Information on EC 2.4.1.143 - alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.4.1.143
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RECOMMENDED NAME
GeneOntology No.
alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
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PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
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N-Glycan biosynthesis
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Various types of N-glycan biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:6-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor. Note that this enzyme acts after N-acetylglucosaminyltransferase I but before N-acetylglucosaminyltransferases III, IV, V and VI (click here for diagram).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
acetylglucosaminyltransferase II
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alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
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alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase
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GlcNAc-T II
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GlcNAcT-II
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GlcNAcTase-II
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GnT II
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GnT II
Q09326
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Gnt II-A
Q7ZZX7
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Gnt II-B
Q7ZZX7
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Mgat2
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Mgat2
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N-acetylglucosaminyltransferase II
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N-acetylglucosaminyltransferase II
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N-acetylglucosaminyltransferase II
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N-acetylglucosaminyltransferase II
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N-acetylglucosaminyltransferase II
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N-acetylglucosaminyltransferase-II
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N-acetylglucosaminyltransferases II
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N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
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UDP-GlcNAc:alpha6-D-mannoside beta1,2-N-acetylglucosaminyltransferase II
Q7ZZX7
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UDP-GlcNAc:mannoside alpha1-6 acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-alpha-D-mannoside beta1-2-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine-mannoside alpha1->6-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
105913-04-0
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202420-38-0
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91755-74-7
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91847-11-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Bm-N
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Manually annotated by BRENDA team
Bombyx mori Bm-N
Bm-N
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Manually annotated by BRENDA team
IZD-Mb-0503
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Manually annotated by BRENDA team
Mamestra brassicae IZD-Mb-0503
IZD-Mb-0503
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Manually annotated by BRENDA team
Mgat2 enzymatic activity, but not mRNA transcript levels, differ significantly among the three strains (PL/J C57BL/6 129/Sv)
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Manually annotated by BRENDA team
CHO-cells
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Manually annotated by BRENDA team
Sf9 and Sf12
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Manually annotated by BRENDA team
Gnt II-A; Gnt II-B
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
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part of N-glycan pathway
metabolism
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the enzyme functions at the N-glycan processing branch point and is one of the major factors determining the net outcome of the insect cell N-glycosylation pathway
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + 3-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)3-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + 3-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(3-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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-
-
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?
UDP-N-acetyl-D-glucosamine + 3-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)3-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
-
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?
UDP-N-acetyl-D-glucosamine + 4-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)4-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(4-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(4-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + 4-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
-
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?
UDP-N-acetyl-D-glucosamine + 6-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)6-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + 6-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(6-O-methyl-alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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-
-
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?
UDP-N-acetyl-D-glucosamine + 6-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)6-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
-
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-
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
-
-
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
-
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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R represents the remainder of the N-oligosaccharide core (n fucose), R: H or 1,4-(+/-)-fucose-1,6-N-acetylglucosaminyl-1-N-asparagine
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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best acceptor, high specificity
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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or 1,4-(+/-)-fucose-1,6-N-acetylglucosamine-pyridinylamine (with or without xylose in 1,2-beta-linkage to the beta-mannosyl residue)
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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acceptors are free and protein-matrix-bound glycans
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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UDP-N-acetyl-D-glucosamine cannot be replaced by UDP-N-acetylgalactosamine, UDPgalactose, UDPxylose, UDPglucose or GDPfucose, no acceptor substrates: overview
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-2-aminopyridine
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-2-aminopyridine
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-(alpha-D-mannosyl)-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
show the reaction diagram
Q7ZZX7
the enzyme catalyzes an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides
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?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
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essential for biosynthesis of complex N-linked oligosaccharides
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?
UDP-N-acetyl-D-glucosamine + betaGlc-O(CH2)7CH3
?
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + dideoxytetrasaccharide
UDP + dideoxypentasaccharide
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)-6-deoxy-Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)-6-deoxy-Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
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?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))-4-deoxy-Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))-4-deoxy-Manbeta-octyl
show the reaction diagram
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?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
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-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
UDP + GlcNAcbeta(1-2)Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
show the reaction diagram
Q7ZZX7
-
-
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1->6)[GlcNAcbeta(1->2)-Manbeta(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridyl-aminoside
?
show the reaction diagram
-
the enzyme can transfer a second N-acetylglucosamine residue to the product of the first transferase reaction but in an extremely slow reaction that is unlikely to be biologically relevant
-
-
?
additional information
?
-
-
the gene is alternatively spliced and developmmentally regulated
-
-
-
additional information
?
-
-
the enzyme is required for the chain initiation and elongation of the heparan sulfate backbone
-
-
-
additional information
?
-
-
the enzyme cannot use Man3GlcNAc2 and Manalpha(1->6)[Manbeta(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridyl-aminoside as substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
UDP + N-acetyl-beta-D-glucosaminyl 1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-R
show the reaction diagram
Q7ZZX7
the enzyme catalyzes an essential step in the biosynthetic pathway leading from high mannose to complex N-linked oligosaccharides
-
-
?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
show the reaction diagram
-
essential for biosynthesis of complex N-linked oligosaccharides
-
?
additional information
?
-
-
the gene is alternatively spliced and developmmentally regulated
-
-
-
additional information
?
