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Information on EC 2.3.3.16 - citrate synthase (unknown stereospecificity) and Organism(s) Daucus carota and UniProt Accession O80433

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EC Tree
IUBMB Comments
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
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This record set is specific for:
Daucus carota
UNIPROT: O80433
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Word Map
The taxonomic range for the selected organisms is: Daucus carota
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
citrate synthetase, mitochondrial citrate synthase, peroxisomal citrate synthase, si-citrate synthase, type ii citrate synthase, citrate condensing enzyme, citrate synthase cit1, bifunctional citrate synthase/2-methylcitrate synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
citrate condensing enzyme
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citrate oxaloacetate-lyase (CoA-acetylating)
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citrate oxaloacetate-lyase, CoA-acetylating
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citrate synthase
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citrate synthetase
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citric synthase
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citric-condensing enzyme
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citrogenase
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oxalacetic transacetase
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oxaloacetate transacetase
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synthase, citrate
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:oxaloacetate C-acetyltransferase (thioester-hydrolysing)
This entry has been included to accommodate those citrate synthases for which the stereospecificity with respect to C-2 of oxaloacetate has not been established [cf. EC 2.3.3.1, citrate (Si)-synthase and EC 2.3.3.3, citrate (Re)-synthase].
CAS REGISTRY NUMBER
COMMENTARY hide
9027-96-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
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-
?
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + oxaloacetate + H2O
citrate + CoA
show the reaction diagram
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-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.78
transgenic Arabidopsis thaliana plants overexpressing the Daucus carota citrate synthase
0.00078
recombinant in transgenic Arabidopsis thaliana plants
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CISY_DAUCA
472
0
52657
Swiss-Prot
Mitochondrion (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ectopic overexpression in Arabidopsis thaliana strain WS via infection with Agrobacterium tumefaciens, DNA sequence determination
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
overexpression of Daucus carota enzyme in Arabidopsis thaliana plant mitochondria improves the ability of the plants to grow in phosphorus limited soil due to enhanced citrate excretion from the roots
agriculture
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Koyama, H.; Kawamura, A.; Kihara, T.; Hara, T.; Takita, E.; Shibata, D.
Overexpression of mitochondrial citrate synthase in Arabidopsis thaliana improved growth on a phosphorus-limited soil
Plant Cell Physiol.
41
1030-1037
2000
Arabidopsis thaliana, Arabidopsis thaliana WS, Daucus carota, Daucus carota (O80433)
Manually annotated by BRENDA team