Information on EC 2.4.1.147 - acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.1.147
-
RECOMMENDED NAME
GeneOntology No.
acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] = UDP + O3-[N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mucin core 3 and core 4 O-glycosylation
-
-
Mucin type O-Glycan biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-glucosamine:O3-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl/L-seryl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase
cf. EC 2.4.1.102 (beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase), EC 2.4.1.146 (beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase) and EC 2.4.1.148 (acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-mucin beta(1-3)-
-
-
-
-
beta3-N-acetylglucosaminyltransferase-6
-
-
beta3Gn-T6
-
-
beta3Gn-T6
-
beta3GnT-6
-
-
C3GnT
-
-
core 3 beta 1,3-N-acetylglucosaminyltransferase
-
-
core 3 beta3-GlcNAc-T
-
-
core 3 synthase
-
-
core 3 synthase
-
core 3beta-GlcNAc-transferase
-
-
-
-
core3 O-glycan synthase
-
-
core3 synthase
-
-
mucin core 3 beta3-GlcNAc-transferase
-
-
-
-
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
-
-
-
-
uridine diphosphoacetylglucosamine-mucin beta(1-3)-acetylglucosaminyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
87927-96-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme form beta3-Gn-T6
SwissProt
Manually annotated by BRENDA team
no activity in Canis familiaris
submaxillary glands
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-Ser/Thr
UDP + N-acetyl-beta-D-glucosaminyl-(1->)3-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + 2-azido-2-deoxy-alpha-D-galactosyl-benzyl
UDP + 3-O-(N-acetyl-beta-D-glucosaminyl)-2-azido-2-deoxy-alpha-D-galactosyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-nitrophenyl N-acetyl-alpha-D-galactosaminide
UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminide
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + 4-nitrophenyl N-acetyl-alpha-D-galactosaminide
UDP + 4-nitrophenyl N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminide
show the reaction diagram
recombinant protein, best substrate
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-alpha-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + asialofetuin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-asialofetuin
show the reaction diagram
recombinant protein, no activity with fetuin
-
?
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-alpha-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-alpha-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
recombinant protein, 2.4% activity compared to N-acetyl-beta-D-galactosaminyl-alpha-4-nitrophenyl
-
?
UDP-N-acetyl-D-glucosamine + bovine submaxillary gland mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of bovine submaxillary gland
show the reaction diagram
recombinant protein, no activity with fetuin
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-(CH2)2NHCO(CH2)3CO2CH3
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
-
best substrate
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-4-nitrophenyl
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-4-nitrophenyl
show the reaction diagram
recombinant protein, 15% activity compared to N-acetyl-beta-D-galactosaminyl-alpha-4-nitrophenyl
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis, important role in synthesis of mucin-type O-glycans in digestive organs
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-MUC1 mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-MUC1 mucin
show the reaction diagram
-
N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine is bound to the glycoprotein via serine or threonine residues, resulting in a core 3 structure
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
substrate specificity is significantly influenced by the structure of the aglycon group
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
activity is restricted to mucins from specific tissues, e.g. stomach, small intestine, and colon
N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine is bound to the glycoprotein via serine or threonine residues
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
R: polypeptide derived from mucin
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
R: polypeptide derived from mucin, no activity towards alpha-GalNAc residues of blood group A and the core 6 substrates, and beta-GalNAc residues of gangliosides, asialo-, lyso- and GalNAc-beta-1,4-(NeuAc-alpha-2,3-)Gal-beta-1,4-Glc-beta-1-1ceramide, i.e. GM2
N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosamine is bound to the glycoprotein via serine or threonine residues
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
no acceptor substrate: native ovine submaxillary mucin, containing sialyl-2,6-N-acetyl-D-galactosaminyl-groups
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
no acceptor substrate: native ovine submaxillary mucin, containing sialyl-2,6-N-acetyl-D-galactosaminyl-groups
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-galactosyl-ovine submaxillary gland mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosyl-mucin of ovine submaxillary gland
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + ovine submaxillary gland mucin
UDP + N-acetyl-beta-D-glucosaminyl-1,3-mucin of ovine submaxillary gland
show the reaction diagram
-
acceptor substrate is desialylated, degalactosylated, defucosylated
-
?
UDP-N-acetyl-D-glucosamine + phenyl 4-O-(2-deoxy-beta-D-lyxo-hexopyranosyl)-beta-D-glucopyranoside
UDP + ?
show the reaction diagram
-
45% of the activity with phenyl beta-lactoside. The enzyme forms a beta-D-GlcNAc-(1,3)-D-Gal glycosidic linkage
-
?
UDP-N-acetyl-D-glucosamine + phenyl 4-O-(3-deoxy-beta-D-xylo-hexopyranosyl)-beta-D-glucopyranoside
UDP + ?
show the reaction diagram
-
3% of the activity with phenyl beta-lactoside. The enzyme forms a beta-D-GlcNAc-(1,3)-D-Gal glycosidic linkage
-
?
UDP-N-acetyl-D-glucosamine + phenyl 4-O-(4-deoxy-beta-D-xylo-hexopyranosyl)-beta-D-glucopyranoside
UDP + ?
show the reaction diagram
-
15% of the activity with phenyl beta-lactoside. The enzyme forms a beta-D-GlcNAc-(1,3)-D-Gal glycosidic linkage
-
?
