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EC Tree
IUBMB Comments This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
citrate synthetase, mitochondrial citrate synthase, peroxisomal citrate synthase, si-citrate synthase, type ii citrate synthase, citrate condensing enzyme, citrate synthase cit1, bifunctional citrate synthase/2-methylcitrate synthase,
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citrate condensing enzyme
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citrate oxaloacetate-lyase (CoA-acetylating)
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citrate oxaloacetate-lyase, CoA-acetylating
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citrate synthase Cit1
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citrate synthetase
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citric-condensing enzyme
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oxalacetic transacetase
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oxaloacetate transacetase
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synthase, citrate
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additional information
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enzyme participates in multienzyme complexes of enzymes belonging to tricarboxylic acid cycle, probably substrate channeling, in vivo NMR measurements
CIT1
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CIT1
CIT1 gene encodes a specific citrate synthase
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acetyl-CoA + H2O + oxaloacetate = citrate + CoA
free diffusion mechanism, no channeling of oxaloacetate from malate dehydrogenase
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-, -, -, -, -, -, -, -, -, -, -, -, -
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acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
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acetyl-CoA + H2O + oxaloacetate
citrate + CoA
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
additional information
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay
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ir
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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regulation of mitochondrial uptake and efflux by citrate synthase activity
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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entry step to tricarboxylic acid cycle
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?
additional information
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no activity with propionyl-CoA
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additional information
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cells with cit1 deletion show higher tendency toward glutathione depletion and subsequent accumulation of reactive oxygen species than the wild type. GSH deficiency in cit1 null cells is caused by an insufficient supply of glutamate necessary for biosynthesis of GSH rather than the depletion of reducing power required for reduction of GSSG to GSH
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additional information
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yeast mutants lacking mitochondrial NAD+-specific isocitrate dehydrogenase or aconitase share several growth phenotypes as well as patterns of specific protein expression that differ from the parental strain. These shared properties of idhD and aco1D strains are eliminated or moderated by codisruption of the CIT1 gene encoding mitochondrial citrate synthase
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
additional information
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acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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regulation of mitochondrial uptake and efflux by citrate synthase activity
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?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
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entry step to tricarboxylic acid cycle
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?
additional information
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cells with cit1 deletion show higher tendency toward glutathione depletion and subsequent accumulation of reactive oxygen species than the wild type. GSH deficiency in cit1 null cells is caused by an insufficient supply of glutamate necessary for biosynthesis of GSH rather than the depletion of reducing power required for reduction of GSSG to GSH
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?
additional information
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yeast mutants lacking mitochondrial NAD+-specific isocitrate dehydrogenase or aconitase share several growth phenotypes as well as patterns of specific protein expression that differ from the parental strain. These shared properties of idhD and aco1D strains are eliminated or moderated by codisruption of the CIT1 gene encoding mitochondrial citrate synthase
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NADH
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51% inhibition of peroxisomal isozyme, 12% inhibition of mitochondrial isozyme at 5 mM
NADP+
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only CS II slightly
NADPH
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40% inhibition by 5 mM, both isoenzymes
additional information
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no inhibition by NAD+
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0.076
acetyl-CoA
pH 7.5, 37°C
0.003 - 0.007
oxaloacetate
0.004
acetyl-CoA
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mitochondrial isozyme CS I
0.011
acetyl-CoA
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native peroxisomal isozyme CS II
0.013 - 0.014
acetyl-CoA
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recombinant peroxisomal isozyme CS II
0.003
oxaloacetate
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mitochondrial isozyme CS I
0.006 - 0.007
oxaloacetate
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native and recombinant peroxisomal isozyme CS II
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11.2
acetyl-CoA
pH 7.5, 37°C
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213
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purified fusion protein of citrate synthase and mitochondrial malate dehydrogenase, coupled assay
70
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purified recombinant isozyme CS II
additional information
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8
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both mitochondrial and peroxisomal enzymes
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SwissProt
brenda
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45% of CS II is bound to mitochondrial inner membrane and 2% of CS I
brenda
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brenda
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mitochondrial isozyme
brenda
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citrate uptake into mitochondria
brenda
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brenda
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peroxisomal isozyme
brenda
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50000
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2 * 50000, CS II, SDS-PAGE
additional information
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enzyme participates in multienzyme complexes of enzymes belonging to tricarbonic acid cycle, probably substrate channeling, in vivo NMR measurements
100000
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gel filtration
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dimer
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2 * 50000, CS II, SDS-PAGE
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additional information
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no channeling of oxaloacetate between malate dehydrogenase and citrate synthase in a recombinant fusion protein using a coupled assay
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peroxisomal enzyme protected by oxaloacetate
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-20°C, Tris-HCl buffer, pH 7.4, 50% glycerol, 1 month
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recombinant His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase from E. coli
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recombinant peroxisomal isozyme CS II from E. coli
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expression in Escherichia coli
cloning of His-tagged fusion protein of citrate synthase and mitochondrial malate dehydrogenase, expression in Escherichia coli
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expression of labeled mitochondrial isozyme in Saccharomyces from plasmid, NMR measurements in vivo
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functional expression of peroxisomal isozyme CS II in citrate synthase deficient Escherichia coli mutant and wild-type Escherichia coli strain
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Sandor, A.; Johnson, J.H.; Srere, P.A.
Cooperation between enzyme and transporter in the inner mitochondrial membrane of yeast
J. Biol. Chem.
269
29609-29612
1994
Saccharomyces cerevisiae
brenda
Kispal, G.; Srere, P.A.
Studies on yeast peroxisomal citrate synthase
Arch. Biochem. Biophys.
286
132-137
1991
Saccharomyces cerevisiae
brenda
Haggie, P.M.; Brindle, K.M.
Mitochondrial citrate synthase is immobilized in vivo
J. Biol. Chem.
274
3941-3945
1999
Saccharomyces cerevisiae
brenda
Pettersson, H.; Olsson, P.; Bulow, L.; Pettersson, G.
Kinetics of the coupled reaction catalyzed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase
Eur. J. Biochem.
267
5041-5046
2000
Saccharomyces cerevisiae
brenda
Ruprich-Robert, G.; Zickler, D.; Berteaux-Lecellier, V.; Velot, C.; Picard, M.
Lack of mitochondrial citrate synthase discloses a new meiotic checkpoint in a strict aerobe
EMBO J.
21
6440-6451
2002
Saccharomyces cerevisiae, Podospora anserina (Q9HFI1), Podospora anserina
brenda
Graybill, E.R.; Rouhier, M.F.; Kirby, C.E.; Hawes, J.W.
Functional comparison of citrate synthase isoforms from S. cerevisiae
Arch. Biochem. Biophys.
465
26-37
2007
Saccharomyces cerevisiae (P00890)
brenda
Lin, A.P.; Hakala, K.W.; Weintraub, S.T.; McAlister-Henn, L.
Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase
Arch. Biochem. Biophys.
474
205-212
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae MMY011
brenda
Qiu, Z.; MacRae, T.H.
ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults
FEBS J.
275
3556-3566
2008
Saccharomyces cerevisiae
brenda