Information on EC 2.3.1.5 - arylamine N-acetyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.3.1.5
-
RECOMMENDED NAME
GeneOntology No.
arylamine N-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and inactivation
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and characterization of active site
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and characterization of active site
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
a conserved active site loop is involved in substrate recognition, structure analysis, Ser125 in NAT2 is proximal to the catalytic triad and faces a passageway to the catalytic core
P11245
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
Cys69, His108, and Asp125 form the catalytic triad
A2V7M5
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
molecular mechanism of arylamine acetylation by the catalytic triad of NAT1, overview, the catalytic triad is formed by Cys68, His107, and Asp122
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping pong reaction mechanism, kinetic mechanism, overview
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping pong reaction mechanism, the enzyme contains a strictly conserved a Cys-His-Asp catalytic triad, acetylation of the hamster NAT2 is dependent on a thiolate-imidazolium ion pair, Cys-S--His-ImH+, and not a general acid-base catalysis, a deacetylation mechanism dominated by nucleophilic attack of the thiol ester for arylamines
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
structure-function analysis, Cys68-His107-Asp122 catalytic triad
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
it is shown that the formation of a thiolate-imidazolium ion pair by Cys68 and His107 is involved in the catylytic mechanism. Asp122 is required for optimal catalysis and structural stability. Enzyme deacetylation step proceeds via nucleophilic attack for acceptor amines with pKa values less than 5.5 and by deprotonation of a tetrahedral intermediate for amines with pKa values greater than 5.5
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
steady-state kinetic analysis of TBNAT reveals that the enzyme catalyzes the reaction via a bi-bi ping-pong kinetic mechanism: enzyme initially reacts with AcCoA to generate the first product, CoA, and the acetyl-enzyme intermediate (E-Ac). The amine binds and reacts with E-Ac to generate the second product, acetylated amine, and regenerate the free enzyme. The pH dependence of the kinetic parameters reveals that one enzyme group must be deprotonated for optimal catalytic activity and that two amino acid residues at the active site of the free enzyme are involved in binding and/or catalysis. Solvent kinetic isotope effects suggest that proton transfer steps are not rate-limiting in the overall reaction for substituted aniline substrates but become rate-limiting when poor hydrazide substrates are used
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
residue Y190 is critical for maximizing the acetylation rate of NAT2 and the transacetylation rate of substrate p-aminobenzoic acid. Y190 also plays an important role in determining the pKa of the active site Cys during acetylation, as well as the pH versus the rate profile for transacetylation
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
Rattus norvegicus Sprague-Dawley, Mus musculus C57BL/67
-
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
Cys69, His108, and Asp125 form the catalytic triad
Streptomyces griseus IFO13350
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Caffeine metabolism
-
-
Drug metabolism - other enzymes
-
-
grixazone biosynthesis
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
Nitrotoluene degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:arylamine N-acetyltransferase
Wide specificity for aromatic amines, including serotonin; also catalyses acetyl-transfer between arylamines without CoA.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-naphthylamine N-acetyltransferase
-
-
-
-
4-aminobiphenyl N-acetyltransferase
-
-
-
-
acetyl CoA-arylamine N-acetyltransferase
-
-
-
-
acetyltransferase, 2-naphthylamine N-
-
-
-
-
acetyltransferase, 4-aminobiphenyl
-
-
-
-
acetyltransferase, arylamine
-
-
-
-
acetyltransferase, p-aminosalicylate N-
-
-
-
-
acetyltransferase, procainamide N-
-
-
-
-
acetyltransferase, serotonin N-
-
-
-
-
arylamine acetylase
-
-
-
-
arylamine acetyltransferase
-
-
-
-
beta-naphthylamine N-acetyltransferase
-
-
-
-
indoleamine N-acetyltransferase
-
-
-
-
N-acetyltransferase
-
-
-
-
NAT
-
-
-
-
NAT1
-
-
-
-
NAT2
-
-
-
-
p-aminosalicylate N-acetyltransferase
-
-
-
-
serotonin acetyltransferase
-
-
-
-
serotonin N-acetyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9027-33-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Achromobacter xylosoxidans 71.32
strain 71.32
-
-
Manually annotated by BRENDA team
strain 76.14
-
-
Manually annotated by BRENDA team
Aeromonas hydrophila 76.14
strain 76.14
-
-
Manually annotated by BRENDA team
duck
-
-
Manually annotated by BRENDA team
; BanatA
UniProt
Manually annotated by BRENDA team
; BanatB
UniProt
Manually annotated by BRENDA team
; BanatC
UniProt
Manually annotated by BRENDA team
distribution among soil microorganisms, overview
-
-
Manually annotated by BRENDA team
strain 10-L-2
-
-
Manually annotated by BRENDA team
Bacillus cereus 10-L-2
strain 10-L-2
-
-
Manually annotated by BRENDA team
strain 100
-
-
Manually annotated by BRENDA team
Bacteroides sp. 100
strain 100
-
-
Manually annotated by BRENDA team
no activity in Canis lupus familiaris
-
-
Manually annotated by BRENDA team
strain 82.89
-
-
Manually annotated by BRENDA team
Citrobacter amalonaticus 82.89
strain 82.89
-
-
Manually annotated by BRENDA team
strain 104553
-
-
Manually annotated by BRENDA team
Citrobacter farmeri 104553
strain 104553
-
-
Manually annotated by BRENDA team
strain 57.32
-
-
Manually annotated by BRENDA team
Citrobacter freundii 57.32
strain 57.32
-
-
Manually annotated by BRENDA team
strain 82.94
-
-
Manually annotated by BRENDA team
Citrobacter koseri 82.94
strain 82.94
-
-
Manually annotated by BRENDA team
insect pathogen fungus
-
-
Manually annotated by BRENDA team
strain N5-36
-
-
Manually annotated by BRENDA team
Enterobacter aerogenes N5-36
strain N5-36
-
-
Manually annotated by BRENDA team
strain 54.8; strain K-12 MG1655
-
-
Manually annotated by BRENDA team
Escherichia coli 54.8
strain 54.8
-
-
Manually annotated by BRENDA team
Escherichia coli K-12 MG1655
strain K-12 MG1655
-
-
Manually annotated by BRENDA team
strain 43579
-
-
Manually annotated by BRENDA team
Helicobacter pylori 43579
strain 43579
-
-
Manually annotated by BRENDA team
genetic polymorphism
-
-
Manually annotated by BRENDA team
human
-
-
Manually annotated by BRENDA team
isoform NAT 1
SwissProt
Manually annotated by BRENDA team
isoform NAT 2
SwissProt
Manually annotated by BRENDA team
isoform NAT2
SwissProt
Manually annotated by BRENDA team
isozyme NAT1
-
-
Manually annotated by BRENDA team
isozyme NAT1; gene NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT1; isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2
-
-
Manually annotated by BRENDA team
isozyme NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
NAT1
-
-
Manually annotated by BRENDA team
NAT1 and NAT2
-
-
Manually annotated by BRENDA team
NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
recombinant NAT2, several genetic variants
-
-
Manually annotated by BRENDA team
two isozymes NAT1, NAT2
-
-
Manually annotated by BRENDA team
strain 103434
-
-
Manually annotated by BRENDA team
Klebsiella oxytoca 103434
strain 103434
-
-
Manually annotated by BRENDA team
strain 82.91
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae 82.91
strain 82.91
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae subsp. ozaenae 52.211
strain 52.211
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae subsp. rhinoscleromatis 52.210
strain 52.210
-
-
Manually annotated by BRENDA team
polymorphic allele Nat2*1; polymorphic allele Nat2*2
Uniprot
Manually annotated by BRENDA team
enzyme form NAT2
-
-
Manually annotated by BRENDA team
isozyme NAT1; isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
recombinant NAT1, NAT2
-
-
Manually annotated by BRENDA team
recombinant protein, fusion with dihydrofolate reductase
-
-
Manually annotated by BRENDA team
recombinant protein, NAT1
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
strain A.231
-
-
Manually annotated by BRENDA team
Morganella morganii A.231
strain A.231
-
-
Manually annotated by BRENDA team
fast acetylator strain C57BL6, slow acetylator strain A/J
-
-
Manually annotated by BRENDA team
isoform NAT1
-
-
Manually annotated by BRENDA team
isozyme NAT1; male mice, isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2; male mice, isozyme NAT2
SwissProt
Manually annotated by BRENDA team
housefly
-
-
Manually annotated by BRENDA team
K-10, str. 104
-
-
Manually annotated by BRENDA team
; BCG Pasteur, AF2122/97
-
-
Manually annotated by BRENDA team
; strain BCG Pasteur
-
-
Manually annotated by BRENDA team
NAT gene is essential for survival of Mycobacterium bovis
-
-
Manually annotated by BRENDA team
strain Bacille Calmette-Guerin
-
-
Manually annotated by BRENDA team
strain BCG, gene nat
-
-
Manually annotated by BRENDA team
Mycobacterium bovis Bacille
strain Bacille Calmette-Guerin
-
-
Manually annotated by BRENDA team
; CDC1551, Haarlem, F11, C, H73Rv
-
-
Manually annotated by BRENDA team
no activity in Canis familiaris
-
-
-
Manually annotated by BRENDA team
new Zealand white
-
-
Manually annotated by BRENDA team
new Zealand white; rapid acetylator type III/J
-
-
Manually annotated by BRENDA team
slow and fast acetylator phenotype
-
-
Manually annotated by BRENDA team
strain 103286
-
-
Manually annotated by BRENDA team
Pasteurella multocida 103286
strain 103286
-
-
Manually annotated by BRENDA team
Plesiomonas shigelloides 63.5
strain 63.5
-
-
Manually annotated by BRENDA team
strain 58.60
-
-
Manually annotated by BRENDA team
Proteus vulgaris 58.60
strain 58.60
-
-
Manually annotated by BRENDA team
Providencia alcalifaciens 82.90
strain 82.90
-
-
Manually annotated by BRENDA team
strain 100720
-
-
Manually annotated by BRENDA team
strain ATCC 27853
-
-
Manually annotated by BRENDA team
strain PAO1, serotype O5, gene wbpD
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 100720
strain 100720
-
-
Manually annotated by BRENDA team
hepatocyte primary culture
-
-
Manually annotated by BRENDA team
isozyme Nat3; inbred strains F344, WKY, and SPRD, isozymes Nat1, Nat2, and Nat3
SwissProt
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
RHA1
-
-
Manually annotated by BRENDA team
Rhodococcus RHA1
RHA1
-
-
Manually annotated by BRENDA team
strain YG1024, strain TA98 1,8/DNP, strain TA1538, strain TA98, strain 60.62
-
-
Manually annotated by BRENDA team
strain 103235
-
-
Manually annotated by BRENDA team
Serratia marcescens 103235
strain 103235
-
-
Manually annotated by BRENDA team
strain 82.48
-
-
Manually annotated by BRENDA team
Shigella flexneri 82.48
strain 82.48
-
-
Manually annotated by BRENDA team
strain IFO13350, gene natA
SwissProt
Manually annotated by BRENDA team
Streptomyces griseus IFO13350
strain IFO13350, gene natA
SwissProt
Manually annotated by BRENDA team
strain 54.315
-
-
Manually annotated by BRENDA team
Vibrio cholerae 54.315
strain 54.315
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
increasing acetylation by introduction of the human NAT1 transgene is protective against sporadic dilantin-induced orofacial clefting defects in the mouse A/J strain
physiological function
-
knock-down of enzyme by lentiviral shRNA reduces the invasion potential of MDA-MB-231 cells. Enzymic activity may be important in breast cancer growth and metastasis
physiological function
-
knockdown of N-acetyltransferase by siRNA results in downregulation of thymidylate synthase mRNA expression and induced apoptosis. N-acetyltransferase overexpression facilitates cell proliferation independent of the presence of 5-fluorouracil
physiological function
-
knockout of NAT2 significantly decreases teratogen-induced orofacial clefting in the A/J strain, but not in the C57BL/6J strain
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + 2-aminofluorene
4-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 2-aminofluorene
4-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
substrate of isozyme NAT1, very low activity with isozyme NAT2
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
P50292
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
P50293
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
a sunscreen additive, substrate of isozyme NAT1, very low activity with isozyme NAT2
-
-
?
4-nitrophenyl acetate + 4-aminobenzoyl glutamic acid
4-nitrophenol + N-acetyl-4-aminobenzoyl glutamic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoyl glutamic acid
4-nitrophenol + N-acetyl-4-aminobenzoyl glutamic acid
show the reaction diagram
P50293
-
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
a tobacco smoke carcinogen, substrate of isozyme NAT1, very low activity with isozyme NAT2
-
-
?
