Information on EC 2.3.3.5 - 2-methylcitrate synthase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.3.3.5
-
RECOMMENDED NAME
GeneOntology No.
2-methylcitrate synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
propanoyl-CoA + H2O + oxaloacetate = (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
; the enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme has been separated from EC 2.3.3.1 citrate (Si) synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
condensation
-
-
-
-
condensation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-methylcitrate cycle I
-
-
2-methylcitrate cycle II
-
-
propionate fermentation
-
-
Propanoate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming)
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 2.3.3.1, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-MC synthase
-
-
2-MC synthase
Salmonella enterica JE2170
-
-
-
2-MCS1
Cupriavidus necator H16
-
-
2-methylcitrate oxaloacetate-lyase
-
-
-
-
2-methylcitrate synthase 2
-
2-methylcitrate synthase 2
Cupriavidus necator H16
-
-
citrate synthase II
-
-
citrate synthase II
Escherichia coli K12
-
-
-
EC 4.1.3.31
-
-
formerly
-
MCA condensing enzyme
-
-
-
-
MCS
-
-
-
-
Methylcitrate synthase
-
-
-
-
Methylcitrate synthase
-
-
Methylcitrate synthase
-
Methylcitrate synthase
-
-
Methylcitrate synthase
-
-
methylcitrate synthetase
-
-
-
-
PrpC
Salmonella enterica JE2170
-
-
-
prpC2
Cupriavidus necator H16
-
-
CAS REGISTRY NUMBER
COMMENTARY
57827-78-8
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
psychrotolerant antarctic eubacterium, strain DS2-3R
-
-
Manually annotated by BRENDA team
opportunistic human pathogen, strain CEA17, strain ATCC 46654
SwissProt
Manually annotated by BRENDA team
wild-type strain CBS 144.89, methylcitrate synthase mutant DELTAmcsA, complemented methylcitrate synthase mutant 11/12 B1
-
-
Manually annotated by BRENDA team
methylcitrate deletion strain RYQ11
-
-
Manually annotated by BRENDA team
strains SMI45 and SMB/acuA, the latter is acetyl-CoA synthase-deficient, gene mscA
-
-
Manually annotated by BRENDA team
IFO 6662
-
-
Manually annotated by BRENDA team
Burkholderia cepacia contig261
contig261
-
-
Manually annotated by BRENDA team
strain IPT101
-
-
Manually annotated by BRENDA team
Burkholderia sacchari IPT101
strain IPT101
-
-
Manually annotated by BRENDA team
CBS 565, IFO 0720, CBS 1904, CBS 2015, CBS 6174, OM-102
-
-
Manually annotated by BRENDA team
IFO 0753
-
-
Manually annotated by BRENDA team
IFO 0661
-
-
Manually annotated by BRENDA team
IFO 0966
-
-
Manually annotated by BRENDA team
ATCC 13032, prp gene cluster
SwissProt
Manually annotated by BRENDA team
formerly Ralstonia eutropha, HF39 prp gene cluster
SwissProt
Manually annotated by BRENDA team
formerly Ralstonia eutropha, HF39 prp gene cluster; formerly Ralstonia eutropha, prp gene cluster
SwissProt
Manually annotated by BRENDA team
formerly Ralstonia eutropha, HF39; Ralstonia eutropha CH34
-
-
Manually annotated by BRENDA team
strain H16
SwissProt
Manually annotated by BRENDA team
strain H16
-
-
Manually annotated by BRENDA team
Cupriavidus necator H16
strain H16
SwissProt
Manually annotated by BRENDA team
Cupriavidus necator H16
strain H16
-
-
Manually annotated by BRENDA team
Cyberlindnera saturnus IAM 4094
IAM 4094
-
-
Manually annotated by BRENDA team
JM109; K12
-
-
Manually annotated by BRENDA team
K12; O157:H17
-
-
Manually annotated by BRENDA team
O157:H17
UniProt
Manually annotated by BRENDA team
prp gene cluster
SwissProt
Manually annotated by BRENDA team
SWISSPROT acc. No.
