Information on EC 2.4.1.100 - 2,1-fructan:2,1-fructan 1-fructosyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.4.1.100
-
RECOMMENDED NAME
GeneOntology No.
2,1-fructan:2,1-fructan 1-fructosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n = [beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
-
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n = [beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
ping-pong mechansim
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
fructan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(2->1)-beta-D-fructan:(2->1)-beta-D-fructan 1-beta-D-fructosyltransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1,2-beta-D-fructan:1,2-beta-D-fructan 1F-beta-D-fructosyltransferase
-
-
-
-
1,2-beta-fructan 1F-fructosyltransferase
-
-
-
-
1-FFT
-
-
-
-
1-FFT
-
-
1-FFT
Q0PCC9
-
1-FFT
Q4AEI9
-
6G-FFT/1-FFT
Q8S337
-
FFT
-
-
-
-
fructan:fructan 1-fructosyl transferase
-
-
-
-
fructan:fructan 1-fructosyl transferase
-
-
fructan:fructan 1-fructosyltransferase
-
-
fructan:fructan 1-fructosyltransferase
-
-
fructan:fructan 1-fructosyltransferase
Q4AEI9
-
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase
Q8S337
-
fructan:fructan fructosyl transferase
-
-
-
-
fructosyltransferase, 1,2-beta-D-fructan 1F-
-
-
-
-
high DP 1-FFT
Q0PCC9
-
high DP fructan:fructan 1-fructosyl transferase
Q0PCC9
-
high DP fructan:fructan 1-fructosyl transferase
-
-
CAS REGISTRY NUMBER
COMMENTARY
73379-55-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme activity increases during first 14 weeks of storage of plant
-
-
Manually annotated by BRENDA team
burdock
-
-
Manually annotated by BRENDA team
strain HLA-7
-
-
Manually annotated by BRENDA team
Asparagus officinalis HLA-7
strain HLA-7
-
-
Manually annotated by BRENDA team
L. var. foliosum cv. Flash
-
-
Manually annotated by BRENDA team
Helianthus tuberosus Colombia
Colombia
-
-
Manually annotated by BRENDA team
perennial ryegrass
SwissProt
Manually annotated by BRENDA team
gaudin
-
-
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
-
Manually annotated by BRENDA team
Taraxacum officinale Weber
Weber
-
-
Manually annotated by BRENDA team
bifunctional enzyme with additional activity of EC 2.4.1.99, expression in Saccharomyces cerevisisae
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
fructan-fructan 1-fructosyltransferase mediates linear beta(2->1) linkage elongation of 1-kestose and bifurcose to produce mixed levan
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,1,1-kestopentaose
inulin
show the reaction diagram
-
-
oligomers of up to DP 7
-
?
1,1-kestotetraose
inulin
show the reaction diagram
-
-
oligomers of up to DP 7
-
?
1,1-nystose + 1,1-nystose
sucrose + 1-kestose + oligofructan DP5 + oligofructan DP6
show the reaction diagram
-
-
-
?
1-kestose
1F,6G-di-beta-D-fructofuranosylsucrose + nystose
show the reaction diagram
-
-
-
-
?
1-kestose
1F,6G-di-beta-D-fructofuranosylsucrose + nystose
show the reaction diagram
-
-
plus a small amount of neokestose
-
?
1-kestose
?
show the reaction diagram
-
low affinity substrate
-
-
?
1-kestose
1,1-nystose + ?
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
-
-
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
1,1-nystose + sucrose + oligofructans DP5 and DP6
?
1-kestose + 1-kestose
1,1-nystose + 1,1,1-logose
show the reaction diagram
-
-
inulin-type tetra- and pentasaccharides
?
1-kestose + 1-kestose
D-sucrose + 1,1-nystose
show the reaction diagram
Q8S337
1-FFT activity
-
-
?
1-kestose + 6-kestose
1,6-nystose + 6,1-nystose
show the reaction diagram
-
-
-
?
1-kestotriose
inulin
show the reaction diagram
-
-
oligomers of up to DP 6
-
?
1-kestotriose
1,1-kestotetraose + ?
show the reaction diagram
-
the enzyme transfers fructose moieties from and to 1-kestotriose or larger fructans
-
-
?
