Information on EC 2.4.1.13 - sucrose synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.4.1.13
-
RECOMMENDED NAME
GeneOntology No.
sucrose synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
-
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
Leleba oldhami
-
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
-
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
-
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
mechanism
-
NDP-glucose + D-fructose = NDP + sucrose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
Starch and sucrose metabolism
-
sucrose degradation II (sucrose synthase)
-
SYSTEMATIC NAME
IUBMB Comments
NDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
Although UDP is generally considered to be the preferred nucleoside diphosphate for sucrose synthase, numerous studies have shown that ADP serves as an effective acceptor molecule to produce ADP-glucose [3-9]. Sucrose synthase has a dual role in producing both UDP-glucose (necessary for cell wall and glycoprotein biosynthesis) and ADP-glucose (necessary for starch biosynthesis) [10].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CaSUS1
-
-
CaSUS2
-
-
glucosyltransferase, uridine diphosphoglucose-fructose
-
-
-
-
LjSUS3
-
accounts for more than 60% of the total SUS transcripts
Msus1
Arachnis hookeriana x Ascocenda Madame Kenny
-
-
mtSUS
-
-
SBSS1
-
-
SH1
-
-
SH1
-
two isoforms of sucrose synthase(SH1 and SUS1) are encoded by the Shrunken1 (Sh1) and Sucrose synthase1 (Sus1)
sucrose synthase
P49040, Q00917, Q94CC8, Q9FHU4, Q9FX32, Q9M111
-
sucrose synthase
-
-
sucrose synthase
-
-
sucrose synthase 1
P49040
-
sucrose synthase 1
-
-
sucrose synthase 1
P10691
-
sucrose synthase 1
-
-
sucrose synthase 2
Q00917
-
sucrose synthase 4
Q7Y078
-
sucrose synthetase
-
-
-
-
sucrose-UDP glucosyltransferase
-
-
-
-
sucrose-uridine diphosphate glucosyltransferase
-
-
-
-
Sus
-
-
-
-
Sus
O49845
-
Sus
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
-
Sus
Q9T0M9
-
Sus
Q84T18
-
Sus
-
-
SUS-SH1
-
-
SUS1
-
two isoforms of sucrose synthase(SH1 and SUS1) are encoded by the Shrunken1 (Sh1) and Sucrose synthase1 (Sus1)
SUS2
-
-
sus4
Q7Y078
-
SuSy
-
-
-
-
SuSy
Q7Y078
-
SuSy
P31926
-
SuSy1
P10691
-
SuSyI
Q6SJP5
-
SuSyII
Q6SJP5
-
UDP-D-Glc:D-Fru 2-alpha-glucosyltransferase
-
-
UDP-glucose-fructose glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose 2-alpha-D-glucosyltransferase
-
-
uridine diphosphoglucose-fructose glucosyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-05-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain PCC 7119, two isoforms
-
-
Manually annotated by BRENDA team
kangaroo paw
-
-
Manually annotated by BRENDA team
-
Q9FHU4
UniProt
Manually annotated by BRENDA team
ecotype Columbia
-
-
Manually annotated by BRENDA team
isoform SUS1
UniProt
Manually annotated by BRENDA team
isoform Sus2
UniProt
Manually annotated by BRENDA team
isoform Sus3
UniProt
Manually annotated by BRENDA team
sucrose synthase 1
UniProt
Manually annotated by BRENDA team
sucrose synthase 2
UniProt
Manually annotated by BRENDA team
syncytia induced by nematode Heterodera schachtii
P49040, Q00917, Q94CC8, Q9FHU4, Q9FX32, Q9M111
UniProt
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
Mokara Yellow
-
-
Manually annotated by BRENDA team
SBSS2
SwissProt
Manually annotated by BRENDA team
sugar beet
-
-
Manually annotated by BRENDA team
2 genotypes ICPL 84023 and ICP 301 tolerant to waterlogging stress, and 2 genotypes ICP 7035 and Pusa 207 susceptible to waterlogging stress
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
Sona, a small-seeded desi cultivar, and Kaniva, a large-seeded kabuli cultivar
-
-
Manually annotated by BRENDA team
wild-type lines ICCV 96029 and ICCV 96030
-
-
Manually annotated by BRENDA team
sucrose synthase 1
UniProt
Manually annotated by BRENDA team
inbred line M01-3
-
-
Manually annotated by BRENDA team
cucumber, isoforms SSI, SSII
-
-
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
gene SuSy1
UniProt
Manually annotated by BRENDA team
gene SuSy3
UniProt
Manually annotated by BRENDA team
Gladiolus sp.
gladiolus
-
-
Manually annotated by BRENDA team
Merr cv. Williams
-
-
Manually annotated by BRENDA team
recombinant enzyme
UniProt
Manually annotated by BRENDA team
soy bean, cv. Prize
-
-
Manually annotated by BRENDA team
isoform SusC
UniProt
Manually annotated by BRENDA team
var. Coker 315
-
-
Manually annotated by BRENDA team
Jerusalem artichoke, isoforms A, B
-
-
Manually annotated by BRENDA team
isoform SS1
UniProt
Manually annotated by BRENDA team
isoform SS3
UniProt
Manually annotated by BRENDA team
isoform SS4
UniProt
Manually annotated by BRENDA team
sweet potato, cv. Okinava no. 100
-
-
Manually annotated by BRENDA team
morning-glory
-
-
Manually annotated by BRENDA team
Leleba oldhami
bamboo
-
-
Manually annotated by BRENDA team
gene MtSucS1
-
-
Manually annotated by BRENDA team
Jemalong A17
-
-
Manually annotated by BRENDA team
banana
-
-
Manually annotated by BRENDA team
3 isoforms
-
-
Manually annotated by BRENDA team
4 isozymes
-
-
Manually annotated by BRENDA team
gene SUS1; var. japonica, cv. Nipponbare, gene SUS1
UniProt
Manually annotated by BRENDA team
gene SUS2; var. japonica, cv. Nipponbare, gene SUS2
UniProt
Manually annotated by BRENDA team
gene SUS3; var. japonica, cv. Nipponbare, gene SUS3
UniProt
Manually annotated by BRENDA team
gene SUS4; var. japonica, cv. Nipponbare, gene SUS4
UniProt
Manually annotated by BRENDA team
gene SUS6; var. japonica, cv. Nipponbare, gene SUS6
UniProt
Manually annotated by BRENDA team
genes SUS5; var. japonica, cv. Nipponbare, gene SUS5
Q0JE91
UniProt
Manually annotated by BRENDA team
rice, var. Nihonbare
-
-
Manually annotated by BRENDA team
sucrose synthase 1
UniProt
Manually annotated by BRENDA team
two cultivars, cv. Shanyou 63 and Liangyoupeijiu, Peiai 64S/93-11, an indica/indica F1 hybrid
-
-
Manually annotated by BRENDA team
var. japonica, cv. Nipponbare, genes SUS1-4
-
-
Manually annotated by BRENDA team
3 isoforms
-
-
Manually annotated by BRENDA team
cv. Truzhenik
-
-
Manually annotated by BRENDA team
nodule-enhanced sucrose synthase
UniProt
Manually annotated by BRENDA team
sucrose synthase 2
UniProt
Manually annotated by BRENDA team
sucrose synthase 3
UniProt
Manually annotated by BRENDA team
quaking aspen
-
-
Manually annotated by BRENDA team
almond
-
-
Manually annotated by BRENDA team
var. nectarina, cv. Zao Hong Yan
-
-
Manually annotated by BRENDA team
var. Rehder var. culta Rehder cv. Hosui, isoforms SS I, SS II
-
-
Manually annotated by BRENDA team
variety N19
-
-
Manually annotated by BRENDA team
var. N19
-
-
Manually annotated by BRENDA team
green alga
-
-
Manually annotated by BRENDA team
isoform Sus3
UniProt
Manually annotated by BRENDA team
isoform Sus4
UniProt
Manually annotated by BRENDA team
Mill, cv. UC82B, two isoforms
-
-
Manually annotated by BRENDA team
Mill., cv. UC82B
-
-
Manually annotated by BRENDA team
L. cv. Desiree
-
-
Manually annotated by BRENDA team
potato, var. Norium no. 1
-
-
Manually annotated by BRENDA team
sus1; gene sus1
UniProt
Manually annotated by BRENDA team
L. Moench
-
-
Manually annotated by BRENDA team
sturt pea
-
-
Manually annotated by BRENDA team
cultivar BY535 with a high starch content and culitvar JM20 with a low starch content
-
-
Manually annotated by BRENDA team
L. cv. Alcedo
-
-
Manually annotated by BRENDA team
wheat, cv. San Augustin INTA
-
-
Manually annotated by BRENDA team
field or faba bean, cv. Maris Bead
-
-
Manually annotated by BRENDA team
var. minor cv. Fribo
-
-
Manually annotated by BRENDA team
mung bean, recombinant enzyme
-
-
Manually annotated by BRENDA team
Populus alba overexpressing a S11E mutant form of Vigna radiata SuSy, the mutation causes a decrease in Km-value for sucrose by a factor of 7
Uniprot
Manually annotated by BRENDA team
hybrid B27xB14
-
-
Manually annotated by BRENDA team
inbred B73 or Pioneer 3183
-
-
Manually annotated by BRENDA team
inbred line B73Ht
-
-
Manually annotated by BRENDA team
maize, isozymes SS1, SS2, sh1/sh1- (Black Mexican Sweet) or sh1bz1-m4-genotype
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
significant and positive association between seed and pod wall dry weight at maturity and peak sucrose synthase activity in seeds of both cultivars and both treatments, irrigation and under a rainout shelter, used to induce terminal drought
metabolism
P49040, Q00917, Q9FHU4
sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis; sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis; sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis
metabolism
Q9SLY2
sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis
metabolism
Q00P15, Q00P16
sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis; sucrose synthase is involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis
metabolism
-
sucrose synthase is a key enzyme regulating the process of rice grain filling meaning conversion of sucrose into starch
metabolism
-
the enzyme is important in starcj biosynthesis in potato tubers, changes in SuSy activity do not affect the expression of genes directly involved in starch metabolism, but might lead to important changes in the tuber metabolome and N -glycome
metabolism
-
sucrose synthase is an integral component of the cellulose synthesis
metabolism
-
SuSy is the key enzyme of sucrose metabolism in fruit
physiological function
-
the enzyme plays an important role in seed filling
physiological function
P49040, Q00917, Q9FHU4
the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses; the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses; the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses
physiological function
Q9SLY2
the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses
physiological function
Q00P15, Q00P16
the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses; the enzyme is involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses
physiological function
-
sucrose synthase is the main sucrose breakdown enzyme in plant sink tissues. Sucrose synthase levels do not limit or regulate carbon transfer in the arbuscular mycorrhizal symbiosis
physiological function
-
the enzyme may play little role in determining sucrose accumulation during muskmelon fruit
physiological function
-
the symbiosis-induced Medicago truncatula sucrose synthase gene MtSucS1 is required for an efficient arbuscular mycorrhiza, overview
physiological function
-
SuSy activation is associated with the rapid accumulation of sucrose in peach fruit. In sink organs, SuSy displays typically the predominant sucrolytic activity
physiological function
-
in sugar-starved cells, the in situ activity of both sucrose synthase and invertase decrease significantly. Following supplementation of root meristems with sugar, invertase remaines inactive, but sucrose synthase activity increases. In sugar-starved cells, sucrose synthase activity is induced more by exogenous sucrose than by glucose. The sucrose-induced activity is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities. Prolongation of regeneration and a marked decrease in the number of cells resuming proliferation and resulting from the action of inhibitors, are correlated with the process of sucrose synthase activation at the beginning of regeneration from sugar starvation
physiological function
P31925
sucrose synthase activity is negatively correlated with sucrose and positively correlated with hexose sugars
