Information on EC 2.4.1.100 - 2,1-fructan:2,1-fructan 1-fructosyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.4.1.100
-
RECOMMENDED NAME
GeneOntology No.
2,1-fructan:2,1-fructan 1-fructosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n = [beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
-
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n = [beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
ping-pong mechansim
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
fructan biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan:(2->1)-beta-D-fructan 1-beta-D-fructosyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,2-beta-D-fructan:1,2-beta-D-fructan 1F-beta-D-fructosyltransferase
-
-
-
-
1,2-beta-fructan 1F-fructosyltransferase
-
-
-
-
1-FFT
-
-
-
-
1-FFT
-
-
1-FFT
Q0PCC9
-
1-FFT
Q4AEI9
-
6G-FFT/1-FFT
Q8S337
-
FFT
-
-
-
-
fructan:fructan 1-fructosyl transferase
-
-
-
-
fructan:fructan 1-fructosyl transferase
-
-
fructan:fructan 1-fructosyltransferase
-
-
fructan:fructan 1-fructosyltransferase
-
-
fructan:fructan 1-fructosyltransferase
Q4AEI9
-
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
Q8S337
-
fructan:fructan fructosyl transferase
-
-
-
-
fructosyltransferase, 1,2-beta-D-fructan 1F-
-
-
-
-
high DP 1-FFT
Q0PCC9
-
high DP fructan:fructan 1-fructosyl transferase
Q0PCC9
-
high DP fructan:fructan 1-fructosyl transferase
-
-
CAS REGISTRY NUMBER
COMMENTARY
73379-55-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
enzyme activity increases during first 14 weeks of storage of plant
-
-
Manually annotated by BRENDA team
burdock
-
-
Manually annotated by BRENDA team
strain HLA-7
-
-
Manually annotated by BRENDA team
Asparagus officinalis HLA-7
strain HLA-7
-
-
Manually annotated by BRENDA team
L. var. foliosum cv. Flash
-
-
Manually annotated by BRENDA team
Helianthus tuberosus Colombia
Colombia
-
-
Manually annotated by BRENDA team
perennial ryegrass
SwissProt
Manually annotated by BRENDA team
gaudin
-
-
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
-
Manually annotated by BRENDA team
Taraxacum officinale Weber
Weber
-
-
Manually annotated by BRENDA team
bifunctional enzyme with additional activity of EC 2.4.1.99, expression in Saccharomyces cerevisisae
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
fructan-fructan 1-fructosyltransferase mediates linear beta(2->1) linkage elongation of 1-kestose and bifurcose to produce mixed levan
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,1,1-kestopentaose
inulin
show the reaction diagram
-
-
oligomers of up to DP 7
-
?
1,1-kestotetraose
inulin
show the reaction diagram
-
-
oligomers of up to DP 7
-
?
1,1-nystose + 1,1-nystose
sucrose + 1-kestose + oligofructan DP5 + oligofructan DP6
show the reaction diagram
-
-
-
?
1-kestose
1F,6G-di-beta-D-fructofuranosylsucrose + nystose
show the reaction diagram
-
-
-
-
?
1-kestose
1F,6G-di-beta-D-fructofuranosylsucrose + nystose
show the reaction diagram
-
-
plus a small amount of neokestose
-
?
1-kestose
?
show the reaction diagram
-
low affinity substrate
-
-
?
1-kestose
1,1-nystose + ?
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
1,1-nystose + sucrose + oligofructans DP5 and DP6
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
inulin-type tetra- and pentasaccharides
?
1-kestose + 1-kestose
D-sucrose + 1,1-nystose
show the reaction diagram
-, Q8S337
1-FFT activity
-
-
?
1-kestose + 6-kestose
1,6-nystose + 6,1-nystose
show the reaction diagram
-
-
-
?
1-kestotriose
inulin
show the reaction diagram
-
-
oligomers of up to DP 6
-
?
1-kestotriose
1,1-kestotetraose + ?
show the reaction diagram
-
the enzyme transfers fructose moieties from and to 1-kestotriose or larger fructans
-
-
?