1-kestotriose + sucrose
1,1-nystose
show the reaction diagram
Q4AEI9
the wild type enzyme clearly prefers 1-kestotriose over sucrose
-
-
?
2 1-kestose
inulin + H2O
show the reaction diagram
-
-
up to DP 14
-
?
6-kestose + 1-kestose + sucrose
mixture of tetrasacharides of beta-2,6- and beta-2,1-linked fructans
show the reaction diagram
-
-
-
?
6G-kestose + 6G-kestose
?
show the reaction diagram
-
-
-
-
?
6G-kestose + sucrose
tetrasaccharide
show the reaction diagram
-
-
some synthesis of 1-kestose and resynthesis of 6G-kestose also occurs
?
D-Fructose
?
show the reaction diagram
-
low affinity substrate
-
-
?
fructose
?
show the reaction diagram
-
-
-
-
?
fructose + inulin
oligosaccharide
show the reaction diagram
-
-
reducing fructofuranosyl-only oligosaccharides
?
nystose
1F(1-beta-D-fructofuranosyl)2-6G-beta-D-fructofuranosysucrose + 1F(1-beta-D-fructofuranosyl)3sucrose
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
-
-
-
?
sucrose
?
show the reaction diagram
-
low affinity substrate
-
-
?
sucrose + 1,1-nystose
?
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1,1-nystose
show the reaction diagram
-
-
-
-
?
sucrose + 1-kestose
1-kestose + 6G-kestose + tetrasaccharide
show the reaction diagram
-
-
transfer of fructosyl residue from 1-kestose to sucrose results in resynthesis of 1-kestose. Tetrasaccharides and 6G-kestose are also synthesized
?
sucrose + inulin
oligosaccharide
show the reaction diagram
-
-
-
-
?
sucrose + inulin
oligosaccharide
show the reaction diagram
-
-
as a series of non-reducing beta-(2, 1)-glucofructo-oligosaccharides
?
sucrose + oligofructan
?
show the reaction diagram
-
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Polymnia sonchifolia
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Q0PCC9
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
-
the enzyme produces tetrasaccharides and higher polymers from trisaccharide
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose is an efficient donor of fructosyl units to sucrose
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
enzyme is specific for fructosyl transfer from beta-2,1-linked 1-kestose or fructan to sucrose and beta-2,1-fructosyl transfer to other fructans
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose-dependent nystose production
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
1-kestose-dependent nystose production, active on different oligofructans of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
-
transfers fructosyl groups from oligofructans (degree of polymerization: 3-8) of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Q2WEC6
inulins with degree of polymerization above 6 are much better substrates than sucrose or oligo-fructans with a lower degree of polymerization
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Q4AEI9
the enzyme preferentially uses fructan as the donor substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Taraxacum officinale Weber
-
1-kestose-dependent nystose production, active on different oligofructans of the inulin series
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1
show the reaction diagram
Helianthus tuberosus Colombia
-
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
role of enzyme in synthesis of fructan
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Polymnia sonchifolia
-
the enzyme might be involved in chain length distribution of the fructan molecules found in rhizophores and in tuberous roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
in combination with the purified chicory root sucrose:sucrose 1-fructosyl transferase, i.e. EC 2.4.1.99, the enzyme synthesizes a range of naturally occuring chicory fructans from sucrose as the sole substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
EC 2.4.1.100, EC 2.4.1.99 and EC 3.2.1.26 simultaneously control fructan in young chicory roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Helianthus tuberosus Colombia
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
inulin
?
show the reaction diagram
-
high affinity substrate
-
-
-
additional information
?
-
-
enzyme is inactive when incubated individually with sucrose, in combination EC 2.4.1.99 and EC 2.4.1.100 can synthesize long-chain inulins in vitro from sucrose
-
-
-
additional information
?
-
-
fructofuranosyl-only oligosaccharides can be synthesized in vitro from fructose and inulin
-
-
-
additional information
?
-
-
plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb
-
-
-
additional information
?
-
-
species-specific fructan pattern within the Asteraceae can be explained by the different charcteristics of their respective 1-FFT enzymes
-
-
-
additional information
?
-
Q2WEC6
the enzyme shows no invertase activity and sucrose: sucrose 1-fructosyl transferase (EC 2.4.1.99) activity in vitro
-
-
-
additional information
?