physiological function
G1FNX7
cell wall-localized isoform SusC may provide UDP-glucose for cellulose and callose synthesis from extracellular sugars
physiological function
-
enzymic activities in organs of a mutant lacking the activities of isoforms sus1, sus2, sus3, sus4 is about 80-90% of those found in wild-type
physiological function
Q00917
isoform Sus2 knockout mutant shows 30-50% less sucrose synthase activity than wild-type and therefore accumulates 40% more sucrose and 50% less fructose at 15 days after flowering. Mutation does not affect the hexose phosphate pool, but leads to 30-70% less starch in embryo and seed coat. Lipid content is are 55% higher at 9-15 days after flowering. Final seed size and composition are unaltered due to an earlier cessation of growth, thus giving rise to an apparent silent phenotype of mature mutant seeds; isoform Sus3 knockout mutant shows 30-50% less sucrose synthase activity than wild-type and therefore accumulates 40% more sucrose and 50% less fructose at 15 days after flowering. Mutation does not affect the hexose phosphate pool, but leads to 30-70% less starch in embryo and seed coat. Lipid content is are 55% higher at 9-15 days after flowering. Final seed size and composition are unaltered due to an earlier cessation of growth, thus giving rise to an apparent silent phenotype of mature mutant seeds
physiological function
-
mutant lacking isoforms sus1/sus2/sus3/sus4 displays wild-type SUS5 and SUS6 expression levels in leaf, whereas leaves of the sus5/sus6 mutant display wild-type SUS1-4 expression levels. Sucrose synthase activity in leaves and stems of the sus1/sus2/sus3/sus4 and sus5/sus6 plants is about 85% of that of wild-type leaves, which can support normal cellulose and starch biosynthesis; mutant lacking isoforms sus1/sus2/sus3/sus4 displays wild-type SUS5 and SUS6 expression levels in leaf, whereas leaves of the sus5/sus6 mutant display wild-type SUS1-4 expression levels. Sucrose synthase activity in leaves and stems of the sus1/sus2/sus3/sus4 and sus5/sus6 plants is about 85% of that of wild-type leaves, which can support normal cellulose and starch biosynthesis
metabolism
-
starch synthesis
additional information
-
roots of plants deficient in sucrose synthase root isozyme are colonized successfully by arbuscular myccorhizal fungi in a similar levelthan the wild-type plant roots
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
Q7Y078
-
-
-
?
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
Q7Y078
ADP-glucose produced by SuSy is linked to starch biosynthesis. SuSy exerts a strong control on the starch biosynthetic process and controls the production of ADP-glucose accumulating in source leaves
-
-
?
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
-
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
-
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
-
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
-
CDP-glucose + D-fructose
CDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
-
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
GDP-glucose + D-fructose
GDP + sucrose
show the reaction diagram
-
-
-
-
r
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
Q01390
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
-
-
-
-
r
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
-
-
-
-
r
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
-
-
-
-
-
TDP-glucose + D-fructose
TDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
P13708
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
Leleba oldhami
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
P49037
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
Q6SJP5, Q94G60
-
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
poor substrate: GDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
also substrate: ADP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
CDP is poor substrate
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
CDP is poor substrate
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
CDP is poor substrate
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
no substrate: GDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
no substrate: GDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
substrate: TDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
substrate: TDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
substrate: TDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
with SS2-isozyme TDP is 57%, with SS1-isozyme 91% as effective as UDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
substrate: CDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
substrate: CDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
ADP is less effective
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
ADP is less effective
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
ADP is less effective
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
CDP can replace UDP
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
major enzyme of sucrose synthesis
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
involved in sucrose metabolism
-
-
-
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
involved in sucrose metabolism
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
regulation of enzyme
-
-
-
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
regulation of enzyme
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
role of isoforms I, II
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
biochemical pathway for sucrose degradation. Repression of invertase and mobilization of sucrose via the energetically less costly route provided by SuSY is important in growing tubers because it conserves oxygen and allows higher internal oxygen tensions to be maintained
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
under appropriate conditions, the enzyme participates in the in vitro mobilization of sucrose from tonoplast vesicles
-
-
?
UDP-alpha-D-galactose + D-fructose
UDP + sucrose
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-glucose + 1-deoxy-1-fluoro-fructose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 175% of the activity with D-fructose, 100% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P10691
-
-
-
r
UDP-alpha-D-glucose + D-glucose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-lyxose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 150% of the activity with D-fructose, 48% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-mannose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 75% of the activity with D-fructose, 40% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-ribose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 7% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-ribulose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 24% of the activity with D-fructose, no activity with enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-tagatose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 43% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + D-talose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 117% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + D-xylose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 300% of the activity with D-fructose, 14% for the enzyme expressed in Sacchromyces cerevisiae, the recombinant enzyme expressed in Sacchromyces cerevisiae shows 42% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + L-arabinose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 490% of the activity with D-fructose, 36% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-galactose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli
-
-
?
UDP-alpha-D-glucose + L-glucose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 34% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-mannose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 59% of the activity with D-fructose, 8% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-rhamnose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 52% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucose + L-sorbose
?
show the reaction diagram
P10691
the recombinant enzyme expressed in Escherichia coli shows 96% of the activity with D-fructose, 55% for the enzyme expressed in Sacchromyces cerevisiae
-
-
?
UDP-alpha-D-glucuronic acid + D-fructose
?
show the reaction diagram
-
32% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-xylose + D-fructose
?
show the reaction diagram
-
39% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P49037
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P49040, Q00917, Q9FHU4
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P31926
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P13708
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q9XGB7
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q00P15, Q00P16
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
O24301, O81610, Q9AVR8, Q9T0M9
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P31924
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q0E7D4
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q84T18
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
O49845
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
the level of sucrose synthase activity in the developing cotyledons of plant genotypes differing in seed size at 500 to 600 growing degree days is correlated with the final seed size at maturity
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
typical Michaelis-Menten behavior with respect to both UDP-glucose and D-fructose, no substrate inhibition at any UDP-glucose and D-fructose concentration tested
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + D-fructose
UDP + beta-D-fructofuranosyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside
show the reaction diagram
-
23% of the activity with UDP-alpha-D-glucose
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + D-fructose
UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
show the reaction diagram
-
-
-
-
-
UDP-N-acetyl-alpha-D-glucosamine + D-fructose
UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
show the reaction diagram
-
as active as UDP-alpha-D-glucose
-
-
?
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
P13708
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
P49037
-
-
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
Leleba oldhami
-
sucrose cleavage preferred
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
main substrate is UDPglucose
-
r
UDPglucose + D-fructose
UDP + sucrose
show the reaction diagram
-
main substrate is UDPglucose
-
r
IDP-glucose + D-fructose
IDP + sucrose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
L-sorbose, 5-keto-D-fructose, D-tagatose, fructose 6-phosphate, levanbiose can replace fructose to a small extent, no glucosyl acceptors are D-xylulose, L-rhamnulose, D-glucoheptulose, D-mannoheptulose, turanose, inulobiose, melibiulose, lactulose, cellobiulose, 3,4-di-O-methylfructose, dihydroxyacetone, pyruvate
-
-
-
additional information
?
-
-
it is possible that sucrose synthase provides monosaccharide precursors for mucilage synthesis
-
-
-
additional information
?
-
-
it is supposed that the sucrose synthase activity in sugar-beet roots is controlled by sucrose synthesized in leaves rather than by phytohormones
-
-
-
additional information
?
-
-
mtSUS plays a non-sucrolytic role
-
-
-
additional information
?
