Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.3.1.71 - glycine N-benzoyltransferase and Organism(s) Homo sapiens and UniProt Accession Q6IB77

for references in articles please use BRENDA:EC2.3.1.71
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Not identical with EC 2.3.1.13, glycine N-acyltransferase or EC 2.3.1.68, glutamine N-acyltransferase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q6IB77
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
benzoyl-coa:glycine n-acyltransferase, aralkyl-coa:glycine n-acyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aralkyl-CoA:glycine N-acyltransferase
-
-
-
-
benzoyl CoA-amino acid N-acyltransferase
-
-
-
-
benzoyl-CoA:glycine N-acyltransferase
-
-
-
-
benzoyltransferase, glycine
-
-
-
-
GlyAT
-
-
glycine N-acyltransferase
GNAT
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
benzoyl-CoA:glycine N-benzoyltransferase
Not identical with EC 2.3.1.13, glycine N-acyltransferase or EC 2.3.1.68, glutamine N-acyltransferase
CAS REGISTRY NUMBER
COMMENTARY hide
71567-07-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-aminobenzoyl-CoA + glycine
CoA + N-(4-aminobenzoyl)glycine
show the reaction diagram
-
-
-
?
benzoyl-CoA + glycine
CoA + N-benzoylglycine
show the reaction diagram
benzoyl-CoA + L-glutamine
CoA + N-benzoyl-L-glutamine
show the reaction diagram
24% of the rate with glycine and benzoyl-CoA
-
-
?
phenylacetyl-CoA + glycine
CoA + N-phenylacetylglycine
show the reaction diagram
22% of the rate with glycine and benzoyl-CoA
-
-
?
phenylacetyl-CoA + L-glutamine
CoA + N-phenylacetyl-L-glutamine
show the reaction diagram
20% of the rate with glycine and benzoyl-CoA
-
-
?
salicyl-CoA + glycine
CoA + salicyluric acid
show the reaction diagram
-
-
-
?
benzoyl-CoA + alanine
CoA + N-benzoylalanine
show the reaction diagram
-
5% of the activity with Gly
-
-
?
benzoyl-CoA + glutaminic acid
CoA + N-benzoylglutamic acid
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + glycine
CoA + hippurate
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + glycine
CoA + N-benzoylglycine
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + serine
CoA + N-benzoylserine
show the reaction diagram
-
0.88% of the activity with Gly
-
-
?
heptanoyl-CoA + glycine
CoA + N-heptanoylglycine
show the reaction diagram
-
9.5% of the activity with benzoyl-CoA
-
-
?
indoleacetyl-CoA + glycine
CoA + N-indoleacetylglycine
show the reaction diagram
-
-
-
-
?
isovaleryl-CoA + glycine
CoA + N-isovalerylglycine
show the reaction diagram
-
9.1% of the activity with benzoyl-CoA
-
-
?
n-butyryl-CoA + glycine
CoA + N-butyrylglycine
show the reaction diagram
-
28% of the activity with benzoyl-CoA
-
-
?
salicyl-CoA + glycine
CoA + salicyluric acid
show the reaction diagram
-
22% of the activity with benzoyl-CoA
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + glycine
CoA + hippurate
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021 - 0.071
benzoyl-CoA
997
Ala
-
-
0.049 - 15.2
benzoyl-CoA
6.4
Gly
-
-
5 - 21
glycine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLYAT_HUMAN
296
0
33924
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
x * 27000, SDS-PAGE
30500
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 27000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
molecular model containing coenzyme A. The inactivity of the R199C variant could be due to the substitution of the highly conserved Arg199 and destabilisation of an alpha-loop-alpha motif which is important for substrate binding
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E229Q
single nucleotide polymorphism, inactive
F168L
single nucleotide polymorphism, decreased activity compared to wild-type
K16N
single nucleotide polymorphism, enzymic activities similar to wild-type
N156S
single nucleotide polymorphism, more active than wild-type
R131H
single nucleotide polymorphism, enzymic activities similar to wild-type
R199C
single nucleotide polymorphism, inactive
S17T
single nucleotide polymorphism, enzymic activities similar to wild-type
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nickel-affinity column chromatography
-
partial
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
isoform Glyat expression is suppressed in all hepatocellular carcinomas, but not in other liver diseases. Glyat repression in cancerous cells in the liver is controlled at the transcriptional level
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Van der Westhuizen, F.H.; Pretorius, P.J.; Erasmus, E.
The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase
J. Biochem. Mol. Toxicol.
14
102-109
2000
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Kelley, M.; Vessey, D.A.
Characterization of the acyl-CoA:amino acid N-acyltransferases from primate liver mitochondria
J. Biochem. Toxicol.
9
153-158
1994
Homo sapiens
Manually annotated by BRENDA team
Matsuo, M.; Terai, K.; Kameda, N.; Matsumoto, A.; Kurokawa, Y.; Funase, Y.; Nishikawa, K.; Sugaya, N.; Hiruta, N.; Kishimoto, T.
Designation of enzyme activity of glycine-N-acyltransferase family genes and depression of glycine-N-acyltransferase in human hepatocellular carcinoma
Biochem. Biophys. Res. Commun.
420
901-906
2012
Homo sapiens (Q6IB77)
Manually annotated by BRENDA team
Van der Sluis, R.; Badenhorst, C.; Van der Westhuizen, F.; Van Dijk, A.
Characterisation of the influence of genetic variations on the enzyme activity of a recombinant human glycine N-acyltransferase
Gene
515
447-453
2013
Homo sapiens (Q6IB77)
Manually annotated by BRENDA team
van der Sluis, R.; Badenhorst, C.P.; Erasmus, E.; van Dyk, E.; van der Westhuizen, F.H.; van Dijk, A.A.
Conservation of the coding regions of the glycine N-acyltransferase gene further suggests that glycine conjugation is an essential detoxification pathway
Gene
571
126-134
2015
Homo sapiens (Q6IB77)
Manually annotated by BRENDA team
Nortje, C.; van der Sluis, R.; van Dijk, A.A.; Erasmus, E.
The use of p-aminobenzoic acid as a probe substance for the targeted profiling of glycine conjugation
J. Biochem. Mol. Toxicol.
30
136-147
2015
Homo sapiens (Q6IB77)
Manually annotated by BRENDA team
van der Sluis, R.; Ungerer, V.; Nortje, C.; A van Dijk, A.; Erasmus, E.
New insights into the catalytic mechanism of human glycine N-acyltransferase
J. Biochem. Mol. Toxicol.
31
e21963
2017
Homo sapiens
Manually annotated by BRENDA team