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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Glycine max and UniProt Accession P13708
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2.4.1.13 sucrose synthaseIUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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Word Map
- 2.4.1.13
- invertase
- starch
- maize
-
sink
- cultivar
-
pyrophosphorylase
- grain
- endosperm
- crop
- seedling
- cellulose
- potato
- cotton
- tuber
- drought
- phloem
-
sucrose-phosphate
- fructokinase
- kernel
- hexoses
- hexokinase
- agpase
-
3.2.1.26
-
anthesis
- callose
- hirsutum
- sugarcane
-
photosynthate
- panicle
- fructans
-
granule-bound
-
gossypium
-
grain-filling
-
photoassimilate
- adpglucose
- spikelet
-
source-sink
-
boll
- saccharum
- synthesis
- stachyose
-
amyloplasts
- taproot
- agriculture
The taxonomic range for the selected organisms is: Glycine max
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, susy1, mtsucs1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
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sucrose synthetase
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sucrose-UDP glucosyltransferase
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-
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sucrose-uridine diphosphate glucosyltransferase
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Sus
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SuSy
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-
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UDP-glucose-fructose glucosyltransferase
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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uridine diphosphoglucose-fructose glucosyltransferase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY
9030-05-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
the reaction is catalyzed at high pH values
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-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
the reaction is catalyzed at high pH values
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-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
Ser11 is primary phosphorylation site, phosphorylation does not affect kinetics but partitioning between membrane and cytosol
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additional information
-
Ser11 is primary phosphorylation site, phosphorylation does not affect kinetics but partitioning between membrane and cytosol
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Mg2+
additional information
-
no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
quercetin
the enzyme is tolerant to quercetin up to a concentration of 1.5 mM
additional information
-
not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate
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additional information
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not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.7
D-fructose
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
21.5
D-fructose
with UDP-alpha-D-glucose as cosubstrate, at pH 7.5 and 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8.2
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about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage
7.5 - 10
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about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SUS_SOYBN
805
0
92244
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
N-terminal truncation, phosphorylations still occurs, but weakly
additional information
-
N-terminal truncation, phosphorylations still occurs, but weakly
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
a biocatalytic cascade of polyphosphate kinase and sucrose synthase is developed for synthesis of nucleotide-activated derivatives of glucose
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Morell, M.; Copeland, L.
Sucrose synthase of soybean nodules
Plant Physiol.
78
149-154
1985
Glycine max
Thummler, F.; Verma, D.P.S.
Nodulin-100 of soybean is the subunit of sucrose synthase regulated by the availability of free heme in nodules
J. Biol. Chem.
262
14730-14736
1987
Glycine max
Zhang, X.Q.; Lund, A.A.; Sarath, G.; Cerny, R.L.; Roberts, D.M.; Chollet, R.
Soybean nodule sucrose synthase (nodulin-100): further analysis of its phosphorylation using recombinant and authentic root-nodule enzymes
Arch. Biochem. Biophys.
371
70-82
1999
Glycine max (P13708), Glycine max
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Citrus unshiu (Q9SLY2), Citrus x paradisi, Coffea arabica (Q0E7D4), Daucus carota (O49845), Eucalyptus grandis (Q00P15), Eucalyptus grandis (Q00P16), Glycine max (P13708), Glycine max, Gossypium hirsutum (Q9XGB7), Gossypium hirsutum, Oryza sativa (P31924), Pisum sativum (O24301), Pisum sativum (O81610), Pisum sativum (Q9AVR8), Pisum sativum (Q9T0M9), Solanum lycopersicum (P49037), Solanum tuberosum (Q84T18), Vicia faba (P31926), Zea mays
Pei, J.; Sun, Q.; Gu, N.; Zhao, L.; Fang, X.; Tang, F.; Cao, F.
Production of isoorientin and isovitexin from luteolin and apigenin using coupled catalysis of glycosyltransferase and sucrose synthase
Appl. Biochem. Biotechnol.
190
601-615
2020
Glycine max (P13708), Glycine max
Schmoelzer, K.; Gutmann, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Sucrose synthase A unique glycosyltransferase for biocatalytic glycosylation process development
Biotechnol. Adv.
34
88-111
2016
Anabaena sp., Arabidopsis thaliana, Beta vulgaris, Vicia faba, Helianthus tuberosus, Hordeum vulgare, Ipomoea batatas, Manihot esculenta, Nitrosomonas europaea, Oryza sativa, Vigna radiata, Pisum sativum, Prunus persica, Pyrus pyrifolia, Saccharum sp., Solanum tuberosum, Zea mays, Thermosynechococcus vestitus, Acidithiobacillus caldus, Solanum chmielewskii, Glycine max (P13708)
Gutmann, A.; Lepak, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Glycosyltransferase cascades for natural product glycosylation Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP
Biotechnol. J.
12
1600557
2017
Acidithiobacillus caldus (A0A059ZV61), Acidithiobacillus caldus, Glycine max (P13708), Glycine max, Acidithiobacillus caldus ATCC 51756 (A0A059ZV61)
Pei, J.; Chen, A.; Zhao, L.; Cao, F.; Li, X.; Xiao, W.
Synergistic catalysis of glycosyltransferase and sucrose synthase to produce isoquercitrin through glycosylation of quercetin
Chem. Nat. Compd.
55
453-457
2019
Glycine max (P13708)
-
Kulmer, S.; Gutmann, A.; Lemmerer, M.; Nidetzky, B.
Biocatalytic cascade of polyphosphate kinase and sucrose synthase for synthesis of nucleotide-activated derivatives of glucose
Adv. Synth. Catal.
359
292-301
2017
Acidithiobacillus caldus (A0A059ZV61), Glycine max (P13708), Acidithiobacillus caldus ATCC 51756 (A0A059ZV61)
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EXTERNAL LINKS (specific for EC-Number 2.4.1.13)
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