-
Q8S337
a minor 1-SST activity is only found with D-sucrose as a single substrate if there is no 1-kestose available, while 1-kestose hydrolytic activity becomes gradually more important at low concentrations
-
-
-
additional information
?
-
Q8S337
the bifunctional 6G-FFT/1-FFT uses 1-kestotriose as a preferential donor substrate and can create both a beta(2->1) linkage between two fructosyl residues and a beta(2->6) linkage between one fructosyl residue and one glucosyl residue
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
role of enzyme in synthesis of fructan
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Polymnia sonchifolia
-
the enzyme might be involved in chain length distribution of the fructan molecules found in rhizophores and in tuberous roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
in combination with the purified chicory root sucrose:sucrose 1-fructosyl transferase, i.e. EC 2.4.1.99, the enzyme synthesizes a range of naturally occuring chicory fructans from sucrose as the sole substrate
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
-
EC 2.4.1.100, EC 2.4.1.99 and EC 3.2.1.26 simultaneously control fructan in young chicory roots
-
-
?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n
?
show the reaction diagram
Helianthus tuberosus Colombia
-
the enzyme is involved in the synthesis of beta-2,1-linked fructose polymers
-
-
?
additional information
?
-
-
plays an important role in the synthesis of inulin and inulinneo-series fructo-oligosaccharides in onion bulb
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ag+
-
1 mM, 100% inhibition
CuSO4
-
1 mM, 22% inhibition
EDTA
-
1 mM, 25% inhibition
p-chloromercuribenzoate
-
0.1 mM, 97% inhibition
Sodium dodecyl sulfate
-
1 mM, 45% inhibition
Sucrose
-
competitive inhibitor of donor substrates at 8-50 mM
Sucrose
-
competitive inhibitor at high concentrations
Hg2+
-
1 mM, 100% inhibition
additional information
-
cell wall/vacuolar inhibitor of fructosidase (0.001 mg) does not inhibit 1-FFT activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Sodium deoxycholate
-
-
Triton X-100
-
-
Tween-80
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
152
1,1-nystose
-
-
18
1-kestose
-
pH 5.5, 30C
119
1-kestose
-
-
233
1-kestotriose
Q4AEI9
wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30C
377
1-kestotriose
Q4AEI9
mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30C
976
D-sucrose
Q8S337
mutant enzyme N340D/W343R/S415N, in 50 mM sodium acetate buffer, pH 5.0, at 30C
199
fructose
-
-
440
nystose
-
pH 5.5, 30C
0.2
Sucrose
-
-
197
Sucrose
Q4AEI9
mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30C
203
Sucrose
Q4AEI9
wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
15
Sucrose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.011
-
in 50 mM Na-acetate, pH 5.2, and 0.02% Na-azide for 60 min at 30C
11.63
-
pH 5.5, 30C
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 6.5
-
-
5.6 - 8
-
isoelectric focusing
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8
-
pH 5.0: about 70% of maximal activity, pH 8.0: about 65% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 40
-
5C: about 70% of maximal activity, 40C: about 75% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
inner leaf base
Manually annotated by BRENDA team
-
effect of nitrogen concentration
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
Manually annotated by BRENDA team
-
present in all the developmental phases and regions of the rhizophore. Higher values in the distal region which decreased towards the proximal region. Low 1-FFT activity in the vegetative phase and high activity in the sprouting phase
Manually annotated by BRENDA team
-
tuberous root. Continuous decline of fructooligosaccharides of low degree of polymerization during storage is mainly due to the activity of fructan 1-exohydrolase and less due to the activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase
Manually annotated by BRENDA team
-
green spear. Activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
Manually annotated by BRENDA team
Asparagus officinalis HLA-7
-
green spear. Activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
-
Manually annotated by BRENDA team
Helianthus tuberosus Colombia
-
-
-
Manually annotated by BRENDA team
Polymnia sonchifolia
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
-
gel filtration
488350
66000
-
gel filtration
660021
69000
-
gel filtration
488355
70000
-
-
488352
72800
-
non-denaturing gel electrophoresis
488347
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 49000, SDS-PAGE
?