-
-
the enzyme is required for the chain initiation and elongation of the heparan sulfate backbone
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Cd2+
-
activation, can replace Mn2+ to some extent, 0.5-1 mM
Mn2+
-
10-15 mM; requirement
Mn2+
-
2-3 mM; requirement
Mn2+
-
required for activity
additional information
-
Ca2+, Co2+, Cu2+ and Mg2+ cannot replace Mn2+
additional information
-
Ca2+, Co2+, Cu2+, Hg2+, Mg2+ or Zn2+ cannot replace Mn2+, at 1 or 2.5 mM each
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-deoxy-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
2-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
3-O-(4,4-azopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
3-O-(5-aminopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
3-O-pentyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
5-Hg-UDP
-
reversible
5-Hg-UDP-N-acetylglucosamine
-
reversible
6-deoxy-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
EDTA
-
complete inhibition in the presence of EDTA
GlcNAcbeta(1-2)Manalpha(1-3)Man-beta-octyl
-
-
N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-beta-D-mannosyl-octyl
-
-
O-(4,4-azo)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
irreversible inhibition
O-(4,4-azo)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
-
O-(5-amino)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
O-(5-iodoacetamido)pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
irreversible inhibition
O-pentyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
reversible inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Triton X-100
-
activation, 0.1%
Zwittergent 3-12
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.7
-
3-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.26
-
3-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.22
-
4-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.16
-
4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37C
0.26
-
4-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.25
-
6-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.2
-
6-O-methyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.55
-
alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-6-deoxy-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37C
0.13
-
alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl
-
37C
0.0166
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
pH 6.5
0.1
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
37C
0.19
-
alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
pH 6.5, 37C
0.55
-
Manalpha(1-6)(GlcNAcbeta(1-2)-6-deoxy-Manalpha(1-3))Manbeta-octyl
-
-
0.16
-
Manalpha(1-6)(GlcNAcbeta(1-2)4-O-methyl-Manalpha(1-3))Manbeta-octyl
-
-
0.48
-
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))-4-deoxy-Manbeta-octyl
-
-
0.13
-
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta-octyl
-
-
0.018
-
UDP-N-acetylglucosamine
-
pH 6.5
0.23
-
UDP-N-acetylglucosamine
-
pH 6.5, 37C
0.96
-
UDP-N-acetylglucosamine
-
pH 6.5, 37C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.13
-
2-deoxy-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
1
-
3-O-(4,4-azopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
2.5
-
3-O-(5-aminopentyl)-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
2.4
-
3-O-pentyl-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Man-beta-octyl
-
-
0.9
-
GlcNAcbeta(1-2)Manalpha(1-3)Man-beta-octyl
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.8
-
-
alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl as substrate
11.2
-
-
4-O-methyl-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl as substrate
20
-
-
-
21.4
-
-
5-deoxy-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-O-octyl as substrate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
6.5
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.7
8.1
-
the enzyme has an optimum pH of 6.7, with a broad shoulder of high activity from pH 7.1 to 8.1
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
gene is expressed mainly but not exclusively in
Manually annotated by BRENDA team
-
gene is expressed in the early stages of development mainly in the nervous system
Manually annotated by BRENDA team
-
reduction of N-acetylglucosaminyltransferase II in hyperkeratotic skin of ichthyosis patients
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
type II transmembrane topology
Manually annotated by BRENDA team
Q7ZZX7
enzyme exhibits a type II transmembrane protein topology with a putative N-terminal cytoplasmic tail of 9 amino acids followed by transmembrane domain of 18 residues, and a C-terminal luminal domain of 405 residues
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
51100
-
-
calculated from nucleotide sequence
52000
-
-
SDS-PAGE
56000
-
-
gel filtration, fusion protein
additional information
-
-
rat enzyme in crude extract exists in 2 forms, high-molecular weight form: above MW 200000, low-molecular weight form: MW 43000-48000, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 43000, x * 48000, rat, SDS-PAGE, one or both of these bands represent the enzyme
?
-
x * 42000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
Q7ZZX7
N-linked oligosaccharides
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
bovine serum albumin stabilizes during purification
-
Triton X-100 stabilizes
-
acetone precipitation, unstable to
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, at least 6 months in 20% glycerol, 10 mM EDTA and 0.1% Triton X-100
-
-20C, several weeks in 20% glycerol, 0.0005 mM DTT and 0.1% Triton X-100
-
0C, at least 1 week
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
IgG Sepharose resin column chromatography
-
solubilized with Triton X-100/NaCl, enzyme in crude extract exists in 2 molecular weight forms: purification of low-molecular weight form, affinity chromatography on Hg-UDP-N-acetylglucosamine-thiopropyl-Sepharose
-
solubilized with Triton X-100
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Spodoptera frugiperda Sf-9 cells
-
a transformed insect cell line (Tn5beta4GalT) constitutively expresses a mammalian beta-1,4-galactosyltransferase and human beta-1,2-N-acetylglucosaminyltransferase II under the control of an immediate-early promoter. The baculovirus-host system can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans
-
expressed in Spodoptera frugiperda and Trichoplusia ni cells by using a baculovirus expression vector
-
expression as fusion protein vHis-GlcNAc-TII in Spodoptera frugiperda Sf-9 cells
-
construction of a Gnt II-expressing alg3 mutant strain of Pichia pastoris. Engineering of an artificial glycosylation pathway in Pichia pastoris blocked in dolichol oligosaccharide assembly
Q09326
transient expression in COS-7 cells, linked in frame to a cDNA segment encoding the cleavable signal sequence of the human IL-2 receptor
-
expression in Escherichia coli
-
heterologous expression in insect cells
Q7ZZX7
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
limited enzyme expression after anti-CD3 stimulation
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
the baculoviris-host system constitutively expressing beta-1,4-galactosyltransferase and beta-1,2-N-acetylglucosaminyltransferase II can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N-glycans