SwissProt
Manually annotated by BRENDA team
Escherichia coli JM109
JM109
-
-
Manually annotated by BRENDA team
Escherichia coli K12
K12
-
-
Manually annotated by BRENDA team
Escherichia coli O157:H17
O157:H17
-
-
Manually annotated by BRENDA team
Escherichia coli O157:H17
O157:H17
UniProt
Manually annotated by BRENDA team
IFO 0586, IFO 0882
-
-
Manually annotated by BRENDA team
IFO 0678
-
-
Manually annotated by BRENDA team
Mucor rouxianus
IFO 5773
-
-
Manually annotated by BRENDA team
; serogroup A; serogroup B
-
-
Manually annotated by BRENDA team
IFO 6067
-
-
Manually annotated by BRENDA team
no activity in Candida rugosa
IFO 0750, NPA-1, IFO 1364
-
-
Manually annotated by BRENDA team
no activity in Sus scrofa
pig
-
-
Manually annotated by BRENDA team
IFO 0799
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAO
PAO
-
-
Manually annotated by BRENDA team
Pseudomonas fluorescens contig267
contig267
-
-
Manually annotated by BRENDA team
contig10768; KT2440
-
-
Manually annotated by BRENDA team
Pseudomonas putida contig10768
contig10768
-
-
Manually annotated by BRENDA team
contig5376
-
-
Manually annotated by BRENDA team
Pseudomonas syringae contig5376
contig5376
-
-
Manually annotated by BRENDA team
Rhizopus microsporus var. chinensis IAM 6003
IAM 6003
-
-
Manually annotated by BRENDA team
R-10 (DSM 4252)
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae CBS 8066
CBS 8066
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae SANK 50182
SANK 50182
-
-
Manually annotated by BRENDA team
IFO 1711, IFO 1744
-
-
Manually annotated by BRENDA team
serovar typhimurium
GenBank
Manually annotated by BRENDA team
serovar typhimurium LT2
-
-
Manually annotated by BRENDA team
serovar Typhimurium, prp gene cluster
GenBank
Manually annotated by BRENDA team
synonym Salmonella typhimurium
-
-
Manually annotated by BRENDA team
Salmonella enterica JE2170
-
-
-
Manually annotated by BRENDA team
Shewanella putrefaciens contig93
contig93
-
-
Manually annotated by BRENDA team
Ustilago crus-galli
F-B-6
-
-
Manually annotated by BRENDA team
Ustilago crus-galli F-B-6
F-B-6
-
-
Manually annotated by BRENDA team
Ustilago utriculosa
F-B-5
-
-
Manually annotated by BRENDA team
Ustilago utriculosa F-B-5
F-B-5
-
-
Manually annotated by BRENDA team
Candida lipolytica R-2; formerly Candida lipolytica; IFO 1659
-
-
Manually annotated by BRENDA team
formerly Candida lipolytica
-
-
Manually annotated by BRENDA team
formerly Candida lipolytica; IFO 1659
-
-
Manually annotated by BRENDA team
IFO 1659
-
-
Manually annotated by BRENDA team
IFO 1659; synonym Saccharomycopsis lipolytica IFO 1457, IFO 1542
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
methylcitrate cycle
physiological function
-
propionyl-CoA detoxification
physiological function
-
propionyl-CoA detoxification
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
uses acetyl-CoA in preference to propionyl-CoA
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
uses acetyl-CoA in preference to propionyl-CoA
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
-
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
-
ir
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
the enzyme also performs the citrate synthase reaction, EC 2.3.3.1
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
Neurospora crassa IFO 6067
-
uses acetyl-CoA in preference to propionyl-CoA
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
Cupriavidus necator H16
-
-
?
acetyl-CoA + H2O + oxaloacetate
? + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
?
show the reaction diagram
-
-
-
?
n-butyryl-CoA + H2O + oxaloacetate
2-ethylcitrate + CoA
show the reaction diagram
-
-
-
r
n-butyryl-CoA + H2O + oxaloacetate
2-ethylcitrate + CoA
show the reaction diagram
Cupriavidus necator, Cupriavidus necator H16
1.8% of the activity with propanoyl-CoA
-
?
n-valeryl-CoA + H2O + oxaloacetate
2-propylcitrate + CoA
show the reaction diagram
-
-
-
r
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
propanoyl-CoA is the most efficient substrate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Neurospora crassa IFO 6067, Mucor rouxianus IFO 5773
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas putida contig10768
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Candida pararugosa IFO 0966, Rhizopus microsporus var. chinensis IAM 6003, Saccharomyces cerevisiae SANK 50182
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Saccharomyces cerevisiae SANK 50182
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Salmonella enterica JE2170
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Ustilago utriculosa F-B-5
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli JM109
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas syringae contig5376, Shewanella putrefaciens contig93
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Cyberlindnera saturnus IAM 4094
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Burkholderia cepacia contig261, Pseudomonas fluorescens contig267
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
first key enzyme of the methylcitric acid cycle, implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas aeruginosa PAO
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Saccharomyces cerevisiae CBS 8066
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Ustilago crus-galli F-B-6
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Burkholderia sacchari IPT101
-
first key enzyme of the methylcitric acid cycle, implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
show the reaction diagram
-
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
show the reaction diagram
-
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview, reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
-
ir
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
show the reaction diagram
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
show the reaction diagram
Cupriavidus necator H16
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
valeryl-CoA + H2O + oxaloacetate
?
