Information on EC 2.4.1.13 - sucrose synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.4.1.13
-
RECOMMENDED NAME
GeneOntology No.
sucrose synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
non-pathway related
-
-
Starch and sucrose metabolism
-
-
sucrose degradation II (sucrose synthase)
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
CAS REGISTRY NUMBER
COMMENTARY hide
9030-05-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
green alga
-
-
Manually annotated by BRENDA team
kangaroo paw
-
-
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
Mokara Yellow
-
-
Manually annotated by BRENDA team
2 genotypes ICPL 84023 and ICP 301 tolerant to waterlogging stress, and 2 genotypes ICP 7035 and Pusa 207 susceptible to waterlogging stress
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
inbred line M01-3
-
-
Manually annotated by BRENDA team
carnation
-
-
Manually annotated by BRENDA team
Gladiolus sp.
gladiolus
-
-
Manually annotated by BRENDA team
sweet potato, cv. Okinava no. 100
-
-
Manually annotated by BRENDA team
morning-glory
-
-
Manually annotated by BRENDA team
Leleba oldhami
bamboo
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
banana
-
-
Manually annotated by BRENDA team
quaking aspen
-
-
Manually annotated by BRENDA team
almond
-
-
Manually annotated by BRENDA team
var. Rehder var. culta Rehder cv. Hosui, isoforms SS I, SS II
-
-
Manually annotated by BRENDA team
L. Moench
-
-
Manually annotated by BRENDA team
sturt pea
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
-
roots of plants deficient in sucrose synthase root isozyme are colonized successfully by arbuscular myccorhizal fungi in a similar levelthan the wild-type plant roots
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
IDP-glucose + D-fructose
IDP + sucrose
show the reaction diagram
-
-
-
-
?
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-alpha-D-galactose + D-fructose
UDP + sucrose
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-glucose + 1-deoxy-1-fluoro-fructose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 175% of the activity with D-fructose, 100% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
UDP-alpha-D-glucose + D-glucose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-lyxose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 150% of the activity with D-fructose, 48% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-mannose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 75% of the activity with D-fructose, 40% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-ribose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 7% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-ribulose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 24% of the activity with D-fructose, no activity with enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-tagatose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 43% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-talose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 117% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-xylose
?
show the reaction diagram
UDP-alpha-D-glucose + L-arabinose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 490% of the activity with D-fructose, 36% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-galactose
?
show the reaction diagram
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + L-glucose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 34% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-mannose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 59% of the activity with D-fructose, 8% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-rhamnose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 52% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-sorbose
?
show the reaction diagram
the recombinant enzyme expressed in Escherichia coli shows 96% of the activity with D-fructose, 55% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucuronic acid + D-fructose
?
show the reaction diagram
-
32% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-xylose + D-fructose
?
show the reaction diagram
-
39% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
UDP-N-acetyl-alpha-D-galactosamine + D-fructose
UDP + beta-D-fructofuranosyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + D-fructose
UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
show the reaction diagram
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
Q01390
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P10691
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
5-deoxy-beta-D-fructose
-
competitive to D-fructose
5-deoxy-D-fructose
10% inhibition
6-Dimethylaminopurine
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
actin
-
0.001 mM, 17% inhibition of SuSy1
Ag+
-
at 0.4 mM
Arbutin
-
no enzyme activity at 5 mM
Co2+
-
0.05 mM, 20% loss of activity
D-fructose
Fe2+
-
no activity at 1 mM
Fe3+
-
0.4 mM
fructose
glucose
GTP
-
sucrose cleavage
imidazole
-
-
iodoacetic acid
N-ethylmaleimide
okadaic acid
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
p-hydroxymercuribenzoate
Salicine
-
kinetics
Sucrose
UDP-glucose
UDPglucose
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
abscisic acid
-
as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
actin
-
activity of SuSy2 in breakdown direction is stimulated by 60%
allantoin
-
activation, sucrose synthesis
Ca2+
-
only for sucrose synthesis
fructose 2,6-bisphosphate
-
stimulates SuSy2 in presence of actin
Mn2+
-
2-4fold activation of sucrose synthesis, inhibition of cleavage
nitrate
-
50% activation at 3-5 mM, exogenous nitrate at 14.2 mM absorbed in the form of KNO3 and Ca(NO3)2 during 10-20 days exponentially activates the enzyme in the roots by 22-100% as compared with plants grown on nitrogen-free medium. Under low light, nitrate can not activate sucrose synthase
Sucrose
-
exogenous, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
UDP-D-xylose
-
activation of epicotyl and cotyledon isozymes