-
-
sucrose synthase appears to be largely responsible for feeding assimilated carbon into sink metabolism
-
-
-
additional information
?
-
-
sucrose synthase appears to be largely responsible for feeding assimilated carbon into sink metabolism. SH1 isozyme preferentially provides the substrate for cellulose biosynthesis, whereas SUS1 generates precursors for starch biosynthesis
-
-
-
additional information
?
-
-
SUS2 may function to regulate SUS1 membrane association by formation of SUS1SUS2 hetero-oligomers
-
-
-
additional information
?
-
-
nitrogen fixation can occur in Lotus japonicus nodules even in the absence of LjSUS3 (the major nodule-induced isoform of SUS), so LjSUS1 must also contribute to the maintenance of nitrogen assimilation
-
-
-
additional information
?
-
-
results support the model that nodule-enhanced Suc synthase 1 of the model legume Medicago truncatula is required for the establishment and maintenance of an efficient N-fixing symbiosis
-
-
-
additional information
?
-
-
SuS is involved in the Suc to polysaccharides conversion. Glycogen content in an Anabaena sp. mutant strain with an insertion inactivation of susA was lower than in the wild type strain under diazotrophic conditions, while both glycogen and polysaccharides levels are higher in a mutant strain constitutively overexpressing susA
-
-
-
additional information
?
-
-
sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure, overview
-
-
-
additional information
?
-
P10691
substrate specificities of recombinant wild-type and mutant enzymes expressed in Escherichia coli or in Saccharomyces cerevisia, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
-
sucrose synthase catalyzes the de novo production of ADPglucose linked to starch biosynthesis in heterotrophic tissues of plants
-
-
r
ADP + sucrose
ADP-glucose + D-fructose
show the reaction diagram
Q7Y078
ADP-glucose produced by SuSy is linked to starch biosynthesis. SuSy exerts a strong control on the starch biosynthetic process and controls the production of ADP-glucose accumulating in source leaves
-
-
?
sucrose + UDP
UDP-glucose + D-fructose
show the reaction diagram
Q01390
sucrose loaded into the phloem of a poplar leaf is used directly by sucrose synthase associated with various beta-glucan synthases in the stem for UDPglucose formation in the sink tissue, thereby conserving the high energy bond between glucose and fructose
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
-
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
major enzyme of sucrose synthesis
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
involved in sucrose metabolism
-
-
-
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
involved in sucrose metabolism
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
regulation of enzyme
-
-
-
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
regulation of enzyme
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
role of isoforms I, II
-
-
r
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
biochemical pathway for sucrose degradation. Repression of invertase and mobilization of sucrose via the energetically less costly route provided by SuSY is important in growing tubers because it conserves oxygen and allows higher internal oxygen tensions to be maintained
-
-
?
UDP + sucrose
UDP-glucose + D-fructose
show the reaction diagram
-
under appropriate conditions, the enzyme participates in the in vitro mobilization of sucrose from tonoplast vesicles
-
-
?
UDP-alpha-D-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P10691
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
r
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P49037
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P49040, Q00917, Q9FHU4
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P31926
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P13708
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q9XGB7
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q00P15, Q00P16
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
O24301, O81610, Q9AVR8, Q9T0M9
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
P31924
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q0E7D4
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
Q84T18
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
O49845
-
-
-
?
UDP-glucose + D-fructose
UDP + sucrose
show the reaction diagram
-
the level of sucrose synthase activity in the developing cotyledons of plant genotypes differing in seed size at 500 to 600 growing degree days is correlated with the final seed size at maturity
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
sucrose synthase plays an important role in sugar metabolism during sucrose accumulation in the coffee fruit
-
-
?
ADP-glucose + D-fructose
ADP + sucrose
show the reaction diagram
-
-
-
-
r
additional information
?
-
-
it is possible that sucrose synthase provides monosaccharide precursors for mucilage synthesis
-
-
-
additional information
?
-
-
it is supposed that the sucrose synthase activity in sugar-beet roots is controlled by sucrose synthesized in leaves rather than by phytohormones
-
-
-
additional information
?
-
-
mtSUS plays a non-sucrolytic role
-
-
-
additional information
?
-
-
sucrose synthase appears to be largely responsible for feeding assimilated carbon into sink metabolism
-
-
-
additional information
?
-
-
sucrose synthase appears to be largely responsible for feeding assimilated carbon into sink metabolism. SH1 isozyme preferentially provides the substrate for cellulose biosynthesis, whereas SUS1 generates precursors for starch biosynthesis
-
-
-
additional information
?
-
-
SUS2 may function to regulate SUS1 membrane association by formation of SUS1SUS2 hetero-oligomers
-
-
-
additional information
?
-
-
nitrogen fixation can occur in Lotus japonicus nodules even in the absence of LjSUS3 (the major nodule-induced isoform of SUS), so LjSUS1 must also contribute to the maintenance of nitrogen assimilation
-
-
-
additional information
?
-
-
results support the model that nodule-enhanced Suc synthase 1 of the model legume Medicago truncatula is required for the establishment and maintenance of an efficient N-fixing symbiosis
-
-
-
additional information
?
-
-
SuS is involved in the Suc to polysaccharides conversion. Glycogen content in an Anabaena sp. mutant strain with an insertion inactivation of susA was lower than in the wild type strain under diazotrophic conditions, while both glycogen and polysaccharides levels are higher in a mutant strain constitutively overexpressing susA
-
-
-
additional information
?
-
-
sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
enzyme exists as monophosphorylated and multiphosphorylated form, multiphopsphorylation increases affinity for UDP up to threefold
-
additional information
P13708
Ser11 is primary phosphorylation site, phosphorylation does not affect kinetics but partitioning between membrane and cytosol
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
inhibition
Ca2+
-
activation of sucrose synthesis; inhibition
Ca2+
-
activation of sucrose synthesis; slight activation of sucrose cleavage at 10 mM
Ca2+
-
activation of sucrose synthesis; inhibition
Ca2+
-
20 mM, 2.5fold stimulation
Mg2+
-
activation
Mg2+
-
activation
Mg2+
-
activation
Mg2+
-
activation; sucrose synthesis, 0.1-10.0 mM
Mg2+
-
activation; slight activation of sucrose cleavage
Mg2+
-
activation
Mg2+
-
activation; inhibition of sucrose cleavage
Mg2+
-
20 mM, 3fold stimulation
Mg2+
-
activates incoporation of the enzyme into the cellulose synthase particles
Mn2+
-
activation; sucrose synthesis
Mn2+
-
activation; together with Mg2+
Mn2+
-
activation
Mn2+
-
0.1 mM; activation; inhibits at 3 mM
Mn2+
-
activation
Mn2+
-
activation
additional information
-
no cation requirement
additional information
-
no ionic activator requirement
additional information
-
no activation by K+, Na+, NH4+, Cl-, Br-, F-, NO3-, phosphate, sulfate, borate, acetate, citrate
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
-
-
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
P10691
30% inhibition
5-deoxy-beta-D-fructose
-
competitive to D-fructose
5-deoxy-D-fructose
P10691
10% inhibition
6-Dimethylaminopurine
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
Actin
-
0.001 mM, 17% inhibition of SuSy1
-
ADP
-
high concentrations
ADP
-
sucrose cleavage, not synthesis
Ag+
-
at 0.4 mM
AMP
-
not inhibitory
AMP
-
not inhibitory
Arbutin
-
no enzyme activity at 5 mM
ATP
-
strong
ATP
-
sucrose synthesis
ATP
-
inhibition of cleavage, not synthesis
Ca2+
-
sucrose cleavage
Ca2+
-
sucrose cleavage
Co2+
-
0.05 mM, 20% loss of activity
Cu2+
-
at 0.01 mM
Cu2+
-
at 0.4 mM
Cu2+
-
no activity at 1 mM
Cu2+
-
80-90% inhibition at 1 mM
D-fructose
-
mixed inhibitor with regard to both sucrose and UDP, substrate inhibition
D-fructose
-
10 mM, about 80% loss of activity, SuSy1
Fe2+
-
no activity at 1 mM
-
Fe3+
-
0.4 mM
-