show the reaction diagram
Cupriavidus necator, Cupriavidus necator H16
3% of the activity with propanoyl-CoA
-
-
?
butyryl-CoA + H2O + oxaloacetate
?
show the reaction diagram
-
-
-
?
additional information
?
-
-
constitutive
-
-
-
additional information
?
-
-
constitutive
-
-
-
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
-
additional information
?
-
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
-
additional information
?
-
-
enzyme is specific for oxaloacetate, does not catalyze liberation of CoA from acetyl- or propionyl-CoA in presence of glyoxylate, pyruvate or alpha-ketoglutarate, isobutyryl-CoA and isovaleryl-CoA are no substrates
-
-
-
additional information
?
-
-
enzyme is relatively specific for its acetyl-CoA substrate, utilizing propionyl-CoA and not acetyl-CoA
-
-
-
additional information
?
-
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
-
-
-
additional information
?
-
Escherichia coli JM109, Escherichia coli K12
-
enzyme is induced by growth on propionate, propanol and heptanoate
-
-
-
additional information
?
-
Pseudomonas aeruginosa PAO
-
enzyme is induced by growth on propionate, propanol and heptanoate, enzyme is relatively specific for its acetyl-CoA substrate, utilizing propionyl-CoA and not acetyl-CoA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
P31660
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
P31660
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q56063
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q9KSC1
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q8NSH7, Q8NSL1
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q8X694
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q9XBT3
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q937N9
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q937N9
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q56063
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
physiological role of the methylcitric acid cycle is a catabolic reaction sequence concerning the oxidation of propionate into pyruvate, regulatory enzyme of this cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q8X694
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q9I5E3
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q8NSH7, Q8NSL1
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q56063
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q937N9
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q937N9
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Q8X694
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
-
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-methylcitrate + CoA
show the reaction diagram
-
reaction is followed by isomerization of (2S,3S)-2-methylcitrate, i.e. (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate, to (2R,3S)-2-methylisocitrate, i.e. (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate, via cis-2-methylaconitate, key enzyme in the methylcitrate cycle for alpha-oxidation of propionate to pyruvate, overview
-
ir
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Neurospora crassa IFO 6067, Mucor rouxianus IFO 5773
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas putida contig10768
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Candida pararugosa IFO 0966, Rhizopus microsporus var. chinensis IAM 6003
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Saccharomyces cerevisiae SANK 50182
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Ustilago utriculosa F-B-5
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli JM109
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas syringae contig5376, Shewanella putrefaciens contig93
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Cyberlindnera saturnus IAM 4094
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Burkholderia cepacia contig261, Pseudomonas fluorescens contig267
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli K12
-
first key enzyme of the methylcitric acid cycle, implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Pseudomonas aeruginosa PAO
-
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Saccharomyces cerevisiae CBS 8066
-
2-methylcitrate pathway is the major pathway of propionate metabolism
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Ustilago crus-galli F-B-6
-
-
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Burkholderia sacchari IPT101
-
first key enzyme of the methylcitric acid cycle, implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
Q8X694
first key enzyme of the methylcitric acid cycle
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
Q8X694
implicated in the metabolism of propionate
-
?
propanoyl-CoA + H2O + oxaloacetate
2-methylcitrate + CoA
show the reaction diagram
Escherichia coli O157:H17
Q8X694
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
propionyl-CoA + H2O + oxaloacetate
(2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
?
additional information
?