UDP-N-acetyl-D-glucosamine + phenyl 4-O-(6-deoxy-beta-D-galactopyranosyl)-beta-D-glucopyranoside
UDP + GlcNAcbeta(1-3)(6deoxy)Galbeta(1-4)Glcbeta-phenyl
show the reaction diagram
-
289% of the activity with phenyl beta-lactoside. The enzyme forms a beta-D-GlcNAc-(1,3)-D-Gal glycosidic linkage
-
?
UDP-N-acetyl-D-glucosamine + phenyl beta-lactoside
UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc-phenyl
show the reaction diagram
-
the enzyme forms a beta-D-GlcNAc-(1,3)-D-Gal glycosidic linkage
-
?
additional information
?
-
-
expression of the enzyme is closely regulated during differentiation and dedifferentiation. Rge expression of the enzyme dramatically decreased in cancerous tissues, and the changes to the core structures of O-glycans give rise to phenotypic changes of cancer cells, such as mobility and metastatic activity
-
-
-
additional information
?
-
-
C3GnT-deficient mice display a discrete, colonspecific reduction in Muc2 protein and increased permeability of the intestinal barrier. These mice are highly susceptible to experimental triggers of colitis and colorectal adenocarcinoma
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-galactosaminyl-Ser/Thr
UDP + N-acetyl-beta-D-glucosaminyl-(1->)3-N-acetyl-alpha-D-galactosaminyl-Ser/Thr
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
-
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis
-
-
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-galactosaminyl-alpha-R
UDP + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-beta-D-galactosaminyl-R
show the reaction diagram
Q6ZMB0
R: polypeptide derived from mucin, involved in mucin oligosaccharide biosynthesis, important role in synthesis of mucin-type O-glycans in digestive organs
-
-
additional information
?
-
-
expression of the enzyme is closely regulated during differentiation and dedifferentiation. Rge expression of the enzyme dramatically decreased in cancerous tissues, and the changes to the core structures of O-glycans give rise to phenotypic changes of cancer cells, such as mobility and metastatic activity
-
-
-
additional information
?
-
-
C3GnT-deficient mice display a discrete, colonspecific reduction in Muc2 protein and increased permeability of the intestinal barrier. These mice are highly susceptible to experimental triggers of colitis and colorectal adenocarcinoma
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
-
-
Mn2+
-
absolute requirement, 10-20 mM, cannot be replaced by Co2+, Ca2+, Mg2+ or Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Co2+
-
in the presence of Mn2+
deoxycholate
-
total inactivation
N-iodoacetamido-galactosamine-alpha-benzyl
-
colon homogenates, 50% inhibition at 2 mM
NaCl
-
slight inhibition at 0.1 M
PMSF
-
slight inhibition at 1 mM
Triton X-100
-
-
Triton X-100
-
above 0.5% v/v
Triton X-100
-
0.5% reduces activity by 60%
Triton X-114
-
slight stimulation
UDP
-
60% reduced activity at 10 mM
EDTA
-
-
additional information
-
no inhibition by 2-mercaptoethanol or N-acetyl-D-glucosamine
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NP 40
-
activation
Triton X-100
-
stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v
Triton X-100
-
stimulation, 0.1% v/v, inhibits at concentrations above 0.5% v/v
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2
N-acetyl-D-galactosaminyl-alpha-benzyl
-
-
3.2
N-acetyl-D-galactosaminyl-alpha-ovine submaxillary mucin
-
-
-
5
N-acetyl-D-galactosaminyl-alpha-phenyl
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00009
-
colon mucosa
0.00033
-
colon mucosa
0.00034
-
colon mucosa
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
-
assay at
7.2
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
assay at
37
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
expression of the enzyme is markedly down-regulated in gastric and colorectal carcinomas
Manually annotated by BRENDA team
normal colon, not colon cancer cells
Manually annotated by BRENDA team
-
in normal stomach and colon the enzyme is strongly expressed in the Golgi region of the epithelium
Manually annotated by BRENDA team
-
in normal stomach and colon the enzyme is strongly expressed in the Golgi region of the epithelium
Manually annotated by BRENDA team
additional information
-
-
Manually annotated by BRENDA team
additional information
-
absent from submaxillary glands
Manually annotated by BRENDA team
additional information
-
no activity in diverse cancer cell lines; tissue-specific expression
Manually annotated by BRENDA team
additional information
-
tissue-specific expression
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
in normal stomach and colon the enzyme is strongly expressed in the Golgi region of the epithelium
Manually annotated by BRENDA team
additional information
type II membrane protein
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 43000, amino acid sequence determination; x * 46600, recombinant FLAG-tagged fusion protein, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
recombinant FLAG-tagged fusion protein is glycosylated in insect cells
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is extremly unstable in solubilized form
-
repeated freezing and thawing results in total loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant from Sf21 insect cells as FLAG-tagged fusion protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in PC3 and LNCaP prostate cancer cell lines
-
expression in Spodoptera frugiperda Sf21 cells via baculovirus infection as soluble FLAG-tagged fusion protein
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
C3GnT-deficient mice display a discrete, colonspecific reduction in Muc2 protein and increased permeability of the intestinal barrier. These mice are highly susceptible to experimental triggers of colitis and colorectal adenocarcinoma
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
the enzyme would be a useful marker for distinguishing between benign adenomas and premalignant lesions