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Information on EC 2.4.1.123 - inositol 3-alpha-galactosyltransferase and Organism(s) Ajuga reptans and UniProt Accession Q9XGN4
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2.4.1.123 inositol 3-alpha-galactosyltransferaseIUBMB Comments
An enzyme from plants involved in the formation of raffinose and stachyose [cf. EC 2.4.1.67 (galactinol---raffinose galactosyltransferase) and EC 2.4.1.82 (galactinol---sucrose galactosyltransferase)].
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The taxonomic range for the selected organisms is: Ajuga reptans
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
galactinol synthase, gols1, csgols1, tsgols2, lcgols1, bhgols1, lcgols2, xvgols, osgols1, osgols2, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
galactinol synthase
galactosyltransferase, uridine diphosphogalactose-inositol
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GOLS
GS
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-
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inositol 1-alpha-galactosyltransferase
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-
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-
UDP-D-galactose:inositol galactosyltransferase
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UDP-galactose:myo-inositol 1-alpha-D-galactosyltransferase
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uridine diphosphogalactose-inositol galactosyltransferase
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galactinol synthase
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GOLS
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:myo-inositol 3-alpha-D-galactosyltransferase
An enzyme from plants involved in the formation of raffinose and stachyose [cf. EC 2.4.1.67 (galactinol---raffinose galactosyltransferase) and EC 2.4.1.82 (galactinol---sucrose galactosyltransferase)].
CAS REGISTRY NUMBER
COMMENTARY
79955-89-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-galactose + myo-inositol
UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol
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i.e. galactinol
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?
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
reaction is probably reversible
identical with galactinol
r
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction
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-
?
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance
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-
?
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
reaction is probably reversible
identical with galactinol
r
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction
-
-
?
UDP-galactose + myo-inositol
UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol
-
-
i.e. galactinol
-
?
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction
-
-
?
UDP-galactose + myo-inositol
UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
-
assay at, GolS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types
GolS-1 is primarily expressed in the mesophyll, the site of raffinose family oligosaccharides storage
GolS-2 is primarily expressed in the phloem-associated intermediary cells known for their role in raffinose family oligosaccharides phloem loading
source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GOLS1_AJURE
333
0
37882
Swiss-Prot
other Location (Reliability: 3)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
putative serine phosphorylation site at GolS-1 codon position 263
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme
cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sprenger, N.; Keller, F.
Allocation of raffinose family oligosaccharides to transport and storage pools in Ajuga reptans: the roles of two distinct galactinol synthases
Plant J.
21
249-258
2000
Ajuga reptans (Q9XGN3), Ajuga reptans (Q9XGN4), Ajuga reptans
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