4-nitrophenyl acetate + 4-anisidine
4-nitrophenol + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-nitroaniline
4-nitrophenol + N-acetyl-4-nitroaniline
show the reaction diagram
P50293
-
-
-
?
4-nitrophenyl acetate + 5-aminosalicylic acid
4-nitrophenol + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 5-aminosalicylic acid
4-nitrophenol + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + aniline
4-nitrophenol + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + anisidine
4-nitrophenol + N-acetyl-anisidine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + anisidine
4-nitrophenol + N-acetyl-anisidine
show the reaction diagram
P50293
-
-
-
?
4-nitrophenyl acetate + hydralazine
4-nitrophenol + N-acetyl-hydralazine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + isoniazid
4-nitrophenol + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + (1-methyl-5-piperazin-1-yl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7-yl)-(5-methyl-pyridin-2-yl)-amine
CoA + ?
show the reaction diagram
-
i.e. UK-469,413. Acetylation by isozyme NAT2 in liver cytosol to N-acetylpiperazine metabolite
-
-
?
acetyl-CoA + (1-methyl-5-piperazin-1-yl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7-yl)-(5-methyl-pyridin-2-yl)-amine
CoA + ?
show the reaction diagram
-
i.e. UK-469,413. Rapid acetylation in liver cytosol to N-acetylpiperazine metabolite
-
-
?
acetyl-CoA + 2,3-dimethylaniline
CoA + N-acetyl-2,3-dimethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2,4-dimethylaniline
CoA + N-acetyl-2,4-dimethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2,5-dimethylaniline
CoA + N-acetyl-2,5-dimethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-(4-aminobenzamido)pyridine
CoA + 2-(4-acetylamidobenzamido)pyridine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + 2-acetylamino-4-chlorophenol
show the reaction diagram
A2V7M5
-
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + N-(5-chloro-2-hydroxyphenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + N-acetyl-2-amino-4-chlorophenol
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + 2-acetylamino-4-methylphenol
show the reaction diagram
A2V7M5
best substrate
-
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + N-(2-hydroxy-5-methylphenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + N-acetyl-2-amino-4-methylphenol
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetylanthranilate
show the reaction diagram
-
i.e. anthranilate, ir
-
ir
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetyl-2-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetyl-2-aminobenzoic acid
show the reaction diagram
-
relative activity: 0%
-
-
?
acetyl-CoA + 2-aminobiphenyl
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
N-acetyltransferase 2
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
NAT1, poor substrate
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
interactions and substrate binding structure, overview
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
high specific activity
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
mutant Y71F, 128% of wild-type activity, mutant M209T, 62% of wild-type activity
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + 2-acetylaminophenol
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
A2V7M5
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-(2-hydroxyphenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-acetyl-2-aminophenol
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-acetyl-2-aminophenol
show the reaction diagram
-
relative activity: 36%, relative activity: 72%
-
-
?
acetyl-CoA + 2-chloroaniline
CoA + N-acetyl-2-chloroaniline
show the reaction diagram
-
relative activity: 12%, relative activity: 18%
-
-
?
acetyl-CoA + 2-ethylaniline
CoA + N-acetyl-2-ethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-methylaniline
CoA + N-acetyl-2-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-nitroaniline
CoA + N-acetyl-2-nitroaniline
show the reaction diagram
-
relative activity: 0%, relative activity: 3%
-
-
?
acetyl-CoA + 2-phenylendiamine
CoA + ?
show the reaction diagram
-
relative activity: 80%, relative activity: 90%
-
-
?
acetyl-CoA + 2-toluidine
CoA + N-acetyl-2-toluidine
show the reaction diagram
-
relative activity: 0%
-
-
?
acetyl-CoA + 3,4-dichloraniline
CoA + N-acetyl-3,4-dichloraniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
mutant Y71F, 194% of wild-type activity, mutant M209T, 88% of wild-type activity
-
-
?
acetyl-CoA + 3,4-dimethylaniline
CoA + N-acetyl-3,4-dimethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,5-dimethyl-4-nitroaniline
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,5-dimethylaniline
CoA + N-acetyl-3,5-dimethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,5-dimethylaniline
CoA + N-acetyl-3,5-dimethylaniline
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
A2V7M5
an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
Streptomyces griseus IFO13350
A2V7M5
an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzenesulfonic acid
CoA + 3-acetylamino-4-hydroxybenzenesulfonic acid
show the reaction diagram
A2V7M5
-
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
A2V7M5
best substrate, an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
-
an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
Streptomyces griseus IFO13350
A2V7M5
best substrate
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-(acetylamino)-4-hydroxybenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + N-acetyl-3-amino-4-hydroxybenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
-
relative activity: 105%, relative activity: 122%
-
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
Bacillus cereus 10-L-2
-
relative activity: 105%, relative activity: 122%
-
-
?
acetyl-CoA + 3-aminophenol
CoA + N-acetyl-3-aminophenol
show the reaction diagram
-
relative activity: 33%, relative activity: 56%
-
-
?
acetyl-CoA + 3-chloraniline
CoA + N-acetyl-3-chloroaniline
show the reaction diagram
-
relative activity: 48%
-
-
?
acetyl-CoA + 3-ethylaniline
CoA + N-acetyl-3-ethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3-ethylaniline
CoA + N-acetyl-3-ethylaniline
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 3-nitroaniline
CoA + N-acetyl-3-nitroaniline
show the reaction diagram
-
relative activity: 2%, relative activity: 6%
-
-
?
acetyl-CoA + 3-phenylendiamine
CoA + ?
show the reaction diagram
-
relative activity: 0%
-
-
?
acetyl-CoA + 3-toluidine
CoA + N-acetyl-3-toluidine
show the reaction diagram
-
relative activity: 21%, relative activity: 28%
-
-
?
acetyl-CoA + 4,4'-methylenebis(2-chloroaniline)
CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline)
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 4,4'-methylenedianiline
CoA + N-acetyl-4,4'-methylenedianiline
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + 4-acetylamino-3-hydroxybenzoic acid
show the reaction diagram
A2V7M5
low activity
-
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + 4-(acetylamino)-3-hydroxybenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + N-acetyl-4-amino-3-hydroxybenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-amino-4'-nitroazobenzene
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoacetanilide
CoA + N-acetyl-4-aminoacetanilide
show the reaction diagram
-
relative activity: 0%
-
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
poor
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
N-acetyltransferase 1
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
the catalytic mechanism depends on the formation of a thiolate-imidazolium ion pair (Cys-S-)-(His-ImH+)
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
Enterobacter aerogenes N5-36
-
poor
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
P50294, P50295
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
P50293
preferred substrate
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
isozyme NAT1
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
high specific activity
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
relative activity: 19%, relative activity: 26%
-
-
?
acetyl-CoA + 4-aminobenzoic acid
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-(acetylamino)benzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamate
CoA + 4-acetylaminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamate
CoA + N-acetyl-4-aminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamic acid
CoA + 4-acetylaminobenzoyl-L-glutamic acid
show the reaction diagram
P50293
-
-
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2
-
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + 4-acetylaminobiphenyl
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + N-acetyl-4-aminobiphenyl
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminofluorene
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminophenol
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminophenol
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-aminophenol
CoA + N-acetyl-4-aminophenol
show the reaction diagram
-
relative activity: 48%, relative activity: 94%
-
-
?
acetyl-CoA + 4-aminopyridine
?
show the reaction diagram
-
very low activity
-
-
?
acetyl-CoA + 4-aminopyridine
?
show the reaction diagram
O86309
very low activity
-
-
?
acetyl-CoA + 4-aminopyridine
CoA + N-acetyl-4-aminopyridine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + 4-aminosalicylate
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
N-acetyltransferase 1
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
Mus musculus C57BL/67
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
high specific activity
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
relative activity: 100%, relative activity: 90%
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
mutant Y71F, 17% of wild-type activity, mutant M209T, 128% of wild-type activity
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
mutant Y71F, 860% of wild-type activity, mutant M209T, 673% of wild-type activity
-
-
?
acetyl-CoA + 4-anisidine
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromaniline
CoA + N-acetyl-4-bromaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-(4-bromophenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-acetyl-4-bromoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-acetyl-4-bromoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-butoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
relative activity: 18%, relative activity: 21%
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
mutant Y71F, 174% of wild-type activity, mutant M209T, 122% of wild-type activity
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-(4-chlorophenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-(4-chlorophenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N'-acetyl-(4-chlorobenzo)-hydrazide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N-acetyl-4-chlorobenzoic hydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N-acetyl-4-chlorobenzoic hydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-dimethylaminobenzaldehyde
CoA + 5-acetyl-4-dimethylaminobenzaldehyde
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethylaniline
CoA + 4-ethylacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethylaniline
CoA + N-acetyl-4-ethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-fluoroaniline
CoA + N-(4-fluorophenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-fluoroaniline
CoA + N-acetyl-4-fluoroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
O86309
best substrate
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hydroxybenzhydrazide
CoA + N'-acetyl-4-hydroxybenzohydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hydroxybenzhydrazide
CoA + N-acetyl-4-hydroxybenzhydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
mutant Y71F, 196% of wild-type activity, mutant M209T, 83% of wild-type activity
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-(4-iodophenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methoxyaniline
CoA + N-acetyl-4-methoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + N-acetyl-4-methoxyphenylhydrazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-(4-methylphenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
P50293
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
relative activity: 0%
-
-
?
acetyl-CoA + 4-nitrobenzalhydrazone
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenylenediamine
CoA + 4-aminoacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-toluidine
CoA + N-acetyl-4-toluidine
show the reaction diagram
-
relative activity: 40%, relative activity: 80%
-
-
?
acetyl-CoA + 4-trifluoromethylaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-trifluoromethylaniline
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 4-trifluoromethylaniline
CoA + N-acetyl-4-trifluoromethylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + 5-aminosalicylate
?
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + 5-aminosalicylate
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
high specific activity
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
relative activity: 510%, relative activity: 550%
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
mutant Y71F, 147% of wild-type activity, mutant M209T, 21% of wild-type activity
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
A2V7M5
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
poor substrate
-
-
?
acetyl-CoA + 8-aminoisoindolo (1,2-b)quinazolin-12(10H)-one
?
show the reaction diagram
-
batricylin, an antitumor agent, is shown to be a substrate for NAT2
-
-
-
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
involved in metabolism of drugs and endogenous substances containing an amine or hydrazine group
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
pineal gland: inducible
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
expression of NAT1 in muscle cells may be an important factor in the detoxification/activation process because of the potential involvement of the muscle in the pharmacokinetics of many xenobiotics
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
important role in detoxification and metabolic activation of a variety of aromatic xenobiotics, including numerous carcinogens. Cellular generation of peroxinitrite may contribute to carcinogenesis and tumor progression by weakening key cellular defense enzymes such as NAT1
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
constitutive enzyme, pineal gland: inducible
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50293
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P18440
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50294, P50295
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
O86309
endogenous role of the enzyme in the protection of bacteria from aromatic and lipophilic toxins
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
catalytic pathway, overview
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245
NAT2 is responsible for the biotransformation of numerous arylamine drugs and carcinogens
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are important enzymes involved in the metabolic activation of aromatic and heterocyclic amines
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245, P18440
the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245, P18440
the enzyme catalyzes the N-acetylation of arylamines and hydrazines and O-acetylation of N-hydroxy-arylamines and heterocyclic amines
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-hydroxyarylamine
CoA + an N-acetoxyarylamine
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetylaniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
Enterobacter aerogenes N5-36
-
-
-
?
acetyl-CoA + aniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
relative activity: 12%, relative activity: 24%
-
-
?
acetyl-CoA + aniline
CoA + N-phenylacetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
P50293
-
-
-
?
acetyl-CoA + anisidine
CoA + N-(4-methoxyphenyl)acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + benzidine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + benzidine
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + benzidine
CoA + N-acetyl-benzidine
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + benzoic acid hydrazide
CoA + N'-acetylbenzohydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + benzoic acid hydrazide
CoA + N-acetyl-benzoic acid hydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + beta-naphthylamine
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + beta-naphthylamine
CoA + N-acetyl-beta-naphthylamine
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
poor substrate
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
not chicken pineal gland and brain
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
acetyl-CoA + butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + decylamine
CoA + N-acetyldecylamine
show the reaction diagram
-
-
-
-
-
acetyl-CoA + decylamine
CoA + N-acetyldecylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + diaminodiphenylsulfone
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + diaminodiphenylsulfone
monoacetyldiaminodiphenylsulfone + CoA
show the reaction diagram
-
i.e. dapsone, predominantly acetylated by NAT2
-
-
?
