.
Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta1-3- and beta1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma
J. Biol. Chem.
259
12557-12562
1984
Mus musculus
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