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Information on EC 2.4.1.13 - sucrose synthase and Organism(s) Pisum sativum and UniProt Accession O24301

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.13 sucrose synthase
IUBMB Comments
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) .
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This record set is specific for:
Pisum sativum
UNIPROT: O24301
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The taxonomic range for the selected organisms is: Pisum sativum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
sucrose synthase, sucrose synthetase, mtsucs1, susy1, rsus3, sucrose synthase 1, psnsusy2, susy2, sucrose synthase 2, sucrose synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glucosyltransferase, uridine diphosphoglucose-fructose
-
-
-
-
sucrose synthetase
-
-
-
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sucrose-UDP glucosyltransferase
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-
-
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sucrose-uridine diphosphate glucosyltransferase
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-
-
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SUS1
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isoform
SUS2
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isoform
SUS3
-
isoform
UDP-glucose-fructose glucosyltransferase
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-
-
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UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
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-
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uridine diphosphoglucose-fructose glucosyltransferase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
?
ADP + sucrose
ADP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
ADP-alpha-D-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
CDP + sucrose
CDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
CDP-alpha-D-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
dTDP + sucrose
dTDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dTDP-alpha-D-glucose + D-fructose
dTDP + sucrose
show the reaction diagram
-
-
-
-
r
dUDP + sucrose
dUDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
dUDP-alpha-D-glucose + D-fructose
dUDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP + sucrose
GDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
GDP-alpha-D-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
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-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
the reaction is catalyzed at high pH values
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-
r
UDP + sucrose
UDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
?
NDP + sucrose
NDP-alpha-D-glucose + D-fructose
show the reaction diagram
-
the reaction is catalyzed at low pH values
-
-
r
NDP-alpha-D-glucose + D-fructose
NDP + sucrose
show the reaction diagram
-
the reaction is catalyzed at high pH values
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no ionic activator requirement
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
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at 0.4 mM
Cu2+
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at 0.4 mM
Fe3+
-
0.4 mM
Hg2+
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0.4 mM
iodoacetic acid
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partially reversible by GSH or DTT
N-ethylmaleimide
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partially reversible by GSH or DTT
p-hydroxymercuribenzoate
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partially reversible by GSH or DTT
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
nitrate
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50% activation at 3-5 mM, exogenous nitrate at 14.2 mM absorbed in the form of KNO3 and Ca(NO3)2 during 10-20 days exponentially activates the enzyme in the roots by 22-100% as compared with plants grown on nitrogen-free medium. Under low light, nitrate can not activate sucrose synthase
UDP-D-xylose
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activation of epicotyl and cotyledon isozymes
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.1 - 20.9
D-fructose
32.1 - 67.5
sucrose
0.082 - 0.15
UDP
0.058 - 0.21
UDP-alpha-D-glucose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state
Manually annotated by BRENDA team
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cotyledon, epicotyl and root of etiolated seedling
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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sucrose synthase is the main sucrose breakdown enzyme in plant sink tissues. Sucrose synthase levels do not limit or regulate carbon transfer in the arbuscular mycorrhizal symbiosis
additional information
-
roots of plants deficient in sucrose synthase root isozyme are colonized successfully by arbuscular myccorhizal fungi in a similar levelthan the wild-type plant roots
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SUS2_PEA
809
0
92208
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
410000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
at least 3 isozymes
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native enzyme partially by gel filtration
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Ni-NTA column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
DNA and amino acid sequence determination and analysis, phylogenetic analysis
expressed in Escherichia coli BL21(DE3) cells
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isoforms Sus1-3
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity increases by 20-200% for 10-20 days of plant growth in presence of (NH4)2SO4 as compared with the roots of plants growing without nitrogen, induction is higher under illumination by sunlight
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sung, H.Y.; Su, J.C.
Sucrose synthetase. II. Sucrose synthetase isozymes of pea seedlings - purification and general properties
J. Chin. Biochem. Soc.
6
22-37
1977
Pisum sativum
-
Manually annotated by BRENDA team
Barratt, D.H.; Barber, L.; Kruger, N.J.; Smith, A.M.; Wang, T.L.; Martin, C.
Multiple, distinct isoforms of sucrose synthase in pea
Plant Physiol.
127
655-664
2001
Pisum sativum
Manually annotated by BRENDA team
Dejardin, A.; Rochat, C.; Maugenest, S.; Boutin, J.P.
Purification, characterization, and physiological role of sucrose synthase in the pea seed coat (Pisum sativum)
Planta
201
128-137
1997
Pisum sativum
Manually annotated by BRENDA team
Marino, D.; Hohnjec, N.; Kuester, H.; Moran, J.F.; Gonzalez, E.M.; Arrese-Igor, C.
Evidence for transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state
Mol. Plant Microbe Interact.
21
622-630
2008
Pisum sativum (O24301), Pisum sativum
Manually annotated by BRENDA team
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana (F4K5W8), Arabidopsis thaliana (P49040), Arabidopsis thaliana (Q00917), Citrus unshiu (Q9SLY2), Citrus x paradisi, Coffea arabica (Q0E7D4), Daucus carota (O49845), Eucalyptus grandis (Q00P15), Eucalyptus grandis (Q00P16), Glycine max (P13708), Glycine max, Gossypium hirsutum (Q9XGB7), Gossypium hirsutum, Oryza sativa (P31924), Pisum sativum (O24301), Pisum sativum (O81610), Pisum sativum (Q9AVR8), Pisum sativum (Q9T0M9), Solanum lycopersicum (P49037), Solanum tuberosum (Q84T18), Vicia faba (P31926), Zea mays
Manually annotated by BRENDA team
Yarnes, S.; Sengupta-Gopalan, C.
Sucrose synthase levels do not limit or regulate carbon transfer in the arbuscular mycorrhizal symbiosis
J. Plant Interact.
4
113-117
2009
Pisum sativum
-
Manually annotated by BRENDA team
Bruskova, R.; Nikitin, A.; Satskaya, M.; Izmailov, S.
Effect of nitrate on pea sucrose synthase
Russ. J. Plant Physiol.
56
74-79
2009
Pisum sativum
-
Manually annotated by BRENDA team
Nikitin, A.; Bruskova, R.; Andreeva, T.; Izmailov, S.
Effect of ammonia on sucrose synthase in pea roots
Russ. J. Plant Physiol.
57
69-73
2010
Pisum sativum
-
Manually annotated by BRENDA team
Schmoelzer, K.; Gutmann, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
Sucrose synthase A unique glycosyltransferase for biocatalytic glycosylation process development
Biotechnol. Adv.
34
88-111
2016
Anabaena sp., Arabidopsis thaliana, Beta vulgaris, Vicia faba, Helianthus tuberosus, Hordeum vulgare, Ipomoea batatas, Manihot esculenta, Nitrosomonas europaea, Oryza sativa, Vigna radiata, Pisum sativum, Prunus persica, Pyrus pyrifolia, Saccharum sp., Solanum tuberosum, Zea mays, Thermosynechococcus vestitus, Acidithiobacillus caldus, Solanum chmielewskii, Glycine max (P13708)
Manually annotated by BRENDA team