acetyl-CoA + hexylamine
CoA + N-acetylhexylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetylhydralazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + N'-phthalazin-1-ylacetohydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
low specific activity
-
-
?
acetyl-CoA + hydroxytyramine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
low specific activity
-
-
?
acetyl-CoA + isoniazid
CoA + acetylniazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N'-acetylbenzohydrazide
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
mutant Y71F, 102% of wild-type activity, mutant M209T, 229% of wild-type activity
-
-
?
acetyl-CoA + isoniazid
CoA + an N-acetylisoniazid
show the reaction diagram
P50294, P50295
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
P11245
inactivation of the anti-tubercular drug isoniazid by acetyltransfer
-
-
?
acetyl-CoA + isoniazid
CoA + N'-acetylpyridine-4-carbohydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
i.e. isonicotinyl hydrazide
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
isoniazide binds the enzyme prior to acetyl-CoA
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
Mycobacterium bovis Bacille
-
-
-
-
?
acetyl-CoA + long-chain primary amines
CoA + ?
show the reaction diagram
-
C6-C16
-
-
?
acetyl-CoA + methyl bis-2-chloroaniline
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N,N-1,4-phenylenediamine
CoA + ?
show the reaction diagram
-
relative activity: 100%
-
-
?
acetyl-CoA + N-(4-acetylaminobenzoyl)-L-glutamate
CoA + 4-acetylaminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-acetyl-N-hydroxy-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
Q80SX3
O-acetylation activity
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2, O-acetylation activity of NAT2
-
-
?
acetyl-CoA + naphthylamine
CoA + N-acetylnaphthylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + nicotinic acid hydrazide
CoA + N'-acetylpyridine-3-carbohydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + nicotinic acid hydrazide
CoA + N-acetyl-nicotinic acid hydrazide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + noradrenaline
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + normetanephrine
CoA + N-acetylnormetanephrine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + octylamine
CoA + N-acetyloctylamine
show the reaction diagram
-
-
-
-
-
acetyl-CoA + octylamine
CoA + N-acetyloctylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
Q7YRG5
poor activity
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
?
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + 4-(acetylamino)benzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
activity assay
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
in vitro N-acetylation assay
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoylglutamate
CoA + N-[(4-acetylamino)]benzoyl-L-glutamate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminosalicylate
CoA + N-acetyl-p-aminosalicylate
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-anisidine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-anisidine
?
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + p-anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-anisidine
CoA + p-methoxyacetanilide
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + p-anisidine
CoA + N-acetylanisidine
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + p-octopamine
CoA + N-acetyloctopamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-octopamine
CoA + N-acetyloctopamine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
i.e. 4-ethoxybenzenamine, best substrate
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
-
-
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
ie. 4-(2-aminoethyl)phenol
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
acetyl-CoA + peptide
CoA + Nalpha-acetylpeptide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + phenelzine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + phenylhydrazine
CoA + N-acetyl-phenylhydrazine
show the reaction diagram
-
low specific activity
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
N-acetyltransferase 2
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
Q7YRG5
lower activity
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl- procainamide
show the reaction diagram
-
low specific activity
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-procainamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
-
-
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
-
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + N-acetylsulfadiazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamerazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamerazine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
N-acetyltransferase 2
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
Bacillus cereus 10-L-2
-
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
-
very low activity
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
O86309
very low activity
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
P11245, P18440
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
isozyme NAT2
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
low specific activity
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
relative activity: 0%, relative activity: 46%
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-acetyl-sulfamethoxazole
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-acetyl-sulfamethoxazole
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
Q5YYQ3
-
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-[4-[(5-methylisoxazol-3-yl)sulfamoyl]phenyl]acetamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfanilamide
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfapyridine
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulphamethazine
CoA + N-acetylsulphamethazine
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + sulphamethoxazole
CoA + N-acetylsulphamethoxazole
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulphisoxazole
CoA + 4-acetylamino-N-(3,4-dimethylisoxazol-5-yl)-benzenesulfonamide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulphisoxazole
CoA + N-acetylsulphisoxazole
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tetradecylamine
CoA + N-acetyltetradecylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + thiazolsulfone
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
-
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
not chicken pineal gland and brain
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
CoA + ?
show the reaction diagram
-
much better acetyl donor for NAT1 than for NAT2
-
-
?
p-nitroacetanilide + aniline
p-nitroaniline + acetanilide
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + 2-aminofluorene
p-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + 5-aminosalicylic acid
p-nitrophenol + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + aniline
p-nitrophenol + N-acetyl-aniline
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + hydralazine
p-nitrophenol + N-acetyl-hydralazine
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + isoniazid
p-nitrophenol + N-acetyl-isoniazid
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + p-aminobenzoic acid
p-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + p-anisidine
p-nitrophenol + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + 2-aminofluorene
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + 5-aminosalicylic acid
4-nitrophenol + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + aniline
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + hydralazine
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + isoniazid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-aminobenzoic acid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-aminosalicylic acid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-anisidine
?
show the reaction diagram
-
-
-
-
?
sulphamethazine + acetyl-CoA
N-[4-[(4,6-dimethylpyrimidin-2-yl)sulfamoyl]phenyl]acetamide
show the reaction diagram
Q7YRG5
-
-
-
?
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
CoA + UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid
show the reaction diagram
-
WbpD is a putative 3-N-acetyltransferase that has been implicated in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, a precursor for the D-Man(2NAc3NAc)A residues in the B-band O antigen of Pseudomonas aeruginosa, the enzyme is involved in the catalysis of the fourth step by acting as an UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase, WbpD utilizes a direct-transfer reaction mechanism
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
the enzyme exhibits arylhydroxamic acid N,O-acetyltransferase activity
-
-
-
additional information
?
-
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
-
N-diacetyl cysteamine can replace acetyl-CoA with less than 10% efficiency
-
-
-
additional information
?
-
-
no substrate: o-nitroaniline
-
-
-
additional information
?
-
-
no substrate: o-nitroaniline
-
-
-
additional information
?
-
-
not butyryl-CoA, no substrate: glucosamine 6-phosphate
-
-
-
additional information
?
-
-
no substrate: phenylalanine, cyclohexylamine
-
-
-
additional information
?
-
-
no substrate: polyamines, amino acids, sphingosine bases, ethanolamine, C1-C4 primary amines, some secondary and tertiary amines
-
-
-
additional information
?
-
-
no substrate: 2-methyl-4-nitroaniline, 2,4-dinitroaniline, 4-nitrophenylhydrazine, carbopyridine
-
-
-
additional information
?
-
-
4-nitroacetanilide, 4-nitrophenylacetate can replace acetyl-CoA
-
-
-
additional information
?
-
-
propionyl-CoA can replace acetyl-CoA
-
-
-
additional information
?
-
-
no substrate: 2-amino-6-nitropyridine
-
-
-
additional information
?
-
-
acetylthiocholine can replace acetyl-CoA
-
-
-
additional information
?
-
-
no expression of NAT2
-
-
-
additional information
?
-
-
the enzyme is required for synthesis of mycolic acids and complex lipids
-
-
-
additional information
?
-
-
xenobiotic metabolizing enzyme. Catalyzes the biotransformation of primary aromatic amine or hydrazine drugs and carcinogens. In addition to genetically controlled interindividual variations in NAT1 activity, oxidative stress and cellular redox status may also regulate NAT1 activity
-
-
-
additional information
?
-
-
no acetylation of sulfamethazine and procainamide
-
-
-
additional information
?
-
P50292
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 is not increased in breast tumors compared to healthy tissue, the grade of malignancy is positively related with NAT1
-
-
-
additional information
?
-
P50292
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 is increased in breast tumors compared to healthy tissue, the grade of malignancy is negatively associated with NAT2
-
-
-
additional information
?
-
-
NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates
-
-
-
additional information
?
-
P50293
the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics
-
-
-
additional information
?
-
-
the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens
-
-
-
additional information
?
-
-
the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate
-
-
-
additional information
?
-
P18440
the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview
-
-
-
additional information
?
-
P50294, P50295
the isozymes catalyze the N-acetylation of aromatic amine and hydrazine drugs and carcinogens, after N-hydroxylation, they also catalyze the metabolic activation of N-hydroxy-arylamines via O-acetylation
-
-
-
additional information
?
-
-
variable expression of isozyme NAT1 due to genetic polymorphism, gene regulation or environmental influences is associated with individual susceptibility to various cancers, overview
-
-
-
additional information
?
-
P50293
4-aminobenzoic acid is the preferred substrate
-
-
-
additional information
?
-
-
NAT acceptor substrate specificity, overview
-
-
-
additional information
?
-
-
poor activity wit 2-toluidine and other arylamines linked to bladder cancer
-
-
-
additional information
?
-
-
poor activity with 2-toluidine and other arylamines linked to bladder cancer
-
-
-
additional information
?
-
Q80SX3
substrate specificities of isozymes Nat1-Nat3, overview
-
-
-
additional information
?
-
A2V7M5
substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview
-
-
-
additional information
?
-
-
enzyme follows a Ping Pong Bi Bi kinetic mechanism
-
-
-
additional information
?
-
-
mouse Nat2 is very similar to human NAT1 in substrate specificity
-
-
-
additional information
?
-
-
results confirm that substituted anilines are excellent substrates and that TBNAT is ineffective in hydrazide N-acetylation
-
-
-
additional information
?
-
Mus musculus C57BL/67
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
Streptomyces griseus IFO13350
A2V7M5
substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview
-
-
-
additional information
?
-
Enterobacter aerogenes N5-36
-
not butyryl-CoA, no substrate: glucosamine 6-phosphate, propionyl-CoA can replace acetyl-CoA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
a sunscreen additive
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
a tobacco smoke carcinogen
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + 2-acetylaminophenol
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
A2V7M5
-
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
A2V7M5
an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
A2V7M5
an intermediate of grixazone synthesis
-
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
A2V7M5
-
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
involved in metabolism of drugs and endogenous substances containing an amine or hydrazine group
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
pineal gland: inducible
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
expression of NAT1 in muscle cells may be an important factor in the detoxification/activation process because of the potential involvement of the muscle in the pharmacokinetics of many xenobiotics
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
important role in detoxification and metabolic activation of a variety of aromatic xenobiotics, including numerous carcinogens. Cellular generation of peroxinitrite may contribute to carcinogenesis and tumor progression by weakening key cellular defense enzymes such as NAT1
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50293
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P18440
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50294, P50295
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
Q80SX3
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
O86309
endogenous role of the enzyme in the protection of bacteria from aromatic and lipophilic toxins
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
catalytic pathway, overview
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245
NAT2 is responsible for the biotransformation of numerous arylamine drugs and carcinogens
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are important enzymes involved in the metabolic activation of aromatic and heterocyclic amines
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245, P18440
the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
constitutive enzyme, pineal gland: inducible
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-hydroxyarylamine
CoA + an N-acetoxyarylamine
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
O86309
-
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-acetyl-N-hydroxy-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
-
?
acetyl-CoA + peptide
CoA + Nalpha-acetylpeptide
show the reaction diagram
-
-
-
-
?
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
CoA + UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid
show the reaction diagram
-
WbpD is a putative 3-N-acetyltransferase that has been implicated in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, a precursor for the D-Man(2NAc3NAc)A residues in the B-band O antigen of Pseudomonas aeruginosa, the enzyme is involved in the catalysis of the fourth step by acting as an UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase
-
-
?
additional information
?
-
-
no expression of NAT2
-
-
-
additional information
?
-
-
the enzyme is required for synthesis of mycolic acids and complex lipids
-
-
-
additional information
?
-
-
xenobiotic metabolizing enzyme. Catalyzes the biotransformation of primary aromatic amine or hydrazine drugs and carcinogens. In addition to genetically controlled interindividual variations in NAT1 activity, oxidative stress and cellular redox status may also regulate NAT1 activity
-
-
-
additional information
?
-
P50292
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 is not increased in breast tumors compared to healthy tissue, the grade of malignancy is positively related with NAT1
-
-
-
additional information
?
-
P50292
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 is increased in breast tumors compared to healthy tissue, the grade of malignancy is negatively associated with NAT2
-
-
-
additional information
?
-
-
NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates
-
-
-
additional information
?
-
P50293
the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics
-
-
-
additional information
?
-
-
the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens
-
-
-
additional information
?
-
-
the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate
-
-
-
additional information
?
-
P18440
the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview
-
-
-
additional information
?
-
P50294, P50295
the isozymes catalyze the N-acetylation of aromatic amine and hydrazine drugs and carcinogens, after N-hydroxylation, they also catalyze the metabolic activation of N-hydroxy-arylamines via O-acetylation
-
-
-
additional information
?
-
-
variable expression of isozyme NAT1 due to genetic polymorphism, gene regulation or environmental influences is associated with individual susceptibility to various cancers, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acetyl-CoA
-
-
acetyl-CoA
A2V7M5
-
acetyl-CoA
P50294, P50295
;
acetyl-CoA
-
Glu60 and especially Lys136 play important roles in acetyl-CoA binding
acetyl-CoA
-
-
acetyl-CoA
Q6HVT9, Q81PT0, Q81R98
;
acetyl-CoA
-
-
acetyl-CoA
-
-
acetyl-CoA
Q5YYQ3
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
activation
K+
-
activation
Mg2+
-
activation
Na+
-
activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(-)-epigallocatechin-3-O-gallate
-
EGCG, non-competitive
(5Z)-3-amino-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(2-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
i.e. Rhod-o-hp. Significant reduction of cell growth by increasing the percent of MDA-MB-231 cells in G2/M phase of the cell cycle, and reduction of the ability of cells to grow in soft agar
(5Z)-5-(2-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(2-methylbenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3,4-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(4-chlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
1-butoxy-2-methylbenzene
-
-
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris, the component or extract has no effect on a Nat-deficient Mycobacterium bovis strain; a drimane sesquiterpenoid lactone isolated from Warburgia salutaris. Compound inhibits the growth of Mycobacterium bovis BCG wild-type in a dose-dependent fashion, while it had little effect of the growth of Mycobacterium bovis BCG nat deleted mutant
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris, 35% inhibition of the recombinant enzyme at 0.5 mg/ml; a novel drimane sesquiterpenoid lactone isolated from Warburgia salutaris. Compound inhibits recombinant NAT activity of Mycobacterium smegmatis (35% inhibition of acetylation at 0.5 mg/mL). Compound exhibits anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium bovis BCG Pasteur. Compound does not inhibit growth of Escherichia coli and is not toxic to cultured mammalian macrophage cells at the concentrations at which anti-mycobacterial activity is observed; isolated from the bark of Warburgia salutaris
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris
17-hydroxy-beta-estradiol
-
-
17-hydroxy-progesterone
-
-
2,3-Dihydroxybiphenyl
-
the growth of strain BCG is retarded when the cultures are incubated either in the presence of 2,3-dihydroxybiphenyl on both solid and liquid media
2-bromoacetanilide
-
irreversible inhibitor
2-butoxyphenol
-
-
2-nitrosofluorene
-
; potent inactivator, incubation with 2-nitrosofluorene causes 91% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 28%
2-nitrosotoluene
-
; less potent inactivator of NAT1, NAT1 with 2-nitrosotoluene causes 46% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 5%
3',5'-ADP
-
kinetics
3-chlorocatechol
-
the growth of strain BCG is retarded when the cultures are incubated either in the presence of 3-chlorocatechol on both solid and liquid media
4-hydroxy tamoxifen
-
-
4-nitrosobenzene
-
less potent inactivator of NAT1, NAT1 with nitrosobenzene causes 59% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 13%
4-nitrosobiphenyl
-
; potent inactivator, incubation with 4-nitrosobiphenyl causes 71% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 35%
5-aminosalicylic acid
-
substrate inhibition, in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex in non-productive reaction pathways
5-aminosalicylic acid
-
substrate inhibition; substrate inhibition through 5-aminosalicylic acid in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM in Colo 205 cells, inhibitory at lower dosage of 0.05-0.5 mM, concentrations of 5-25 mM have no effect in Colo 205 cells
acetoacetyl-CoA
-
0.1 mM, 44% inhibtion
acetoaminophen
-
-
Ag+
-
; 26% remaining activity isoenzyme Nat-b; 30% remaining activity isoenzyme NAT-a
alpha-zearalenol
-
-
AMP
-
kinetics
androstenedione
-
-
Berberine
-
berberine affects kinetic constants of NAT, 24 h berberine treatment decreases bacterial growth and amounts of 2-acetylaminofluorene in Salmonella typhimurium by downregulation of the NAT enzyme expression
beta-methylesculetin
-
inhibits NAT2 but not NAT1
beta-zearalenol
-
-
bisphenol A
-
-
bromoacetamide
-
NAT2
Bromoacetanilide
-
product analogue, kinetics
Butyryl-CoA
-
0.1 mM, 28% inhibtion
Ca2+
-
at increased concentrations
caffeic acid
-
4 mM, 65.3% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 37.9% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 37.6% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 39.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 83.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
non-competitive
caffeic acid
-
inhibits NAT1 but not NAT2
Chloro-beta-naphthalide
-
-
chlorogenic acid
-
dose-dependent enzyme inhibition in cytosolic and intact cell examinations
chlorogenic acid
-
4mM, significant inhibitory effect for 4 hours. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
chlorogenic acid
-
dose-dependent enzyme inhibition in cytosolic and intact cell examinations
cholate
-
-
cisplatin
-
; exposure of MCF-7 breast cancer cells to cisplatin at clinically relevant concentrations (below 0.4 nM) causes significant dose-dependent inhibition of the endogenous NAT1 enzyme. The incubation of NAT1 with various concentrations of cisplatin results in the dose-dependent modification of cysteine residues, as indicated by the disappearance of fluorescein-conjugated iodoacetamide labeling
cisplatin
-
treating C57BL/6J mice with cisplatin significantly decreases murine Nat2 (murine counterpart of human NAT1) enzymatic function in the liver (25% inhibition), kidney (40% inhibition), and blood cells (50% inhibition)
Co2+
-
; 39% remaining activity isoenzyme NAT-a
coenzyme A
-
-
coenzyme A
-
-
Cu2+
-
reversible by EDTA or histidine
Cu2+
-
; 30% remaining activity isoenzyme Nat-b
curcumin
-
non-competitive
curcumin
-
inhibits NAT2 but not NAT1
cytokine
-
mixture of proinflammatory cytokines, interferon-gamma, interleukin-1beta, tumor necrosis factor-alpha
-
deoxycholate
-
-
diethyldicarbonate
-
-
diethyldicarbonate
-
-
diethylstilbestrol
-
-
dihydrofolic acid
-
-
dithiothreitol
-
above 0.5 mM, not
dithiothreitol
-
-
DTNB
-
-
DTNB
-
partly reversible by dithioerythritol
EDTA
-
partly reversible by MgCl2
Ellagic acid
-
48.9% to 80.3% inhibition of the enzyme activity in the intact cell, time- and dose-dependent inhibition, ellagic acid suppresses enzyme activity in six clinically isolated Pseudomonas aeruginosa strains
Ellagic acid
-
-
esculetin
-
;
estrone
-
-
ferulic acid
-
4 mM, 46.5% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 41.4% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 35.8% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 39.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 75.1% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
-
ferulic acid
-
inhibits NAT1 but not NAT2
folic acid
-
competitive
gallic acid
-
-
gallic acid
-
inhibits NAT1 but not NAT2
genistein
-
;
genistein
-
-
glucosamine
-
-
glucosamine 6-phosphate
-
-
glutaryl CoA
-
0.1 mM, 34% inhibtion
glycyrrhizic acid
-
-
H2O2
-
NAT1 is reversibly inactivated by physiological aoncentrations of hydrogen peroxide. Inactivation of NAT1 is fully reversed by physiological concentrations of GSH
Hexanoyl-CoA
-
0.1 mM, 24% inhibtion
Hg2+
-
; 11% remaining activity isoenzyme Nat-b; 36% remaining activity isoenzyme NAT-a
hydrogen peroxide
-
inactivates isozyme NAT1, in vivo effect, overview
hydrogen peroxide
-
inhibition is caused by oxidation at the active site cysteine
Ibuprofen
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
Cys68 is the only site of alkylation, NAT2
iodoacetamide
-
50% inhibition at 0.25 mM, 90% inhibition at 1.0 mM
iodoacetamide
-
-
iodoacetamide
-
incubation of TBNAT with iodoacetamide results in a time-dependentloss of enzymatic activity
iodoacetic acid
-
-
iodoacetic acid
-
-
iodoacetic acid
-
-
iodoacetic acid
-
; 13% remaining activity
K+
-
at increased concentrations
kaemferol
-
non-competitive; non-competitive
kaempferol
-
inhibits NAT1 and NAT2
Ketoprofen
-
competitive inhibitor of NAT enzymes
luteolin
-
-
malonyl-CoA
-
0.1 mM, 11 inhibtion
methopterin
-
kinetics
methotrexate
-
inhibits reaction with 4-aminobenzoic acid, but with sulfamethazine, possibly due to two different enzymes
methotrexate
-
competitive
Mg2+
-
at increased concentrations
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-Hydroxy-2-acetylaminofluorene
-
mechanism-based inactivator, kinetics
N-Hydroxy-2-acetylaminofluorene
-
mechanism-based inactivator, kinetics
Na+
-
at increased concentrations
nitrosobenzene
-
-
Octanoyl-CoA
-
0.1 mM, 74% inhibtion
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-mercuribenzoate
-
-
p-mercuribenzoate
-
-
paclitaxel
-
inhibits NAT1 and NAT2
palmitoyl-CoA
-
-
peroxinitrite
-
rapid and irreversible inactivation
peroxynitrite
-
inactivates isozyme NAT1, in vivo effect, overview
peroxynitrite
-
inhibition is caused by oxidation at the active site cysteine
Phenylglyoxal
-
-
Phenylglyoxal
-
-
pregnenolone
-
-
proinflammatory cytokine
-
treatment of cholangiocarcinoma KKU-100 cells with cytokines (interferon-gamma, interleukin-1beta and tumor necrosis factor-alpha) suppresses NAT1 activity, reducing the Vmax without affecting the Km
-
propionyl-CoA
-
-
quercetin
-
non-competitive; non-competitive
quercetin
-
inhibits NAT1 and NAT2
S-nitroso-glutathione
-
treatment of cholangiocarcinoma KKU-100 cells S-nitroso-glutathione results in reduced NAT1 activity as early as 2 h, and the suppression persists for 48 h
S-nitroso-N-acetyl-DL-penicillamine
-
reversible inactivation due to direct atteck of the highly reactive cysteine residue in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between these NO-derived oxidants and NAT1
S-nitrosoglutathione
-
-
salicylic acid
-
-
scopoletin
-
-
silymarin
-
-
succinyl-CoA
-
0.1 mM, 96% inhibtion
tamoxifen
-
-
taurocholate
-
-
taxifolin
-
;
testosterone
-
-
Triton X-100
-
-
vitamin C
-
-
Warburgia salutaris extract
-
-
-
zearalenone
-
-
Zn2+
-
reversible by dimercaptosuccinate
Zn2+
-
; 39% remaining activity isoenzyme Nat-b; 41% remaining activity isoenzyme NAT-a
additional information
-
no inhibition by acetylated products and 2-mercaptoethanol
-
additional information
-
-
-
additional information
-
