Information on EC 2.3.2.3 - lysyltransferase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.3.2.3
-
RECOMMENDED NAME
GeneOntology No.
lysyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-lysyl-tRNALys + phosphatidylglycerol = tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
aminoacyl group transfer
-
-
aminoacyl group transfer
-
-
aminoacyl group transfer
Q2G2M2
-
SYSTEMATIC NAME
IUBMB Comments
L-lysyl-tRNALys:phosphatidylglycerol 3-O-lysyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
LPG synthetase
-
-
Lys-tRNA(Lys) phosphatidylglycerol transferase
-
-
lysophosphatidylglycerol synthetase
-
-
lysyl-transferase-lysyl-tRNA synthetase
-
-
lysyltransferase
Q2G2M2
-
MprF
-
a membrane protein with both lysylphosphatidylglycerol synthase and flippase activities
MprF protein
-
-
MprF protein
Q2G2M2
-
mprF-lysU protein
-
-
MprF2
-
the enzyme encompasses a lysyl-phosphatidylglycerol synthase and a lysyl-phosphatidylglycerol flippase domain
multiple peptide resistance factor protein
Q2G2M2
-
CAS REGISTRY NUMBER
COMMENTARY
37257-20-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
a MprF knockout mutant demonstrates a substantial increase in the phosphatidylglycerol:lysylphosphatidylglycerol ratio of the membrane
metabolism
-
the C-terminal domain of the enzyme is responsible for the synthesis of lysylphosphatidylglycerol
physiological function
-
MprF2-mediated lysyl-phosphatidylglycerol production confers cationic antimicrobial peptide susceptibility with 6fold decreased daptomycin susceptibility or 4fold decreased gallidermin and nisin susceptibility compared to the Staphylococcus aureus mprF deletion mutant
physiological function
-
the mprFgene confers daptomycin resistance
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
Q2G2M2
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
2'- (not 3'-) deoxy-analogue of phosphatidylglycerol can also act as L-lysyl-acceptor
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
Bacillus subtilis, Bacillus subtilis Marburg
-
enzyme is involved in synthesis of lipids
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
-
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
Q2G2M2
-
-
-
?
L-lysyl-tRNALys + phosphatidylglycerol
tRNALys + 3-phosphatidyl-1'-(3'-O-L-lysyl)glycerol
show the reaction diagram
Bacillus subtilis, Bacillus subtilis Marburg
-
enzyme is involved in synthesis of lipids
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
anionic surfactant, e.g. sodium-salt of a fatty acid, and high ionic strength lead to activation
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0069
-
L-Lysyl-tRNA
-
-
0.056
-
phosphatidylglycerol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00000072
-
-
supernatant 10000 x g
0.00000143
-
-
cell crude extract
0.00000326
-
-
membrane fraction
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2
-
-
steady state assay
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
steady state assay
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
septal membranes
Manually annotated by BRENDA team
-
an integral membrane protein
Manually annotated by BRENDA team
Bacillus subtilis Marburg
-
septal membranes
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
39000
-
Q2G2M2
theoretical, MprF(-14) mutant, truncated protein consisting of residues 510-840
48000
-
Q2G2M2
theoretical, MprF(-12) mutant, truncated protein consisting of residues 437-840
56000
-
Q2G2M2
theoretical, MprF(-10) mutant, truncated protein consisting of residues 363-840
67000
-
Q2G2M2
theoretical, MprF(-8) mutant, truncated protein consisting of residues 274-840; theoretical, MprF(-C) mutant, truncated protein consisting of residues 1-586
75000
-
Q2G2M2
theoretical, MprF(-6) mutant, truncated protein consisting of residues 219-840
80000
-
Q2G2M2
theoretical, MprF(-4) mutant, truncated protein consisting of residues 157-840
88000
-
Q2G2M2
theoretical, MprF(-2) mutant, truncated protein consisting of residues 84-840
97000
-
Q2G2M2
theoretical, MprF wild-type
100000
-
-
determined by SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, membrane-bound enzyme extracted with organic solvents, t1/2: 3-5 days
-
-20C, native membrane-bound enzyme, several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
membrane fractions are prepared by differential centrifugation
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
into the vector pET33b for expression in Escherichia coli Rosetta2 DE3, C41 and C43 cells
-
expressed in a Staphylococcus aureus mprF deletion mutant
-
for the construction of lysX deletion and complemented derivative Mycobacterium tuberculosis strains
-
expression in Escherichia coli
-
into the vector pET28
Q2G2M2
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D546A
Q2G2M2
MprF(-8) mutant, replacement results in slightly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein
D731A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
E624A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
E685A
Q2G2M2
MprF(-8) mutant, replacement results in strongly reduced production of lysyl-phosphatidylglycerol, same result is obtained when the mutation is introduced into the full-length MprF protein
K547A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
K621A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
K806A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
MprF(-10)
Q2G2M2
mutant, truncated protein consisting of residues 363-840
MprF(-12)
Q2G2M2
mutant, truncated protein consisting of residues 437-840
MprF(-14)
Q2G2M2
mutant, truncated protein consisting of residues 510-840
MprF(-2)
Q2G2M2
mutant, truncated protein consisting of residues 84-840
MprF(-4)
Q2G2M2
mutant, truncated protein consisting of residues 157-840
MprF(-6)
Q2G2M2
mutant, truncated protein consisting of residues 219-840
MprF(-8)
Q2G2M2
mutant, truncated protein consisting of residues 274-840
MprF(-C)
Q2G2M2
mutant, truncated protein consisting of residues 1-586
R734A
Q2G2M2
MprF(-8) mutant, exchange leads to complete abrogation of lysyl-phosphatidylglycerol production, same result is obtained when the mutation is introduced into the full-length MprF protein
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
a method to obtain a stable enriched membrane fraction containing MprF, and the techniques necessary to quantitatively monitor its activity in vitro and in vivo is reported
medicine
Q2G2M2
MprF could be a target for new anti-virulence drugs