Information on EC 2.3.2.4 - gamma-glutamylcyclotransferase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.3.2.4
-
RECOMMENDED NAME
GeneOntology No.
gamma-glutamylcyclotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(gamma-L-glutamyl)-L-amino acid = 5-oxoproline + L-amino acid
show the reaction diagram
mechanism
-
(gamma-L-glutamyl)-L-amino acid = 5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
aminoacyl group transfer
-
-
aminoacyl group transfer
-
-
PATHWAY
KEGG Link
MetaCyc Link
gamma-glutamyl cycle
-
Glutathione metabolism
-
SYSTEMATIC NAME
IUBMB Comments
(gamma-L-glutamyl)-L-amino-acid gamma-glutamyltransferase (cyclizing)
The enzyme acts on derivatives of L-glutamate, L-2-aminobutanoate, L-alanine and glycine.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
A2LD1
Q75223
-
C7orf24
-
-
C7orf24
O75223
-
cyclotransferase, gamma-glutamyl
-
-
-
-
gamma-glutamyl cyclotransferase
-
-
gamma-glutamyl cyclotransferase
-
-
gamma-glutamyl cyclotransferase
-
-
gamma-glutamyl-amino acid cyclotransferase
-
-
-
-
gamma-glutamylamine cyclotransferase
-
-
gamma-glutamyltranspeptidase
-
-
gamma-L-glutamylcyclotransferase
-
-
-
-
GGACT
Q75223
-
GGCT
-
-
GGCT
O75223
-
L-glutamic cyclase
-
-
-
-
protein C7orf24
-
-
protein C7orf24
-
-
{gamma}-glutamylamine cyclotransferase
Q75223
-
CAS REGISTRY NUMBER
COMMENTARY
9045-44-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ggt1-1/ggt4-1 mutants
-
-
Manually annotated by BRENDA team
guinea pig
-
-
Manually annotated by BRENDA team
patients with gastrointestinal fistula during and after enteral feeding
-
-
Manually annotated by BRENDA team
recombinant
-
-
Manually annotated by BRENDA team
house fly
-
-
Manually annotated by BRENDA team
tobacco, cv. Samsun
-
-
Manually annotated by BRENDA team
adult male Wistar rat
-
-
Manually annotated by BRENDA team
female Wistar
-
-
Manually annotated by BRENDA team
Holtzman
-
-
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Holtzman
Holtzman
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
-
?
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
-
?
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
Q75223
-
-
-
?
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
DL-proline + (S)-butan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
DL-proline + cyclohexylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
DL-proline + cycloprpylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-(tert-butylamino)-5-oxopentanoic acid
DL-proline + tert-butylamine
show the reaction diagram
-
-
-
-
?
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
DL-proline + propan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
DL-proline + (RS)-2-methylbutan-1-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
DL-proline + (S)-butan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
DL-proline + (2S)-2-methylbutan-1-amine
show the reaction diagram
-
-
-
-
?
2-N-(5-L-glutamyl)L-lysine
5-oxoproline + L-lysine
show the reaction diagram
-
-
-
?
5-(4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
substrate analog, 90% as effective as 5-glutamyl-L-2-aminobutyrate
-
?
5-glutamyl-glutathione
5-oxoproline + glutathione
show the reaction diagram
-
-
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
model substrate, in vivo and in vitro
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
model substrate, in vivo and in vitro
-
?
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate
3-methyl-5-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
model substrate, in vivo and in vitro
-
?
5-L-glutamyl-(+)-3-aminobutyric acid
5-oxoproline + (+)-3-aminbutyric acid
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-(+)-3-aminoisobutyric acid
5-oxoproline + (+)-3-aminoisobutyric acid
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-1-naphthylamide
5-oxoproline + naphthylamine
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-5-L-glutamyl-glycine
5-oxoproline + 5-L-glutamyl-glycine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-glycine
5-oxoproline + 5-L-glutamyl-glycine
show the reaction diagram
-
high activity
-
?