no inhibition by CdCl2, arsenite, diisopropylfluorophosphate
-
additional information
-
-
-
additional information
-
no inhibition by CdCl2, arsenite, diisopropylfluorophosphate; no inhibition by paraoxon
-
additional information
-
no significant inactivation with either iodoacetic acid or bromoacetic acid, NAT2
-
additional information
-
inhibitory potency of flavonoids on isozyme NAT1, no inhibition by curcumin, silymarin, and scopoletin, overview; inhibitory potency of flavonoids on isozyme NAT2, no inhibition by ()epigallocatechin gallate, gallic acid, caffeic acid, and ferulic acid, overview
-
additional information
-
inhibition profile by polyphenol compounds is different between NAT1 and NAT2. The small polyphenol of cinnamic acid derivates shows some inhibitory activity toward NAT1 , but it has very low activity toward NAT2
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
1 mM, activation
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM in Colo 205 cells, inhibitory at lower dosage of 0.05-0.5 mM in Colo 205 cells and at 0.5-0.25 mM in SC-M1 cells, concentrations of 5-25 mM have no effect in Colo 205 cells
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM and the further metabolism of the product of the enzyme catalytic reaction 2-acetylaminofluorene in the stomach and colon
Albumin
-
activation, with long-chain primary amines as substrate
-
cysteine
-
activation
Dimercaptosuccinate
-
1 mM, activation
Penicillamine
-
1 mM, activation
R1881
P18440
the androgen R1881 induces NAT1 activity in androgen receptor-positive prostate cancer cells
glycyrrhizic acid
-
slightly activates isozyme NAT1
additional information
-
no activation by EDTA or histidine
-
additional information
P18440
the androgen R1881 induces the enzyme about 4.5fold in androgen receptor AR-positive prostate cell lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells, androgen up-regulation of NAT1 is prevented by the AR antagonist flutamide, the effect of R1881 may not be by direct transcriptional activation of NAT1, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.11
2,3-dimethylaniline
-
-
5.53
2,3-dimethylaniline
-
-
2.06
2,4-dimethylaniline
-
-
5.03
2,4-dimethylaniline
-
-
3.12
2,5-dimethylaniline
-
-
6.73
2,5-dimethylaniline
-
-
0.81
2-amino-4-chlorophenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
1.9
2-amino-4-chlorophenol
-
-
1.9
2-amino-4-methylphenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
3.06
2-amino-4-methylphenol
-
-
13
2-aminobenzoic acid
-
-
0.000153
2-Aminofluorene
-
-
0.00019
2-Aminofluorene
-
-
0.00055
2-Aminofluorene
-
-
0.033
2-Aminofluorene
Q80SX3
recombinant isozyme Nat3
0.037
2-Aminofluorene
Q80SX3
recombinant isozyme Nat1
0.044
2-Aminofluorene
-
pH 7.4, 37C
0.064
2-Aminofluorene
-
pH 7.4, 37C
0.079
2-Aminofluorene
-
pH 7.4, 37C
0.082
2-Aminofluorene
-
pH 7.4, 37C
0.084
2-Aminofluorene
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
0.096
2-Aminofluorene
-
pH 7.4, 37C
0.106
2-Aminofluorene
-
pH 7.4, 37C
0.109
2-Aminofluorene
-
-
0.117
2-Aminofluorene
-
pH 7.4, 37C
0.127
2-Aminofluorene
-
strain 54.8, pH 7.4, 37C
0.141
2-Aminofluorene
-
pH 7.4, 37C
0.158
2-Aminofluorene
-
pH 7.4, 37C
0.165
2-Aminofluorene
-
pH 7.4, 37C
0.17
2-Aminofluorene
-
-
0.205
2-Aminofluorene
-
strain K-12, pH 7.4, 37C
0.21
2-Aminofluorene
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.238
2-Aminofluorene
-
wild-type enzyme
0.286
2-Aminofluorene
-
-
0.298
2-Aminofluorene
-
mutant enzyme I238T
0.301
2-Aminofluorene
-
mutant enzyme 245I
0.304
2-Aminofluorene
-
mutant enzyme W77R
0.311
2-Aminofluorene
-
strain 60.62, pH 7.4, 37C
0.365
2-Aminofluorene
-
strain YG1024, pH 7.4, 37C
0.367
2-Aminofluorene
-
mutant enzyme K185N
0.397
2-Aminofluorene
-
strain TA98, pH 7.4, 37C
0.404
2-Aminofluorene
-
strain TA1538, pH 7.4, 37C
0.504
2-Aminofluorene
-
-
0.568
2-Aminofluorene
Q80SX3
recombinant isozyme Nat2
0.72
2-Aminofluorene
-
-
0.81
2-Aminofluorene
-
-
0.842
2-Aminofluorene
-
-
0.857
2-Aminofluorene
-
mutant enzyme N172I
1.06
2-Aminofluorene
-
-
1.1
2-Aminofluorene
-
-
2.92
2-Aminofluorene
-
-
1.4
2-Aminophenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
5.79
2-Aminophenol
-
-
2.11
2-ethylaniline
-
-
2.32
2-methylaniline
-
-
7.18
2-methylaniline
-
-
1.1
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
1.2
2-toluidine
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
6.6
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.352
3,4-dimethylaniline
-
-
0.688
3,4-dimethylaniline
-
-
0.5
3,5-dimethyl-4-nitroaniline
-
-
0.28
3,5-dimethylaniline
-
-
0.742
3,5-dimethylaniline
-
-
2.6
3-amino-4-hydroxybenzaldehyde
A2V7M5
pH 7.0, 30C, recombinant enzyme
12
3-amino-4-hydroxybenzenesulfonic acid
A2V7M5
pH 7.0, 30C, recombinant enzyme
0.32
3-Amino-4-hydroxybenzoic acid
-
-
2
3-Amino-4-hydroxybenzoic acid
A2V7M5
pH 7.0, 30C, recombinant enzyme
12.5
3-aminobenzoic acid
-
-
0.576
3-ethylaniline
-
-
1.32
3-ethylaniline
-
-
0.9
4-amino-3-hydroxybenzoic acid
-
-
0.17
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
0.18
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
11
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.04
4-amino-4'-nitroazobenzene
-
-
2.42
4-Aminobenzoate
-
-
0.049
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
0.061
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.23
4-Aminobenzoic acid
P50292
pH 7.0, 25C, recombinant wild-type enzyme; pH 7.0, 25C, recombinant wild-type enzyme
0.37
4-Aminobenzoic acid
P50292
pH 7.0, 25C, recombinant mutant R64W; pH 7.0, 25C, recombinant mutant R64W
2.25
4-Aminobenzoic acid
-
-
2.34
4-Aminobenzoic acid
-
-
166
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.0058
4-aminobiphenyl
Q80SX3
recombinant isozyme Nat1
0.018
4-aminobiphenyl
Q80SX3
recombinant isozyme Nat3
0.191
4-aminobiphenyl
-
-
0.486
4-aminobiphenyl
-
-
1.57
4-aminobiphenyl
Q80SX3
recombinant isozyme Nat2
0.834
4-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.258
4-aminosalicylic acid
-
pH 7.4, 37C
0.86
4-aminosalicylic acid
-
pH 7.4, 37C
1.16
4-aminosalicylic acid
-
strain K-12, pH 7.4, 37C
1.24
4-aminosalicylic acid
-
strain 54.8, pH 7.4, 37C
1.79
4-aminosalicylic acid
-
pH 7.4, 37C
2.2
4-aminosalicylic acid
-
pH 7.4, 37C
1.8
4-anisidine
O86309
-
1.8
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
2.3
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
3.7
4-anisidine
-
wild-type, pH 8.0, 28C
1.8
4-bromoaniline
O86309
-
0.33
4-butoxyaniline
O86309
-
2
4-chloroaniline
O86309
-
6
4-chloroaniline
-
-
1.01
4-ethoxyaniline
O86309
-
0.205
4-ethylaniline
-
-
3.27
4-ethylaniline
-
-
51
4-fluoroaniline
-
-
20
4-hydroxybenzhydrazide
-
-
1
4-iodoaniline
O86309
-
0.483
4-methylaniline
-
-
11
4-methylaniline
-
-
118
4-methylaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.3
4-nitrobenzalhydrazone
-
-
2.8
4-nitrophenyl acetate
-
mutant Y190A, pH 7.0, 25C
3.5
4-nitrophenyl acetate
-
mutant Y190F, pH 7.0, 25C
4.1
4-nitrophenyl acetate
-
mutant Y190I, pH 7.0, 25C
4.81
4-nitrophenyl acetate
P50292
pH 7.0, 25C, recombinant mutant R64W; pH 7.0, 25C, recombinant mutant R64W
9
4-nitrophenyl acetate
-
wild-type, pH 7.0, 25C
10
4-nitrophenyl acetate
P50292
pH 7.0, 25C, recombinant wild-type enzyme; pH 7.0, 25C, recombinant wild-type enzyme
3.2
4-phenylenediamine
-
-
5.4
4-phenylenediamine
-
-
0.2
5-Aminosalicylate
-
-
0.228
5-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.25
5-Aminosalicylate
O86309
-
0.622
5-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
0.0067
5-aminosalicylic acid
P11245, P18440
isoform NAT 1, pH 7.4, 37C
0.026
5-aminosalicylic acid
-
pH 7.4, 37C
0.061
5-aminosalicylic acid
-
pH 7.4, 37C
0.112
5-aminosalicylic acid
-
pH 7.4, 37C
0.117
5-aminosalicylic acid
-
strain 54.8, pH 7.4, 37C
0.158
5-aminosalicylic acid
-
pH 7.4, 37C
0.184
5-aminosalicylic acid
P11245, P18440
isoform NAT 2, pH 7.4, 37C
0.208
5-aminosalicylic acid
-
strain 60.62, pH 7.4, 37C
0.21
5-aminosalicylic acid
-
pH 7.4, 37C
0.219
5-aminosalicylic acid
-
-
0.347
5-aminosalicylic acid
-
pH 7.4, 37C
1
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
1.02
5-aminosalicylic acid
-
-
1.2
5-aminosalicylic acid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
1.7
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
0.0084
acetyl-CoA
-
-
0.013
acetyl-CoA
-
-
0.02
acetyl-CoA
-
-
0.028
acetyl-CoA
-
-
0.05
acetyl-CoA
-
-
0.07
acetyl-CoA
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
0.11 - 0.125
acetyl-CoA
-
-
0.136
acetyl-CoA
-
concentration p-anisidine: 200 nM, reaction: acetyl-CoA + p-anisidine
0.158
acetyl-CoA
-
wild-type, pH 8.0, 28C
0.181
acetyl-CoA
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
0.2
acetyl-CoA
-
using 5-aminosalicylate as a substrate
0.466
acetyl-CoA
-
concentration p-anisidine: 2 mM, reaction: acetyl-CoA + p-anisidine
0.77
acetyl-CoA
-
-
1.02
acetyl-CoA
-
-
560
acetyl-CoA
-
wild-type, pH 8.0, 28C
2.49
aniline
-
-
112
aniline
-
-
14
benzoic acid hydrazide
-
-
0.77 - 0.8
decylamine
-
-
0.77 - 0.8
decylamine
-
-
0.3
dodecylamine
-
-
0.14
Hexylamine
-
-
0.72
histamine
-
-
0.08
hydralazine
O86309
-
0.61
hydralazine
-
-
0.849
hydralazine
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
1.327
hydralazine
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.71 - 0.72
hydroxytyramine
-
-
0.18
Isoniazid
-
-
0.366
Isoniazid
-
in vitro result for allele NAT2*7
0.374
Isoniazid
-
in vitro result for allele NAT2*4
1.414
Isoniazid
Q5YYQ3
; apparent KM
4.7
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
6.1
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
6.2
Isoniazid
-
wild-type, pH 8.0, 28C
0.38 - 0.58
isoniazide
-
-
3.2
N,N-1,4-phenylenediamine
-
Vmax: 0.17 micromol/min/mg
5.4
N,N-1,4-phenylenediamine
-
Vmax: 2.1 micromol/min/mg
0.058
N-Hydroxy-4-aminobiphenyl
Q80SX3
recombinant isozyme Nat1
0.062
N-Hydroxy-4-aminobiphenyl
Q80SX3
recombinant isozyme Nat3
3.3
N-Hydroxy-4-aminobiphenyl
Q80SX3
recombinant isozyme Nat2
14
nicotinic acid hydrazide
-
-
0.08
octylamine
-
-
0.08
octylamine
-
-
0.0028
p-Aminobenzoic acid
-
control; using control cholangiocarcinoma KKU-100 cells without cytokine administration, Vmax: 211.2 pmol/min/mg
0.0033
p-Aminobenzoic acid
-
cytokine mixture; using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount of enzyme but not in its affinity
0.01
p-Aminobenzoic acid
-
at fixed concentration of 0.1 mM acetyl-CoA
0.084
p-Aminobenzoic acid
-
-
0.283
p-Aminobenzoic acid
-
strain 60.62, pH 7.4, 37C
0.87
p-Aminobenzoic acid
-
pH 7.4, 37C
1.2
p-Aminobenzoic acid
-
pH 7.4, 37C
2.9
p-Aminobenzoic acid
-
at fixed concentration of 0.1 mM acetyl-CoA
5
p-Aminobenzoic acid
-
-
51.6
p-Aminobenzoic acid
-
strain YG1024, pH 7.4, 37C
152
p-Aminobenzoic acid
-
-
0.059
p-Aminosalicylic acid
-
-
0.184
p-Aminosalicylic acid
-
mutant G129A
0.216
p-Aminosalicylic acid
-
-
0.447
