Information on EC 2.4.1.122 - glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase

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The expected taxonomic range for this enzyme is: Bilateria

EC NUMBER
COMMENTARY hide
2.4.1.122
-
RECOMMENDED NAME
GeneOntology No.
glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-galactose + glycoprotein N-acetyl-D-galactosamine = UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
mucin core 1 and core 2 O-glycosylation
-
-
Mucin type O-Glycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-galactose:glycoprotein-N-acetyl-D-galactosamine 3-beta-D-galactosyltransferase
The non-reducing O-serine-linked N-acetylgalactosamine residues in mucin glycoproteins can act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
97089-61-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-galactose + A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
UDP + A-P-(beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl)T-S-S-A
show the reaction diagram
-
synthetic peptide
-
-
?
UDP-galactose + A-P-(N-acetyl-D-galactosaminyl)T-S-S-S
UDP + A-P-(beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl)T-S-S-S
show the reaction diagram
-
synthetic peptide
-
-
?
UDP-galactose + Ac-GHA-(GalNAcalpha1-)TSLPVTG-NH2
UDP + Ac-GHA-(Galbeta1-3GalNAcalpha1-)TSLPVTG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 750% activity in H292-cells, 550% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-SCSTGHA-(GalNAcalpha1-)TSG-NH2
UDP + Ac-SCSTGHA-(Galbeta-1-3GalNAcalpha1-)TSG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 50% activity in H292-cells, 40% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-STGHA-(GalNAcalpha1-)TSLPG-NH2
UDP + Ac-STGHA-(Galbeta1-3GalNAcalpha1-)TSLPG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 425% activity in H292-cells, 275% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-SVS-(GalNAcalpha1-)TGHATSG-NH2
UDP + Ac-SVS-(Galbeta1-3GalNAcalpha1-)TGHATSG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 300% activity in H292-cells, 450% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + Ac-TSSVS-(GalNAcalpha1-)TGHAG-NH2
UDP + Ac-TSSVS-(Galbeta1-3GalNAcalpha1-)TGHAG-NH2
show the reaction diagram
-
pH 7.0, 0.2 mM, 400% activity in H292-cells, 4000% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + asialo bovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo bovine submaxillary mucin
show the reaction diagram
-
-
-
?
UDP-galactose + asialo ovine submaxillary mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo ovine submaxillary mucin
show the reaction diagram
UDP-galactose + asialo-agalacto-alpha1-acid glycoprotein
UDP + galactosyl-beta-1,3-asialo-agalacto-alpha1-acid glycoprotein
show the reaction diagram
-
-
-
-
?
UDP-galactose + asialo-agalacto-glycophorin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-agalacto-glycophorin
show the reaction diagram
-
-
-
?
UDP-galactose + asialo-mucin of Cowper's gland
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-mucin of Cowper's gland
show the reaction diagram
-
-
-
?
UDP-galactose + benzyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside
UDP + benzyl 2-(acetylamino)-2-deoxy-3-O-beta-D-galactopyranosyl-alpha-D-galactopyranoside
show the reaction diagram
-
pH 7.0, 2 mM, 100% activity in H292-cells, 100% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + GalNAcalpha-Ser-Cy5
UDP + ?
show the reaction diagram
-
-
-
-
?
UDP-galactose + GalNAcalpha1-O-phenyl
?
show the reaction diagram
the enzyme is capable of utilizing UDP-Gal as the donor for the transfer of Gal residues to GalNAcalpha-1-O-phenyl and GalNAcalpha1-Ser/Thr for synthesizing core 1 structure Galbeta1-3GalNAcalpha1-R
-
-
?
UDP-galactose + ganglioside Gg3
UDP + galactosyl-beta-1,3-ganglioside Gg3
show the reaction diagram
i.e. GalNAcbeta1,4-Galbeta1,4-Glcbeta1-ceramide
-
?
UDP-galactose + ganglioside GM2
UDP + galactosyl-beta-1,3-ganglioside GM2
show the reaction diagram
UDP-galactose + glycophorin
?
show the reaction diagram
-
-
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + immunglobulin A1
?
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-benzyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-benzyl
show the reaction diagram
UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl
UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
show the reaction diagram
UDP-galactose + N-acetyl-D-galactosamine
UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + N-acetyl-D-glucosamine
UDP + beta-D-galactosyl-1,3-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
UDP-galactose + N-acetylgalactosaminitol
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminitol
show the reaction diagram
-
-
-
?
UDP-galactose + p-nitrophenyl-beta-N-acetylgalactosamide
UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-p-nitrophenyl
show the reaction diagram
UDP-galactose + salivary gland mucin 81 N-acetyl-D-galactosamine
UDP + salivary gland mucin 81 D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + TTTV-(GalNAcalpha1-)TPTPTG
UDP + TTTV-(Galbeta1-3GalNAcalpha1-)TPTPTG
show the reaction diagram
-
pH 7.0, 0.2 mM, 50% activity in H292-cells, 90% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + TTTVTP-(GalNAcalpha1-)TPTG
UDP + TTTVTP-(Galbeta1-3GalNAcalpha1-)TPTG
show the reaction diagram
-
pH 7.0, 0.2 mM, 110% activity in H292-cells, 60% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
UDP-galactose + TTTVTPTP-(GalNAcalpha1-)TG
UDP + TTTVTPTP-(Galbeta1-3GalNAcalpha1-)TG
show the reaction diagram
-
pH 7.0, 0.2 mM, 65% activity in H292-cells, 60% activity in A549 cells relative to 1-O-benzyl-N-acetyl-D-galactosamine
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-galactose + glycophorin
?