5-L-glutamyl-5-L-glutamyl-glycine
5-oxoproline + 5-L-glutamyl-glycine
show the reaction diagram
Rattus norvegicus Holtzman
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-alanine
5-oxoproline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-alanine
5-oxoproline + 5-L-glutamyl-L-alanine
show the reaction diagram
-
high activity
-
?
5-L-glutamyl-5-L-glutamyl-L-alanine
5-oxoproline + 5-L-glutamyl-L-alanine
show the reaction diagram
Rattus norvegicus Holtzman
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-amino acid
5-oxoproline + 5-L-glutamyl-L-amino acid
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-amino acid
5-oxoproline + 5-L-glutamyl-L-amino acid
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-amino acid
5-oxoproline + 5-L-glutamyl-L-amino acid
show the reaction diagram
Rattus norvegicus Holtzman
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-glutamate
5-oxoproline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-glutamate
5-oxoproline + 5-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-glutamine
5-oxoproline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-glutamine
5-oxoproline + 5-L-glutamyl-L-glutamine
show the reaction diagram
-
high activity
-
?
5-L-glutamyl-5-L-glutamyl-L-glutamine
5-oxoproline + 5-L-glutamyl-L-glutamine
show the reaction diagram
Rattus norvegicus Holtzman
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-leucine
5-oxoproline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-leucine
5-oxoproline + 5-L-glutamyl-L-leucine
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-lysine
5-oxoproline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-lysine
5-oxoproline + 5-L-glutamyl-L-lysine
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-phenylalanine
5-oxoproline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-phenylalanine
5-oxoproline + 5-L-glutamyl-L-phenylalanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-proline
5-oxoproline + 5-L-glutamyl-L-proline
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-proline
5-oxoproline + 5-L-glutamyl-L-proline
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-threonine
5-oxoproline + 5-L-glutamyl-L-threonine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-threonine
5-oxoproline + 5-L-glutamyl-L-threonine
show the reaction diagram
-
high activity
-
?
5-L-glutamyl-5-L-glutamyl-L-tyrosine
5-oxoproline + 5-L-glutamyl-L-tyrosine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-tyrosine
5-oxoproline + 5-L-glutamyl-L-tyrosine
show the reaction diagram
-
-
-
?
5-L-glutamyl-5-L-glutamyl-L-valine
5-oxoproline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
-
5-L-glutamyl-5-L-glutamyl-L-valine
5-oxoproline + 5-L-glutamyl-L-valine
show the reaction diagram
-
-
-
?
5-L-glutamyl-amine
5-oxoproline + amine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-anilide
5-oxoproline + aniline
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-benzylamine
5-oxoproline + benzylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-beta-alanine
5-oxoproline + beta-alanine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-beta-phenethylamine
5-oxoproline + beta-phenethylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-cyclopentylamine
5-oxoproline + cyclopentylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-cysteine
5-oxoproline + L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-ethylamine
5-oxoproline + ethylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
-
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-glycine
5-oxoproline + glycine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-glycine
5-oxoproline glycine
show the reaction diagram
-
poor substrate
-
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
-
25% as effective as 5-L-glutamyl-L-phenylalanine, no activity with D-glutamyl-isomer
-
?