p-Aminosalicylic acid
-
mutant G129S
5.39
p-Aminosalicylic acid
-
-
0.136
p-anisidine
-
pH8, 37C
1.3
p-octopamine
-
liver
0.14 - 0.145
p-tyramine
-
-
0.14 - 0.145
p-tyramine
-
-
0.184
PAS
-
mutant G129A
0.216
PAS
-
wild-type
0.447
PAS
-
mutant G129S
0.18
Phenylethylamine
-
-
0.3
Phenylethylamine
-
-
45
procainamide
-
isozyme NAT1
0.11 - 0.125
serotonin
-
brain
0.25
serotonin
-
-
0.42
serotonin
-
-
3.1
Sulfadiazine
-
-
0.02
sulfamethazine
-
mutant F125S
0.059
sulfamethazine
-
strain 54.8, pH 7.4, 37C
0.07
sulfamethazine
-
pH 7.4, 37C
0.115
sulfamethazine
-
pH 7.4, 37C
0.12
sulfamethazine
-
at fixed concentration of 0.1 mM acetyl-CoA
0.121
sulfamethazine
-
strain K-12, pH 7.4, 37C
0.146
sulfamethazine
-
pH 7.4, 37C
0.55
sulfamethazine
-
pH 7.4, 37C
1.2
sulfamethazine
-
at fixed concentration of 0.1 mM acetyl-CoA
3.5
sulfamethazine
-
isozyme NAT1
5.39
sulfamethazine
-
-
0.662
sulfamethoxazole
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.07
tetradecylamine
-
-
0.031
tryptamine
-
-
0.24 - 0.25
tryptamine
-
-
0.24 - 0.25
tryptamine
-
liver
0.53
tryptamine
-
pineal gland
0.0088
tyramine
-
-
0.08
m-tyramine
-
-
additional information
additional information
-
reaction kinetics
-
additional information
additional information
-
kinetic data of various acetyl donors with 4-nitroaniline or aniline as acceptor amine and of various acceptor amines with acetyl-CoA as donor
-
additional information
additional information
-
assay based on HPLC
-
additional information
additional information
-
kinetic data of isozymes NAT1 and 2
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
not detectable: p-aminobenzoic acid
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
not detectable: 4-aminosalicylic-acid, pH 7.4, 37C
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
steady-state kinetics, kinetic and isotope effect studies
-
additional information
additional information
-
kinetics analysis, kinetic mechanism, determination of the half-life of the acetylated enzyme intermediate as 270 s, overview
-
additional information
additional information
-
kinetics; kinetics
-
additional information
additional information
-
NAT1 shows much higher affinity for p-amino benzoic acid than for sulfamethazine while NAT2 shows the opposite selectivity
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
27
2,3-dimethylaniline
-
-
256
2,3-dimethylaniline
-
-
67
2,4-dimethylaniline
-
-
661
2,4-dimethylaniline
-
-
5.7
2,5-dimethylaniline
-
-
313
2,5-dimethylaniline
-
-
0.27
2-amino-4-chlorophenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
21
2-amino-4-chlorophenol
-
-
1.3
2-amino-4-methylphenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
70
2-amino-4-methylphenol
-
-
449
2-Aminofluorene
-
-
759
2-Aminofluorene
-
-
0.41
2-Aminophenol
A2V7M5
pH 7.0, 30C, recombinant enzyme
51
2-Aminophenol
-
-
61
2-ethylaniline
-
-
12
2-methylaniline
-
-
111
2-methylaniline
-
-
4.8
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
6.3
2-toluidine
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
72
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
461
3,4-dimethylaniline
-
-
800
3,4-dimethylaniline
-
-
308
3,5-dimethylaniline
-
-
1220
3,5-dimethylaniline
-
-
0.29
3-amino-4-hydroxybenzaldehyde
A2V7M5
pH 7.0, 30C, recombinant enzyme
1.1
3-amino-4-hydroxybenzenesulfonic acid
A2V7M5
pH 7.0, 30C, recombinant enzyme
1.3
3-Amino-4-hydroxybenzoic acid
A2V7M5
pH 7.0, 30C, recombinant enzyme
94
3-Amino-4-hydroxybenzoic acid
-
-
310
3-ethylaniline
-
-
1960
3-ethylaniline
-
-
66
4-amino-3-hydroxybenzoic acid
-
-
1.3
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
14.2
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
16.6
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
8
4-Aminobenzoic acid
-
cosubstrate acetyl-CoA, mutant Y190A, pH 7.0, 25C
21
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
38
4-Aminobenzoic acid
-
cosubstrate 4-nitrophenyl acetate, mutant Y190A, pH 7.0, 25C
58
4-Aminobenzoic acid
-
cosubstrate acetyl-CoA, mutant Y190I, pH 7.0, 25C
60
4-Aminobenzoic acid
-
cosubstrate 4-nitrophenyl acetate, mutant Y190I, pH 7.0, 25C
127
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
189
4-Aminobenzoic acid
-
cosubstrate acetyl-CoA, mutant Y190F, pH 7.0, 25C
200
4-Aminobenzoic acid
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
200
4-Aminobenzoic acid
-
cosubstrate acetyl-CoA, wild-type, pH 7.0, 25C
230
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
265
4-Aminobenzoic acid
P50292
pH 7.0, 25C, recombinant mutant R64W, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant mutant R64W, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant wild-type enzyme, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant wild-type enzyme, with 4-nitrophenyl acetate
393
4-Aminobenzoic acid
-
cosubstrate 4-nitrophenyl acetate, mutant Y190F, pH 7.0, 25C
620
4-Aminobenzoic acid
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
620
4-Aminobenzoic acid
-
cosubstrate 4-nitrophenyl acetate, wild-type, pH 7.0, 25C
16
4-aminobenzoyl glutamic acid
-
mutant Y190A, pH 7.0, 25C
76
4-aminobenzoyl glutamic acid
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
77
4-aminobenzoyl glutamic acid
-
mutant Y190I, pH 7.0, 25C
86
4-aminobenzoyl glutamic acid
-
mutant Y190F, pH 7.0, 25C
120
4-aminobenzoyl glutamic acid
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
120
4-aminobenzoyl glutamic acid
-
wild-type, pH 7.0, 25C
243
4-aminobiphenyl
-
-
256
4-aminobiphenyl
-
-
0.03
4-anisidine
O86309
-
0.3
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
0.6
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5.2
4-anisidine
-
wild-type, pH 8.0, 28C
0.0086
4-bromoaniline
O86309
-
0.039
4-butoxyaniline
O86309
-
0.0069
4-chloroaniline
O86309
-
0.2
4-chloroaniline
-
-
0.036
4-ethoxyaniline
O86309
-
25
4-ethylaniline
-
-
430
4-ethylaniline
-
-
700
4-ethylaniline
-
-
0.9
4-fluoroaniline
-
-
10.9
4-hydroxybenzhydrazide
-
-
0.0098
4-iodoaniline
O86309
-
0.8
4-methylaniline
-
-
303
4-methylaniline
-
-
1600
4-methylaniline
-
-
0.25
4-nitroaniline
-
mutant Y190A, pH 7.0, 25C
0.31
4-nitroaniline
-
mutant Y190F, pH 7.0, 25C
0.6
4-nitroaniline
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
0.6
4-nitroaniline
-
wild-type, pH 7.0, 25C
0.89
4-nitroaniline
-
mutant Y190I, pH 7.0, 25C
15
4-nitrophenyl acetate
-
mutant Y190A, pH 7.0, 25C
57
4-nitrophenyl acetate
-
mutant Y190I, pH 7.0, 25C
279
4-nitrophenyl acetate
-
mutant Y190F, pH 7.0, 25C
620
4-nitrophenyl acetate
P50292
pH 7.0, 25C, recombinant mutant R64W, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant mutant R64W, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant wild-type enzyme, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant wild-type enzyme, with 4-aminobenzoic acid
1301
4-nitrophenyl acetate
-
wild-type, pH 7.0, 25C
0.044
5-Aminosalicylate
O86309
-
0.0017
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
1.8
5-aminosalicylic acid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5.2
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
434
5-aminosalicylic acid
-
-
281
aniline
-
-
711
aniline
-
-
2 - 8
anisidine
-
mutant Y190A, pH 7.0, 25C
85
anisidine
-
mutant Y190I, pH 7.0, 25C
260
anisidine
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
260
anisidine
-
wild-type, pH 7.0, 25C
288
anisidine
-
mutant Y190F, pH 7.0, 25C
1.9
benzoic acid hydrazide
-
-
0.063
hydralazine
O86309
-
44
hydralazine
-
-
0.1
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
0.3
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
1.7
Isoniazid
-
wild-type, pH 8.0, 28C
0.6
nicotinic acid hydrazide
-
-
0.7
p-Aminobenzoic acid
-
-
38
p-Aminobenzoic acid
-
-
298
p-Aminobenzoic acid
-
-
27
p-Aminosalicylic acid
-
-
591
p-Aminosalicylic acid
-
-
387
sulfamethazine
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.166
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
3894
0.26
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
3894
0.018
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
1817
1.5
5-aminosalicylic acid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
1817
3.058
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
1817
0.166
anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
5655
0.26
anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5655
1.405
anisidine
-
wild-type, pH 8.0, 28C
5655
0.009
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
851
0.121
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
851
0.274
Isoniazid
-
wild-type, pH 8.0, 28C
851
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.54
4-nitrosobenzene
-
-
0.00067
4-nitrosobiphenyl
-
; recombinant NAT1
0.54
nitrosobenzene
-
recombinant NAT1
0.01
quercetin
-
isozyme NAT2
0.0486
quercetin
-
isozyme NAT1
0.67
Bromoacetanilide
-
-
additional information
additional information
-
inactivation kinetics of NAT1 by oxidants, overview
-
additional information
additional information
-
inhibition kinetics, isozyme NAT1; inhibition kinetics, isozyme NAT2
-
additional information
additional information
-
the second-order rate constant (kinact) for the inactivation of NAT1 by cisplatin is estimated at 700/M min
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0011
(5Z)-3-amino-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0011
(5Z)-5-(2-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0039
(5Z)-5-(2-methylbenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0034
(5Z)-5-(3,4-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0018
(5Z)-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0006
(5Z)-5-(3-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.0047
(5Z)-5-(4-chlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
pH 8.0, 25C
0.055
17-hydroxy-beta-estradiol
-
-
0.5
17-hydroxy-progesterone
-
value above 0.5 mM
0.00004
2-nitrosofluorene
-
; HeLa cell NAT1
0.091
2-nitrosotoluene
-
; HeLa cell NAT1
0.237
4-nitrosobenzene
-
-
0.00006
4-nitrosobiphenyl
-
; HeLa cell NAT1
0.065
4-OH tamoxifen
-
-
0.07
alpha-zearalenol
-
-
0.5
androstenedione
-
value above 0.5 mM
0.45
beta-zearalenol
-
-
0.29
bisphenol A
-
-
0.1
cisplatin
-
in MCF-7 breast cancer cells; in MCF7-cells, similar results with MDA-MB-231 cells
0.05
diethylstilbestrol
-
-
0.37
estrone
-
-
0.045
genistein
-
-
0.237
nitrosobenzene
-
HeLa cell NAT1
0.1
pregnenolone
-
value above 0.1 mM
0.057
tamoxifen
-
-
0.5
testosterone
-
value above 0.5 mM
0.22
zearalenone
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000000001
-
strain K-12, sulfamethazine, pH 7.4, 37C
0.000000001
-
sulfamethazine, pH 7.4, 37C
0.000000001
-
2-aminofluorene, pH 7.4, 37C
0.000000002
-
p-aminobenzoic acid, pH 7.4, 37C
0.000000002
-
sulfamethazine, pH 7.4, 37C
0.000000002
P11245, P18440
isoform NAT 2, p-aminobenzoic acid, pH 7.4, 37C
0.000000002
-