fructose
Leleba oldhami
-
competitive to sucrose
fructose
-
high concentration, sucrose synthesis
fructose
-
-
fructose
-
-
fructose
-
kinetics
fructose
-
uncompetitive to UDPglucose
fructose
-
above 20 mM, cosubstrate ADP-Glc, not with UDP-GLC
fructose
-
50% inhibition at 6 mM
fructose
-
noncompetitive
glucose
-
-
glucose
-
uncompetitive to UDPglucose
glucose
-
50% inhibition at 10 mM
glucose
-
uncompetitive
GTP
-
sucrose cleavage
Hg2+
-
at 0.01 mM
Hg2+
-
0.4 mM
Hg2+
-
80% inhibition at 1 mM
imidazole
-
-
iodoacetic acid
-
-
iodoacetic acid
-
partially reversible by GSH or DTT
Mg2+
-
sucrose cleavage
Mg2+
-
sucrose cleavage
Mn2+
-
sucrose cleavage
Mn2+
-
3 mM
N-ethylmaleimide
-
-
N-ethylmaleimide
-
partially reversible by GSH or DTT
okadiac aid
-
sucrose-induced activity of sucrose synthase is strongly inhibited by okadaic acid and less by 6-dimethylaminopurine at early stages of regeneration, but not at the stages preceding DNA replication or mitotic activities
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
partially reversible by GSH or DTT
Salicine
-
kinetics
Sucrose
-
substrate inhibition
Tris
-
sucrose cleavage
Tris
-
ADP + sucrose
UDP
-
strong; sucrose synthesis, partially reversible by MgCl2
UDP
Leleba oldhami
-
strong
UDP
-
competitive to UDPglucose; strong
UDP
-
strong
UDP
-
at high concentrations; strong
UDP
-
kinetics; strong
UDP
-
substrate inhibition
UDP-glucose
-
competitive with respect to UDP and mixed inhibitor with respect to sucrose; substrate inhibition
UDPglucose
-
allosteric inhibition
UDPglucose
Leleba oldhami
-
-
UDPglucose
-
-
UTP
-
synthesis
UTP
-
synthesis
UTP
-
sucrose cleavage; synthesis
Zn2+
-
strong
Zn2+
-
80-90% inhibition at 1 mM
Mn2+
-
sucrose cleavage
additional information
-
not inhibitory: cAMP; not inhibitory: fructose 2,6-diphosphate; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate
-
additional information
-
not inhibitory: DTT, GSH, 2-mercaptoethanol, EDTA; not inhibitory: fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 1,6-diphosphate; not inhibitory: galactose, mannose, maltose, raffinose, 3-phosphoglycerate, phosphoenolpyruvate, ethanol, succinate, 2-oxoglutarate, glutamine, NAD+, diphosphate
-
additional information
-
no inhibition by K+, Na+
-
additional information
-
decrease of enzyme activity in degrading direction in leaves during fruit development
-
additional information
-
typical Michaelis-Menten behavior with respect to both UDP-glucose and D-fructose, no substrate inhibition at any UDP-glucose and D-fructose concentration tested
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
abscisic acid
-
as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
Actin
-
activity of SuSy2 in breakdown direction is stimulated by 60%
-
allantoin
-
activation, sucrose synthesis
Ca2+
-
only for sucrose synthesis
fructose 2,6-bisphosphate
-
stimulates SuSy2 in presence of actin
Mg2+
-
2-4fold activation of sucrose synthesis, not cleavage
Mg2+
-
only for sucrose synthesis
nitrate
-
50% activation at 3-5 mM, exogenous nitrate at 14.2 mM absorbed in the form of KNO3 and Ca(NO3)2 during 10-20 days exponentially activates the enzyme in the roots by 22-100% as compared with plants grown on nitrogen-free medium. Under low light, nitrate can not activate sucrose synthase
-
Sucrose
-
exogenous, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling
UDP-D-xylose
-
activation of epicotyl and cotyledon isozymes
Mn2+
-
2-4fold activation of sucrose synthesis, inhibition of cleavage
additional information
-
peak of activities at the 21th day post anthesis, higher activity in the cultivar with a high starch content
-
additional information
-
increase of enzyme activity in synthezising direction in leaves during fruit development, increase of enzyme activity in synthezising and in degrading direction in mesocarp tissue during fruit development
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.13
-
ADP
-
-
0.15
-
ADP
-
pH 7.0, 37C
0.17
-
ADP
-
pH 7.0, 37C
1.15
-
ADP
-
-
3.3
3.8
ADP
-
-
0.44
0.9
CDP
-
pH 7.2
0.44
0.9
CDP
-
-
3.7
-
CDP
-
-
1.05
-
D-fructose
-
25C, isoform SuSy1
1.1
-
D-fructose
Q6SJP5, Q94G60
pH 7.5, 35C, SuSyI
1.8
-
D-fructose
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyII
2.1
-
D-fructose
-
pH 8.0, 30C
2.65
-
D-fructose
-
25C, isoform SuSy2
5
-
D-fructose
P10691
recombinant enzyme expressed in Saccharomyces cerevisiae
5.5
-
D-fructose
P10691
recombinant enzyme expressed in Escherichia coli
6.49
-
D-fructose
-
pH 7.3
6.49
-
D-fructose
-
pH 7.5, isoform SuSyC
6.62
-
D-fructose
-
pH 7.5, isoform SuSyA
11.7
-
D-fructose
-
pH 7.5, isoform SuSyB
0.012
-
dTDP
-
pH 7.6, 30C
0.98
1.66
fructose
-
cosubstrate UDP
0.98
1.66
fructose
Leleba oldhami
-
-
0.98
1.66
fructose
-
-
0.98
1.66
fructose
-
cosubstrate ADPglucose; cosubstrate CDP
0.98
1.66
fructose
-
cosubstrate UDPglucose, ADP
2.08
3.1
fructose
-
pH 7.2
2.08
3.1
fructose
-
-
2.08
3.1
fructose
-
-
3.7
5
fructose
-
-
3.7
5
fructose
-
-
3.7
5
fructose
-
-
3.7
5
fructose
-
-
6.07
-
fructose
-
-
7.78
-
fructose
-
recombinant enzyme
18.6
-
fructose
-
-
40
-
fructose
-
cosubstrate ADPglucose
0.17
0.2
GDP
-
cosubstrate UDP, ADP, HEPES buffer
0.0916
-
Sucrose
-
pH 7.6, 30C
16.7
-
Sucrose
-
-
16.9
40
Sucrose
Leleba oldhami
-
-
16.9
40
Sucrose
-
cosubstrate ADP
16.9
40
Sucrose
-
-
16.9
40
Sucrose
-
-
16.9
40
Sucrose
-
-
22.3
-
Sucrose
-
25C, isoform SuSy1
30
-
Sucrose
-
cosubstrate: UDP
30.3
-
Sucrose
-
25C, isoform SuSy2
35.9
-
Sucrose
-
pH 7.0
35.9
-
Sucrose
-
pH 7.0, isoform SuSyC
37.6
-
Sucrose
-
-
41.8
-
Sucrose
-
pH 7.0, isoform SuSyA
48
-
Sucrose
-
cosubstrate UDP, pH 7.0, 37C
52
290
Sucrose
-
pH 6
52
290
Sucrose
-
-
52
290
Sucrose
-
-
52
290
Sucrose
-
cosubstrate UDP
52
290
Sucrose
-
-
53
-
Sucrose
-
cosubstrate UDP, pH 7.0, 37C
56
-
Sucrose
-
isoform B
60
-
Sucrose
P10691
recombinant enzyme expressed in Saccharomyces cerevisiae
80.3
-
Sucrose
P10691
recombinant enzyme expressed in Escherichia coli
87
-
Sucrose
-
-
91
-
Sucrose
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyII
100
-
Sucrose
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyI
108
-
Sucrose
-
-
109
-
Sucrose
-
pH 7.0, isoform SuSyB
145
-
Sucrose
-
cosubstrate ADP, pH 7.0, 37C
161
-
Sucrose
-
recombinant enzyme
185
-
Sucrose
-
cosubstrate ADP, pH 7.0, 37C
200
-
Sucrose
-
isoform A
210
-
Sucrose
-
cosubstrate: ADP
288
303
Sucrose
-
-
0.65
-
TDP
-
-
0.00191
-
UDP
-
pH 7.0
0.00191
-
UDP
-
pH 7.0, isoform SuSyC
0.0041
-
UDP
-
pH 7.6, 30C
0.005
-
UDP
-
-
0.0076
-
UDP
-
25C, isoform SuSy1
0.032
-
UDP
-
25C, isoform SuSy2
0.061
0.094
UDP
-
cosubstrate TDP, pH 6
0.061
0.094
UDP
-
-
0.069
-
UDP
-
-
0.14
0.15
UDP
-
Tris buffer
0.14
0.15
UDP
-
-
0.2
-
UDP
P10691
recombinant enzyme expressed in Escherichia coli
0.214
-
UDP
-
pH 7.0, isoform SuSyB
0.25
-
UDP
-
pH 7.0, 37C
0.3
-
UDP
P10691
recombinant enzyme expressed in Saccharomyces cerevisiae
0.31
-
UDP
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyII
0.34
-
UDP
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyI
0.39
-
UDP
-
-
0.39
-
UDP
-
pH 7.0, 37C
0.41
-
UDP
-
-
1.07
-
UDP
-
pH 7.0, isoform SuSyA
1.25
-
UDP
-
-
0.6
-
UDP-alpha-D-glucose
P10691
recombinant enzyme expressed in Saccharomyces cerevisiae
1.4
-
UDP-alpha-D-glucose
P10691
recombinant enzyme expressed in Escherichia coli
0.77
-
UDP-GlcNAc
-
pH 8.0, 30C
0.05
-
UDP-glucose
-
-
0.05
-
UDP-glucose
-
pH 7.6, temperature not specified in the publication
0.234
-
UDP-glucose
-
pH 7.3
0.234
-
UDP-glucose
-
pH 7.5, isoform SuSyC
0.4
-
UDP-glucose
-
recombinant enzyme
0.42
-
UDP-glucose
-
25C, isoform SuSy1
0.43
-
UDP-glucose
Q6SJP5, Q94G60
pH 6.5, 35C, SuSyII
0.45
-
UDP-glucose
-
25C, isoform SuSy2
0.5
-
UDP-glucose
-
pH 8.0, 30C
0.53
-
UDP-glucose
-
pH 7.5, isoform SuSyB
0.6
-
UDP-glucose
Q6SJP5, Q94G60
pH 7.5, 35C, SuSyI
0.83
-
UDP-glucose
-
-
3.59
-
UDP-glucose
-
pH 7.5, isoform SuSyA
25
-
UDP-glucose
-
pH 7.6, temperature not specified in the publication
11.5
-
UDPfructose
-
isozyme SS1
0.012
0.033
UDPglucose
-
-
0.012
0.033
UDPglucose
-
-
0.11
-
UDPglucose
-
-
0.3
0.38
UDPglucose
-
pH 7.2
0.3
0.38
UDPglucose
-
cosubstrate TDP
0.3
0.38
UDPglucose
-
isozyme SS2
1.92
2.7
UDPglucose
Leleba oldhami
-
-
1.92
2.7
UDPglucose
-
-
5.3
6
UDPglucose
-
cosubstrate fructose
5.3
6
UDPglucose
-
-
5.3
6
UDPglucose
-
cosubstrate UDPfructose, isozyme SS2
4.5
-
GDP
-
-
additional information
-
additional information
Leleba oldhami
-
kinetic study
-
additional information
-
additional information
-
kinetic study
-
additional information
-
additional information
-
kinetic study
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.97
-
Sucrose
-
SuSy1, 25C
39.5
-
Sucrose
-
SuSy2, 25C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.3
-
5-deoxy-beta-D-fructose
-
-
0.015
-
Cu2+
-
-
1.8
-
D-fructose
-
pH 7.0, with respect to sucrose, mixed
4.1
-
D-fructose
-
pH 7.0, with respect to UDP, mixed
35.9
-
D-fructose
-
-
2.23
-
fructose
-
substrate inhibition
2.48
-
fructose
-
-
17.2
-
fructose
-
-
4.3
-
glucose
-
-
43
-
glucose
-
uncompetitive to fructose
53
-
glucose
-
uncompetitive to UDP-glucose
1
1.5
Mn2+
-
sucrose cleavage
0.035
-
Ni2+
-
-
227
-
Sucrose
-
substrate inhibition
0.086
-
UDP
-
substrate inhibition
0.104
-
UDP-glucose
-
substrate inhibition
0.12
-
UDP-glucose
-
pH 7.0, with respect to UDP, competitive
0.18
-
UDP-glucose
-
pH 7.0, with respect to sucrose, mixed
2.3
-
UDP-glucose
-
-
0.025
-
Zn2+
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.5
-
2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
P10691
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.003
-
-
value about, degrading direction, leaf, 20 days after anthesis; value about, synthezising direction, leaf, 2 days before anthesis
0.004167
-
-
value about, synthezising direction, leaf, 8 days after anthesis
0.005
-
-
value about, degrading direction, leaf, 16 days after anthesis; value about, synthezising direction, leaf, 16 days after anthesis
0.0067
-
-
value about, degrading direction, leaf, 12 days after anthesis; value about, synthezising direction, leaf, 20 days after anthesis; value about, synthezising direction, mesocarp tissue, 16 days after anthesis
0.0083
-
-
value about, degrading direction, leaf, 4 days and 8 days after anthesis; value about, synthezising direction, mesocarp tissue, 20 days after anthesis; value about, synthezising direction, mesocarp tissue, 2 days before anthesis
0.01
-
P10691
crude extract, recombinant enzyme in Escherichia coli
0.01
-
-
value about, degrading direction, leaf, day of anthesis; value about, synthezising direction, mesocarp tissue, 12 days after anthesis
0.0125
-
-
value about, degrading direction, leaf, 2 days before anthesis; value about, synthezising direction, mesocarp tissue, 8 days after anthesis
0.013
-
-
value about, synthezising direction, mesocarp tissue, day of anthesis
0.1
-
-
value about, degrading direction, mesocarp tissue, 2 days before anthesis and day of anthesis
0.1167
-
-
value about, degrading direction, mesocarp tissue, 4 days after anthesis
0.183
-
-
value about, degrading direction, mesocarp tissue, 8 days after anthesis
0.2083
-
-
value about, synthezising direction, mesocarp tissue, 12 to 20 days after anthesis
0.3
-
P10691
crude extract, recombinant enzyme in Saccharomyces cerevisiae
0.33
-
-
SUS activity in rice grain untreated during grain filling
0.45
-
-
SUS activity in rice grain treated with abscisic acid during grain filling
0.53
-
-
SUS activity in rice grain treated with sducrose and abscisic acid during grain filling
0.66
-
-
SUS activity in rice grain treated with sucrose during grain filling
0.89
-
-
-
0.91
-
-
-
3.27
-
-
-
3.54
-
-
-
3.85
-
-
-
4.35
-
-
-
4.5
-
P10691
purified recombinant enzyme, expressed in Escherichia coli
6
-
-
isozyme SS2
6.8
-
-
wild-type line ICCV 96029
6.9
-
P10691
purified recombinant enzyme, expressed in Saccharomyces cerevisiae
8.34
-
-
-
9.5
-
-
wild-type line 96030
10
-
-
isozyme SS1
15.1
-
-
-
128
-
-
sucrose cleavage
136.1
-
Q6SJP5, Q94G60
-
180
-
-
sucrose synthesis
additional information
-
-
-
additional information
-
-
-
additional information
-
-
enzyme activity during seed development, overview
additional information
-
-
the level of sucrose synthase activity in the developing cotyledons of plant genotypes differing in seed sizeat 500 to 600 growing degree days is correlated with the final seed size at maturity
additional information
-
-
specific activities in engineered and wild-type lines, the first show higher activities, in nmol/min x g FW
additional information
-
-
SuSy activity: 90 mU/g FW in wild-type tubers, 170-250 mU/g FW in transgenic overexpressing tubers
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
-
-
assay at
6
6.5
-
sucrose cleavage
6
-
-
sucrose cleavage
6
-
-
sucrose cleavage
6
-
-
sucrose cleavage
6
-
-
sucrose cleavage
6
-
Q6SJP5, Q94G60
cleavage of sucrose, SuSyI
6.4
-
-
sucrose cleavage
6.5
7
-
in 0.2 M HEPES-NaOH, varies with buffer
6.5
-
-
sucrose cleavage
6.5
-
-
sucrose cleavage
6.5
-
-
sucrose cleavage
6.5
-
Q6SJP5, Q94G60
synthesis of sucrose, SuSyII
6.6
-
-
calculated from sequence, enzyme form CaSUS2
7
9.5
-
broad, sucrose synthesis
7
-
-
sucrose synthesis
7
-
-
sucrose cleavage
7
-
-
sucrose cleavage
7
-
Q6SJP5, Q94G60
synthesis of sucrose, SuSyII
7
-
-
recombinant isoforms SUS1 and SUS3; recombinant isoforms SUS1 and SUS3
7.5
-
-
sucrose synthesis, isozyme SS2
7.5
-
Q6SJP5, Q94G60
synthesis of sucrose, SuSyI
7.5
-
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
assay at; assay at; assay at; assay at; assay at; assay at; assay at
7.5
-
-
assay at
7.5
-
-
assay at
7.6
-
-
cleavage of sucrose
8
-
-
sucrose synthesis
8
-
-
sucrose synthesis
8
-
-
synthesis of sucrose
8
-
-
assay at
8.5
-
-
sucrose synthesis
8.5
-
-
assay at
9
-
-
sucrose synthesis
9
-
-
isozyme SS; sucrose synthesis
9.5
-
-
sucrose synthesis
additional information
-
-
pI: 6.16
additional information
-
-
pI: 5.8, isozyme SS1
additional information
-
-
pI: 5.4,isozyme SS2
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
10
-
about half-maximal activity at pH 5.0 and 10.0, sucrose synthesis
5
8
-
about half-maximal activity at pH 5.0 and 8.0, sucrose cleavage
5
8.2
-
about 90% of maximal activity at pH 5.0 and about half-maximal activity at pH 8.2, sucrose cleavage
5.5
9
-
about 80% of maximal activity at pH 5.5 and about half-maximal activity at pH 9.0, sucrose cleavage
5.6
10
-
about half-maximal activity at pH 5.6 and about 90% of maximal activity at pH 10.0, sucrose synthesis
6.2
6.6
-
96% of maximal activity at pH 6.2 and 6.6
7.5
10
-
about half-maximal activity at pH 7.5 and about 90% of maximal activity at pH 10.0, sucrose synthesis
9.5
-
-
about 10-15% of maximum activity; about 10-15% of maximum activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
30
-
-
assay at
30
-
-
assay at
30
-
-
assay at
37
-
-
assay at
45
50
Q6SJP5, Q94G60
pH 6.5, cleavage of sucrose, SuSyII
45
-
Q6SJP5, Q94G60
pH 7.5, synthesis of sucrose, SuSyI
50
60
-
in 0.2 M Hepes-NaOH, varies with buffer
50
-
Q6SJP5, Q94G60
pH 6.5, cleavage of sucrose, SuSyI. Synthesis of sucrose, SuSy II
55
-
-
-
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.9
-
C6H0M2, F8SM21, -, P31922
calculated
6.3
-
C6H0M2, F8SM21, -, P31922
calculated
6.7
-
-
calculated from sequence, enzyme form CaSUS1
8.6
-
C6H0M2, F8SM21, -, P31922
calculated
additional information
-
-
total protein extract shows two cross-reacting spots at 88000 Da with a pI of 5.5 to 6.0
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
CaSUS1 expression is barely detectable in young flower buds and leaves. Transcripts of this isoform are observed in old flower buds and roots, and accumulates to a high level in stems
Manually annotated by BRENDA team
-
developing, sucrose synthase reaches a maximum about 500 to 600 growing degree days after flowering, when cell numbers were no longer increasing, but the cotyledons are increasing rapidly in dry weight
Manually annotated by BRENDA team
-
at least one form of SuSy present in young tissue is absent, or present below detection limits, in mature culm tissue
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
mRNA levels of CaSUS2 is undetectable in young endosperm, but increased towards the ripening of endosperm tissues
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
G1FNX7
expressed at high levels during secondary cell wall synthesis in fiber
Manually annotated by BRENDA team
-
CaSUS1 expression is barely detectable in young flower buds and leaves. Transcripts of this isoform are observed in old flower buds and roots, and accumulates to a high level in stems; CaSUS2 expression is low in flower buds and in flowers whether young or mature
Manually annotated by BRENDA team
-
CaSUS1 mRNAs accumulats mainly during the early development of perisperm and endosperm, as well as during pericarp growing phases; CaSUS2 transcript levels is maximal at later stages of pericarp (205234 day after flowering) and endosperm (234 day after flowering) development. Weak CaSUS2 expression is observed in the early stages (60 and 89 day after flowering) of perisperm development, as well as at 176 day after flowering
Manually annotated by BRENDA team
-
very low enzyme activity in young and unripe muskmelons, but rapid accumulation of sucrose during ripening
Manually annotated by BRENDA team
-
SuSy activity increases dramatically during fruit maturation, while activity is low at the young fruit developmental stage, still lower at the endocarp hardening stage
Manually annotated by BRENDA team
E0Z1D0, E0Z1D1
in green fruit, isoform Sus4 is expressed at nearly 200fold lower levels than isoform Sus3. Elevated level of expression in ripening fruit for isoform Sus3; in green fruit, isoform Sus4 is expressed at nearly 200fold lower levels than sioform Sus3
Manually annotated by BRENDA team
-
level can differ significantly in different parts of the internodes and with maturity. Both vascular and storage parenchyma tissue contain SuSy in young and mature internodes
