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Information on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase and Organism(s) Rattus norvegicus and UniProt Accession P17425

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Rattus norvegicus
UNIPROT: P17425 not found.
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
hmg-coa, hmg-coa synthase, hmgcs2, hmgs, hmgcs1, 3-hydroxy-3-methylglutaryl-coa synthase, hydroxymethylglutaryl-coa synthase, 3-hydroxy-3-methylglutaryl-coenzyme a synthase, 3-hydroxy-3-methylglutaryl coenzyme a synthase, hmgcs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxy-3-methylglutaryl-coenzyme A synthase
-
HMG-CoA synthase
-
(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating)
-
-
-
-
3-hydroxy-3-methylglutaryl CoA synthetase
-
-
-
-
3-hydroxy-3-methylglutaryl coenzyme A synthase
3-hydroxy-3-methylglutaryl coenzyme A synthetase
-
-
-
-
3-hydroxy-3-methylglutaryl-CoA synthase
3-hydroxy-3-methylglutaryl-coenzyme A synthase
acetoacetyl coenzyme A transacetase
-
-
-
-
beta-hydroxy-beta-methylglutaryl-CoA synthase
-
-
-
-
HMG-CoA synthase
hydroxymethylglutaryl CoA synthase 2
-
hydroxymethylglutaryl CoA synthetase
-
-
-
-
hydroxymethylglutaryl coenzyme A synthase
-
-
-
-
hydroxymethylglutaryl coenzyme A-condensing enzyme
-
-
-
-
hydroxymethylglutaryl-CoA synthase
-
hydroxymethylglutaryl-coenzyme A synthase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
-
-
-
-
hydrolysis
-
-
-
-
Acyl group transfer
transacetylation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:acetoacetyl-CoA C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9027-44-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
-
-
-
?
additional information
?
-
-
enzyme activity and mRNA levels are upregulated after 3-thia fatty acid treatment
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E,E)-11-[3-(hydroxymethyl)-4-oxo-2-oxytanyl]-3,5,7-trimethyl-2,4-undecadienenoic acid
-
-
acetoacetyl-CoA
hymeglusin
-
-
L-659,699
MgCl2
-
mitochondrial enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009 - 0.811
acetyl-CoA
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
satellite glial cells in dorsal root ganglia. HMGCS1 but not HMGCS2 immunoreactivity in satellite glial cells is reduced in the fifth lumbar (L5) dorsal root ganglion that contain axotomized neurons following L5 spinal nerve ligation in rats. HMGCS1 protein level in axotomized L5 dorsal root ganglia is reduced after spinal nerve ligation to 66% at 3 days and 58% at 28 days of its level in control samples, whereas HMGCS2 protein is comparable between injured and control dorsal root ganglia
Manually annotated by BRENDA team
satellite glial cells in dorsal root ganglia. HMGCS1 but not HMGCS2 immunoreactivity in satellite glial cells is reduced in the fifth lumbar (L5) dorsal root ganglion that contain axotomized neurons following L5 spinal nerve ligation in rats. HMGCS1 protein level in axotomized L5 dorsal root ganglia is reduced after spinal nerve ligation to 66% at 3 days and 58% at 28 days of its level in control samples, whereas HMGCS2 protein is comparable between injured and control dorsal root ganglia
Manually annotated by BRENDA team
-
mRNA levels in newborn rats is low, when suckling begins, there is a net increase in gene expression up to day 12, levels then plateau until day 15, and then decreases progressively throughout the suckling period, becoming zero after weaning (day 21) and remains so in the adult rat
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HMCS1_RAT
520
0
57434
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57370
-
deduced amino acid sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned and sequenced
-
rat mitochondrial HMG-CoA synthase encodes an active enzyme in the eukaryotic cell line Mev-1 in Escherichia coli BL21 (DE3)
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
HMGCS1 expression is downregulated in dorsal root ganglia after spinal nerve injury
lower renal expression of HMGCS2 in SHRSPs than in SHRs or clofibrate-treated SHRSPs
the enzyme activity is higher in alcohol-fed rats, but the relative increase in enzyme activity in alcohol-fed rats is less than the relative increase in enzyme expression
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Allred, J.B.
Properties and subcellular distribution of enzymes required for acetoacetate biosynthesis in chicken liver
Biochim. Biophys. Acta
297
22-30
1973
Cavia porcellus, Gallus gallus, Rattus norvegicus
Manually annotated by BRENDA team
Cornforth, J.W.; Phillips, G.T.
Substrate stereochemistry of 3-hydroxy-3-methylglutaryl-coenzyme A synthase
Eur. J. Biochem.
42
591-604
1974
Saccharomyces cerevisiae, Rattus norvegicus
Manually annotated by BRENDA team
Clinkenbeard, K.D.; Sugiyama, T.; Reed, W.D.; Lane, M.D.
Cyoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase from liver
J. Biol. Chem.
250
3124-3135
1975
Saccharomyces cerevisiae, Gallus gallus, Columba livia, Meleagris gallopavo, Rattus norvegicus
-
Manually annotated by BRENDA team
Reed, W.D.; Clinkenbeard, K.D.; Lane, M.D.
Molecular and catalytic properties of mitochondrial (ketogenic) 3-hydroxy-3-methylglutaryl coenzyme A synthase of liver
J. Biol. Chem.
250
3117-3123
1975
Gallus gallus, Rattus norvegicus
Manually annotated by BRENDA team
Menahan, L.A.; Hron, W.T.; Hinkelman, D.G.; Miziorko, H.M.
Interrelationships between 3-hydroxy-3-methylglutaryl-CoA synthase, aetoacetyl-CoA and ketogenesis
Eur. J. Biochem.
119
287-296
1981
Gallus gallus, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Manually annotated by BRENDA team
Greenspan, M.D.; Yudkovitz, J.B.; Lo, C.Y.L.; Chen, J.S.; Alberts, A.W.; Hunt, V.M.; Chang, M.N.; Yang, S.S.; Thompson, K.L.; Chiang, Y.C.P.; Chabala, J.C.; Monaghan, R.L.; Schwartz, R.L.
Inhibition of hydroxymethylglutaryl-coenzyme A synthase by L659,699
Proc. Natl. Acad. Sci. USA
84
7488-7492
1987
Gallus gallus, Cricetulus griseus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Mayer, R.J.; Louis-Flamberg, P.; Elliott, J.D.; Fisher, M.; Leber, J.
Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A synthase by antibiotic 1233A and other beta-lactones
Biochem. Biophys. Res. Commun.
169
610-616
1990
Rattus norvegicus
Manually annotated by BRENDA team
Greenspan, M.D.; Bull, H.B.; Yudkovitz, J.B.; Hanf, D.P.; Alberts, A.W.
Inhibition of 3-hydroxy-3-methylglutaryl-CoA synthase and cholesterol biosynthesis by beta-lactone inhibitors and binding of these inhibitors to the enzyme
Biochem. J.
289
889-895
1993
Mesocricetus auratus, Mus musculus, Rattus norvegicus
-
Manually annotated by BRENDA team
Misra, I.; Narasimhan, C.; Miziorko, H.M.
Avian 3-hydroxy-3-methylglutaryl-CoA synthase
J. Biol. Chem.
268
12129-12135
1993
Gallus gallus, Rattus norvegicus
Manually annotated by BRENDA team
Rokosz, L.L.; Boulton, D.A.; Butkiewicz, E.A.; Sanyal, G.; Cueto, M.A.; Lachance, P.A.; Hermes, J.D.
Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: expression, purification, andcharacterization of recombinant wild-type and cys-129 mutant enzymes
Arch. Biochem. Biophys.
312
1-13
1994
Gallus gallus, Cricetulus griseus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Misra, I.; Charlier, H.A.; Miziorko, H.M.
Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Evaluation of the role of cysteines in reaction chemistry
Biochim. Biophys. Acta
1247
253-259
1995
Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus, Cricetulus griseus (P13704), Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Montamat, F.; Guilloton, M.; Karst, F.; Delrot, S.
Isolation and characterization of a cDNA encoding Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coenzyme A synthase
Gene
167
197-201
1995
Arabidopsis thaliana, Catharanthus roseus, Gallus gallus, Cricetulus griseus, Homo sapiens, Raphanus sativus, Rattus norvegicus
Manually annotated by BRENDA team
Scharnagl, H.; Mrz, W.; Schliack, M.; Lser, R.; Gross, W.
A novel assay of cytosolic 3-hydroxy-3-methylglutaryl-coenzyme A synthase activity using reversed-phase ion-pair chromatography: demonstration that Lifibrol (K12.148) modulates the enzyme activity
J. Lipid Res.
36
622-627
1995
Bos taurus, Gallus gallus, Cricetulus griseus, Homo sapiens, Mesocricetus auratus, Rattus norvegicus
Manually annotated by BRENDA team
Misra, I.; Miziorko, H.M.
Evidence for the interaction of avian 3-hydroxy-3-methylglutaryl-CoA synthase H264 with acetoacetyl-CoA
Biochemistry
35
9610-9616
1996
Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus, Blattella germanica (P54870), Blattella germanica (P54961)
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Miziorko, H.M.
3-Hydroxy-3-methylglutaryl-CoA synthase: Participation of invariant acidic residues in formation of the acetyl-S-enzyme reaction intermediate
Biochemistry
39
14670-14681
2000
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P54839), Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Homo sapiens (Q01581), Mesocricetus auratus, Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Cricetulus griseus (P13704), Rattus norvegicus (P17425), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Caenorhabditis elegans (P54871), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Chun, K.Y.; Vinarov, D.A.; Zajicek, J.; Miziorko, H.M.