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 3.8
ADP
0.44 - 3.7
CDP
1.05 - 11.7
D-fructose
0.012
dTDP
-
pH 7.6, 30C
0.98 - 40
fructose
0.17 - 4.5
GDP
0.0916 - 303
Sucrose
0.65
TDP
-
-
0.00191 - 1.25
UDP
0.6 - 1.4
UDP-alpha-D-glucose
0.77
UDP-GlcNAc
-
pH 8.0, 30C
0.05 - 25
UDP-glucose
11.5
UDPfructose
-
isozyme SS1
0.012 - 6
UDPglucose
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.97 - 39.5
Sucrose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
5-deoxy-beta-D-fructose
-
-
0.015
Cu2+
-
-
1.8 - 35.9
D-fructose
2.23 - 17.2
fructose
4.3 - 53
glucose
1 - 1.5
Mn2+
-
sucrose cleavage
0.035
Ni2+
-
-
227
Sucrose
-
substrate inhibition
0.086 - 0.11
UDP
0.104 - 2.3
UDP-glucose
0.025
Zn2+
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
Solanum tuberosum
P10691
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
-
value about, degrading direction, leaf, 20 days after anthesis; value about, synthezising direction, leaf, 2 days before anthesis
0.004167
-
value about, synthezising direction, leaf, 8 days after anthesis
0.005
-
value about, degrading direction, leaf, 16 days after anthesis; value about, synthezising direction, leaf, 16 days after anthesis
0.0067
-
value about, degrading direction, leaf, 12 days after anthesis; value about, synthezising direction, leaf, 20 days after anthesis; value about, synthezising direction, mesocarp tissue, 16 days after anthesis
0.0083
-
value about, degrading direction, leaf, 4 days and 8 days after anthesis; value about, synthezising direction, mesocarp tissue, 20 days after anthesis; value about, synthezising direction, mesocarp tissue, 2 days before anthesis
0.0125
-
value about, degrading direction, leaf, 2 days before anthesis; value about, synthezising direction, mesocarp tissue, 8 days after anthesis
0.013
-
value about, synthezising direction, mesocarp tissue, day of anthesis
0.1
-
value about, degrading direction, mesocarp tissue, 2 days before anthesis and day of anthesis
0.1167
-
value about, degrading direction, mesocarp tissue, 4 days after anthesis
0.183
-
value about, degrading direction, mesocarp tissue, 8 days after anthesis
0.2083
-
value about, synthezising direction, mesocarp tissue, 12 to 20 days after anthesis
0.3
crude extract, recombinant enzyme in Saccharomyces cerevisiae
0.33
-
SUS activity in rice grain untreated during grain filling
0.45
-
SUS activity in rice grain treated with abscisic acid during grain filling
0.53
-
SUS activity in rice grain treated with sducrose and abscisic acid during grain filling
0.66
-
SUS activity in rice grain treated with sucrose during grain filling
0.89
-
-
3.54
-
-
3.85
-
-
4.35
-
-
4.5
purified recombinant enzyme, expressed in Escherichia coli
6
-
isozyme SS2
6.8
-
wild-type line ICCV 96029
6.9
purified recombinant enzyme, expressed in Saccharomyces cerevisiae
8.34
-
-
9.5
-
wild-type line 96030
10
-
isozyme SS1
15.1
-
-
128
-
sucrose cleavage
180
-
sucrose synthesis
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
assay at
6 - 6.5
-
sucrose cleavage
6.4
-
sucrose cleavage
6.5 - 7
-
in 0.2 M HEPES-NaOH, varies with buffer
6.6
-
calculated from sequence, enzyme form CaSUS2
7 - 9.5
-
broad, sucrose synthesis
7
-
sucrose cleavage
7.6
-
cleavage of sucrose
9.5
-
sucrose synthesis
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
-
about half-maximal activity at pH 5.0 and 10.0, sucrose synthesis
5 - 8
-
about half-maximal activity at pH 5.0 and 8.0, sucrose cleavage
5 - 8.2
-
about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage
5.5 - 9
-
about 80% of maximal activity at pH 5.5 and about half-maximal activity at pH 9.0, sucrose cleavage
5.6 - 10
-
about half-maximal activity at pH 5.6 and about 90% of maximal activity at pH 10.0, sucrose synthesis
6.2 - 6.6
-
96% of maximal activity at pH 6.2 and 6.6
7.5 - 10
-
about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis
9.5
-
about 10-15% of maximum activity; about 10-15% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 50
pH 6.5, cleavage of sucrose, SuSyII
45
pH 7.5, synthesis of sucrose, SuSyI
50
pH 6.5, cleavage of sucrose, SuSyI. Synthesis of sucrose, SuSy II
50 - 60
-
in 0.2 M Hepes-NaOH, varies with buffer
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
-
calculated from sequence, enzyme form CaSUS1
additional information
-
total protein extract shows two cross-reacting spots at 88000 Da with a pI of 5.5 to 6.0
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
CaSUS1 expression is barely detectable in young flower buds and leaves. Transcripts of this isoform are observed in old flower buds and roots, and accumulates to a high level in stems
Manually annotated by BRENDA team
-
at least one form of SuSy present in young tissue is absent, or present below detection limits, in mature culm tissue
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
expressed at high levels during secondary cell wall synthesis in fiber
Manually annotated by BRENDA team
-
SUS1 is the predominant isoform of SUS associated with microsomes isolated from the base of the maize leaf elongation zone and from kernels at 20 and 30 days after pollination. SUS2 exists predominantly as a hetero-oligomer with SUS1 in kernels; SUS2 is particularly abundant in kernels at various pollination stages; SUSSH1 is predominant in developing kernels
Manually annotated by BRENDA team
-
; mRNA levels of CaSUS2 is barely detectable in young pericarp, but increased towards the ripening of pericarp tissues
Manually annotated by BRENDA team
-
mRNA levels of CaSUS2 is barely detectable in young pericarp
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
preferentially localized in the endopolyploid outer cells
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
-
high expression of Msus1 mRNA
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus. Within the silique wall, SUS localizes specifically to the companion cells, indicating that SUS activity may be required to provide energy for phloem transport activities in the silique wall
Manually annotated by BRENDA team
additional information