2-aminofluorene, pH 7.4, 37C
0.000000002
-
sulfamethazine, pH 7.4, 37C
0.000000003
-
sulfamethazine, pH 7.4, 37C
0.000000004
-
procainamide, pH 7.4, 37C
0.000000004
-
strain 54.8, 4-aminosalicylic acid, pH 7.4, 37C
0.000000004
-
2-aminofluorene, pH 7.4, 37C
0.000000005
-
p-aminobenzoic acid, pH 7.4, 37C
0.000000005
-
4-aminosalicylic acid, pH 7.4, 37C
0.000000005
-
strain 60.62, p-aminobenzoic acid, pH 7.4, 37C
0.000000005
-
4-aminosalicylic acid, pH 7.4, 37C
0.000000006
-
4-aminosalicylic acid, pH 7.4, 37C
0.000000006
-
procainamide, pH 7.4, 37C
0.000000006
-
5-aminosalicylic acid, pH 7.4, 37C
0.000000007
-
2-aminofluorene, pH 7.4, 37C
0.000000008
-
4-aminosalicylic acid, pH 7.4, 37C
0.00000001
-
strain K-12, 4-aminosalicylic acid, pH 7.4, 37C
0.00000001
-
2-aminofluorene, pH 7.4, 37C
0.000000014
-
strain YG1024, p-aminobenzoic acid, pH 7.4, 37C
0.00000002
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000002
-
strain K-12, 2-aminofluorene, pH 7.4, 37C
0.00000002
-
4-aminosalicylic acid, pH 7.4, 37C
0.00000004
-
strain TA1538, 2-aminofluorene, pH 7.4, 37C
0.00000005
-
p-aminobenzoic acid, pH 7.4, 37C
0.00000005
-
strain 54.8, 2-aminofluorene, pH 7.4, 37C
0.00000006
P11245, P18440
isoform NAT 2, procainamide, pH 7.4, 37C
0.00000006
-
strain 60.62, 2-aminofluorene, pH 7.4, 37C; strain TA98, 2-aminofluorene, pH 7.4, 37C
0.000000075
P11245, P18440
isoform NAT 2, 4-aminosalicylic acid, pH 7.4, 37C
0.00000009
-
2-aminofluorene, pH 7.4, 37C
0.00000009
-
strain 54.8, 5-aminosalicylic acid, pH 7.4, 37C
0.0000001
-
5-aminosalicylic acid, pH 7.4, 37C
0.0000001
-
2-aminofluorene, pH 7.4, 37C
0.00000014
-
2-aminofluorene, pH 7.4, 37C
0.00000014
-
p-aminobenzoic acid, pH 7.4, 37C
0.00000015
-
2-aminofluorene, pH 7.4, 37C
0.00000016
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000016
-
2-aminofluorene, pH 7.4, 37C
0.00000027
-
4-aminosalicylic acid, pH 7.4, 37C
0.00000027
-
strain 60.62, 5-aminosalicylic acid, pH 7.4, 37C
0.00000032
-
2-aminofluorene, pH 7.4, 37C
0.00000033
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000059
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000061
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000099
P11245, P18440
isoform NAT 2, 2-aminofluorene, pH 7.4, 37C
0.00000106
-
2-aminofluorene, pH 7.4, 37C
0.00000189
P11245, P18440
isoform NAT 2, 5-aminosalicylic acid, pH 7.4, 37C
0.0000022
-
5-aminosalicylic acid, pH 7.4, 37C
0.00000241
P11245, P18440
isoform NAT 2, sulfamethazine, pH 7.4, 37C
0.00000568
P11245, P18440
isoform NAT 1, procainamide, pH 7.4, 37C
0.0000083
P11245, P18440
isoform NAT 1, sulfamethazine, pH 7.4, 37C
0.0000208
-
strain YG1024, 5-aminosalicylic acid, pH 7.4, 37C
0.0000275
-
strain YG1024, 2-aminofluorene, pH 7.4, 37C
0.0001
-
breast cancer cell line Cal51c, estrogen receptor (ER)-negative; CAL-51 cell
0.000162
-
cytosol
0.0002
-
breast cancer cell line MDA-MB-231, estrogen receptor (ER)-negative; MDA-MB-231 cell
0.0005
-
mutant GGSG; mutant HHEH
0.00052
P11245, P18440
isoform NAT 1, 2-aminofluorene, pH 7.4, 37C
0.00058
P11245, P18440
isoform NAT 1, p-aminobenzoic acid, pH 7.4, 37C
0.000592
-
cytosol, 37C
0.0006
-
mutant GGSG; mutant HHEH
0.00068
-
cytosol
0.0007
-
breast cancer cell line MDA-MB-436, estrogen receptor (ER)-negative; MDA-MB-436 cell
0.001
-
breast cancer cell line MCF-7, estrogen receptor (ER)-positive
0.00109
P11245, P18440
isoform NAT 1, 4-aminosalicylic acid, pH 7.4, 37C
0.00117
P11245, P18440
isoform NAT 1, 5-aminosalicylic acid, pH 7.4, 37C
0.0013
-
mutant GRSG
0.0014
-
mutant GRSG
0.00161
-
HeLa cell + N-acetyl-L-cysteine + 4-nitrosobiphenyl
0.00172
-
HeLa cell + 4-nitrosobiphenyl
0.0018
-
MCF-7 cell
0.0021
-
breast cancer cell line MDA-MB-453, estrogen receptor (ER)-negative; MDA-MB-453 cell
0.00408
-
in HeLa cells, substrate: p-aminosalicylic acid
0.0044
-
in HeLa cells, substrate: p-aminobenzoic acid
0.00454
-
HeLa cell + N-acetyl-L-cysteine
0.00466
-
HeLa cell
0.00478
-
cytosol
0.005
-
breast cancer cell line T47D, estrogen receptor (ER)-positive
0.0054
-
T-47D cell
0.0055
-
mutant GG
0.006
-
mutant GG
0.0061
-
-
0.0064
-
-
0.008
-
enzymatic activity found in peripheral blood mononuclear cell of investigated donors: 8-23.5 nmol/mg/min
0.01
P18440
HEK-293 cell, treated with R1881; PC-3 cell
0.0104
P18440
effect of androgen receptor expression in PC-3 cells, control, plus R1881
0.0105
-
enzymatic activity found in HepG2 cells
0.0109
P18440
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1, plus R1881
0.011
P18440
HEK-293 cell; PC-3 cell, treated with R1881
0.0113
P18440
effect of androgen receptor expression in PC-3 cells, control
0.0115
P18440
effect of androgen receptor expression in PC-3 cells, plus pCMV-AR3.1
0.013
Q5YYQ3
substrate: 2-aminofluorene
0.014
P18440
HeLa cell
0.015
P18440
HeLa cell, treated with R1881
0.015
Q5YYQ3
substrate: sulfamethazine; sulfamethazine
0.022
-
mutant G129V
0.023
-
mutant G129V
0.023
Q5YYQ3
substrate: beta-naphthylamine
0.0234
-
enzymatic activity found in monocyte-derived dendritic cells of investigated donors: 23.4-26.6 nmol/mg/min
0.024
Q5YYQ3
substrate: 4-aminobiphenyl
0.02463
Q5YYQ3
substrate: 5-aminosalicylate
0.029
Q5YYQ3
substrate: sulfamethoxazole; sulfamethoxazole
0.034
-
mutant G129T
0.038
P18440
22Rv1 cell, plus R1881, plus flutamide
0.04
P18440
22Rv1 cell, 24h; 22Rv1 cell, 48h; 22Rv1 cell, plus bicalutamide, 24h; 22Rv1 cell, plus bicalutamide, 48h; 22Rv1 cell, plus R1881, plus bicalutamide; 22Rv1 cell, plus R1881, plus bicalutamide, 24h
0.042
P18440
22Rv1 cell, plus DMSO
0.045
P18440
LNCaP cell
0.046
P18440
22Rv1 cells and LNCAP cells (both androgene-positive lines) show a high basal level of NAT1 activity between 46-51 nmol/min/mg protein
0.05
P18440
22Rv1 cell
0.052
Q5YYQ3
substrate: benzidine
0.054
-
cytokine mixture, 2.5 microM p-aminobenzoic acid
0.07
P18440
22Rv1 cell, plus R1881; LNCaP cell, treated with R1881
0.079
Q5YYQ3
substrate: 4-aminoveratrole
0.08
P18440
22Rv1 cell, plus R1881, 24h
0.087
Q5YYQ3
substrate: hydralazine
0.097
-
cytokine mixture, 7.5 microM p-aminobenzoic acid
0.108
-
control, 2.5 microM p-aminobenzoic acid
0.111
Q5YYQ3
4-aminobenzoic acid; substrate: 4-aminobenzoic acid
0.124
-
cytokine mixture, 40 microM p-aminobenzoic acid
0.13
P18440
22Rv1 cell, treated with R1881
0.14
P18440
22Rv1 prostate cells upon treatment with 100 nmol of synthetic androgen R1881 for 24h
0.145
-
2-aminofluorene
0.146
-
control, 7.5 microM p-aminobenzoic acid
0.168
Q6HVT9, Q81PT0, Q81R98
; enzymatic activity detected in the absence of an arylamine substrate: BanatB, but not BanatC, displays AcCoA and /or p-nitrophenyl acetate hydrolysis activity in the absence of an arylamine substrate
0.177
-
sulfamethazine
0.19
-
after streptomycin sulfate treatment
0.2
-
control, 40 microM p-aminobenzoic acid
0.202
-
breast cancer cell line ZR-75-1, estrogen receptor (ER)-positive
0.2022
-
ZR-75-1 cell
0.237
-
recombinant NAT1, substrate: p-aminobenzoic acid
0.25
-
cell extract
0.29
A2V7M5
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzenesulfonic acid
0.33
A2V7M5
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzaldehyde
0.337
-
recombinant NAT1, substrate: p-aminosalicylic acid
0.372
Q5YYQ3
4-aminosalicylate; substrate: 4-aminosalicylate
0.38
-
purification step ammonium sulfate precipitation
0.41
-
2-aminofluorene
0.416
Q6HVT9, Q81PT0, Q81R98
substrate: sulfamethoxazole, isoenzyme BanatC; sulfamethoxazole
0.46
-
4-aminobenzoic acid
0.46
A2V7M5
purified recombinant enzyme, with substrate 2-amino-4-chlorophenol
0.47
A2V7M5
purified recombinant enzyme, with substrate 2-aminophenol
0.532
-
4-aminobenzoic acid L-glutamate, pH 8, 37C
0.55
-
-
0.567
-
substrate aniline, p-nitrophenylacetate as an acetyl donor; substrates: aniline and p-nitrophenyl acetate
0.602
-
substrate isoniazid, p-nitrophenylacetate as an acetyl donor; substrates: isoniazid and p-nitrophenyl acetate
0.603
Q6HVT9, Q81PT0, Q81R98
2-aminofluorene; substrate: 2-aminofluorenea
0.629
-
aniline, pH 8, 37C
0.629
-
substrate aniline, AcCoA as an acetyl donor; substrates: aniline and acetyl-CoA
0.641
-
4-methoxyphenylhydrazine, pH 8, 37C
0.67
-
2-aminofluorene
0.75
-
4-aminobenzoic acid
0.841
-
substrate p-aminobenzoic acid, p-nitrophenylacetate as an acetyl donor; substrates: p-aminobenzoic acid and p-nitrophenyl acetate
0.91
-
2-aminofluorene
0.969
Q6HVT9, Q81PT0, Q81R98
hydralazine; substrate: hydralazine
1
-
sulphisoxazole, pH 8, 37C
1.04
-
substrate 5-aminosalicylic acid, p-nitrophenylacetate as an acetyl donor; substrates: 5-aminosalicylic acid and p-nitrophenyl acetate
1.1
A2V7M5
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzoic acid or 2-amino-4-methylphenol
1.31
-
sulphametoxazole, pH 8, 37C
1.36
Q6HVT9, Q81PT0, Q81R98
5-aminosalicylate; substrate: 5-aminosalicylate
1.47
-
substrate 2-aminofluorene, p-nitrophenylacetate as an acetyl donor; substrates: 2-aminofluorene and p-nitrophenyl acetate
1.744
Q6HVT9, Q81PT0, Q81R98
hydralazine; substrate: hydralazine, isoenzyme BanatC
2.22
-
substrate p-anisidine, p-nitrophenylacetate as an acetyl donor; substrates: p-anisidine and p-nitrophenyl acetate
2.324
-
isoniazid, pH 8, 37C
2.324
-
substrate isoniazid, AcCoA as an acetyl donor; substrates: isoniazid and acetyl-CoA
2.54
-
4-aminobenzoic acid
2.76
-
4-trifluoromethylaniline, pH 8, 37C
2.878
Q5YYQ3
isoniazid; substrate: isoniazid
2.89
-
2-aminofluorene
2.99
-
substrate hydralazine, p-nitrophenylacetate as an acetyl donor; substrates: hydralazine and p-nitrophenyl acetate
3.109
Q6HVT9, Q81PT0, Q81R98
4-aminosalicylate; substrate: 4-aminosalicylate, isoenzyme BanatC
3.679
Q6HVT9, Q81PT0, Q81R98
5-aminosalicylate; substrate: 5-aminosalicylate, isoenzyme BanatC
4.14
-
4-chloroaniline, pH 8, 37C
4.152
-
mutant G129S
4.758
-
mutant G129A
5.078
-
wild-type; wild-type enzyme
5.71
-
4-bromoaniline, pH 8, 37C
5.852
Q6HVT9, Q81PT0, Q81R98
2-aminofluorene; substrate: 2-aminofluorene, isoenzyme BanatC
6.76
-
3,4-dichloroaniline, pH 8, 37C
7
-
purification step POROS 20PE column
7.3
-
purification step DE52-cellulose column
8.2
-
4-aminobenzoic acid, pH 8, 37C
8.2
-
substrate p-aminobenzoic acid, AcCoA as an acetyl donor; substrates: p-aminobenzoic acid and acetyl-CoA
9
-
purification step DE52-cellulose column; purification step POROS 20PE column
12.05
-
4-hexyloxyaniline, pH 8, 37C
13.03
Q5YYQ3
4-aminofluorene
13.47
-
4-anisidine, pH 8, 37C
13.5
-
substrate p-anisidine, AcCoA as an acetyl donor; substrates: p-anisidine and acetyl-CoA
13.8
-
4-iodoaniline, pH 8, 37C
14
-
4-chorobenzoic hydrazide, pH 8, 37C
14.02
-
-
18.66
-
4-aminosalicylate, pH 8, 37C
19.24
-
4-ethoxyaniline, pH 8, 37C
21.97
-
4-phenoxyaniline, pH 8, 37C
23.73
Q5YYQ3
beta-naphthylamine
24.63
Q5YYQ3
5-aminosalicylate
24.91
Q5YYQ3
2-aminobiphenyl
25.66
-
4-butoxyaniline, pH 8, 37C
26
-
