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Information on EC 2.4.1.14 - sucrose-phosphate synthase and Organism(s) Oryza sativa and UniProt Accession Q0JGK4

for references in articles please use BRENDA:EC2.4.1.14
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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.14 sucrose-phosphate synthase
IUBMB Comments
Requires Mg2+ or Mn2+ for maximal activity . The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates . The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not . While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible . The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
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Oryza sativa
UNIPROT: Q0JGK4
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The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
sps, sucrose phosphate synthase, sucrose-phosphate synthase, sucrose phosphate synthetase, sps a, sucrose-p synthase, spsii, atsps, sps11, sucrose 6-phosphate synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SPS1
-
-
SPS11
-
-
sucrose 6-phosphate synthase
-
-
-
-
sucrose phosphate synthase
sucrose phosphate synthetase
-
-
-
-
sucrose phosphate-uridine diphosphate glucosyltransferase
-
-
-
-
sucrosephosphate-UDP glucosyltransferase
-
-
-
-
UDP-glucose-fructose-phosphate glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-fructose phosphate glucosyltransferase
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-06-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
stimulates enzyme activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glucose-6-phosphate
Mg2+
-
stimulates enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.5 - 4.1
D-fructose 6-phosphate
3.6 - 4.2
UDP-glucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.9
-
purified enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
germinated
Manually annotated by BRENDA team
additional information
-
no activity in roots
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
knockdown and knockout mutants of isoform SPS1 show a 29-46% reduction in enzyme activity in the leaves, but the carbohydrate content in the leaves and plant growth are not significantly different from those of wild type plants. In a double knockout mutant of SPS1 and SPS11, an 84% reduction in leaf enzyme activity results in higher starch accumulation in the leaves than in the wild type leaves. However, the double knockout mutant plants grow normally
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
116000
420000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 116000, gel filtration, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
SPS-I loses more activity during purification than SPS-II
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene SPS, qualitative and quantitative realtime PCR analysis, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
diagnostics
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Salerno, G.L.; Pagnussat, G.C.; Pontis, H.G.
Studies on sucrose-phosphate synthase from rice leaves
Cell. Mol. Biol.
44
407-416
1998
Oryza sativa
Manually annotated by BRENDA team
Pattanayak, D.
Higher plant sucrose-phosphate synthase: structure, function and regulation
Indian J. Exp. Biol.
37
523-529
1999
Beta vulgaris, Glycine max, Oryza sativa, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Pagnussat, G.C.; Curatti, L.; Salerno, G.L.
Rice sucrose-phosphate synthase: identification of an isoform specific for heterotrophic tissues with distinct metabolite regulation from the mature leaf enzyme
Physiol. Plant.
108
337-344
2000
Oryza sativa
-
Manually annotated by BRENDA team
Lutfiyya, L.L.; Xu, N.; DOrdine, R.L.; Morrell, J.A.; Miller, P.W.; Duff, S.M.G.
Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants
J. Plant Physiol.
164
923-933
2007
Actinidia chinensis, Actinidia deliciosa, Synechocystis sp., Arabidopsis thaliana, Musa acuminata, Beta vulgaris, Brassica rapa, Vicia faba, Citrus unshiu, Hordeum vulgare, Ipomoea batatas, Medicago sativa, Solanum lycopersicum, Mangifera indica, Nicotiana tabacum, Nostoc punctiforme, Oryza sativa, Pinus pinaster, Prochlorococcus marinus, Saccharum officinarum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Viscum album, Zea mays, Gloeobacter violaceus, Thermosynechococcus vestitus, Pirellula sp., Craterostigma plantagineum, Oncidium Goldiana, Synechococcus marinus
Manually annotated by BRENDA team
Jiang, L.; Yang, L.; Zhang, H.; Guo, J.; Mazzara, M.; Van den Eede, G.; Zhang, D.
International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified rice
J. Agric. Food Chem.
57
3525-3532
2009
Oryza sativa (A2WYE9), Oryza sativa
Manually annotated by BRENDA team
Hashida, Y.; Hirose, T.; Okamura, M.; Hibara, K.I.; Ohsugi, R.; Aoki, N.
A reduction of sucrose phosphate synthase (SPS) activity affects sucrose/starch ratio in leaves but does not inhibit normal plant growth in rice
Plant Sci.
253
40-49
2016
Oryza sativa
Manually annotated by BRENDA team