show the reaction diagram
-
-
-
-
-
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + glycoprotein N-acetyl-D-galactosamine
UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
UDP-galactose + N-acetylgalactosaminide mucin
UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
show the reaction diagram
-
participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins
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-
-
UDP-galactose + salivary gland mucin 81 N-acetyl-D-galactosamine
UDP + salivary gland mucin 81 D-galactosyl-1,3-N-acetyl-D-galactosamine
show the reaction diagram
-
glycosylation pattern of the mucin, overview
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
0.1 mM are included in assay medium
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-O-(4,4-azo)phenyl-GalNAc-alpha-benzyl
-
-
alpha-lactalbumin
-
-
-
UDP
-
slight inhibition
additional information
-
proline on the C-terminal side of the peptide substrate adjacent to Thr(GalNAc) is inhibitory
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cosmc
-
specific molecular chaperone
-
lactacystin
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treatment with 0.01 mM lactacystin results in the increased expression of C1GalT protein
Triton X-100
-
activation and stabilization, unstable without
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
A-P-(N-acetyl-D-galactosaminyl)T-S-S-A
-
-
0.00035 - 0.09
asialo Cowper's gland mucin
5
asialo ovine submaxillary mucin
-
in terms of N-acetylgalactosamine equivalents
-
1.7
N-acetyl-alpha-D-galactosaminyl-benzyl
-
-
0.76
N-acetyl-alpha-D-galactosaminyl-phenyl
-
-
0.02 - 0.63
UDP-galactose
additional information
additional information
-
kinetic modeling, numerical simulation of in vivo Core 1 glycosylation pattern
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000028
recombinant in Sf9 cells
0.000042
-
liver
0.000168
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the extracts of EY13370 third instar larvae have a galactosyltransferase activity of 44% of that of wild-type using para-nitrophenyl-N-acetyl-alpha-galactosaminide as the acceptor
0.00088
-
microsomal membranes, substrate: N-acetyl-D-galactosaminyl-alpha-benzyl
0.00134
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microsomal membranes, substrate: A-P-N-acetyl-D-galactosaminyl-T-S-S-S
0.00428
-
purified enzyme
0.0071
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partially purified enzyme, substrate: N-acetyl-D-galactosaminyl-alpha-benzyl
0.0194
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Golgi membranes
0.031
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partially purified enzyme, substrate: A-P-N-acetyl-D-galactosaminyl-T-S-S-A
0.81
-
purified enzyme
3.43
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
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broad
6.5
-
assay at
6.9
-
-
7
-
assay at
7.2
-
assay at
additional information
-
pI: 6.40
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.9
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-
6.5 - 7.4
assays at; assays at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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low content
Manually annotated by BRENDA team
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human colon cancer cell line LSB
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
-
HPC4-tagged soluble T-synthase, determined by SDS-PAGE
48000
-
gel filtration
50000
-
SDS-PAGE
84000 - 86000
-
gel filtration
90000
-
gel filtration in the presence of Triton X-100
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilization by Triton X-100 or Nonidet P-40 required, unstable without
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
3°C, 0.1% Triton X-100 and 0.1 mg/ml albumin, at least 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on immobilized asialo-bovine submaxillary gland mucin
-
affinity chromatography on Sepharose 4B containing covalently bound mucin substrate
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partial, recombinant soluble epitope-tagged enzyme from 293T cell medium
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partial, solubilized with 3% Triton X-100
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solubilized with 1% Triton X-100 in 0.1 M NaCl
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of the C-terminal catalytic domain (amino acids 44-388) of C2GalTA into the vector pMT(1B) and transfected into S2 cells
DNA and amino acid sequence determination
DNA and amino acid sequence determination and analysis, transient functional expression in human 293T cells as wild-type and soluble, i.e. secreted into the medium, epitope-tagged protein, stable functional expression of the soluble epitope-tagged enzyme in CHO-K1 and Lec1-CHO cells
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DNA sequence determination, functional expression in human LSC cells; functional expression in human LSC cells
expressed in HEK-293T cells
-
expressed in wheat germ cell-free translation system
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expression as FLAG-tagged proteins in Sf9 insect cells
-
functional expression in Spodoptera frugiperda Sf9cells via baculovirus infection
generation of mutant mice, oocyte-specific deletion of T-synthase
-
soluble T-synthase is made by co-expressing N-terminal HPC4 epitope-tagged soluble T-synthase with wild-type full-length Cosmc in Hi-5 insect cells using the Baculovirus system
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the chaperone Cosmc promotes renaturation of denatured T-synthase in vitro, HPC4-tagged soluble T-synthase is denatured in 1 ml of 6 M guanidinium hydrochloride, pH 7.2 for 90 min at room temperature, the sample is diluted 100times in reconstitution buffer (10 mM HEPES, 150 mM NaCl, 12 mM MgCl2, pH 7.8), reconstitution is initiated by the addition of 6His-sCosmc and ATP where the final concentration is 2.27 microM and 5 mM, reaction is incubated for 45 min at room temperature, 6His-sCosmc can cause up to 75% restoration of heat-denatured HPC4-tagged soluble T-synthase activity depending on how long the protein is heat-treated, and that it can restore 30% of activity to HPC4-tagged soluble T-synthase denatured by guanidinium hydrochloride treatment
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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polymorphisms of the C1GALT1 gene are associated with the genetic susceptibility to IgA nephropathy in Chinese population