purification step DEAE Toyopearl column
27
-
purification step DEAE Toyopearl column
28
-
thrombin cleaved fusion protein
29.55
-
hydralazine, pH 8, 37C
29.55
-
substrate hydralazine, AcCoA as an acetyl donor; substrates: hydralazine and acetyl-CoA
44.51
-
4-aminoveratrole, pH 8, 37C
44.71
-
2-aminofluorene, pH 8, 37C
44.71
-
substrate 2-aminofluorene, AcCoA as an acetyl donor; substrates: 2-aminofluorene and acetyl-CoA
52.33
Q5YYQ3
benzidine
73.3
-
5-aminosalicylate, pH 8, 37C
73.3
-
substrate 5-aminosalicylic acid, AcCoA as an acetyl donor; substrates: 5-aminosalicylic acid and acetyl-CoA
79.39
Q5YYQ3
4-aminoveratrole
87.76
Q5YYQ3
hydralazine
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
no detectable activity: sulphacetamide, sulphanilamide, sulphadiazine, sulphamethazine, trimethoprim, phenylhydrazine
additional information
-
Vmax 0.0034
additional information
-
Vmax 0.0293
additional information
-
intact cell: 1.37 nmol/min/10 000 000 000 CFU; Vmax 2-aminofluorene: 0.01715, in the presence of ellagic acid: 0.001732
additional information
-
activity below 0.000000001 2-aminofluorene, 4-aminosalicylic acid, 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 2-aminofluorene, 4-aminosalicylic acid, 5-aminosalicylic acid, procainamide, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 2-aminofluorene, 4-aminosalicylic acid, procainamide, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 4-aminosalicylic acid, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 procainamide, p-aminobenzoic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 p-aminobenzoic acid, procainamide, activity below 0.000000002 sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 procainamide, p-aminobenzoic acid, pH 7.4, 37C
additional information
-
below 0.000000001 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, strain K-12, pH 7.4, 37C; below 0.000000003 sulfamethazine, below 0.000000001 procainamide, p-aminobenzoic acid, strain 54.8 pH 7.4, 37C
additional information
-
activity below 0.000000002 5-aminosalicylic acid, activity below 0.000000001 4-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
P11245, P18440
Vmax 0.00000298, isoform NAT 2, 5-aminosalicylic acid, pH 7.4, 37C; Vmax 0.001715, isoform NAT 1, 5-aminosalicylic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 4-aminosalicylic acid, procainamide, p-aminobenzoic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 5-aminosalicylic acid, 4-aminosalicylic acid, p-aminobenzoic acid, procainamide, pH 7.4, 37C
additional information
-
not detectable 5-aminosalicylic acid, 4-aminosalicylic acid, p-aminobenzoic acid, procainamide, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000002 4-aminosalicylic acid, p-aminobenzoic acid, procainamide, sulfamethazine, not detectable 5-aminosalicylic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 2-aminofluorene, not detectable 4-aminosalicylic acid, 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000002 5-aminosalicylic acid, activity below 0.000000001 2-aminofluorene, 4-aminosalicylic acid, sulfamethazine, p-aminobenzoic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 2-aminofluorene, 4-aminosalicylic acid, 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 procainamide, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 strain 60.62, 4-aminosalicylic acid, sulfamethazine, pH 7.4, 37C; activity below 0.000000001 strain Ta1538, 4-aminosalicylic acid, p-aminobenzoic acid, sulfamethazine, procainamide, pH 7.4, 37C, not detectable: 5-aminosalicylic acid; activity below 0.000000001 strain Ta1538, 4-aminosalicylic acid, sulfamethazine, procainamide, below 0.000000002 p-aminobenzoic acid, pH 7.4, 37C, not detectable: 5-aminosalicylic acid; activity below 0.000000001 strain TA98 1,8/DNP, 2-aminofluorene, 5-aminosalicylic acid, 4-aminosalicylic acid, p-aminobenzoic acid, sulfamethazine, procainamide, pH 7.4, 37C; activity below 0.000000001 strain YG1024, 4-aminosalicylic acid, procainamide, sulfamethazine, pH 7.4, 37C
additional information
-
activity below 0.000000001 4-aminosalicylic acid, procainamide, p-aminobenzoic acid, pH 7.4, 37C
additional information
-
activity below 0.000000001 2-aminofluorene, 5-aminosalicylic acid, 4-aminosalicylic acid, p-aminobenzoic acid, activity below 0.000000006 procainamide, pH 7.4, 37C
additional information
-
activity below 0.000000001 5-aminosalicylic acid, procainamide, p-aminobenzoic acid, sulfamethazine, pH 7.4, 37C
additional information
-
-
additional information
-
-
additional information
P18440
it is shown that NAT1 activity is induced by synthetic androgen R1881 in androgen receptor (AR)-positive prostate lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells. The effect of R1881 is dose dependent, with an EC50 for R1881 of 1.6 nmol/L
additional information
A2V7M5
-
additional information
P50292
;
additional information
-
;
additional information
-
-
additional information
Q80SX3
substrate specificities of isozymes Nat1-Nat3
additional information
-
activity of recombinant wild-type and mutant NAT2, overview
additional information
-
isozyme activities in healthy and tumor breast tissue, overview; isozyme activities in healthy and tumor breast tissue, overview
additional information
-
enzymatic activity of mutants G129I and G129P are very low and not accurately measurable; mutant G129I and G129P display only very low enzymatic activity which can not be measured accurately
additional information
Q6HVT9, Q81PT0, Q81R98
BanatA is devoid of NAT or AcCoA/p-nitrophenyl acetate hydrolysis activities, suggesting that it may be a new bacterial NAT-like protein with unknown function; no activity with substrates: isoniazid, 4-aminobenzoic acid, isoenzyme BanatC; substrate: sulfamethoxazolea, 4-aminobenzoic, 4-aminosalicylate, isoniazid acid no activity detected
additional information
-
NAT1 activity is highly regulated by oxidative stress. NAT1 activity is highly sensitive to reactive oxygen or nitrogen species an is reversibly inactivated by H2O2
additional information
-
low enzymatic activity as a recombinant protein
additional information
-
relative activity isoenzyme NAT-a (substrate): 12% (aniline), 90% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 72% (2-aminophenol), 33% (3-aminophenol), 94% (4-aminophenol), 0% (2-aminobenzoic acid), 122% (3-aminobenzoic acid), 19% (4-aminobenzoic acid), 0% (2-nitroaniline), 2% (3-nitroaniline), 0% (4-nitroaniline), 18% (2-chloraniline), 48% (3-chloraniline), 18% (4-chloraniline), 0% (2-toluidine), 21% (3-toluidine), 80% (4-toluidine), 46% (sulfamethazine), 90% (4-aminosalicylic acid), 550% (5-aminosalicylic acid), 0% (4-aminoacetanilide); relative activity isoenzyme Nat-b (substrate): 24% (aniline), 80% (2-phenylendiamine), 0% (3-phenylendiamine), 100% (4-phenylendiamine), 36% (2-aminophenol), 56% (3-aminophenol), 48% (4-aminophenol), 0% (2-aminobenzoic acid), 105% (3-aminobenzoic acid), 26% (4-aminobenzoic acid), 3% (2-nitroaniline), 6% (3-nitroaniline), 0% (4-nitroaniline), 12% (2-chloraniline), 48% (3-chloraniline), 21% (4-chloraniline), 0% (2-toluidine), 28% (3-toluidine), 40% (4-toluidine), 0% (sulfamethazine), 100% (4-aminosalicylic acid), 510% (5-aminosalicylic acid), 0% (4-aminoacetanilide)
additional information
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MMNAT is found to acetylate a broad range of substrates, including isoniazid, 4-anisidine and 4-aminoveratrole, compounds that have been identified as substrates of the NAT enzyme of Mycobacterium tuberculosis. The specific activity profile differs somewhat from that of the other characterised mycobacterial NAT, NAT from Mycobacterium smegmatis, particularly in the rate of acetylation of hydralazine and 2-aminofluorene, which are both acetylated 100times more rapidly by MMNAT
additional information
-
wild-type enzyme 100% activity, + cisplatin 9%, + AcCoA and cisplatin 13 - 77%, depending on the AcCoA concentration, + CoA and cysplatin 7%
additional information
Q7YRG5
specific activities of various substrates (pmol/min/luminescence unit): 183 (p-anisidine), 4 (procainamide), 3 (sulphamethazine), 11 (5-aminosalicylate), 3 (p-aminobenzoic acid); specific activities of various substrates (pmol/min/luminescence unit): 197 (p-anisidine), 18 (procainamide), 12 (sulphamethazine), 2 (5-aminosalicylate), 0 (p-aminobenzoic acid)
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
isoniazide, sulfanilamide
5.6
-
sulfamethazine, 2 optima
5.7 - 7.4
-
sulfamethazine
5.7
-
sulfadiazine
5.9
-
4-nitroaniline
6.4
-
1,2-phenylenediamine
7
-
1,3-phenylenediamine
7
-
activity with 2-aminofluorene and 4-aminobenzoate
7
A2V7M5
assay at
7
P50292
assay at; assay at
7
-
assay at; assay at
7
-
assay at
7.1
-
activity assay; assay at
7.2
-
sulfamethazine, 2 optima
7.3
-
tricine buffer
7.4
P18440
activity assay; assay at; assay at
7.4
P50294, P50295
assay at; assay at
7.4
-
assay at
7.4
Q7YRG5
assay at
7.5 - 8.5
-
-
7.5
-
potassium phosphate or piperazine-N,N-bis(2-ethanolsulfonic acid) buffer
7.5
-
-
7.5
-
assay at
7.5
-
assay at; assay at
7.5
Q6HVT9, Q81PT0, Q81R98
activity assay; activity assay; activity assay; assay at; assay at; assay at
7.5
-
assay at; in vitro N-acetylation assay
7.5
-
enzyme assayed at various pHs at 30C; pH-optimum
7.5
Q5YYQ3
activity assay; assay at
7.5
-
assay at
7.5
-
activity assay; assay at
7.7 - 8.6
-
-
7.7
-
activity assay; assay at
7.8
-
tyramine in phosphate buffer
7.9
-
1,4-phenylenediamine
8
-
assay at
8
-
activity assay; assay at
8
-
enzyme assayed at various pHs at 30C; pH-optimum
8
-
activity assay; assay at
8.3
-
N-tris(hydroxymethyl)methyl-2-aminoethane buffer
9.1
-
benzylamine
9.5
-
cyclohexylamineopropanesulfonic acid buffer
9.5
-
0.1 M glycine/KOH buffer
9.8
-
trisethanolamine buffer
additional information
-
pH-optimum depends on buffer
additional information
-
pI: 4.8
additional information
-
pH-optimum depends on the nature of the acetyl acceptor amine
additional information
-
pI: 4.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9.5
-
about 25% of maximal activity pH 5.0 and pH 9.5 with 2-aminofluorene as substrate, about 65% of maximal activity at pH 5.0 and at pH 9.5 with p-aminobenzoate as substrate
5.2 - 9
-
pH profiles with cofactors acetyl-CoA and 4-nitrophenyl acetate, overview
6.2 - 7.3
-
about half-maximal activity at pH 6.2 and 7.3
6.5 - 9.5
-
80% activity
7 - 9.5
-
80% activity
9.3 - 9.5
-
about half-maximal activity at pH 9.3, maximal at 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
23
-
assay at; assay at
25
P50292
assay at; assay at
25
-
assay at
25
-
activity assay; assay at
25
-
activity assay; assay at
28
-
activity assay; assay at
30
A2V7M5
assay at
30
-
activity assay; assay at
37
-
activity with 2-aminofluorene and 4-aminobenzoate