Information on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.3.3.10
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RECOMMENDED NAME
GeneOntology No.
hydroxymethylglutaryl-CoA synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
condensation
hydrolysis
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transacetylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ferrichrome A biosynthesis
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isoprene biosynthesis II (engineered)
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ketogenesis
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mevalonate pathway I
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mevalonate pathway II (archaea)
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mevalonate pathway III (archaea)
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mevalonate metabolism
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Synthesis and degradation of ketone bodies
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Valine, leucine and isoleucine degradation
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Butanoate metabolism
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Terpenoid backbone biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:acetoacetyl-CoA C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-44-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
bat
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Automatic Mining of ENzyme DAta
BjHMGS1
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Manually annotated by BRENDA team
BjHMGS2
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Manually annotated by BRENDA team
BjHMGS3
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Manually annotated by BRENDA team
BjHMGS4
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
pigeon
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
dogfish
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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SwissProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
turkey
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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no activity in Streptomyces albus
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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protozoa
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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1098308 A+, 1106012 A+, 1106014 A+, 1338253 A+, 1348945 A+, 1365513 A+, 1433840 A+, 1434629 A+, 1674487 A+, 1696700 A+, 1697241 A+, 1697306 A+, 1697907 A+, 1697946 A+, 1728265 A+, 1732219 A+, 1871199 A+, 1895863 A+, 1895867 A+, 1895877 A+, 1895926 A+, 1895930 A+, 1895962 A+, 1895979 A+, 2240790 A+, 2299560 A+, 2454605 A+, 2552809 A+, 2660594 A+, 2760352 A+, 2777725 A+, 286575 A+, 286584 A+, 2918330 A+, 2918344 A+, 2918376 A+, 2918384 A+, 2918386 A+, 488120 A+, 690593 A+, 726092 A+, 736978 A+, 785905 A+, 996360 A+, 996456 A+, 996501 A+, 996619 A+, 996623 A+, 996814 A+, 996866 A+, 1098573 A++, 1105974 A++, 1105977 A++, 1105985 A++, 1105988 A++, 1105989 A++, 1105991 A++, 1105993 A++, 1105996 A++, 1105997 A++, 1105998 A++, 1106008 A++, 1204795 A++, 1332753 A++, 1338250 A++, 1696685 A++, 1696720 A++, 1696930 A++, 1884197 A++, 1884211 A++, 1884220 A++, 1884252 A++, 1895889 A++, 1895890 A++, 1895891 A++, 1895906 A++, 2138550 A++, 2299526 A++, 2299527 A++, 2299538 A++, 2299539 A++, 2299545 A++, 2918357 A++, 487697 A++, 488106 A++, 658185 A++, 80976 A++, 1106002 A++, 1201978 A++, 1365504 A++, 1532295 A++, 1619770 A++, 1890989 A++, 1895861 A++, 1895869 A++, 1895874 A++, 1895909 A++, 1895910 A++, 2299529 A++, 2299532 A++, 2299533 A++, 2299536 A++, 2299542 A++, 2299543 A++, 2299544 A++, 2299551 A++, 2468749 A++, 2760498 A++, 488111 A++, 657877 A++, 688660 A++, 983286 A++, 996385 A++, 706004 A+++, 1105970 A++++, 1105975 A++++, 1105976 A++++, 1105978 A++++, 1105981 A++++, 1105995 A++++, 1106007 A++++, 996453 A++++
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Automatic Mining of ENzyme DAta
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
squirrel
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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UniProt
Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
trout
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Vitis vinifera x Vitis vinifera
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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3-hydroxy-3-methylglutaryl-CoA synthase is the second enzyme in the cytosolic mevalonate pathway
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-oxobutyryl-CoA + acetoacetyl-CoA + H2O
3-hydroxy-3-methyl-5-oxoheptanedioyl-CoA + CoA
show the reaction diagram
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?
acetyl-3'-dephospho-CoA + acetoacetyl-CoA + H2O
3-hydroxy-3-methylglutaryl-CoA + 3'-dephospho-CoA
show the reaction diagram
acetyl-CoA + 3'-dephospho-CoA + H2O
acetyldephospho-CoA + CoA
show the reaction diagram
acetyl-CoA + acetoacetyl-ACP + H2O
3-hydroxy-3-methylglutaryl-ACP
show the reaction diagram
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?
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + cysteamine + H2O
? + CoA
show the reaction diagram
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?
acetyl-CoA + H2O
acetate + CoA
show the reaction diagram
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + N-acetyl-S-acetoacetylcysteamine + H2O
? + CoA
show the reaction diagram
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?
acetylglutathione + acetoacetyl-CoA + H2O
?
show the reaction diagram
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?
acetylpantetheine + acetoacetyl-CoA + H2O
3-hydroxy-3-methylglutaryl-CoA + pantetheine
show the reaction diagram
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + acetoacetyl-CoA + H2O
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
acetyl-CoA + H2O + acetoacetyl-CoA
(S)-3-hydroxy-3-methylglutaryl-CoA + CoA
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
optimal concentration
Mn2+
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increases acetoacetate synthesis activity
additional information
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no stimulation by Mg2+, Mn2+, Zn2+ and Co2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E,E)-11-(3-hydroxymethyl-4-oxo-2-oxytanyl)-3,5,7-trimethyl-2,4-undecadienoic acid
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i.e. F-244
(E,E)-11-[3-(hydroxymethyl)-4-oxo-2-oxytanyl]-3,5,7-trimethyl-2,4-undecadienenoic acid
(S)-3-hydroxy-3-methylglutaryl-CoA
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1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
2-benzyl-9-(3,6-dioxocyclohexa-1,4-dienyl)-2,3,7,8-tetrahydro-6H-pyrido[1,2-a]pyrazine-1,4-dione
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3,3'-dimethylglutaryl-CoA
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3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA
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3-carboxypropionyl-CoA
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3-Chloropropionyl-CoA
3-hydroxy-3-methylglutaryl-CoA
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3-oxohexanoyl-CoA
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5,5'-dithiobis(2-nitrobenzoate)
acetoacetyl-CoA
acetyloxy(phenyl)mercury
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phenyl mercuric acetate
arsenite
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in presence of CoA, residual activity 25%
Bromoacetyl-CoA
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CdSO4
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residual activity 9%
diethylcarbamothioylsulfanyl N,N-diethylcarbamodithioate
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disulfiram
DL-3-hydroxy-3-methylglutaryl-CoA
DL-3-methylglutaryl-CoA
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hymeglusin
iodoacetamide
L-659,699
Lifibrol
Mg2+
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mitochondrial enzyme
MgCl2
N-ethylmaleimide
NaAsO2
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residual activity 90%
p-chloromercuribenzoate
palmitoyl-CoA
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mitochondrial synthase
phenyl arsenious oxide
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in presence of CoA, residual activity 41%; residual activity 75%
succinyl-CoA
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithioerythritol
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5 mM, activates
MgCl2
potassium phosphate
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Sodium phosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019 - 0.116
3-oxobutyl-CoA
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0.0001 - 0.115
acetoacetyl-CoA
0.0015 - 1.561
acetyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0017 - 4.6
acetyl-CoA