Information on EC 2.3.3.6 - 2-ethylmalate synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.3.3.6
-
RECOMMENDED NAME
GeneOntology No.
2-ethylmalate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
acetyl-CoA + H2O + 2-oxobutanoate = (R)-2-ethylmalate + CoA
show the reaction diagram
also acts on (R)-2-(n-propyl)-malate
-
acetyl-CoA + H2O + 2-oxobutanoate = (R)-2-ethylmalate + CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Pyruvate metabolism
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:2-oxobutanoate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
Also acts on (R)-2-(n-propyl)-malate. Formerly wrongly included with EC 2.3.3.7 3-ethylmalate synthase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(R)-2-ethylmalate 2-oxobutanoyl-lyase (CoA-acetylating)
-
-
-
-
2-ethylmalate-3-hydroxybutanedioate synthase
-
-
-
-
4.1.3.33
-
-
formerly
-
propylmalate synthase
-
-
-
-
propylmalic synthase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9024-01-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + H2O + 2-ketoisovalerate
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + H2O + 2-ketovalerate
(R)-2-(n-propyl)-malate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + 2-ketovalerate
(R)-2-(n-propyl)-malate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + 2-oxobutanoate
(R)-2-ethylmalate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + 2-oxobutanoate
(R)-2-ethylmalate + CoA
show the reaction diagram
-
-
-
ir
acetyl-CoA + H2O + pyruvate
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
not: 2-ketocaproate and 2-ketoisocaproate
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
in the absence of Mg2+ product is formed to an extent of 64% in the complete reaction
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
L-isoleucine
-
weak
L-leucine
-
50% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(NH4)2SO4
-
maximum of activity: about 45% (NH4)2SO4 and decrease in activity at higher concentrations
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
product formation doubled between pH 6.0 and 6.5, but there is a broud shoulder of activity between pH 6.5 and 7.5
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
heating in boiling water for 1 min results in a total loss of activity