-
Q50I20
key enzyme of the methylcitrate cycle and required for fungal propionate degradation
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
1 mM, relative activity 104%
Fe2+
-
0.02 mM, relative activity 104%
Mg2+
-
1 mM, relative activity 102%
additional information
-
Mg2+, Cu2+, Ni2+, Co2+, and EDTA do not have any effect on the enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acetyl-CoA
competitive to CoA
ADP
-
slight inhibition
ATP
-
30% inhibition at 3 mM
Monoiodoacetamide
-
-
p-chloromercuribenzoate
-
-
Propionate
-
-
propionyl-CoA
competitive to CoA
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Isocitrate
-
1 mM, relative activity 101%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00126
acetyl-CoA
-
-
0.0025
acetyl-CoA
-
pH 8.0, 25C, purified native enzyme
0.0026
acetyl-CoA
pH 8.0, 25C, purified native enzyme
0.00271
acetyl-CoA
-
-
0.0029
acetyl-CoA
-
-
0.007
acetyl-CoA
-
-
0.101
acetyl-CoA
-
completed mutant strain W620
0.265
acetyl-CoA
in 50 mM HEPES pH 8.0, at 30C
0.35
acetyl-CoA
-
0.022
Butyryl-CoA
-
-
0.0085
CoA
pH 8.0, 25C, purified native enzyme, in absence of propionyl-CoA
0.0325
CoA
pH 8.0, 25C, purified native enzyme, in presence of propionyl-CoA
0.00063
oxaloacetate
-
pH 8.0, 25C, purified native enzyme
0.002
oxaloacetate
-
second substrate propionyl-CoA
0.00258
oxaloacetate
-
propionyl-CoA
0.0027
oxaloacetate
pH 8.0, 25C, purified native enzyme
0.00281
oxaloacetate
-
propionyl-CoA
0.003
oxaloacetate
-
-
0.003
oxaloacetate
-
-
0.00385
oxaloacetate
-
acetyl-CoA
0.005
oxaloacetate
-
completed mutant strain W620
0.00964
oxaloacetate
-
acetyl-CoA
0.013
oxaloacetate
using acetyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30C
0.015
oxaloacetate
using propanoyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30C
0.025
oxaloacetate
-
-
0.0017
propanoyl-CoA
-
pH 8.0, 25C, purified native enzyme
0.0019
propanoyl-CoA
pH 8.0, 25C, purified native enzyme
0.045
propanoyl-CoA
in 50 mM HEPES pH 8.0, at 30C
0.061
propanoyl-CoA
-
0.00188
propionyl-CoA
-
-
0.00194
propionyl-CoA
-
-
0.003
propionyl-CoA
-
-
0.0053
propionyl-CoA
-
-
0.016
propionyl-CoA
-
-
0.017
propionyl-CoA
-
-
0.037
propionyl-CoA
-
completed mutant strain W620
0.025
valeryl-CoA
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.87
acetyl-CoA
Salmonella enterica subsp. enterica serovar Typhimurium
Q56063
in 50 mM HEPES pH 8.0, at 30C
15.5
acetyl-CoA
Fusarium solani
-
-
29.9
acetyl-CoA
Fusarium verticillioides
-
-
23
oxaloacetate
Aspergillus nidulans
-
pH 8.0, 25C, purified native enzyme
8.25
propanoyl-CoA
Salmonella enterica subsp. enterica serovar Typhimurium
Q56063
in 50 mM HEPES pH 8.0, at 30C
23
propanoyl-CoA
Aspergillus nidulans
-
pH 8.0, 25C, purified native enzyme
12.5
propionyl-CoA
Fusarium verticillioides
-
-
15.9
propionyl-CoA
Fusarium solani
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
7.05
acetyl-CoA
Salmonella enterica subsp. enterica serovar Typhimurium
Q56063
in 50 mM HEPES pH 8.0, at 30C
29
183
propanoyl-CoA
Salmonella enterica subsp. enterica serovar Typhimurium
Q56063
in 50 mM HEPES pH 8.0, at 30C
782
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.053
propionyl-CoA
versus CoA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.002
-
CBS 1904, CBS 2015
0.003
-
wild-type strain, carbon source glucose
0.004
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source glucose
0.005
-
OM-102
0.0051
-
nuclei-free homogenate, glucose grown cells
0.006
-
CBS 6174
0.007
-
IFO 0720
0.0092
-
nuclei-free homogenate, n-alkane grown cells
0.011
Mucor rouxianus
-
-
0.011
-
wild-type strain, carbon source olive oil
0.012
-
IFO 1659, propionyl-CoA
0.012
-
CBS 565
0.014
Cryptococcus curvatus, Ustilago utriculosa
-
-
0.019
-
heavy particulate fraction, glucose grown cells
0.025
-
-
0.028
-
IFO 1457
0.029
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source olive oil
0.03
-
heavy particulate fraction, n-alkane grown cells
0.03
-
IFO 1659
0.034
-
IFO 1542
0.042
-
CBS 0882
0.052
-
R-2, propionyl-CoA
0.053
Ustilago crus-galli
-
-
0.056
-
CBS 0586
0.085
-
wild-type strain, carbon source peptone
0.12
-
partially purified preparation
0.13
-
strain SMI45
0.165
-
complemented methylcitrate synthase mutant 11/12 B1, carbon source peptone
0.2
-
strain SMB/acuA
0.21
-
IFO 1659, acetyl-CoA
0.22
-
R-2, acetyl-CoA
0.436
-
substrate propionyl-CoA
0.623
-
substrate acetyl-CoA
14.5
-
purified native enzyme, with oxaloacetate and propionyl-CoA
15.31
-
propionyl-CoA