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide
5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
5-L-glutamyl-L-6-N-benzyloxycarbonyl-L-lysine
5-oxoproline + L-6-N-benzyloxycarbonyl-L-lysine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alanine
5-L-oxoproline + L-alanine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-alpha-aminobutyrate
5-L-oxoproline + L-alpha-aminobutyrate
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
stereospecific
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with LD-, DD- and DL-isomers of 5-L-glutamyl-5-L-glutamyl-4-nitroanilide
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamine
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamine
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamine
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamine, no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine
-
-
-
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with: glutathione, i.e. gamma-L-glutamyl-L-cysteinylglycine
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamyl-beta-alanine, L-glutamyl-3-aminoisobutyrate
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with L-glutamyl-L-glutamyl-L-phenylalanine and L-glutamyl-L-glutamyl-1-naphthylamide, no activity with: alpha-L-glutamyl-glycine, alpha-L-glutamyl-L-alanine, alpha-L-glutamyl-L-tyrosine, alpha-L-glutamyl-2-naphthylamide, alpha-L-glutamyl-4-methoxy-2-beta-naphthylamide
-
-
-
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with D-glutamyl-2-aminobutyrate, L-(3-methyl)glutamyl-2-aminobutyrate, 3-aminoglutaryl-2-aminobutyrate, L-5-(N-methyl)glutamyl-2-aminobutyrate, 5-(5-methyl)glutamyl-2-aminobutyrate
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
highly specific for 5-L-glutamyl peptides
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
preferred substrates: 5-L-glutamyl-5-L-glutamyl-amino acids
-
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
preferred substrates: 5-L-glutamyl-5-L-glutamyl-amino acids
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
no activity with: 5-glutamyl-4-nitroanilide
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
Rattus norvegicus Holtzman
-
preferred substrates: 5-L-glutamyl-5-L-glutamyl-amino acids
i.e. 2-pyrrolidone-5-carboxylic acid
?
5-L-glutamyl-L-aspartate
5-oxoproline + L-aspartate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-aspartate
5-oxoproline + L-aspartate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-cysteine
5-oxoproline + L-cysteine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-L-cysteine
5-oxoproline + L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamate
5-oxoproline + L-glutamate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamate
5-oxoproline + L-glutamate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamate
5-oxoproline + L-glutamate
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamate
5-oxoproline + L-glutamate
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-glutamine
5-oxoproline + L-glutamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
best substrate
-
?
5-L-glutamyl-L-isoleucine
5-oxoproline + L-isoleucine
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-leucine
5-oxoproline + L-leucine
show the reaction diagram
-
-
-
?
5-L-glutamyl-L-leucine
5-oxoproline + L-leucine
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-leucine
5-oxoproline + L-leucine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
-
best substrate
-
-
?
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
-
70% as effective as L-glutamyl-L-phenylalanine
-
-
?
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
-
highly specific for, no activity with D-glutamyl-L-methionine
-
?
5-L-glutamyl-L-methionine
5-oxoproline + L-methionine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
best substrate
-
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
poor substrate
-
?
5-L-glutamyl-L-phenylalanine
5-oxoproline + L-phenylalanine
show the reaction diagram
-
best substrate
-
?
5-L-glutamyl-L-proline
5-oxoproline + L-proline
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-tyrosine
5-oxoproline + L-tyrosine
show the reaction diagram
-
very low activity
-
?
5-L-glutamyl-L-valine
5-oxoproline + L-valine
show the reaction diagram
-
very low activity, poor substrate
-
?
5-L-glutamyl-L-valine
5-oxoproline + L-valine
show the reaction diagram
-
10% as effective as 5-L-glutamyl-L-phenylalanine
-
?
5-L-glutamyl-methylamine
5-oxoproline + methylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-n-butylamine
5-oxoproline + butylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-n-propylamine
5-oxoproline + propylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-neohexylamine
5-oxoproline + neohexylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-neopentylamine
5-oxoproline + neopentylamine
show the reaction diagram
-
-
-
-
?
5-L-glutamyl-S-methyl-L-cysteine
5-oxoproline + S-methyl-L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-S-methyl-L-cysteine
5-oxoproline + S-methyl-L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-S-methyl-L-cysteine
5-oxoproline + S-methyl-L-cysteine
show the reaction diagram
-
-
-
?