1-kestotriose + sucrose
1,1-nystose
show the reaction diagram
Q4AEI9, -
the wild type enzyme clearly prefers 1-kestotriose over sucrose
-
-
?
2 1-kestose
inulin + H2O
show the reaction diagram
-
-
up to DP 14
-
?
6-kestose + 1-kestose + sucrose
mixture of tetrasacharides of beta-2,6- and beta-2,1-linked fructans
show the reaction diagram
-
-
-
?
6G-kestose + 6G-kestose
?
show the reaction diagram
-
-
-
-
?
6G-kestose + sucrose
tetrasaccharide
show the reaction diagram
-
-
some synthesis of 1-kestose and resynthesis of 6G-kestose also occurs
?
D-Fructose
?
show the reaction diagram
-
low affinity substrate
-
-
?
fructose
?
show the reaction diagram
-
-
-
-
?
fructose + inulin
oligosaccharide
show the reaction diagram
-
-
reducing fructofuranosyl-only oligosaccharides
?
nystose
1F(1-beta-D-fructofuranosyl)2-6G-beta-D-fructofuranosysucrose + 1F(1-beta-D-fructofuranosyl)3sucrose
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
low affinity substrate
-
-
?
sucrose + 1,1-nystose
?
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1-kestose + 6G-kestose + tetrasaccharide
show the reaction diagram
-
-
transfer of fructosyl residue from 1-kestose to sucrose results in resynthesis of 1-kestose. Tetrasaccharides and 6G-kestose are also synthesized
?
sucrose + inulin
oligosaccharide
show the reaction diagram
-
-
-
-
?
sucrose + inulin
oligosaccharide
show the reaction diagram
-
-
as a series of non-reducing beta-(2, 1)-glucofructo-oligosaccharides
?
sucrose + oligofructan
?
show the reaction diagram
-
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Polymnia sonchifolia
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-, Q0PCC9
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
the enzyme produces tetrasaccharides and higher polymers from trisaccharide
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose is an efficient donor of fructosyl units to sucrose
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
enzyme is specific for fructosyl transfer from beta-2,1-linked 1-kestose or fructan to sucrose and beta-2,1-fructosyl transfer to other fructans
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose-dependent nystose production
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose-dependent nystose production, active on different oligofructans of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
transfers fructosyl groups from oligofructans (degree of polymerization: 3-8) of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Q2WEC6
inulins with degree of polymerization above 6 are much better substrates than sucrose or oligo-fructans with a lower degree of polymerization
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Q4AEI9, -
the enzyme preferentially uses fructan as the donor substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Taraxacum officinale Weber
-
1-kestose-dependent nystose production, active on different oligofructans of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Helianthus tuberosus Colombia
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
role of enzyme in synthesis of fructan
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Polymnia sonchifolia
-
the enzyme might be involved in chain length distribution of the fructan molecules found in rhizophores and in tuberous roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
in combination with the purified chicory root sucrose:sucrose 1-fructosyl transferase, i.e. EC 2.4.1.99, the enzyme synthesizes a range of naturally occuring chicory fructans from sucrose as the sole substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
EC 2.4.1.100, EC 2.4.1.99 and EC 3.2.1.26 simultaneously control fructan in young chicory roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Helianthus tuberosus Colombia
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
inulin
?
show the reaction diagram
-
high affinity substrate
-
-
-
additional information
?
-
-
enzyme is inactive when incubated individually with sucrose, in combination EC 2.4.1.99 and EC 2.4.1.100 can synthesize long-chain inulins in vitro from sucrose
-
-
-
additional information
?
-
-
fructofuranosyl-only oligosaccharides can be synthesized in vitro from fructose and inulin
-
-
-
additional information
?
-
-
plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb
-
-
-
additional information
?
-
-
species-specific fructan pattern within the Asteraceae can be explained by the different charcteristics of their respective 1-FFT enzymes
-
-
-
additional information
?
-
Q2WEC6
the enzyme shows no invertase activity and sucrose: sucrose 1-fructosyl transferase (EC 2.4.1.99) activity in vitro
-
-
-
additional information
?
-
-, Q8S337
a minor 1-SST activity is only found with D-sucrose as a single substrate if there is no 1-kestose available, while 1-kestose hydrolytic activity becomes gradually more important at low concentrations
-
-
-
additional information
?
-
-, Q8S337
the bifunctional 6G-FFT/1-FFT uses 1-kestotriose as a preferential donor substrate and can create both a beta(2->1) linkage between two fructosyl residues and a beta(2->6) linkage between one fructosyl residue and one glucosyl residue
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
role of enzyme in synthesis of fructan
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Polymnia sonchifolia
-
the enzyme might be involved in chain length distribution of the fructan molecules found in rhizophores and in tuberous roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
in combination with the purified chicory root sucrose:sucrose 1-fructosyl transferase, i.e. EC 2.4.1.99, the enzyme synthesizes a range of naturally occuring chicory fructans from sucrose as the sole substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
EC 2.4.1.100, EC 2.4.1.99 and EC 3.2.1.26 simultaneously control fructan in young chicory roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Helianthus tuberosus Colombia
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
additional information
?
-
-
plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ag+
-
1 mM, 100% inhibition
CuSO4
-
1 mM, 22% inhibition
EDTA
-
1 mM, 25% inhibition
p-chloromercuribenzoate
-
0.1 mM, 97% inhibition
Sodium dodecyl sulfate
-
1 mM, 45% inhibition
Sucrose
-
competitive inhibitor of donor substrates at 8-50 mM
Sucrose
-
competitive inhibitor at high concentrations
Hg2+
-
1 mM, 100% inhibition
additional information
-
cell wall/vacuolar inhibitor of fructosidase (0.001 mg) does not inhibit 1-FFT activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Sodium deoxycholate
-
-
Triton X-100
-
-
Tween-80
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
152
-
1,1-nystose
-
-
18
-
1-kestose
-
pH 5.5, 30C
119
-
1-kestose
-
-
233
-
1-kestotriose
Q4AEI9, -
wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30C
377
-
1-kestotriose
Q4AEI9, -
mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30C
976
-
D-sucrose
-, Q8S337
mutant enzyme N340D/W343R/S415N, in 50 mM sodium acetate buffer, pH 5.0, at 30C
199
-
fructose
-
-
440
-
nystose
-
pH 5.5, 30C
197
-
Sucrose
Q4AEI9, -
mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30C
203
-
Sucrose
Q4AEI9, -
wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
15
-
Sucrose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.011
-
-
in 50 mM Na-acetate, pH 5.2, and 0.02% Na-azide for 60 min at 30C
11.63
-
-
pH 5.5, 30C
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.6
8
-
isoelectric focusing
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
-
pH 5.0: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
40
-
5C: about 70% of maximal activity, 40C: about 75% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
inner leaf base
Manually annotated by BRENDA team
-
effect of nitrogen concentration
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
Manually annotated by BRENDA team
-
present in all the developmental phases and regions of the rhizophore. Higher values in the distal region which decreased towards the proximal region. Low 1-FFT activity in the vegetative phase and high activity in the sprouting phase
Manually annotated by BRENDA team
-
tuberous root. Continuous decline of fructooligosaccharides of low degree of polymerization during storage is mainly due to the activity of fructan 1-exohydrolase and less due to the activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase
Manually annotated by BRENDA team
-
green spear. Activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
Manually annotated by BRENDA team
Asparagus officinalis HLA-7
-
green spear. Activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
-
Manually annotated by BRENDA team
Helianthus tuberosus Colombia
-
-
-
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
gel filtration
66000
-
-
gel filtration
69000
-
-
gel filtration
72800
-
-
non-denaturing gel electrophoresis
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 49000, SDS-PAGE
?
