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Information on EC 2.4.1.122 - N-acetylgalactosaminide beta-1,3-galactosyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q9JJ05

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IUBMB Comments
The eukaryotic enzyme can act on non-reducing O-serine-linked N-acetylgalactosamine residues in mucin glycoproteins, forming the T-antigen. The bacterial enzyme, found in some pathogenic strains, is involved in biosynthesis of the O-antigen repeating unit.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9JJ05
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
galactosyltransferase, c1galt1, t-synthase, beta-galactosyltransferase, c1galt, core 1 synthase, dc1galt1, core 1 beta3-gal-t, ce-t-synthase, core 1 beta3-gal-transferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
core 1 beta3-Gal-T
-
-
core 1 beta3-Gal-transferase
-
-
galactosyltransferase, uridine diphosphogalactose-mucin beta-(1-3)-
-
-
-
-
O-glycan core 1 UDPgalactose:N-acetyl-alpha-galactosaminyl-R beta3-galactosyltransferase
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-
UDP-Gal:GalNAc-alpha-Ser/Thr beta3-galactosyltransferase
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-
UDP-Gal:N-acetylgalactosaminide mucin:beta1,3-galactosyltransferase
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-
uridine diphosphogalactose-mucin beta-(1-3)-galactosyltransferase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + N-acetyl-alpha-D-galactosaminyl-R = UDP + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R
show the reaction diagram
charge-charge interactions between enzyme and substrate
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-alpha-D-galactose:N-acetyl-alpha-D-galactosaminyl-R beta-1,3-galactosyltransferase (configuration-inverting)
The eukaryotic enzyme can act on non-reducing O-serine-linked N-acetylgalactosamine residues in mucin glycoproteins, forming the T-antigen. The bacterial enzyme, found in some pathogenic strains, is involved in biosynthesis of the O-antigen repeating unit.
CAS REGISTRY NUMBER
COMMENTARY hide
97089-61-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
UDP + A-P-(beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl)T-S-S-A
show the reaction diagram
-
synthetic peptide
-
-
?
UDP-galactose + asialo bovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo bovine submaxillary mucin
show the reaction diagram
-
-
-
?
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-O-(4,4-azo)phenyl-GalNAc-alpha-benzyl
-
-
UDP
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slight inhibition
additional information
-
proline on the C-terminal side of the peptide substrate adjacent to Thr(GalNAc) is inhibitory
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
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-
1.7
N-acetyl-alpha-D-galactosaminyl-benzyl
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-
0.76
N-acetyl-alpha-D-galactosaminyl-phenyl
-
-
0.63
UDP-galactose
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with N-acetyl-D-galactosaminyl-alpha-phenyl
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000042
-
liver
0.0071
-
partially purified enzyme, substrate: N-acetyl-D-galactosaminyl-alpha-benzyl
0.031
-
partially purified enzyme, substrate: A-P-N-acetyl-D-galactosaminyl-T-S-S-A
3.43
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C1GLT_RAT
363
1
42311
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
84000 - 86000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 42000-43000, SDS-PAGE and amino acid sequence determination
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on immobilized asialo-bovine submaxillary gland mucin
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Brockhausen, I.; Mller, G.; Pollex-Kruger, A.; Rutz, V.; Paulsen, H.; Matta, K.L.
Control of O-glycan synthesis: specificity and inhibition of O-glycan core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta 3-galactosyltransferase from rat liver
Biochem. Cell Biol.
70
99-108
1992
Rattus norvegicus
Manually annotated by BRENDA team
Brockhausen, I.; Mller, G.; Merz, G.; Adermann, K.; Paulsen, H.
Control of mucin synthesis: the peptide portion of synthetic O-glycopeptide substrates influences the activity of O-glycan core 1 UDPgalactose:N-acetyl-alpha-galactosaminyl-R beta 3-galactosyltransferase
Biochemistry
29
10206-10212
1990
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Granovsky, M.; Bielfeldt, T.; Peters, S.; Paulsen, H.; Meldal, M.; Brockhausen, J.; Brockhausen, I.
UDPgalactose:glycoprotein-N-acetyl-D-galactosamine 3-beta-D-galactosyltransferase activity synthesizing O-glycan core 1 is controlled by the amino acid sequence and glycosylation of glycopeptide substrates
Eur. J. Biochem.
221
1039-1046
1994
Rattus norvegicus
Manually annotated by BRENDA team
Ju, T.; Cummings, R.D.; Canfield, W.M.
Purification, characterization, and subunit structure of rat core 1 beta1,3-galactosyltransferase
J. Biol. Chem.
277
169-177
2002
Rattus norvegicus
Manually annotated by BRENDA team
Ju, T.; Brewer, K.; D'Souza, A.; Cummings, R.D.; Canfield, W.M.
Cloning and expression of human core 1 beta1,3-galactosyltransferase
J. Biol. Chem.
277
178-186
2002
Caenorhabditis elegans (Q18515), Caenorhabditis elegans, Rattus norvegicus (Q9JJ05), Mus musculus (Q9JJ06), Mus musculus, Homo sapiens (Q9NS00), Homo sapiens
Manually annotated by BRENDA team