Manually annotated by BRENDA team
-
SUS1 is predominant in the internode cortex tissue
Manually annotated by BRENDA team
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
SUS1
Manually annotated by BRENDA team
P31925
enzymic activity is higher in immature internodes than in mature internodes in all cultivars, sucrose synthase transcript expression shows a similar pattern. Sucrose synthase activity is negatively correlated with sucrose and positively correlated with hexose sugars
Manually annotated by BRENDA team
-
SUS1 is the predominant isoform of SUS associated with microsomes isolated from the base of the maize leaf elongation zone and from kernels at 20 and 30 days after pollination. SUS2 exists predominantly as a hetero-oligomer with SUS1 in kernels; SUS2 is particularly abundant in kernels at various pollination stages; SUSSH1 is predominant in developing kernels
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
-
high expression of Msus1 mRNA in expanding leaves, not detectable in expanding leaves
Manually annotated by BRENDA team
-
high CaSUS2 gene expression is observed in light-exposed leaves (positioned at the terminal ending of the branch). CaSUS2 mRNA levels is moderate in shaded leaves (collected inside the plant)
Manually annotated by BRENDA team
-
low level of expression
Manually annotated by BRENDA team
-
elongation zone, isoenzyme SUS2. SUS2 exists predominantly as a hetero-oligomer with SUS1 in kernels; elongation zone, isoenzyme SUS-SH1; SUS1 is the predominant isoform of SUS associated with microsomes isolated from the base of the maize leaf elongation zone and from kernels at 20 and 30 days after pollination
Manually annotated by BRENDA team
-
light and metabolic signals control the selective degradation of sucrose synthase in maize leaves during deetiolation. SUS degradation is important to supply residues for the synthesis of other proteins required for autotrophic metabolism
Manually annotated by BRENDA team
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
; developing; developing, SUS1
Manually annotated by BRENDA team
-
2 genotypes ICPL 84023 and ICP 301 tolerant to waterlogging stress, and 2 genotypes ICP 7035 and Pusa 207 susceptible to waterlogging stress. Pattern of variation in reducing sugar content in the 4 genotypes is parallel to sucrose synthase activity. ICPL 84023 and ICP 301 also show fewer declines in total and non-reducing sugars and greater increase in reducing sugar and SuSy activity than ICP 7035 and Pusa 207
Manually annotated by BRENDA team
-
nodulaid inoculum: Rhizobium japonicum CB 1809
Manually annotated by BRENDA team
-
Bradyrhizobium japonicum strains
Manually annotated by BRENDA team
-
; mRNA levels of CaSUS2 is barely detectable in young pericarp, but increased towards the ripening of pericarp tissues
Manually annotated by BRENDA team
-
mRNA levels of CaSUS2 is barely detectable in young pericarp
Manually annotated by BRENDA team
-
SuSy is specifically localized in the creous walled sieve element of the phloem
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus
Manually annotated by BRENDA team
-
sucrose synthase has a role in the construction of the extracellular matrix and thus in the morphogenesis of pollen tubes. Distribution of Sus is affected by brefeldin A and depended on the nutrition status of the pollen tube, because an absence of metabolic sugars in the growth medium caused Sus to distribute differently during tube elongation
Manually annotated by BRENDA team
-
increased activity is in the tip region and in the stele of root axes. Increased in situ activity correlates with cell wall thickening by cellulose deposition under hypoxia
Manually annotated by BRENDA team
-
it is supposed that the sucrose synthase activity in sugar-beet roots is controlled by sucrose synthesized in leaves rather than by phytohormones
Manually annotated by BRENDA team
-
CaSUS1 expression is barely detectable in young flower buds and leaves. Transcripts of this isoform are observed in old flower buds and roots, and accumulates to a high level in stems; high CaSUS2 gene expression is observed in roots
Manually annotated by BRENDA team
-
high level of expression
Manually annotated by BRENDA team
-
SUS1 is predominant in etiolated shoots
Manually annotated by BRENDA team
-
activities of sucrose synthase is not significantly higher in wounded roots than in unwounded controls at any time during 13 days of postwounding storage
Manually annotated by BRENDA team
-
SBSS1 transcript levels are elevated in wounded, anoxic and cold-treated roots. SBSS1 protein levels exhibit little change in stressed roots, even after 7 d. SBSS1 expression in response to wounding, anoxia and cold may be regulated by post-transcriptional mechanisms; SBSS2 transcript levels are elevated in wounded, anoxic and cold-treated roots. SBSS2 protein levels exhibit little change in stressed roots, even after 7 d. SBSS2 expression in response to wounding, anoxia and cold may be regulated by post-transcriptional mechanisms
Manually annotated by BRENDA team
-
an 80% to 90% reduction of MtSucS1 proteins in roots and root nodules specifically limit plant growth and organ development under nodulation-dependent conditions, leading to marked changes in expression of genes involved in the nodule C and N metabolism and consequently to a reduced performance of amino acid biosynthesis
Manually annotated by BRENDA team
P49040, Q00917, Q94CC8, Q9FHU4, Q9FX32, Q9M111
-
Manually annotated by BRENDA team
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
SUS1
Manually annotated by BRENDA team
-
enzyme is localized to root cap
Manually annotated by BRENDA team
E0Z1D0, E0Z1D1
isoform sus4 is expressed at nearly 200fold lower levels than sioform Sus3; isoform sus4 is expressed at nearly 200fold lower levels than sioform Sus3
Manually annotated by BRENDA team
-
preferentially localized in the endopolyploid outer cells
Manually annotated by BRENDA team
-
sucrose synthase is one of the central enzymes in sucrose cleavage in root nodules; sucrose synthase is one of the central enzymes in sucrose cleavage in root nodules. SuSy2 is the second most abundant isoform present in Medicago truncatula root nodules
Manually annotated by BRENDA team
O24301
transcriptional and post-translational regulation of sucrose synthase in pea nodules by the cellular redox state
Manually annotated by BRENDA team
-
an 80% to 90% reduction of MtSucS1 proteins in roots and root nodules specifically limit plant growth and organ development under nodulation-dependent conditions, leading to marked changes in expression of genes involved in the nodule C and N metabolism and consequently to a reduced performance of amino acid biosynthesis
Manually annotated by BRENDA team
Arachnis hookeriana x Ascocenda Madame Kenny
-
high expression of Msus1 mRNA
Manually annotated by BRENDA team
-
ripening
Manually annotated by BRENDA team
-
developing endosperm
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 day after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus. Co-localization of SUS protein and starch grains in the seed coat at 3 and 10 day after flowering indicates that SUS may be involved in temporary starch deposition during the early stages of seed development
Manually annotated by BRENDA team
-
in endosperm, not in the embryo, immunohistochemic analysis
Manually annotated by BRENDA team
-
cotyledon, epicotyl and root of etiolated seedling
Manually annotated by BRENDA team
P49040, Q00917, Q94CC8, Q9FHU4, Q9FX32, Q9M111
-
Manually annotated by BRENDA team
Leleba oldhami
-
-
Manually annotated by BRENDA team
-
sucrose synthase activity is increased in shoot tips of the transgenic apple plants with decreased sorbitol synthesis compared with the untransformed control
Manually annotated by BRENDA team
-
SUS1 is predominant in etiolated shoots
Manually annotated by BRENDA team
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
-
Manually annotated by BRENDA team
-
at 3 d and 10 d after flowering, SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 d after flowering, SUS protein is detected in the embryo and aleurone layer, but is absent from the seed coat and funiculus. Within the silique wall, SUS localizes specifically to the companion cells, indicating that SUS activity may be required to provide energy for phloem transport activities in the silique wall
Manually annotated by BRENDA team
-
CaSUS1 expression is barely detectable in young flower buds and leaves. Transcripts of this isoform are observed in old flower buds and roots, and accumulates to a high level in stems; CaSUS2 mRNA levels is moderate in stems
Manually annotated by BRENDA team
E0Z1D0, E0Z1D1
elevated expression of isoform Sus4 in the higher, less mature portions of stem; isoform SUS3 expression is not significantly altered throughout the internodes
Manually annotated by BRENDA team
Gladiolus sp.
-
-
Manually annotated by BRENDA team
additional information
Arachnis hookeriana x Ascocenda Madame Kenny
-
expression of Msus1 mRNA is not detectable in flowers
Manually annotated by BRENDA team
additional information
-
transcript abundance of SBSS2, relative to SBSS1, is greater in young vegetative and floral tissues, and reduced in mature vegetative tissues
Manually annotated by BRENDA team
additional information
-
sucrose concentration modulates the ability of SUS1 to associate with F-actin in vitro
Manually annotated by BRENDA team
additional information
-
differential tissue expression patterns
Manually annotated by BRENDA team
additional information
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
tissue-specific expression analysis; tissue-specific expression analysis; tissue-specific expression analysis, SUS2 is widely expressed in tissues; tissue-specific expression analysis, SUS3 is predominantly expressed in the caryopsis; tissue-specific expression analysis, SUS4 is predominantly expressed in the caryopsis; tissue-specific expression analysis, SUS5 is widely expreesed in tissues at low level and is suppressed in germinating shoots under submergence; tissue-specific expression analysis, SUS6 is widely expressed in tissues at low levels and is suppressed in germinating shoots under submergence
Manually annotated by BRENDA team
additional information
C6H0M2, F8SM21, -, P31922
faint expression in all tissues tested
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
C6H0M2, F8SM21, -, P31922
inner side of cell membrane; inner side of cell membrane
-
Manually annotated by BRENDA team
-
analysis by bidimensional electrophoresis indicates that Sus exists as two isoforms, one of which is phosphorylated and more abundant in the cytoplasm and cell wall and the other of which is not phosphorylated and is specific to the plasma membrane
Manually annotated by BRENDA team
C6H0M2, F8SM21, -, P31922
within the cytoplasm and along cytoplasmic tracks; within the cytoplasm and along cytoplasmic tracks
Manually annotated by BRENDA team
-
SUS2 may be the housekeeping SUS isoform because it is solely cytosolic and therefore cleaves sucrose for metabolic requirements within the cytosol
Manually annotated by BRENDA team
-
SUS1 is associated with membranes; SUS-SH1 is associated with membranes
Manually annotated by BRENDA team
-
partial membrane localization when expressed in Escherichia coli. Two regions of SUS1 contribute to membrane affinity: the amino-terminal noncatalytic domain, and a region with sequence similarity to the C-terminal pleckstrin homology domain of human pleckstrin. Alanine substitutions within the pleckstrin homology-like domain of SUS1 reduce membrane association in Escherichia coli and with plant microsomes in vitro without reducing enzymatic activity
Manually annotated by BRENDA team
-
a mechanism involving protein phosphorylation/dephosphorylation and/or calcium would in part control the association of the isoforms of sucrose synthase with membranes in developing fruit
Manually annotated by BRENDA team
-
there are soluble and membrane-bound forms of SuS in Anabaena
Manually annotated by BRENDA team
Q01390
membrane
-
Manually annotated by BRENDA team
-
SUS-SH1 is associated with microsomes
-
Manually annotated by BRENDA team
-
at about 12 h of sucrose provision
Manually annotated by BRENDA team
-
analysis by bidimensional electrophoresis indicates that Sus exists as two isoforms, one of which is phosphorylated and more abundant in the cytoplasm and cell wall and the other of which is not phosphorylated and is specific to the plasma membrane
Manually annotated by BRENDA team
-
the plasma membrane-associated rosette anchors the catalytic unit of cellulose synthesis to form the functional cellulose synthesis machinery
Manually annotated by BRENDA team
-
of pollen tube, colocalization with callose synthase and cellulose synthase. Sucrose synthase binds to actin filaments depending on sucrose concentration. Its distribution within the plasma membrane is dependent on the actin cytoskeleton and the endomembrane system but not on microtubules
Manually annotated by BRENDA team
-
at about 3 h of sucrose provision
Manually annotated by BRENDA team
-
there are soluble and membrane-bound forms of SuS in Anabaena
-
Manually annotated by BRENDA team
-
tonoplast enzyme is approximately 7% of the total tissue activity
-
Manually annotated by BRENDA team
C6H0M2, F8SM21, -, P31922
associated with mitochondrion
Manually annotated by BRENDA team
additional information
-
native SUS2 is not associated with membranes in vivo
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
82000
-
-
calculated from sequence, enzyme form CaSUS2
92500
-
-
calculated from sequence, enzyme form CaSUS1
320000
-
-
gel filtration
320000
-
Q6SJP5, Q94G60
SuSyI and SuSyII, gel filtration
350000
-
-
gel filtration, recombinant enzyme
353000
-
-
gel filtration
360000
-
-
gel filtration
360000
-
-
analytical ultracentrifugation in the presence of Mg2+
360000
-
-
gel filtration, form SS-II
360000
-
-
gel filtration, SS I and SS II
362000
-
-
gel filtration
370000
-
-
germ, gel filtration
375000
-
-
low speed sedimentation equilibrium centrifugation
380000
-
-
leaf, gel filtration
380000
-
-
SDS-PAGE
394000
-
-
gel filtration
405000
-
-
gel filtration
410000
-
-
gel filtration
410000
-
-
SDS-PAGE
412000
-
-
gel filtration
420000
-
-
gel filtration
420000
-
-
gel filtration
440000
-
-
gel filtration
440000
-
-
gel filtration, all 4 isozymes
440000
-
-
-
540000
-
-
gel filtration
540000
-
-
gel filtration
additional information
-
-
amino acid composition
additional information
-
-
amino acid composition
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 88000, SDS-PAGE
?
G1FNX7
x * 90000, SDS-PAGE
oligomer
-
x * 35000 + x * 70000, SDS-PAGE
oligomer
-
x * 87000, SDS-PAGE
oligomer
-
SUS-SH1 forms only homooligomers
tetramer
-
4 * 87000, SDS-PAGE
tetramer
-
4 * 110000, SDS-PAGE
tetramer
-
4 * 92000, SDS-PAGE
tetramer
-
4 * 100000, SDS-PAGE
tetramer
-
4 * 94000, SDS-PAGE
tetramer
-
4 * 88000, SDS-PAGE, depending on the ionic species and ionic strength of the solution, the enzyme can aquire catalytically active, tetrameric, octameric and other higher aggregated forms of which the tetramer is the predominant form
tetramer
-
4 * 90000, SDS-PAGE
tetramer
-
4 * 92600, SDS-PAGE
tetramer
-
4 x 92000, SDS-PAGE, form SS-II, N-terminal sequence
tetramer
-
4 x 83000, SDS-PAGE, SS I and SS II
tetramer
-
4 x 95000, SDS-PAGE, recombinant enzyme
tetramer
-
4 x 80000, SDS-PAGE
tetramer
-
4 x 94000, SDS-PAGE, all 4 isozymes
tetramer
-
4 * 90300, SDS-PAGE
tetramer
-
4 * 94000, SuSyA, SuSyB or SuSyC, SDS-PAGE
tetramer
Q6SJP5, Q94G60
2 * 84000 + 2 * 86000, SuSyI, SDS-PAGE; 4 * 86000, SuSyII, SDS-PAGE
tetramer
P49040, Q00917, Q9FHU4
-
tetramer
Q9SLY2
-
tetramer
-
-
tetramer
Q0E7D4
-
tetramer
O49845
-
tetramer
Q00P15, Q00P16
;
tetramer
P13708
-
tetramer
Q9XGB7
-
tetramer
P31924
-
tetramer
O24301, O81610, Q9AVR8, Q9T0M9
-
tetramer
P49037
-
tetramer
Q84T18
-
tetramer
-
-
?