Taraxacum officinale Weber
-
x * 49000, SDS-PAGE
-
dimer
-
1 * 17000 + 1 * 52000, SDS-PAGE
dimer
Q0PCC9
1 * 19000 + 1 * 52000, SDS-PAGE
monomer
-
1 * 70000, SDS-PAGE
monomer
Helianthus tuberosus Colombia
-
1 * 70000, SDS-PAGE
-
additional information
-
N-terminal amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
-
-
glycoprotein
-
-
glycoprotein
Taraxacum officinale Weber
-
-
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.3 - 6.3
-
30C, stable for 30 min
660021
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20 - 40
-
stable for 15 min
660021
30
-
1 h, stable
488348
40
-
1 h, up to 20% loss of activity below
488347
50
-
inactivation above
660021
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
several attempts to purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium
Q8S337
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
1C, 8 weeks, an increase in 1-FFT activity is detected
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeasts expression medium
Q8S337
Fast Desalting column chromatography
Q4AEI9
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in leaves of Nicotiana benthamiana
-
expression in Pichia pastoris. The recombinant enzyme is a functional 1-FFT lacking invertase and sucrose:sucrose 1-fructosyl transferase activities, but shows a small intrinsic fructan exohydrolase activity
Q0PCC9
expressed in Pichia pastoris
Q8S337
expressed in Pichia pastoris
Q4AEI9
heterologous expression in Pichia pastoris
Q2WEC6
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
treatment with the calcium channel blocker LaCl3 prevents the induction of 1-FFT, the calmodulin antagonist W7 decreases the induction of 1-FFT, genistein and staurosporine reduce the expression of 1-FFT, endothal and cantharidin strongly repress the induction of 1-FFT in a very similar manner
-
increasing the carbon availability by increasing the sucrose concentration from 3 to 6% (w/v) does not result in sustained 1-FFT induction, LY-294002 does not affect the induction of 1-FFT expression
-
expression of 1-FFT is strongly induced upon transfer to high-carbon/low-nitrogen medium, limiting nitrogen supply by lowering KNO3 and (NH4)2SO4 concentrations to 5 and 0.2 mM, respectively, under an elevated sucrose concentration (6%), results in a strong increase in transcript level for 1-FFT, 1-FFT expression is transiently and only moderately induced during the first 24 h of cold exposure
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
N340D
Q8S337
the mutant shows only very low overall activity levels
N340D/S415N
Q8S337
the mutant shows similar properties to the W343R mutant, but the 1-nystose/neoketose ratio is decreased
N340D/W343R
Q8S337
the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants
N340D/W343R/S415N
Q8S337
the triple mutant gains the characteristic of using sucrose as a donor substrate, at the same time, this mutant can still use 1-kestose as a donor substrate, but in a much less efficient way than the wild type
S415N
Q8S337
the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected
W343R
Q8S337
the mutant shows a wild type1-FFT activity but a largely reduced 6G-FFT activity
D281Y
Q4AEI9
the mutation restores the tight H-bond network and introduces typical S-type enzyme characteristics like producing similar amounts of 1-kestotriose compared to sucrose:sucrose 1-fructosyltransferases
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
-
effect of temperature and storage time of plant on enzyme activity
nutrition
-
activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion of asparagus spear and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
nutrition
Asparagus officinalis HLA-7
-
activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase is high in the top portion of asparagus spear and decreases during storage, while in the middle and bottom portions, its activity varies slightly. The ratio of 6G-fructosyltransferase and 2,1-fructan:2,1-fructan 1-fructosyltransferase is independent of temperature
-
agriculture
Q0PCC9
introduction of the high DP 1-FFT gene in chicory, a crop widely used for inulin extraction, could lead to an increase in degree of polymerization which is useful for a number of specific industrial applications
synthesis
Q0PCC9
the enzyme might be suitable for both fructo-oligosaccharides and high degree of polymerization inulin production in bioreactors
nutrition
-
continuous decline of fructooligosaccharides of low degree of polymerization during storage of tuberous roots is mainly due to the activity of fructan 1-exohydrolase and less due to the activity of 2,1-fructan:2,1-fructan 1-fructosyltransferase
synthesis
Q2WEC6
because inulin-type fructans with a high degree of polymerization show superior properties for specific food and non-food applications, the hDP 1-FFT gene from Viguiera discolor has potential for the production of inulin with a high degree of polymerization in vitro or in transgenic crops