Information on EC 2.3.2.4 - gamma-glutamylcyclotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.3.2.4
-
RECOMMENDED NAME
GeneOntology No.
gamma-glutamylcyclotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(gamma-L-glutamyl)-L-amino acid = 5-oxoproline + L-amino acid
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminoacyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gamma-glutamyl cycle
-
-
glutathione metabolism
-
-
Glutathione metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
(gamma-L-glutamyl)-L-amino-acid gamma-glutamyltransferase (cyclizing)
The enzyme acts on derivatives of L-glutamate, L-2-aminobutanoate, L-alanine and glycine.
CAS REGISTRY NUMBER
COMMENTARY hide
9045-44-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
animal
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
house fly
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
tobacco, cv. Samsun
-
-
Manually annotated by BRENDA team
plant
-
-
-
Automatic Mining of ENzyme DAta
Holtzman
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme knockdown inhibits glioma cell T98G and U251 proliferation and colony formation
metabolism
-
isoform GGCT2;1 participates in the cellular response to arsenic via glutathione degradation. The enzyme ensures sufficient glutathione turnover during abiotic stress by recycling L-Glu. Enzyme overexpression provides enhanced arsenite tolerance in Arabidopsis and decreases nitrogen requirement under arsenite toxicity
physiological function
-
enzyme overexpression promotes the expression of Notch receptors and activates Akt signaling in glioma cells
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
(gamma-L-glutamyl)-L-serine
5-oxoproline + L-serine
show the reaction diagram
-
-
-
-
?
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
DL-proline + (S)-butan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
DL-proline + cyclohexylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
DL-proline + cyclopropylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-(tert-butylamino)-5-oxopentanoic acid
DL-proline + tert-butylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
DL-proline + propan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
DL-proline + (RS)-2-methylbutan-1-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
DL-proline + (S)-butan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
DL-proline + (2S)-2-methylbutan-1-amine
show the reaction diagram
-
-
-
-
?
2-N-(5-L-glutamyl)L-lysine
5-oxoproline + L-lysine
show the reaction diagram
-
-
-
?
5-(4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
substrate analog, 90% as effective as 5-glutamyl-L-2-aminobutyrate
-
?
5-glutamyl-glutathione
5-oxoproline + glutathione
show the reaction diagram
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
5-L-glutamyl-(+)-3-aminobutyric acid
5-oxoproline + (+)-3-aminbutyric acid
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-(+)-3-aminoisobutyric acid
5-oxoproline + (+)-3-aminoisobutyric acid
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-1-naphthylamide
5-oxoproline + naphthylamine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-glycine
5-oxoproline + 5-L-glutamyl-glycine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-alanine
5-oxoproline + 5-L-glutamyl-L-alanine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-amino acid
5-oxoproline + 5-L-glutamyl-L-amino acid
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-glutamate
5-oxoproline + 5-L-glutamyl-L-glutamate
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-glutamine
5-oxoproline + 5-L-glutamyl-L-glutamine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-leucine
5-oxoproline + 5-L-glutamyl-L-leucine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-lysine
5-oxoproline + 5-L-glutamyl-L-lysine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-phenylalanine
5-oxoproline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-proline
5-oxoproline + 5-L-glutamyl-L-proline
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-threonine
5-oxoproline + 5-L-glutamyl-L-threonine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-tyrosine
5-oxoproline + 5-L-glutamyl-L-tyrosine
show the reaction diagram
5-L-glutamyl-5-L-glutamyl-L-valine
5-oxoproline + 5-L-glutamyl-L-valine
show the reaction diagram
5-L-glutamyl-anilide
5-oxoproline + aniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-benzylamine
5-oxoproline + benzylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-beta-alanine
5-oxoproline + beta-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-beta-phenethylamine
5-oxoproline + beta-phenethylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-cyclopentylamine
5-oxoproline + cyclopentylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-cysteine
5-oxoproline + L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-ethylamine
5-oxoproline + ethylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
5-L-glutamyl-L-6-N-benzyloxycarbonyl-L-lysine
5-oxoproline + L-6-N-benzyloxycarbonyl-L-lysine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
5-L-glutamyl-L-alanine
5-oxoproline + L-alanine
show the reaction diagram
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
5-L-glutamyl-L-aspartate
5-oxoproline + L-aspartate
show the reaction diagram
5-L-glutamyl-L-cysteine
5-oxoproline + L-cysteine
show the reaction diagram
5-L-glutamyl-L-glutamate
5-oxoproline + L-glutamate
show the reaction diagram
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
5-L-glutamyl-L-isoleucine
5-oxoproline + L-isoleucine
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-leucine
5-oxoproline + L-leucine
show the reaction diagram
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
5-L-glutamyl-L-proline
5-oxoproline + L-proline
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-tyrosine
5-oxoproline + L-tyrosine
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-valine
5-oxoproline + L-valine
show the reaction diagram
5-L-glutamyl-methylamine
5-oxoproline + methylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-n-butylamine
5-oxoproline + butylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-n-propylamine
5-oxoproline + propylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-neohexylamine
5-oxoproline + neohexylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-neopentylamine
5-oxoproline + neopentylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-S-methyl-L-cysteine
5-oxoproline + S-methyl-L-cysteine
show the reaction diagram
D-gamma-glutamyl-O-[[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]carbonyl]-L-serine
5-oxoproline+O-[[(4-methyl-2-oxo-2H-1-benzopyran-7-yl)oxy]carbonyl]-L-serine
show the reaction diagram
-
-
-
-
?
gamma-glutamyl-Cys + H2O
5-oxoproline + L-cysteine
show the reaction diagram
-
assay at 30C, pH 8.0, 60 min, 48 microl substrate, reaction stopped with 6 microl HCl
-
-
?
gamma-glutamyl-L-alanine
5-oxoproline + alanine
show the reaction diagram
-
-
-
?
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
Gln-2-naphthylamide
pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln1-containing peptide
pyroglutamyl peptide
show the reaction diagram
-
-
-
-
?
Glu-2-naphthylamide
?
show the reaction diagram
-
-
-
-
?
glutathione
5-oxoproline + L-Cys-Gly
show the reaction diagram
L-2-amino-3-(3-n-butylureido)-propionic acid
?
show the reaction diagram
-
-
-
-
?
L-gamma-glutamyl-L-epsilon-lysine
5-oxoproline + L-lysine
show the reaction diagram
-
-
-
?
Nepsilon-(5-L-glutamyl)-D-lysine
?
show the reaction diagram
-
-
-
-
?
Nepsilon-(5-L-glutamyl)-L-lysine
5-oxoproline + L-lysine
show the reaction diagram
O-(n-butylcarbamyl)-L-serine
?
show the reaction diagram
-
-
-
-
?
S-(cyclohexylamine)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
S-(n-butylcarbamyl)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
S-(n-propylcarbamyl)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-aminoglutaryl-L-1-aminobutyrate
4-hydroxymercuribenzoate
-
0.5 mM, 43% inhibition of isoenzyme with pI 5.1
5-D-glutamyl-L-2-aminobutyrate
-
8 mM, 69% inhibition
benzimidazole
-
-
beta-aminoglutaryl-L-alanine
-
-
cystamine
-
10 mM, 57% inhibition of isoenzyme with pI 5.1
D-beta-aminoglutaryl-L-alanine
-
-
gamma-glutamyl cysteine disulfide
-
10 mM, 64% inhibition of isoenzyme with pI 5.1
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioredoxin
-
the enzyme is activated by 0.008 mM thioredoxin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.061
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.045
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.012
2-amino-5-(tert-butylamino)-5-oxopentanoic acid
-
kcat above 0.012 s-1, pH and temperature not specified in the publication
0.068
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
-
pH and temperature not specified in the publication
0.19
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.11
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.21
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
5
5-L-(threo-2-methyl)glutamyl-L-alpha-aminobutyrate
-
-
0.14
5-L-glutamyl-(+)-3-aminobutyric acid
-
pH and temperature not specified in the publication
0.33
5-L-glutamyl-(+)-3-aminoisobutyric acid
-
pH and temperature not specified in the publication
0.4 - 0.6
5-L-glutamyl-5-L-glutamyl-p-nitroanilide
0.1
5-L-glutamyl-anilide
-
pH and temperature not specified in the publication
0.56
5-L-glutamyl-benzylamine
-
pH and temperature not specified in the publication
0.91
5-L-glutamyl-beta-alanine
-
pH and temperature not specified in the publication
0.44
5-L-glutamyl-beta-phenethylamine
-
pH and temperature not specified in the publication
0.084
5-L-glutamyl-cyclopentylamine
-
pH and temperature not specified in the publication
0.36
5-L-glutamyl-ethylamine
-
pH and temperature not specified in the publication
1.9
5-L-Glutamyl-glycine
-
pH and temperature not specified in the publication
2 - 4.48
5-L-Glutamyl-L-alanine
6 - 12
5-L-Glutamyl-L-alpha-aminobutyrate
10 - 18
5-L-Glutamyl-L-glutamine
2.94
5-L-Glutamyl-L-phenylalanine
-
-
0.25
5-L-glutamyl-n-butylamine
-
pH and temperature not specified in the publication
0.32
5-L-glutamyl-n-propylamine
-
pH and temperature not specified in the publication
0.12
5-L-glutamyl-neohexylamine
-
pH and temperature not specified in the publication
0.61
5-L-glutamyl-neopentylamine
-
pH and temperature not specified in the publication
100
alpha-N-(gamma-L-glutamyl)-L-lysine
-
isoenzyme A
2 - 8
gamma-glutamyl-L-alanine
1.7 - 4.96
glutathione
0.27
Nepsilon-(5-L-glutamyl)-D-lysine
-
pH and temperature not specified in the publication
0.26
Nepsilon-(5-L-glutamyl)-L-lysine
-
pH and temperature not specified in the publication
20
O-(n-butylcarbamyl)-L-serine
-
pH and temperature not specified in the publication
0.19
S-(cyclohexylamine)-L-cysteine
-
pH and temperature not specified in the publication
0.091
S-(n-butylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
0.093
S-(n-propylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054
5-L-glutamyl-(+)-3-aminobutyric acid
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.071
5-L-glutamyl-(+)-3-aminoisobutyric acid
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.036
5-L-glutamyl-anilide
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.4
5-L-glutamyl-benzylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.18
5-L-glutamyl-beta-alanine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.17
5-L-glutamyl-beta-phenethylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.076
5-L-glutamyl-cyclopentylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.038
5-L-glutamyl-ethylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.14
5-L-Glutamyl-glycine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.02
5-L-glutamyl-methylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.21
5-L-glutamyl-n-butylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.23
5-L-glutamyl-n-propylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.18
5-L-glutamyl-neohexylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.035
5-L-glutamyl-neopentylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.08 - 18.3
gamma-glutamyl-L-alanine
0.113 - 52.8
glutathione
0.001
L-2-amino-3-(3-n-butylureido)-propionic acid
Oryctolagus cuniculus
-
kcat below 0.001 s-1, pH and temperature not specified in the publication
0.25
Nepsilon-(5-L-glutamyl)-D-lysine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.2
Nepsilon-(5-L-glutamyl)-L-lysine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
12
O-(n-butylcarbamyl)-L-serine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.26
S-(cyclohexylamine)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.27
S-(n-butylcarbamyl)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
0.24
S-(n-propylcarbamyl)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.058 - 1.24
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
88181
0.19 - 0.32
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
88186
0.21 - 0.22
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
88185
0.097 - 0.69
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
88179
0.38 - 0.51
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
88184
0.066 - 1.62
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
88180
0.38 - 0.55
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
88183
0.21
5-L-glutamyl-(+)-3-aminoisobutyric acid
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42181
0.19
5-L-glutamyl-anilide
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42185
0.71
5-L-glutamyl-benzylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42186
0.19
5-L-glutamyl-beta-alanine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42179
0.38
5-L-glutamyl-beta-phenethylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42187
0.9
5-L-glutamyl-cyclopentylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42184
0.11
5-L-glutamyl-ethylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42176
0.071
5-L-Glutamyl-glycine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
12393
0.83
5-L-glutamyl-n-butylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42178
0.72
5-L-glutamyl-n-propylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42177
1.45
5-L-glutamyl-neohexylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42183
0.057
5-L-glutamyl-neopentylamine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42182
0.023 - 25.2
glutathione
44
0.92
Nepsilon-(5-L-glutamyl)-D-lysine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42175
0.78
Nepsilon-(5-L-glutamyl)-L-lysine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
32938
0.6
O-(n-butylcarbamyl)-L-serine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42188
1.5
S-(cyclohexylamine)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42191
2.8
S-(n-butylcarbamyl)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42190
2.6
S-(n-propylcarbamyl)-L-cysteine
Oryctolagus cuniculus
-
pH and temperature not specified in the publication
42189
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.46 - 0.6
2-aminoglutaryl-L-alpha-aminobutyrate
0.3
D-beta-aminoglutaryl-L-alanine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0503
-
recombinant enzyme
0.06
-
activity in normal lung
0.24
-
activity in bronchoalveo carcinoma
0.36
-
activity in squamous cell carcinoma
0.77
-
activity in adenocarcinoma
2.7
L-gamma-glutamyl-L-epsilon-lysine
8.9
-
isoenzyme B, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide
9.7
-
isoenzyme B, substrate 5-L-glutamyl-L-alpha-aminobutyrate
38
-
isoenzyme A, substrate 5-L-glutamyl-L-alpha-aminobutyrate
40
-
isoenzyme A, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide
158
-
pI 4.6 isoenzyme
181
-
pI 5.1 isoenzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
cyclization of Glu
7.1 - 7.3
-
-
7.8 - 8.2
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4 - 7.5
-
pH 5.4: about 85% of maximal activity, pH 7.5: about 50% of maximal activity, cyclization of Glu
6 - 9.5
-
approx. half-maximal activity at pH 6 and 9.5
6.5 - 11.5
-
approx. half-maximal activity at pH 6.5 and 11.5
6.5 - 8.1
-
approx. half-maximal activity at pH 6.5 and 8.1
7 - 8
-
pH 7.0: about 60% of maximal activity, pH 8.0: optimum, cyclization of Gln
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
moderate expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
high concentration of GGCT transcripts
Manually annotated by BRENDA team
-
weak expression in granular and molecular layers
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
mammary gland epithelium
Manually annotated by BRENDA team
-
ER-1-24h, ER-1-1M cell produced
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
striated muscle, weak expression
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
high concentration of GGCT transcripts
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17300
recombinant, expressed in Escherichia coli
17330
theoretical, human A2LD1
18000
determined by gel filtration
25250
-
gel filtration
27500
-
gel filtration
30000
-
gel filtration
additional information
-
amino acid analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure is determined at 1.7 A resolution
-
the structures of GGACT, GGACT in complex with 5-oxoproline, and the E82Q mutant are solved to a resolution of 1.20, 1.20 and 0.98 A, respectively
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
-
purified enzyme, complete loss of activity after 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable
0C, at least 1 month
0C, pI 4.6-isoform, at least 2 months
-
0C, pI 5.1-isoform, 3 weeks, 30% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxyapatite
-
ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxylapatite
-
by affinity chromatography on glutathione-Sepharose 4B beads and subsequently by elution through digestion with PreScission protease
-
by Ni-agarose chromatography, subsequent removal of all additional N-terminal residues by cleavage with the catalytic domain of mouse ubiquitin specific protease 2
immunologically identical isoenzymes A and B, ammonium sulfate, gel filtration, carboxymethylcellulose, DEAE-cellulose
-
isoenzyme with pI 5.1: ammonium sulfate, heat, Sephadex G-75, DEAE-cellulose, thiol-Sepharose 4B, isoenzyme with pI 4.6: ammonium sulfate, heat, CM-cellulose, Sephadex G-75, DEAE-cellulose, ioselectric focusing, gel filtration
-
Ni-NTA affinity column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
pH 4.2, ammonium sulfate, heat, Sephadex G-75, carboxymethyl cellulose, DEAE-cellulose
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an ERpP cDNA library is constructed from mRNAs derived from ERpP cells
-
expressed in Escherichia coli BL21 pLys2(DE3) cells
-
expressed in Escherichia coli Rosetta gami B (DE3) cells
-
expressed in Saccharomyces cerevisiae strain HD9
-
into the vector pGEM-T for sequencing and subsequently into pHUE for expression of the protein in Escherichia coli BL21DE3 cells
into the vector pGEX6P1 for expression in Escherichia coli BL21 cells, into the plasmids pCIneo-FLAG, pCIneo-T7 and pEGFP-C1, into the vector pGEM-T
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is up-regulated in human glioma tissues and cell lines
-
the enzyme expression is increased in cancer tissue compared to normal tissue
-
the enzyme expression is upregulated in cancer cells (58% in cervical, 38% in lung, 72% in colon, and 46% in breast cancer)
-
the enzyme is upregulated after 0.025 mM arsenite treatment
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E82A
mutant, inactive
E82Q
mutant, inactive
E98A
-
inactive mutant enzyme
E98Q
-
inactive mutant enzyme
G23A
-
kcat/KM for gamma-glutamyl-L-alanine is 8.4fold lower than wild-type value
Y105F
-
kcat/KM for gamma-glutamyl-L-alanine is 650fold lower than wild-type value
Y125F
-
kcat/KM for gamma-glutamyl-L-alanine is 3.7fold lower than wild-type value
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine