Information on EC 2.4.1.129 - peptidoglycan glycosyltransferase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.4.1.129
-
RECOMMENDED NAME
GeneOntology No.
peptidoglycan glycosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-diphosphoundecaprenol + undecaprenyl diphosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Peptidoglycan biosynthesis
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peptidoglycan biosynthesis I (meso-diaminopimelate containing)
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peptidoglycan biosynthesis II (staphylococci)
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peptidoglycan biosynthesis III (mycobacteria)
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peptidoglycan biosynthesis IV (Enterococcus faecium)
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peptidoglycan biosynthesis V (beta-lactam resistance)
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SYSTEMATIC NAME
IUBMB Comments
[poly-N-acetyl-D-glucosaminyl-(1->4)-(N-acetyl-D-muramoylpentapeptide)]-diphosphoundecaprenol:[N-acetyl-D-glucosaminyl-(1->4)-N-acetyl-D-muramoylpentapeptide]-diphosphoundecaprenol disaccharidetransferase
The enzyme also works when the lysine residue is replaced by meso-2,6-diaminoheptanedioate (meso-2,6-diaminopimelate, A2pm) combined with adjacent residues through its L-centre, as it is in Gram-negative and some Gram-positive organisms. The undecaprenol involved is ditrans,octacis-undecaprenol (for definitions, click here). Involved in the synthesis of cell-wall peptidoglycan.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
bactoprenyldiphospho-N-acetylmuramoyl-(N-acetyl-D-glucosaminyl)-pentapeptide:peptidoglycan N-acetylmuramoyl-N-acetyl-D-glucosaminyltransferase
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class A penicillin-binding protein
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class A penicillin-binding protein
Escherichia coli BAS849
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ftsI +AHw-gene name+AHwAfA-
G3XD46
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glycosyltransferase, peptidoglycan
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glycosyltransferase/acyltransferase penicillin-binding protein 4
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MGT
Escherichia coli BAS849
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murein synthase
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PBP 1a
Q4TUQ8
isoform
PBP 2b
P0A3M5
isoform
PBP 2x
P14677
isoform
PBP 3
O85665
-
PBP-1A
-
isoform
PBP-1B
-
isoform
PBP-1C
-
isoform
PBP-2
-
isoform
PBP1
-
isoform
PBP1a
-
isoform
PBP1a
O66874
-
PBP1a
-
isoform
PBP1a
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the enzyme has both transglycosylase and transpeptidase activity
PBP1a
Escherichia coli BAS849
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isoform
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PBP1a
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isoform
PBP1a
Q9WZY8
-
PBP1b
-
-
-
-
PBP1b
-
the enzyme has both transglycosylase and transpeptidase activity
PBP1b
Escherichia coli BAS849
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isoform
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PBP1c
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isoform
PBP1c
Escherichia coli BAS849
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isoform
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PBP2
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isoform
PBP2
Staphylococcus aureus NCTC 8325
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-
-
PBP2A
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isoform
PBP2b
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isoform
PBP2c
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isoform
PBP2d
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isoform
PBP3
-
isoform
PBP4
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isoform
PBP7
-
isoform
PBP8
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isoform
penicillin binding protein 1b
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penicillin-binding protein
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penicillin-binding protein
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penicillin-binding protein
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penicillin-binding protein
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penicillin-binding protein
P0A3M5, Q4TUQ8
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penicillin-binding protein 1a
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the enzyme has both transglycosylase and transpeptidase activity
penicillin-binding protein 1a
Q9WZY8
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penicillin-binding protein 1B
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-
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penicillin-binding protein 1B
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penicillin-binding protein 1B
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bifunctional transglycosylase
penicillin-binding protein 1B
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the enzyme has both transglycosylase and transpeptidase activity
penicillin-binding protein 2
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bifunctional peptidoglycan glycosyltransferase/transpeptidase
penicillin-binding protein 2
Staphylococcus aureus NCTC 8325
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bifunctional peptidoglycan glycosyltransferase/transpeptidase
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penicillin-binding protein 3
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penicillin-binding protein 3
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penicillin-binding protein 3
O85665
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penicillin-binding protein 3
G3XD46
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peptidoglycan transglycosylase
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-
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PG-II
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-
-
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PGT
O66874
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CAS REGISTRY NUMBER
COMMENTARY
79079-04-2
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 899
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-
Manually annotated by BRENDA team
Bacillus megaterium 899
strain 899
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-
Manually annotated by BRENDA team
strains 16 M and 16 MDELTApbp1C
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-
Manually annotated by BRENDA team
isolated glycosyltransferase module of PBP1b
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-
Manually annotated by BRENDA team
K12, strains JST975srev61/pLC26-6 (F- mrcB mreA recA)
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Manually annotated by BRENDA team
strain BAS849
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Manually annotated by BRENDA team
strain JA200/pLC19-19
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-
Manually annotated by BRENDA team
strains LMC500 and EJ801
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-
Manually annotated by BRENDA team
Escherichia coli BAS849
strain BAS849
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-
Manually annotated by BRENDA team
Escherichia coli JA200/pLC19-19
strain JA200/pLC19-19
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-
Manually annotated by BRENDA team
strain ATCC 49247 and beta-lactamase-negative ampicillin-resistant and beta-lactamase-positive amoxicillin-clavulanic acid-resistant strains
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Manually annotated by BRENDA team
strain SM1, synonym M. lysodeikticus
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-
Manually annotated by BRENDA team
Micrococcus luteus SM1
strain SM1, synonym M. lysodeikticus
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-
Manually annotated by BRENDA team
strain NCTC 8325
-
-
Manually annotated by BRENDA team
strain SAK 101
-
-
Manually annotated by BRENDA team
Staphylococcus aureus NCTC 8325
strain NCTC 8325
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-
Manually annotated by BRENDA team
Staphylococcus aureus SAK
strain SAK 101
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Manually annotated by BRENDA team
clonal groups Poland23F-16 (strain ATCC BAA-343) and Poland6B-20 (strain ATCC BAA-612)
UniProt
Manually annotated by BRENDA team
clonal groups Poland23F-16 (strain ATCC BAA-343) and Poland6B-20 (strain ATCC BAA-612)
SwissProt
Manually annotated by BRENDA team
strain R6cwl
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-
Manually annotated by BRENDA team
Streptococcus pneumoniae R6cwl
strain R6cwl
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Manually annotated by BRENDA team
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.8
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme G242A
293142
3.6
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme E290Q
293142
4.7
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme H240Q
293142
5.6
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme D234N
293142
6.3
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme S266A
293142
9.2
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme N312A
293142
12.2
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme R372A
293142
36.5
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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mutant enzyme K287A
293142
39
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala-D-Ala)]n-diphosphoundecaprenol
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wild type enzyme PBP1b
293142
174
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[GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-diphosphoundecaprenol
P02919
purified wild type enzyme
137677
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0098
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(3E,7E,14E)-4,9,9,15,19-pentamethyl-12-methylideneicosa-3,7,14,18-tetraen-1-yl (2R)-3-[[[[(2R,3R,4S,5S,6S)-6-carbamoyl-3-[[(2S,3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-([[3-(trifluoromethyl)phenyl]carbonyl]amino)tetrahydro-2H-pyran-2-yl]oxy]-5-hydroxy-4-([[4-(trifluoromethoxy)-3-(trifluoromethyl)phenyl]carbamoyl]amino)tetrahydro-2H-pyran-2-yl]oxy](hydroxy)phosphoryl]oxy]-2-hydroxypropanoate
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-
0.034
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(3Z)-5-(4-bromophenyl)-3-[(5-nitrofuran-2-yl)methylidene]furan-2(3H)-one
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-
0.0037
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(4Z)-2,5-diphenyl-4-[2-(1,3-thiazol-2-yl)hydrazinylidene]-2,4-dihydro-3H-pyrazol-3-one
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-
0.0093
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4-[(1E)-2-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydronaphthalen-2-yl)prop-1-en-1-yl]benzoic acid
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-
0.00388
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chlorobiphenyl desleucyl vancomycin
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-
0.0015
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chlorobiphenyl vancomycin
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0.000006
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moenomycin A
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-
0.000033
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moenomycin disaccharide
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0.000016
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moenomycin trisaccharide
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TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
45
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about 60% of maximal activity at 20C and about half-maximal activity at 45C
PDB
SCOP
CATH
ORGANISM
Atopobium parvulum (strain ATCC 33793 / DSM 20469 / JCM 10300 / VPI 0546)
Atopobium parvulum (strain ATCC 33793 / DSM 20469 / JCM 10300 / VPI 0546)
Atopobium parvulum (strain ATCC 33793 / DSM 20469 / JCM 10300 / VPI 0546)
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
61000
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gel filtration
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
42
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wild type PBP1b is expressed in stable form at 37C and 42C
60
-
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up to 87% loss of activity within 10 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, in concentrated PEG 6000 solution, stable for several months
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