C6H0M2, F8SM21, -, P31922
x * 92200, calculated; x * 92300, calculated; x * 98700, calculated
additional information
P49037
comparison of structure and active site of starch, glycogen, sucrose synthases
additional information
-
SUS2 exists predominantly as a heterooligomer with SUS1; SUS2 predominantly exists as a hetero-oligomer with SUS1
additional information
-
SUS1 has a unique 28 residue coiled-coil domain that does not appear to play a role in oligomerization
additional information
-
the enzyme forms the catalytic unit of the cellulose synthesis machinery as detergent-soluble granular particles enriched with a 78 kDa polypeptide of sucrose synthase, identification by mass spectrometry and immunoblotting. The catalytic units bind to plasma membrane-associated rosette structures and retain the cellulose synthesis activity in the presence of UDP-glucose or sucrose plus UDP when supplemented with magnesium, incorporation of the catalytic unit into the rosette structure, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
-
nodule SuSy1 is hyperphosphorylated at Ser11
phosphoprotein
-
analysis by bidimensional electrophoresis indicates that Sus exists as two isoforms, one of which is phosphorylated and more abundant in the cytoplasm and cell wall and the other of which is not phosphorylated and is specific to the plasma membrane
phosphoprotein
-
11 phosphorylation sites. The enzyme is largely dephosphorylated in fruits fed EDTA or staurosporine, compared to fruits fed water or sucrose
phosphoprotein
P10691
the enzyme is phosphorylated at Ser11
phosphoprotein
-
S170 phosphorylation promotes the formation of high molecular mass forms, ubiquitin/sucrose-synthase conjugates than can be targeted for 26S-proteasome-dependent degradation
phosphoprotein
-
phosphorylated at a C-terminal threonine residue only in midvein; phosphorylated at a C-terminal threonine residue only in midvein. SUS-SH1 is phosphorylated in vivo at the Ser10 site in kernels
phosphoprotein
-
calcium-dependent protein kinase-mediated phosphorylation of recombinant SUS1 at the Ser15 site is a negative regulator of its association with actin
phosphoprotein
-
SUS is phosphorylated at Ser15 and Ser170 in deetiolation
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
as a complex with UDP-glucose and as a complex with UDP and fructose, at 2.8- and 2.85 A resolution, respectively
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
-
below, complete inactivation
5.5
8
-
stable
6.5
-
-
t1/2: 5 h, 37C
6.5
-
-
t1/2: 1 min, 55C, complete inactivation within 10 min
7.5
-
-
-20C, 6 months, 10% loss of activity
8
-
-
most stable
8
-
-
above, gradual loss of activity
8
-
-
55C: 20% loss of activity within 1 min, 80% loss of activity within 10 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
pH 7.6, 200 mM HEPES-NaOH buffer, 48 h, 20% loss of activity
37
-
-
t1/2: 5 h, pH 6.5
55
-
-
pH 6.5: t1/2: 1 min, complete inactivation after 10 min, pH 8: 20% loss of activity after 1 min, 80% loss of activity within 10 min
60
-
-
and above, 5 min, rapid loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-mercaptoethanol stabilizes
-
EDTA, 0.1 mM, stabilizes
-
freeze-thawing inactivates
-
high salt concentrations inactivate
-
protamin sulfate stabilizes
-
2-mercaptoethanol stabilizes
-
no activity is lost during 3 freeze-thaw-cycles
-
DTT, 0.1 mM, stabilizes
-
EDTA, 0.1 mM, stabilizes
-
freeze-thawing inactivates
-
repeated freeze-thawing leads to slight decrease of activity, even in the presence of PMSF
-
repeated freeze-thawing, stable to if foaming is avoided
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
marked inactivation upon freezing and thawing
-
4C, isozyme SS1, 4 months, isozyme SS2, t1/2: 3 weeks
-
4C, 20 mM potassium phosphate buffer, pH 7.0, 5 mM 2-mercaptoethanol, 4 weeks stable
-
-20C, 20% loss of activity within 2 weeks, t1/2: 1 month
-
-20C, pH 7.5, stored for at least 6 months with 10% loss of activity
-
4C, 0.1 mM EDTA and DTT, more than 50% of activity retained after 1 month
-
-20C, 50% glycerol, stable
-
-20C, about 75% of activity retained after 4 months
-
-80C, 20% glycerol, 0.1-1.0 mM DTT, stable
-
4C, prolonged storage leads to slight decrease of activity, even in the presence of PMSF
-
4C, storage leads to gradual precipitation of denatured protein
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant
Arachnis hookeriana x Ascocenda Madame Kenny
-
native enzyme partially by cation exchange chromatography
-
2 isozymes: SS1 and SS2, partial
-
partial, 2 isozymes
-
isoform SuSy1 and SuSy2
-
3 isozymes, heterotetramers of ss1, SS2 subunits
-
no isozymes detected by isoelectric focusing
-
at least 3 isozymes
-
native enzyme partially by gel filtration
-
native enzyme partially by gel filtration, ammonium sulfate fractionation, and dialysis
-
recombinant wild-type and mutant enzymes 450fold from Escherichia coli and 25fold from Saccharomyces cerevisiae by ion exchange and immobilized metal affinity chromatography, and gel filtration
P10691
partial
-
native enzyme partially by anion exchange chromatography
-
2 isozymes: SS1 and SS2
-
isolation of corresponding mRNA
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis
P49040, Q00917, Q9FHU4
expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciens
P49040, Q00917, Q94CC8, Q9FHU4, Q9FX32, Q9M111
expression in Escherichia coli
Arachnis hookeriana x Ascocenda Madame Kenny
-
2 genotypes ICPL 84023 and ICP 301 tolerant to waterlogging stress, and 2 genotypes ICP 7035 and Pusa 207 susceptible to waterlogging stress. Pattern of variation in reducing sugar content in the 4 genotypes is parallel to sucrose synthase activity. ICPL 84023 and ICP 301 also show fewer declines in total and non-reducing sugars and greater increase in reducing sugar and SuSy activity than ICP 7035 and Pusa 207
-
genotyping of wild-type and thermotolerant lines
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q9SLY2
DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q0E7D4
enzyme form CaSUS1; enzyme form CaSUS2
-
gene CmSS1, DNA and amino acid sequence determination and analysis, RT-PCR, RACE, and real time PCR analysis
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
O49845
DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q00P15, Q00P16
DNA and amino acid sequence determination and analysis, phylogenetic analysis
P13708
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q9XGB7
gene Sus, DNA and amino acid sequence determination and analysis, tissue-specific expression analysis
-
Nicotiana tabacum cv. xanthi.transgenic plants expressing either gene under the control of a tandem repeat cauliflower mosaic virus 35S promoter (2*35S) or a xylem-localized 4CL promoter (4-coumarate:CoA ligase) are generated. SuSy has the potential to increase overall plant growth and thus increase the total cellulose yield attainable from an individual plant
-
overexpression of the Gossypium hirsutum SuSy gene under control of two promoters in hybrid poplar Populus alba x grandidentata leading to significantly increased SuSy enzyme activity in developing xylem and to increased secondary cell wall cellulose content
-
gene MtSucS1, expression analysis in wild-type and antisense plants
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
P31924
gene SUS1, expression analysis in various rice tissues using real-time quantitative RT-PCR; gene SUS2, expression analysis in various rice tissues using real-time quantitative RT-PCR; gene SUS3, expression analysis in various rice tissues using real-time quantitative RT-PCR; gene SUS4, expression analysis in various rice tissues using real-time quantitative RT-PCR; gene SUS5, expression analysis in various rice tissues using real-time quantitative RT-PCR; gene SUS6, expression analysis in various rice tissues using real-time quantitative RT-PCR
-, P30298, P31924, Q0JE91, Q10LP5, Q43009, Q6K973
DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis; DNA and amino acid sequence determination and analysis, phylogenetic analysis
O24301, O81610, Q9AVR8, Q9T0M9
isoforms Sus1-3
-
gene SuSy, quantitative real-time PCR in wild-type and transgenic plant leaves
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
P49037
constitutive expression in Saccharomyces cerevisiae strain 22574dsus1
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
Q84T18
gene sus1, expression of wild-type enzyme and mutant S11D in Escherichia coli strain BL21(DE3) reveals monosaccharides D-ribulose, D-tagatose, L-glucose, and L-rhamnose as additional acceptor substrates, expression of wild-type SuSy1 and SuSy1 S11A mutant in Saccharomyces cerevisiae strain BY4741 with phosphorylation of the wild-type enzyme at Ser11
P10691
overexpression of SuSy in transgenic plants
-
cloning of sucrose synthase gene fragments. Sorghum sucrose synthase gene fragment I shares homology with other cereal sucrose synthase at the exon positions 6, 7, 8, 9 and 10. Sorghum sucrose synthase fragment II shares homology from exon 2 to 6
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
expression in Escherichia coli
-
production of recombinant His6-SUS2 protein (rSUS2) in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression of isoform SS1 is upregulated by anoxia and by cold temperatures; expression of isoform SS1 is upregulated by anoxia and by cold teperatures; expression of isoform SS3 is upregulated by water deprivation and by cold teperatures
C6H0M2, F8SM21, -, P31922
abscisic acid, exogenous sucrose, as well as treatments like spikelet thinning, leaf cutting increase enzyme expression and enzyme activity during rice grain filling, determination of protein, overview
-
enzyme activity increases by 20-200% for 10-20 days of plant growth in presence of (NH4)2SO4 as compared with the roots of plants growing without nitrogen, induction is higher under illumination by sunlight
-
enzymic activity is higher in immature internodes than in mature internodes in all cultivars, sucrose synthase transcript expression shows a similar pattern. Sucrose synthase activity is negatively correlated with sucrose and positively correlated with hexose sugars
P31925
root restriction treatment increases the enzyme expression, while it reduces cytochrome pathway, and alternative pathway respirations with reduced ATP content. The ratio of invertase/sucrose synthase activity is increased in the restricted roots together with a decrease in glucose content and an increase in fructose content
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S11A
P13708
phosphorylation still occurs, but weakly
S11C
P13708
phosphorylation still occurs, but weakly
S11A
P10691
site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Saccharomyces cerevisiae
S11D
P10691
site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Escherichia coli
additional information
-
activity and specific activity of sucrose synthase in podwalls are significantly higher in genetically modified temperature-tolerant lines 5012, 5014 and 5039 compared to wild-type ICCV 96029 and 96030, overview
additional information
P13708
N-terminal truncation, phosphorylations still occurs, but weakly
S11D
P13708
phosphorylation still occurs, but weakly
additional information
-
silencing of Sus expression in the endosperm by RNAi disrupts its cellularisation process and inhibits early seed development
additional information
-
construction of trangenic antisense construct expressing plants, that display up to 10fold reduced SucS1 levels in roots anf reduced arbuscular mycorrhiza. Mycorrhizal MtSucS1-reduced lines exhibit an overall stunted aboveground growth under phosphorus limitation. The transgenic plants show, apart from a reduced plant height, shoot weight, and leaf development, a delayed flowering, resulting in a lower seed yield. In addition, the root-to-shoot and root weight ratios increase significantly. Major reversion of arbuscular mycorrhiza-associated transcription, exhibiting a significant repression of well-known plant arbuscular mycorrhiza marker and mycosymbiont genes, together indicating a diminished AM fungus colonization of MtSucS1-antisense lines, phenotype, overview
additional information
-
overexpression of the Gossypium hirsutum SuSy gene under control of two promoters in hybrid poplar Populus alba x grandidentata leads to significantly increased SuSy enzyme activity in developing xylem and to increased secondary cell wall cellulose content
additional information
-
root restriction treatment increases the enzyme expression, while it reduces cytochrome pathway, and alternative pathway respirations with reduced ATP content. The ratio of invertase/sucrose synthase activity is increased in the restricted roots together with a decrease in glucose content and an increase in fructose content
additional information
-
construction of transgenic enzyme overexpressing plants and of transgenic antisense plants. ADPG diphosphorylase and UDPG diphosphorylase activities in tubers from overexpressing plants are significantly higher than in control tubers, whereas ADPG diphosphorylase activities in tubers of one SuSy line are significantly lower than in control tubers
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
-
sucrose synthase is a good physiological indicator for use in breeding for improved seed size in chickpea