3-hydroxy-3-methylglutaryl-CoA synthase. A role for glutamate 95 in general acid/base catalysis of C-C bond formation
J. Biol. Chem.
275
17946-17953
2000
Gallus gallus, Gallus gallus (P23228), Homo sapiens, Homo sapiens (P54868), Methanothermobacter thermautotrophicus, Sus scrofa (O02734), Borreliella burgdorferi (O51626), Rattus norvegicus (P22791), Mus musculus (P54869), Blattella germanica (P54870), Blattella germanica (P54961), Arabidopsis thaliana (P54873), Schizosaccharomyces pombe (P54874), Pinus sylvestris (P93773)
Manually annotated by BRENDA team
Barrero, M.J.; Alho, C.S.; Ortiz, J.A.; Hegardt, F.G.; Haro, D.; Marrero, P.F.
Low activity of mitochondrial HMG-CoA synthase in liver of starved pigletsis due to low levels of protein despite high mRNA levels
Arch. Biochem. Biophys.
385
364-371
2001
Rattus norvegicus, Rattus norvegicus Sprague-Dawley, Sus scrofa
Manually annotated by BRENDA team
Sutherlin, A.; Hedl, M.; Sanchez-Neri, B.; Burgner, J.W.; Stauffacher, C.V.; Rodwell, V.W.
Enterococcus faecalis 3-hydroxy-3-methylglutaryl coenzyme A synthase, an enzyme of isopentenyl diphosphate biosynthesis
J. Bacteriol.
184
4065-4070
2002
Blattella germanica, Borreliella burgdorferi, Bos taurus, Streptomyces sp., Gallus gallus, Streptococcus pneumoniae, Enterococcus faecalis, Homo sapiens, Mesocricetus auratus, Staphylococcus aureus, Mus musculus, Rattus norvegicus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Streptococcus pyogenes, Sus scrofa
Manually annotated by BRENDA team
Hegardt, F.G.
Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase: a control enzyme in ketogenesis
Biochem. J.
338
569-582
1999
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Madsen, L.; Garras, A.; Asins, G.; Serra, D.; Hegardt, F.G.; Berge, R.K.
Mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A synthase and carnitine palmitoyltransferase II as potential control sites for ketogenesis during mitochondrion and peroxisome proliferation
Biochem. Pharmacol.
57
1011-1019
1999
Rattus norvegicus
Manually annotated by BRENDA team
Tomoda, H.; Ohbayashi, N.; Morikawa, Y.; Kumagai, H.; Omura, S.
Binding site for fungal beta-lactone hymeglusin on cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase
Biochim. Biophys. Acta
1636
22-28
2004
Cricetinae, Rattus norvegicus
Manually annotated by BRENDA team
Yi, W.; Fu, P.; Fan, Z.; Aso, H.; Tian, C.; Meng, Y.; Liu, J.; Yamori, Y.; Nara, Y.; Ying, C.
Mitochondrial HMG-CoA synthase partially contributes to antioxidant protection in the kidney of stroke-prone spontaneously hypertensive rats
Nutrition
26
1176-1180
2010
Rattus norvegicus (P22791)
Manually annotated by BRENDA team
Satoh, M.; Haruta-Satoh, E.; Yamada, M.; Kado, S.; Nomura, F.
Overexpression of hydroxymethylglutaryl CoA synthase 2 and 2,4-dienoyl-CoA reductase in rat pancreas following chronic alcohol consumption
Pancreas
42
475-482
2013
Rattus norvegicus (P22791)
Manually annotated by BRENDA team
Liao, P.; Wang, H.; Hemmerlin, A.; Nagegowda, D.A.; Bach, T.J.; Wang, M.; Chye, M.L.
Past achievements, current status and future perspectives of studies on 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in the mevalonate (MVA) pathway
Plant Cell Rep.
33
1005-1022
2014
Arabidopsis thaliana (P54873), Brassica juncea (Q9M6U3), Camptotheca acuminata (B3GDB1), Catharanthus roseus, Gallus gallus (P23228), Hevea brasiliensis (Q94ET0), Hevea brasiliensis, Homo sapiens (A0A024R059), Melampsora larici-populina, Mesocricetus auratus, Pinus sylvestris (P93773), Populus trichocarpa x Populus deltoides, Rattus norvegicus (P17425), Salvia miltiorrhiza (D2DS62), Solanum lycopersicum (A9LRT6), Sus scrofa (O02734), Taxus x media (Q693N8)
Manually annotated by BRENDA team
Wang, F.; Xiang, H.; Fischer, G.; Liu, Z.; Dupont, M.J.; Hogan, Q.H.; Yu, H.
HMG-CoA synthase isoenzymes 1 and 2 localize to satellite glial cells in dorsal root ganglia and are differentially regulated by peripheral nerve injury
Brain Res.
1652
62-70
2016
Rattus norvegicus (P17425), Rattus norvegicus (P22791)
Manually annotated by BRENDA team