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Information on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O22975

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IUBMB Comments
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O22975
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
abhd5, agpat2, lpaat, lysophosphatidic acid acyltransferase, lpaat-beta, agpat1, 1-acylglycerol-3-phosphate o-acyltransferase, agpat3, lpa acyltransferase, agpat4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase
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1-acyl-sn-glycero-3-phosphate acyltransferase
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1-acyl-sn-glycerol 3-phosphate acyltransferase
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1-acyl-sn-glycerol-3-phosphate acyltransferase
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1-acylglycero-3-phosphate acyltransferase
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1-acylglycerolphosphate acyltransferase
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1-acylglycerophosphate acyltransferase
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acyltransferase, 1-acylglycerol phosphate
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endoplasmic reticulum-located LPAT
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ER-located LPAT
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lysophosphatidic acid-acyltransferase
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lysophosphatidyl acyltransferase
additional information
the enzyme belongs to the alpha/beta-hydrolase family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
51901-16-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
1-oleoyl-sn-glycerol-3-phosphate + oleoyl-CoA
CoA + 1,2-oleoyl-sn-glycerol-3-phosphate
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycerol-3-phosphate + palmitoyl-CoA
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol-3-phosphate
show the reaction diagram
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-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
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pivotal enzyme controlling the metabolic flow of lysophosphatidic acid into different phosphatidic acids in diverse tissues, enzyme is essential in embryo development
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-
?
acyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
pivotal enzyme controlling the metabolic flow of lysophosphatidic acid into different phosphatidic acids in diverse tissues, enzyme is essential in embryo development
-
-
?
acyl-CoA + 1-oleoyl-lysophosphatidic acid
CoA + 1-oleoyl-2-acyl-lysophosphatidic acid
show the reaction diagram
-
-
-
?
additional information
?
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
At4g24160; gene At4g24160
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the endoplasmic reticulum-located LPAT2 is essential for female but not male gametophyte development in Arabidopsis thaliana
Manually annotated by BRENDA team
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
LPAAT is a crucial enzyme controlling the metabolic flow of lysophosphatidic acid into the pool of phosphatidic acid, which plays a key role in many physiological aspects, such as cell signaling, cell polarity, and apoptotic signaling cascades in higher eukaryotes. Phosphatidic acid specifically regulates the genes involved in modulating cell shape and organization
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LPAAT_ARATH
418
0
46526
Swiss-Prot
Chloroplast (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged At4g24160 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene At4g24160, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
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gene LPAAT2, transient expression in leaves of Nicotiana benthamiana via Agrobacterium tumefaciens strain AGL1 transfection method
gene LPAT1, genetic structure, phylogenetic tree
gene LPAT2, genetic structure, LPAT2-cDNA driven by an LPAT2 promoter functionally complements insertion mutant lpat2 in transformed heterozygous mutants to produce the lpat2/lpat2 genotype, overview, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping
gene LPAT3, genetic structure, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping
gene LPAT4, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme
gene LPAT5, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, H.U.; Huang, A.H.C.
Plastid lysophosphatidyl acyltransferase is essential for embryo development in Arabidopsis
Plant Physiol.
134
1206-1216
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Kim, H.U.; Li, Y.; Huang, A.H.
Ubiquitous and endoplasmic reticulum-located lysophosphatidyl acyltransferase, LPAT2, is essential for female but not male gametophyte development in Arabidopsis
Plant Cell
17
1073-1089
2005
Arabidopsis thaliana (Q8GXU8), Arabidopsis thaliana (Q8L4Y2), Arabidopsis thaliana (Q8LG50), Arabidopsis thaliana (Q9LHN4), Arabidopsis thaliana (Q9SYC8), Arabidopsis thaliana
Manually annotated by BRENDA team
Ghosh, A.K.; Chauhan, N.; Rajakumari, S.; Daum, G.; Rajasekharan, R.
At4g24160, a soluble acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase
Plant Physiol.
151
869-881
2009
Arabidopsis thaliana (O22975)
Manually annotated by BRENDA team
Shrestha, P.; Hussain, D.; Mulder, R.J.; Taylor, M.C.; Singh, S.P.; Petrie, J.R.; Zhou, X.R.
Increased DHA production in seed oil using a selective lysophosphatidic acid acyltransferase
Front. Plant Sci.
9
1234
2018
Mortierella alpina, Yarrowia lipolytica (A0A1D8NJ27), Micromonas pusilla (C1MQM1), Saccharomyces cerevisiae (P33333), Limnanthes alba (Q42868), Arabidopsis thaliana (Q8LG50), Brassica napus (Q9LLY4), Emiliania huxleyi (R1BZP3), Emiliania huxleyi
Manually annotated by BRENDA team