Information on EC 2.3.2.7 - aspartyltransferase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY
2.3.2.7
-
RECOMMENDED NAME
GeneOntology No.
aspartyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-asparagine + hydroxylamine = NH3 + beta-L-aspartylhydroxamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine:hydroxylamine gamma-aspartyltransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aspartotransferase
-
-
-
-
beta-aspartyl transferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37257-23-1
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
H37Ra, strain number 7417
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
asparagine + hydroxylamine
NH3 + beta-aspartohydroxamic acid
show the reaction diagram
-
strict specificity, L- and D-enantiomer equally effective, no substrate: L-glutamine
-
?
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
-
involved in initial step of asparagine metabolism
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-asparagine + hydroxylamine
NH3 + beta-L-aspartohydroxamic acid
show the reaction diagram
-
involved in initial step of asparagine metabolism
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
no metal ion requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
L-aspartate
-
20 mM, 30% inhibition
PCMB
-
reversible by GSH, 2-mercaptoethanol or 2,3-dimercaptopropanol, not L-cysteine
streptomycin
-
5 mM, 50% inhibition
L-cysteine
-
5 mM, 50% inhibition
additional information
-
no inhibition by NaN3, isonicotinic acid hydrazide, L-homoserine, L-glutamic acid, terminal amino acids of aspartic acid pathway, i.e. lysine, threonine, methionine and isoleucine, up to 20 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Isonicotinic acid hydrazide
-
activation
L-cystine
-
20 mM, slight activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
19.3
hydroxylamine
-
-
7.14
L-asparagine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5 - 9.4
-
about half-maximal activity at pH 8.5 and 9.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40 - 60
-
40°C: about 50% of maximal activity, 60°C: about 80% of maximal activity
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0
-
6 h, 23% loss of activity
488009
28
-
6 h, stable
488009
37
-
6 h, about 8% loss of activity
488009
60
-
10 min, about 45% loss of activity
488009
65
-
10 min, about 66% loss of activity
488009
70
-
t1/2: 1 min, about 55% loss of activity after 2 min
488009
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against 0.05 M Tris buffer, pH 7.0, 75% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE