Information on EC 2.3.2.11 - alanylphosphatidylglycerol synthase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.3.2.11
-
RECOMMENDED NAME
GeneOntology No.
alanylphosphatidylglycerol synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminoacyl group transfer
-
-
-
-
aminoacyl group transfer
-
-
SYSTEMATIC NAME
IUBMB Comments
L-alanyl-tRNAAla:phosphatidylglycerol alanyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ala-PG synthase
-
-
Ala-PG synthase
-
-
alanyl phosphatidylglycerol synthetase
-
-
-
-
alanyl-phosphatidylglycerol synthase
-
-
alanylphosphatidylglycerol synthase
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-
O-alanylphosphatidylglycerol synthase
-
-
-
-
synthase, O-alanylphosphatidylglycerol
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37257-27-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Clostridium welchii
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
MprF1-mediated alanyl-phosphatidylglycerol production in Staphylococcus aureus does not affect susceptibility to gallidermin and nisin and leads to only a minor decrease in daptomycin susceptibility. Coexpression of the enzyme with flippase domains of lysyl-phosphatidylglycerol synthesizing MprF proteins leads to a wild type level of daptomycin susceptibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
-
20% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
-
-
-
-
?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
-
100% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)
?
show the reaction diagram
-
20% activity compared to phosphatidylglycerol
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
-
-
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
-
100% activity
-
-
?
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
Clostridium welchii
-
other L-alanyl-tRNAs than L-alanyl-tRNAAla are no substrates
-
?
microhelix A + phosphatidylglycerol
?
show the reaction diagram
-
90% activity compared to L-alanyl-tRNA
-
-
?
microhelix B + phosphatidylglycerol
?
show the reaction diagram
-
40% activity compared to L-alanyl-tRNA
-
-
?
microhelix C + phosphatidylglycerol
?
show the reaction diagram
-
-
-
-
?
microhelix D + phosphatidylglycerol
?
show the reaction diagram
-
-
-
-
?
microhelix E + phosphatidylglycerol
?
show the reaction diagram
-
90% activity compared to L-alanyl-tRNA
-
-
?
additional information
?
-
Clostridium welchii
-
no substrates are N-acetylalanyl-tRNA, lactyl-tRNA, alanyl-tRNACys and phenylalanyl-tRNAAla
-
-
-
additional information
?
-
-
1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-alanyl-tRNAAla + phosphatidylglycerol
tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
enzyme catalysis is not dependent on metal ions
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
20% residual activity at 20 mM
additional information
-
in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.7
-
Clostridium welchii
-
-
7.8
-
-
in vivo activity assay
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Clostridium welchii
-
assay at
35
-
-
in vitro activity assay; in vivo activity assay
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Clostridium welchii
-
-
Manually annotated by BRENDA team
-
a transmembrane protein which is located in the inner membrane
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
34000
-
-
gel filtration
84000
-
-
determined by SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 38000, SDS-PAGE; 1 * 38720, calculated from amino acid sequence
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glycerol stabilizes during storage
Clostridium welchii
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20°C, 3 weeks, 10 mM Tris/HCl, pH 8.0, 10 mM 2-mercaptoethanol, 30% glycerol
Clostridium welchii
-
-60°C, several months
Clostridium welchii
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
from Escherichia coli membranes, a discontinuous sucrose gradient centrifugation is performed, further purified using chelating sepharose, which is loaded with nickel ions
-
glutathione Sepharose column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in a mprF deletion mutant of Staphylococcus aureus
-
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells
-
into the vectors pET22b+, pUCP20T, pBAD-His-A, pBADmyc-His-A
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene expression is found significantly up-regulated under acidic conditions
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D579A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
D579N
-
the mutant shows decreased activity (5%) compared to the wild type enzyme
D710A
-
the mutant has 26% of wild type activity
D710N
-
the mutant has 30% of wild type activity
D765A
-
inactive
D765N
-
inactive
E657D
-
the mutant shows decreased activity (4%) compared to the wild type enzyme
E657Q
-
the mutant has 21% of wild type activity
E658D
-
the mutant has 28% of wild type activity
E658Q
-
the mutant has 19% of wild type activity
E720Q
-
the mutant has 10% of wild type activity
K580Q
-
inactive
K580S
-
inactive
K654Q
-
the mutant has 65% of wild type activity
K654S
-
the mutant has 46% of wild type activity
K840Q
-
inactive
K840S
-
inactive
K842Q
-
the mutant has wild type activity
K842S
-
the mutant has 50% of wild type activity
M778A
-
the mutant has 50% of wild type activity
R768Q
-
inactive
R768S
-
inactive
R837Q
-
the mutant has wild type activity
R837S
-
the mutant has 65% of wild type activity
S709A
-
the mutant has 5% of wild type activity
S709N
-
the mutant has 17% of wild type activity
S724A
-
the mutant shows decreased activity (7%) compared to the wild type enzyme
S724N
-
inactive
S763A
-
the mutant has 23% of wild type activity
S763N
-
the mutant has 11% of wild type activity
Y635A
-
inactive
Y732A
-
the mutant shows decreased activity (3%) compared to the wild type enzyme
Y831A
-
inactive