17.7
purified native enzyme, with oxaloacetate and propionyl-CoA
19.05
-
acetyl-CoA
19.55
-
propionyl-CoA
36.6
-
acetyl-CoA
41.5
-
purified native enzyme, with oxaloacetate and acetyl-CoA
48
purified native enzyme, with oxaloacetate and acetyl-CoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 10.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45 - 50
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
49 - 54
-
maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.82
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Salmonella enterica JE2170
-
-
-
Manually annotated by BRENDA team
-
enzyme possesses a mitochondrial signal import sequence
Manually annotated by BRENDA team
enzyme possesses a mitochondrial signal import sequence
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
42570
prpC1, amino acid sequence calculated from cDNA
488176
42600
prpC2, amino acid sequence calculated from cDNA
488176
42690
-
amino acid sequence calculated from cDNA
488174
42720
-
amino acid sequence
488173
43000
-
SDS-PAGE
488171
43100
amino acid sequence calculated from cDNA
488175
49000
-
determined by SDS-PAGE
705576
90000
-
native enzyme, gel filtration
488089
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 45000, native enzyme, SDS-PAGE, x * 514100, sequence calculation
?
x * 43683, calculated from sequence
?
Cupriavidus necator H16
-
x * 43683, calculated from sequence
-
dimer
-
2 * 43000, native and recombinant enzyme, SDS-PAGE, 2 * 50580, sequence calculation
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
-
N-terminal processing of the mitochondrial signal import sequence
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
3 h, stable
661700
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
3 h, stable up to
661700
50
-
activity completely lost after 10 min
488166
52 - 60
the enzyme has a melting temperature of 52C. Tm increases to 60C when the enzyme is preincubated with 0.1 mM oxaloacetate
720336
60
half-life 11 min
661700
95
-
half-life at 90C is 12 min, active even at mesophilic temperatures, at 40C enzyme retains 20-30% of the maximum activity
488087
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme 136fold to homogeneity
native enzyme 72.5fold from strain SMB/acuA
-
on a gravity-flow Ni-NTA agarose column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
into the pCR2.1 and pUChph1 vectors
-
gene mscA, DNA and amino acid sequence determination and analysis, overexpression in Aspergillus nidulans
-
prpC cloned, sequenced and expressed in Escherichia coli
-
genome sequencing, prpDBC gene cluster, prpC2 gene for 2-methylcitrate synthase, cloned into vector pTrc99a and expressed in Escherichia coli
prpC encoded enzyme complements a prpC mutant of Salmonella enterica serovar Typhimurium, transduction of genomic DNA ligated into the cosmid pHC79 to Escherichia coli S17-1
-
expression in Escherichia coli
the chromosomal gene encoding the 2-methyl-cis-aconitate hydratase is disrupted by direct insertion of a copy of an additional 2-methylcitrate synthase gene (prpc). 2-Methylcitrate synthase is expressed under the control of the constitutive kanamycin-resistance gene resulting in up to 20fold higher specific 2-methylcitrate synthase activities in comparison to the wild-type. The disruption of the acnM gene by insertion of prpC leads to a propionate- and levulinate-negative phenotype of the engineered strains and accumulation of 2-methylcitrate in the medium
-
gene prpC sequenced
-
gene prpC sequenced; pRES1, prpC gene cloned into mutant Escherichia coli W620
-
into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells
-
gene sequenced
-
gene prpC sequenced
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
construction of an enzyme deletion mutant strain, which is unable to grow on propionate as sole carbon source, phenotype and growth behaviour analysis, accumulation of propionyl-CoA, morphology of conidia, deletion leads to an attenuation of virulence, overview
additional information
-
construction of an enzyme deletion mutant strain, which is unable to grow on propionate, phenotype and growth behaviour, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
invasive aspergillosis is a life-threatening disease mainly caused by the fungus Aspergillus fumigatus, blocking of methylcitrate synthase abrogates fungal growth and provides a suitable target for new antifungals
agriculture
-
implications for propionate as an antifungal agent