Taraxacum officinale Weber
-
x * 49000, SDS-PAGE
-
dimer
-
1 * 17000 + 1 * 52000, SDS-PAGE
dimer
-, Q0PCC9
1 * 19000 + 1 * 52000, SDS-PAGE
monomer
-
1 * 70000, SDS-PAGE
monomer
Helianthus tuberosus Colombia
-
1 * 70000, SDS-PAGE
-
additional information
-
N-terminal amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
glycoprotein
-
-
glycoprotein
Taraxacum officinale Weber
-
-
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.3
6.3
-
30C, stable for 30 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
40
-
stable for 15 min
30
-
-
1 h, stable
40
-
-
1 h, up to 20% loss of activity below
50
-
-
inactivation above
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
several attempts to purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium
-, Q8S337
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1C, 8 weeks, an increase in 1-FFT activity is detected
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
-, Q0PCC9
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeasts expression medium
-, Q8S337
Fast Desalting column chromatography
Q4AEI9, -
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in leaves of Nicotiana benthamiana
-
expression in Pichia pastoris. The recombinant enzyme is a functional 1-FFT lacking invertase and sucrose:sucrose 1-fructosyl transferase activities, but shows a small intrinsic fructan exohydrolase activity
-, Q0PCC9
expressed in Pichia pastoris
-, Q8S337
expressed in Pichia pastoris
Q4AEI9, -
heterologous expression in Pichia pastoris
Q2WEC6
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
treatment with the calcium channel blocker LaCl3 prevents the induction of 1-FFT, the calmodulin antagonist W7 decreases the induction of 1-FFT, genistein and staurosporine reduce the expression of 1-FFT, endothal and cantharidin strongly repress the induction of 1-FFT in a very similar manner
-
increasing the carbon availability by increasing the sucrose concentration from 3 to 6% (w/v) does not result in sustained 1-FFT induction, LY-294002 does not affect the induction of 1-FFT expression
-
expression of 1-FFT is strongly induced upon transfer to high-carbon/low-nitrogen medium, limiting nitrogen supply by lowering KNO3 and (NH4)2SO4 concentrations to 5 and 0.2 mM, respectively, under an elevated sucrose concentration (6%), results in a strong increase in transcript level for 1-FFT, 1-FFT expression is transiently and only moderately induced during the first 24 h of cold exposure
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
N340D
-, Q8S337
the mutant shows only very low overall activity levels
N340D/S415N
-, Q8S337
the mutant shows similar properties to the W343R mutant, but the 1-nystose/neoketose ratio is decreased
N340D/W343R
-, Q8S337
the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants
N340D/W343R/S415N
-, Q8S337
the triple mutant gains the characteristic of using sucrose as a donor substrate, at the same time, this mutant can still use 1-kestose as a donor substrate, but in a much less efficient way than the wild type
S415N
-, Q8S337
the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected
W343R
-, Q8S337
the mutant shows a wild type1-FFT activity but a largely reduced 6G-FFT activity
D281Y
Q4AEI9, -
the mutation restores the tight H-bond network and introduces typical S-type enzyme characteristics like producing similar amounts of 1-kestotriose compared to sucrose:sucrose 1-fructosyltransferases
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
-
effect of temperature and storage time of plant on enzyme activity
nutrition
-
activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion of asparagus spear and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
nutrition
Asparagus officinalis HLA-7
-
activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion of asparagus spear and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
-
agriculture
-, Q0PCC9
introduction of the high DP 1-FFT gene in chicory, a crop widely used for inulin extraction, could lead to an increase in degree of polymerization which is useful for a number of specific industrial applications
synthesis
-, Q0PCC9
the enzyme might be suitable for both fructo-oligosaccharides and high degree of polymerization inulin production in bioreactors
nutrition
-
continuous decline of fructooligosaccharides of low degree of polymerization during storage of tuberous roots is mainly due to the activity of fructan 1-exohydrolase and less due to the activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase
synthesis
Q2WEC6
because inulin-type fructans with a high degree of polymerization show superior properties for specific food and non-food applications, the hDP 1-FFT gene from Viguiera discolor has potential for the production of inulin with a high degree of polymerization in vitro or in transgenic crops