Information on EC 2.3.2.7 - aspartyltransferase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY
2.3.2.7
-
RECOMMENDED NAME
GeneOntology No.
aspartyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-asparagine + hydroxylamine = NH3 + beta-L-aspartylhydroxamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine:hydroxylamine gamma-aspartyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aspartotransferase
-
-
-
-
beta-aspartyl transferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37257-23-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
H37Ra, strain number 7417
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
asparagine + hydroxylamine
NH3 + beta-aspartohydroxamic acid
show the reaction diagram
-
strict specificity, L- and D-enantiomer equally effective, no substrate: L-glutamine
-
?
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
-
involved in initial step of asparagine metabolism
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
-
involved in initial step of asparagine metabolism
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
L-aspartate
-
20 mM, 30% inhibition
PCMB
-
reversible by GSH, 2-mercaptoethanol or 2,3-dimercaptopropanol, not L-cysteine
streptomycin
-
5 mM, 50% inhibition
L-cysteine
-
5 mM, 50% inhibition
additional information
-
no inhibition by NaN3, isonicotinic acid hydrazide, L-homoserine, L-glutamic acid, terminal amino acids of aspartic acid pathway, i.e. lysine, threonine, methionine and isoleucine, up to 20 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Isonicotinic acid hydrazide
-
activation
L-cystine
-
20 mM, slight activation
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
19.3
-
hydroxylamine
-
-
7.14
-
L-asparagine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.5
9.4
-
about half-maximal activity at pH 8.5 and 9.4
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
60
-
40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
-
-
6 h, 23% loss of activity
28
-
-
6 h, stable
37
-
-
6 h, about 8% loss of activity
60
-
-
10 min, about 45% loss of activity
65
-
-
10 min, about 66% loss of activity
70
-
-
t1/2: 1 min, about 55% loss of activity after 2 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
dialysis against 0.05 M Tris buffer, pH 7.0, 75% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE