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Information on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase and Organism(s) Blattella germanica and UniProt Accession P54961

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This record set is specific for:
Blattella germanica
UNIPROT: P54961 not found.
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The taxonomic range for the selected organisms is: Blattella germanica
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hmg-coa, hmg-coa synthase, hmgcs2, hmgs, hmgcs1, 3-hydroxy-3-methylglutaryl-coa synthase, hydroxymethylglutaryl-coa synthase, 3-hydroxy-3-methylglutaryl-coenzyme a synthase, 3-hydroxy-3-methylglutaryl coenzyme a synthase, hmgcs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating)
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3-hydroxy-3-methylglutaryl CoA synthetase
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3-hydroxy-3-methylglutaryl coenzyme A synthase
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3-hydroxy-3-methylglutaryl coenzyme A synthetase
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3-hydroxy-3-methylglutaryl-CoA synthase
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3-hydroxy-3-methylglutaryl-coenzyme A synthase
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acetoacetyl coenzyme A transacetase
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beta-hydroxy-beta-methylglutaryl-CoA synthase
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HMG-CoA synthase
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hydroxymethylglutaryl CoA synthetase
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hydroxymethylglutaryl coenzyme A synthase
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hydroxymethylglutaryl coenzyme A-condensing enzyme
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hydroxymethylglutaryl-coenzyme A synthase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
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hydrolysis
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Acyl group transfer
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transacetylation
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:acetoacetyl-CoA C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-44-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetoacetyl-CoA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015 - 0.0152
acetyl-CoA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00126
acetoacetyl-CoA
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cockroach, cytosolic isoform 1
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HMCS1_BLAGE
453
0
50332
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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SDS-PAGE, immunoblot by antibodies, prediction from cDNA nucleotide sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 cytosolic HMG-CoA synthase genes, HMGS-1 and HMGs-2 described, HMGS-1 cloned in expression vector pSBLA1 and overexpressed in Escherichia coli BL21DE3 pLys S
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intronless gene, encodes for an active enzyme able to complement eukaryotic Mev-1 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Misra, I.; Charlier, H.A.; Miziorko, H.M.
Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Evaluation of the role of cysteines in reaction chemistry
Biochim. Biophys. Acta
1247
253-259
1995
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus, Cricetulus griseus (P13704), Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Misra, I.; Miziorko, H.M.
Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase H264 with acetoacetyl-CoA
Biochemistry
35
9610-9616
1996
Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus, Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Cabano, J.; Buesa, C.; Hegardt, F.G.; Marrero, P.F.
Catalytic properties of recombinant 3-hydroxy-3-methylglutaryl coenzyme A synthase-1 from Blattella germanica
Insect Biochem. Mol. Biol.
27
499-505
1997
Blattella germanica, Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Diploptera punctata, Locusta migratoria
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Miziorko, H.M.
3-Hydroxy-3-methylglutaryl-CoA synthase: Participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate
Biochemistry
39
14670-14681
2000
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P54839), Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Homo sapiens (Q01581), Mesocricetus auratus, Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Cricetulus griseus (P13704), Rattus norvegicus (P17425), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Caenorhabditis elegans (P54871), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Zajicek, J.; Miziorko, H.M.
3-hydroxy-3-methylglutaryl-CoA synthase. A role for glutamate 95 in general acid/base catalysis of C-C bond formation
J. Biol. Chem.
275
17946-17953
2000
Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Casals, N.; Buesa, C.; Marrero, P.F.; Belles, X.; Hegardt, F.G.
3-Hydroxy-3-methylglutaryl coenzyme A synthase-1 of Blattella germanica has structural and functional features of an active retrogene
Insect Biochem. Mol. Biol.
31
425-433
2001
Blattella germanica
Manually annotated by BRENDA team
Sutherlin, A.; Hedl, M.; Sanchez-Neri, B.; Burgner, J.W.; Stauffacher, C.V.; Rodwell, V.W.
Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis
J. Bacteriol.
184
4065-4070
2002
Blattella germanica, Borreliella burgdorferi, Bos taurus, Streptomyces sp., Gallus gallus, Streptococcus pneumoniae, Enterococcus faecalis, Homo sapiens, Mesocricetus auratus, Staphylococcus aureus, Mus musculus, Rattus norvegicus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Streptococcus pyogenes, Sus scrofa
Manually annotated by BRENDA team