5-L-glutamyl-S-methyl-L-cysteine
5-oxoproline + S-methyl-L-cysteine
show the reaction diagram
-
-
-
?
gamma-glutamyl-Cys + H2O
5-oxoproline + L-cysteine
show the reaction diagram
-
assay at 30C, pH 8.0, 60 min, 48 microl substrate, reaction stopped with 6 microl HCl
-
-
?
gamma-glutamyl-L-alanine
5-oxoproline + alanine
show the reaction diagram
O75223
-
-
-
?
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
involved in sclerotization process of white pupae
-
?
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
enzyme of L-glutamyl-cycle
-
?
Gln-2-naphthylamide
pyroglutamyl-2-naphthylamide + NH3
show the reaction diagram
-
-
-
-
?
Gln1-containing peptide
pyroglutamyl peptide
show the reaction diagram
-
-
-
-
?
Glu-2-naphthylamide
?
show the reaction diagram
-
-
-
-
?
L-2-amino-3-(3-n-butylureido)-propionic acid
?
show the reaction diagram
-
-
-
-
?
Nepsilon-(5-L-glutamyl)-D-lysine
?
show the reaction diagram
-
-
-
-
?
Nepsilon-(5-L-glutamyl)-L-lysine
5-oxoproline + L-lysine
show the reaction diagram
-
-
-
?
Nepsilon-(5-L-glutamyl)-L-lysine
5-oxoproline + L-lysine
show the reaction diagram
-
-
-
-
?
O-(n-butylcarbamyl)-L-serine
?
show the reaction diagram
-
-
-
-
?
S-(cyclohexylamine)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
S-(n-butylcarbamyl)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
S-(n-propylcarbamyl)-L-cysteine
?
show the reaction diagram
-
-
-
-
?
L-gamma-glutamyl-L-epsilon-lysine
5-oxoproline + L-lysine
show the reaction diagram
Q75223
-
-
-
?
additional information
?
-
-
N-terminal pyroglutamate formation is a posttranslational event in the processing of bioactive neuropeptides such as thyrotropin-releasing hormone and neurotensin during their maturation in the secretory pathway. The enzyme also catalyzes N-terminal glutamate cyclization
-
-
-
additional information
?
-
-
the enzyme does not act on L-glutamine, alpha-(5-L-glutamyl)-L-lysine, 5-L-glutamyl-L-methionine, or 5-L-glutamyl-L-glutamine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
-
?
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
-
-
-
?
(gamma-L-glutamyl)-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
Q75223
-
-
-
?
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
involved in sclerotization process of white pupae
-
?
gamma-L-glutamyl-L-amino acid
5-oxoproline + L-amino acid
show the reaction diagram
-
enzyme of L-glutamyl-cycle
-
?
additional information
?
-
-
N-terminal pyroglutamate formation is a posttranslational event in the processing of bioactive neuropeptides such as thyrotropin-releasing hormone and neurotensin during their maturation in the secretory pathway. The enzyme also catalyzes N-terminal glutamate cyclization
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-aminoglutaryl-L-1-aminobutyrate
-
-
2-aminoglutaryl-L-1-aminobutyrate
-
-
2-aminoglutaryl-L-1-aminobutyrate
-
-
2-aminoglutaryl-L-1-aminobutyrate
-
8 mM, 96% inhibition
4-hydroxymercuribenzoate
-
0.5 mM, 43% inhibition of isoenzyme with pI 5.1
5-D-glutamyl-L-2-aminobutyrate
-
8 mM, 69% inhibition
benzimidazole
-
-
beta-aminoglutaryl-L-alanine
-
-
cystamine
-
10 mM, 57% inhibition of isoenzyme with pI 5.1
D-beta-aminoglutaryl-L-alanine
-
-
gamma-glutamyl cysteine disulfide
-
10 mM, 64% inhibition of isoenzyme with pI 5.1
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.07
-
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.061
-
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.045
-
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.012
-
2-amino-5-(tert-butylamino)-5-oxopentanoic acid
-
kcat above 0.012 s-1, pH and temperature not specified in the publication
0.068
-
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
-
pH and temperature not specified in the publication
0.19
-
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.11
-
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
0.21
-
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
5
-
5-L-(threo-2-methyl)glutamyl-L-alpha-aminobutyrate
-
-
0.14
-
5-L-glutamyl-(+)-3-aminobutyric acid
-
pH and temperature not specified in the publication
-
0.33
-
5-L-glutamyl-(+)-3-aminoisobutyric acid
-
pH and temperature not specified in the publication
-
0.4
-
5-L-glutamyl-5-L-glutamyl-p-nitroanilide
-
-
0.6
-
5-L-glutamyl-5-L-glutamyl-p-nitroanilide
-
-
0.1
-
5-L-glutamyl-anilide
-
pH and temperature not specified in the publication
-
0.56
-
5-L-glutamyl-benzylamine
-
pH and temperature not specified in the publication
-
0.91
-
5-L-glutamyl-beta-alanine
-
pH and temperature not specified in the publication
-
0.44
-
5-L-glutamyl-beta-phenethylamine
-
pH and temperature not specified in the publication
-
0.084
-
5-L-glutamyl-cyclopentylamine
-
pH and temperature not specified in the publication
-
0.36
-
5-L-glutamyl-ethylamine
-
pH and temperature not specified in the publication
-
1.9
-
5-L-Glutamyl-glycine
-
pH and temperature not specified in the publication
2
-
5-L-Glutamyl-L-alanine
-
-
2.2
-
5-L-Glutamyl-L-alanine
-
-
4.48
-
5-L-Glutamyl-L-alanine
-
-
6
-
5-L-Glutamyl-L-alpha-aminobutyrate
-
isoenzyme with pI 5.1, in extract, Km increases 4-10fold during purification
6.6
-
5-L-Glutamyl-L-alpha-aminobutyrate
-
isoenzyme with pI 4.6
8
-
5-L-Glutamyl-L-alpha-aminobutyrate
-
isoenzyme B
12
-
5-L-Glutamyl-L-alpha-aminobutyrate
-
isoenzyme A
10
-
5-L-Glutamyl-L-glutamine
-
isoenzyme B
18
-
5-L-Glutamyl-L-glutamine
-
isoenzyme A
2.94
-
5-L-Glutamyl-L-phenylalanine
-
-
0.25
-
5-L-glutamyl-n-butylamine
-
pH and temperature not specified in the publication
-
0.32
-
5-L-glutamyl-n-propylamine
-
pH and temperature not specified in the publication
-
0.12
-
5-L-glutamyl-neohexylamine
-
pH and temperature not specified in the publication
-
0.61
-
5-L-glutamyl-neopentylamine
-
pH and temperature not specified in the publication
-
100
-
alpha-N-(gamma-L-glutamyl)-L-lysine
-
isoenzyme A
2
-
gamma-glutamyl-L-alanine
-
recombinant wild type enzyme
5.6
-
gamma-glutamyl-L-alanine
-
mutant enzyme Y105F
5.8
-
gamma-glutamyl-L-alanine
-
mutant enzyme Y125F
0.27
-
Nepsilon-(5-L-glutamyl)-D-lysine
-
pH and temperature not specified in the publication
-
0.26
-
Nepsilon-(5-L-glutamyl)-L-lysine
-
pH and temperature not specified in the publication
20
-
O-(n-butylcarbamyl)-L-serine
-
pH and temperature not specified in the publication
-
0.19
-
S-(cyclohexylamine)-L-cysteine
-
pH and temperature not specified in the publication
-
0.091
-
S-(n-butylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
-
0.093
-
S-(n-propylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
-
8
-
gamma-glutamyl-L-alanine
-
mutant enzyme G23A
additional information
-
additional information
-
kinetics
-
additional information
-
additional information
-
Km-values for Gln-containing peptides
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.054
-
5-L-glutamyl-(+)-3-aminobutyric acid
-
pH and temperature not specified in the publication
-
0.071
-
5-L-glutamyl-(+)-3-aminoisobutyric acid
-
pH and temperature not specified in the publication
-
0.036
-
5-L-glutamyl-anilide
-
pH and temperature not specified in the publication
-
0.4
-
5-L-glutamyl-benzylamine
-
pH and temperature not specified in the publication
-
0.18
-
5-L-glutamyl-beta-alanine
-
pH and temperature not specified in the publication
-
0.17
-
5-L-glutamyl-beta-phenethylamine
-
pH and temperature not specified in the publication
-
0.076
-
5-L-glutamyl-cyclopentylamine
-
pH and temperature not specified in the publication
-
0.038
-
5-L-glutamyl-ethylamine
-
pH and temperature not specified in the publication
-
0.14
-
5-L-Glutamyl-glycine
-
pH and temperature not specified in the publication
0.02
-
5-L-glutamyl-methylamine
-
pH and temperature not specified in the publication
-
0.21
-
5-L-glutamyl-n-butylamine
-
pH and temperature not specified in the publication
-
0.23
-
5-L-glutamyl-n-propylamine
-
pH and temperature not specified in the publication
-
0.18
-
5-L-glutamyl-neohexylamine
-
pH and temperature not specified in the publication
-
0.035
-
5-L-glutamyl-neopentylamine
-
pH and temperature not specified in the publication
-
0.08
-
gamma-glutamyl-L-alanine
-
mutant enzyme Y105F
6.6
-
gamma-glutamyl-L-alanine
-
mutant enzyme G23A
14.7
-
gamma-glutamyl-L-alanine
-
mutant enzyme Y125F
18.3
-
gamma-glutamyl-L-alanine
-
recombinant wild type enzyme
0.001
-
L-2-amino-3-(3-n-butylureido)-propionic acid
-
kcat below 0.001 s-1, pH and temperature not specified in the publication
-
0.25
-
Nepsilon-(5-L-glutamyl)-D-lysine
-
pH and temperature not specified in the publication
-
0.2
-
Nepsilon-(5-L-glutamyl)-L-lysine
-
pH and temperature not specified in the publication
12
-
O-(n-butylcarbamyl)-L-serine
-
pH and temperature not specified in the publication
-
0.26
-
S-(cyclohexylamine)-L-cysteine
-
pH and temperature not specified in the publication
-
0.27
-
S-(n-butylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
-
0.24
-
S-(n-propylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.058
-
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324730
1.24
-
2-amino-5-(butan-2-ylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324730
0.19
-
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324735
0.32
-
2-amino-5-(cyclohexylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324735
0.21
-
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324734
0.22
-
2-amino-5-(cyclopropylamino)-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324734
0.097
-
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
-
pH and temperature not specified in the publication
324728
0.69
-
2-amino-5-oxo-5-(propan-2-ylamino)pentanoic acid
-
pH and temperature not specified in the publication
324728
0.38
-
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324733
0.51
-
2-amino-5-[(2-methylbutyl)amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324733
0.066
-
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324729
1.62
-
2-amino-5-[(2S)-butan-2-ylamino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324729
0.38
-
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324732
0.55
-
2-amino-5-[[(2S)-2-methylbutyl]amino]-5-oxopentanoic acid
-
pH and temperature not specified in the publication
324732
0.21
-
5-L-glutamyl-(+)-3-aminoisobutyric acid
-
pH and temperature not specified in the publication
0
0.19
-
5-L-glutamyl-anilide
-
pH and temperature not specified in the publication
0
0.71
-
5-L-glutamyl-benzylamine
-
pH and temperature not specified in the publication
0
0.19
-
5-L-glutamyl-beta-alanine
-
pH and temperature not specified in the publication
0
0.38
-
5-L-glutamyl-beta-phenethylamine
-
pH and temperature not specified in the publication
0
0.9
-
5-L-glutamyl-cyclopentylamine
-
pH and temperature not specified in the publication
0
0.11
-
5-L-glutamyl-ethylamine
-
pH and temperature not specified in the publication
0
0.071
-
5-L-Glutamyl-glycine
-
pH and temperature not specified in the publication
5335
0.83
-
5-L-glutamyl-n-butylamine
-
pH and temperature not specified in the publication
0
0.72
-
5-L-glutamyl-n-propylamine
-
pH and temperature not specified in the publication
0
1.45
-
5-L-glutamyl-neohexylamine
-
pH and temperature not specified in the publication
0
0.057
-
5-L-glutamyl-neopentylamine
-
pH and temperature not specified in the publication
0
0.92
-
Nepsilon-(5-L-glutamyl)-D-lysine
-
pH and temperature not specified in the publication
0
0.78
-
Nepsilon-(5-L-glutamyl)-L-lysine
-
pH and temperature not specified in the publication
140483
0.6
-
O-(n-butylcarbamyl)-L-serine
-
pH and temperature not specified in the publication
0
1.5
-
S-(cyclohexylamine)-L-cysteine
-
pH and temperature not specified in the publication
0
2.8
-
S-(n-butylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
0
2.6
-
S-(n-propylcarbamyl)-L-cysteine
-
pH and temperature not specified in the publication
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.46
-
2-aminoglutaryl-L-alpha-aminobutyrate
-
-
0.6
-
2-aminoglutaryl-L-alpha-aminobutyrate
-
-
0.3
-
D-beta-aminoglutaryl-L-alanine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0503
-
-
recombinant enzyme
0.06
-
-
activity in normal lung
0.24
-
-
activity in bronchoalveo carcinoma
0.36
-
-
activity in squamous cell carcinoma
0.77
-
-
activity in adenocarcinoma
2.7
-
Q75223
L-gamma-glutamyl-L-epsilon-lysine
8.9
-
-
isoenzyme B, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide
9.7
-
-
isoenzyme B, substrate 5-L-glutamyl-L-alpha-aminobutyrate
33.8
-
-
-
38
-
-
isoenzyme A, substrate 5-L-glutamyl-L-alpha-aminobutyrate
40
-
-
isoenzyme A, substrate 5-L-glutamyl-5-L-glutamyl-p-nitroanilide
70.7
-
-
-
100
-
-
-
158
-
-
pI 4.6 isoenzyme
181
-
-
pI 5.1 isoenzyme
additional information
-
-
spectrometric assay
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
cyclization of Glu
7.1
7.3
-
-
7.8
8.2
-
borate buffer
8
-
-
cyclization of Gln
8
-
-
assay at
8
-
Q75223
activity assay
additional information
-
-
two isozymic forms of different pI-values: pI 4.6 and 5.1
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
7.5
-
pH 5.4: about 85% of maximal activity, pH 7.5: about 50% of maximal activity, cyclization of Glu
6
9.5
-
approx. half-maximal activity at pH 6 and 9.5
6.5
11.5
-
approx. half-maximal activity at pH 6.5 and 11.5
6.5
8.1
-
approx. half-maximal activity at pH 6.5 and 8.1
7
8
-
pH 7.0: about 60% of maximal activity, pH 8.0: optimum, cyclization of Gln
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
37
-
Q75223
activity assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
moderate expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
high concentration of GGCT transcripts
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
weak expression in granular and molecular layers
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
mammary gland epithelium
Manually annotated by BRENDA team
-
ER-1-24h, ER-1-1M cell produced
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
very high activity
Manually annotated by BRENDA team
Rattus norvegicus Holtzman, Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
striated muscle, weak expression
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
high concentration of GGCT transcripts
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
-
weak/moderate expression
Manually annotated by BRENDA team
-
high concentration of GGCT transcripts
Manually annotated by BRENDA team
-
weak expression
Manually annotated by BRENDA team
additional information
-
-
Manually annotated by BRENDA team
additional information
-
-
Manually annotated by BRENDA team
additional information
-
-
Manually annotated by BRENDA team
additional information
-
tissue distribution
Manually annotated by BRENDA team
additional information
-
-
Manually annotated by BRENDA team
additional information
-
not detected in bone marrow, eye, Fallopian tube, heart, ovary, pancreas, pituitary gland, skin, spinal cord, thymus, placenta, and prostate gland
Manually annotated by BRENDA team
additional information
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
17300
-
Q75223
recombinant, expressed in Escherichia coli
17330
-
Q75223
theoretical, human A2LD1
18000
-
Q75223
determined by gel filtration
25250
-
-
gel filtration
27500
-
-
gel filtration
30000
-
-
gel filtration
additional information
-
-
amino acid analysis
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 20994, calculated from sequence; x * 21000, recombinant enzyme, SDS-PAGE
monomer
-
1 * 27000, SDS-PAGE
monomer
Q75223
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
structure is determined at 1.7 A resolution
-
the structures of GGACT, GGACT in complex with 5-oxoproline, and the E82Q mutant are solved to a resolution of 1.20, 1.20 and 0.98 A, respectively
Q75223
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
57
-
-
purified enzyme, complete loss of activity after 5 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, stable
-
0C, at least 1 month
-
-20C, stable
-
0C, at least 1 month
-
0C, pI 4.6-isoform, at least 2 months
-
0C, pI 5.1-isoform, 3 weeks, 30% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxyapatite
-
by affinity chromatography on glutathione-Sepharose 4B beads and subsequently by elution through digestion with PreScission protease
-
by Ni-agarose chromatography, subsequent removal of all additional N-terminal residues by cleavage with the catalytic domain of mouse ubiquitin specific protease 2
Q75223
pH 4.2, ammonium sulfate, heat, Sephadex G-75, carboxymethyl cellulose, DEAE-cellulose
-
ammonium sulfate, Sephadex G-75, DEAE-cellulose, hydroxylapatite
-
immunologically identical isoenzymes A and B, ammonium sulfate, gel filtration, carboxymethylcellulose, DEAE-cellulose
-
isoenzyme with pI 5.1: ammonium sulfate, heat, Sephadex G-75, DEAE-cellulose, thiol-Sepharose 4B, isoenzyme with pI 4.6: ammonium sulfate, heat, CM-cellulose, Sephadex G-75, DEAE-cellulose, ioselectric focusing, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
into the vector pGEM-T for sequencing and subsequently into pHUE for expression of the protein in Escherichia coli BL21DE3 cells
Q75223
into the vector pGEX6P1 for expression in Escherichia coli BL21 cells, into the plasmids pCIneo-FLAG, pCIneo-T7 and pEGFP-C1, into the vector pGEM-T
-
an ERpP cDNA library is constructed from mRNAs derived from ERpP cells
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the enzyme expression is upregulated in cancer cells (58% in cervical, 38% in lung, 72% in colon, and 46% in breast cancer)
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E82A
Q75223
mutant, inactive
E82Q
Q75223
mutant, inactive
E98A
-
inactive mutant enzyme
E98Q
-
inactive mutant enzyme
G23A
-
kcat/KM for gamma-glutamyl-L-alanine is 8.4fold lower than wild-type value
Y105F
-
kcat/KM for gamma-glutamyl-L-alanine is 650fold lower than wild-type value
Y125F
-
kcat/KM for gamma-glutamyl-L-alanine is 3.7fold lower than wild-type value
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
in patients with gastrointestinal fistula, levels of alkaline phosphatase and gamma-glutamylcyclotransferase increase significantly on the third day after beginning of enteral feeding and decrease gradually afterwards. The longer the total parenteral nutrition lasted, the more severe the enteral refeeding syndrome becomes. Early enteral nutrition is useful in preventing it
medicine
-
enzyme overexpression defines a subgroup of breast tumors with poor clinical outcome. The enzyme is a cancer biomarker and a serological marker