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Information on EC 2.3.1.87 - aralkylamine N-acetyltransferase and Organism(s) Gallus gallus and UniProt Accession P79774

for references in articles please use BRENDA:EC2.3.1.87
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IUBMB Comments
Narrow specificity towards 2-arylethylamines, including serotonin (5-hydroxytryptamine), tryptamine, 5-methoxytryptamine and phenylethylamine. This is the penultimate enzyme in the production of melatonin (5-methoxy-N-acetyltryptamine) and controls its synthesis (cf. EC 2.1.1.4, acetylserotonin O-methyltransferase). Differs from EC 2.3.1.5 arylamine N-acetyltransferase.
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This record set is specific for:
Gallus gallus
UNIPROT: P79774
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The taxonomic range for the selected organisms is: Gallus gallus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
aanat, aa-nat, snat2, arylalkylamine n-acetyltransferase, snat1, aanat2, aanat1, serotonin-n-acetyltransferase, aralkylamine n-acetyltransferase, bm-iaanat, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
serotonin acetylase
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AA-NAT
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-
-
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AANAT
acetyl-CoA:aralkylamine N-acetyltransferase
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-
-
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acetyltransferase, arylalkylamine N-
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-
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arylalkylamine N-acetyltransferase
N-acetyltransferase
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-
-
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serotonin acetylase
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-
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serotonin acetyltransferase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:2-arylethylamine N-acetyltransferase
Narrow specificity towards 2-arylethylamines, including serotonin (5-hydroxytryptamine), tryptamine, 5-methoxytryptamine and phenylethylamine. This is the penultimate enzyme in the production of melatonin (5-methoxy-N-acetyltryptamine) and controls its synthesis (cf. EC 2.1.1.4, acetylserotonin O-methyltransferase). Differs from EC 2.3.1.5 arylamine N-acetyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
92941-56-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
key enzyme of circadian rhythm of melatonin synthesis
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-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
?
additional information
?
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enzyme activity in cultured cells is suppressed by light, as it is in vivo. The ability to express circadian regulation of the enzyme activity is an intrinsic property of retinal cells that can develop in vitro
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
key enzyme of circadian rhythm of melatonin synthesis
-
-
?
additional information
?
-
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enzyme activity in cultured cells is suppressed by light, as it is in vivo. The ability to express circadian regulation of the enzyme activity is an intrinsic property of retinal cells that can develop in vitro
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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exposing chickens to light in the middle of the night increased the apparent Km of AANAT for tryptamine by about 10fold. The Km began to increase within 5 min of exposure to light and reached a maximum at about 30 min
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
exposure of dark-adapted animals to near ultraviolet light significantly decreases melatonin content and the activity of serotonin N-acetyltransferase
Manually annotated by BRENDA team
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cultured photoreceptor cells of embryonic retina
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SNAT_CHICK
205
0
23187
Swiss-Prot
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000 - 100000
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gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
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AANAT in dark-adapted retina is bound to 14-3-3 protein. This interaction is regulated by light and phosphorylation. Phosphorylation of AANAT in darkness promotes the association with 14-3-3, which decreases the Km of the enzyme for substrate and protects the enzyme from dephosphorylation and degradation. Light exposure results in dephosphorylation of AANAT and dissociation from the complex with 14-3-3 proteins, thereby increasing the Km of the enzyme. Dissociation of the complex leads to subsequent proteasomal proteolysis of AANAT
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohtomi, M.; Sasaki, M.; Deguchi, T.
Two arylamine N-acetyltransferases from chicken pineal gland as identified by cDNA cloning
Eur. J. Biochem.
185
253-261
1989
Gallus gallus
Manually annotated by BRENDA team
Rodriguez-Cabello, J.C.; Agapito, M.T.; Garcia-Herrero, I.; Recio, J.M.
Effects of EGTA and calmodulin, neutral thiol proteinases and protein kinase C inhibitors on loss of chicken pineal serotonin N-acetyltransferase activity
J. Comp. Physiol. B
159
583-588
1989
Gallus gallus
Manually annotated by BRENDA team
Ivanova, T.N.; Michael Iuvone, P.
Melatonin synthesis in retina: circadian regulation of arylalkylamine N-acetyltransferase activity in cultured photoreceptor cells of embryonic chicken retina
Brain Res.
973
56-63
2003
Gallus gallus
Manually annotated by BRENDA team
Tosini, G.; Chaurasia, S.S.; Michael Iuvone, P.
Regulation of arylalkylamine N-acetyltransferase (AANAT) in the retina
Chronobiol. Int.
23
381-391
2006
Macaca mulatta (O97756), Gallus gallus (P79774), Rattus norvegicus (Q64666), Rattus norvegicus Fischer (Q64666)
Manually annotated by BRENDA team
Pozdeyev, N.; Taylor, C.; Haque, R.; Chaurasia, S.S.; Visser, A.; Thazyeen, A.; Du, Y.; Fu, H.; Weller, J.; Klein, D.C.; Iuvone, P.M.
Photic regulation of arylalkylamine N-acetyltransferase binding to 14-3-3 proteins in retinal photoreceptor cells
J. Neurosci.
26
9153-9161
2006
Gallus gallus
Manually annotated by BRENDA team
Rosiak, J.; Zawilska, J.B.
Near-ultraviolet light perceived by the retina generates the signal suppressing melatonin synthesis in the chick pineal gland-an involvement of NMDA glutamate receptors
Neurosci. Lett.
379
214-217
2005
Gallus gallus (P79774), Gallus gallus
Manually annotated by BRENDA team
Iuvone, P.M.; Tosini, G.; Pozdeyev, N.; Haque, R.; Klein, D.C.; Chaurasia, S.S.
Circadian clocks, clock networks, arylalkylamine N-acetyltransferase, and melatonin in the retina
Prog. Retin. Eye Res.
24
433-456
2005
Gallus gallus, Rattus norvegicus, Xenopus laevis
Manually annotated by BRENDA team