Information on EC 2.3.1.5 - arylamine N-acetyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
2.3.1.5
-
RECOMMENDED NAME
GeneOntology No.
arylamine N-acetyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and characterization of active site
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and characterization of active site
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
mechanism and inactivation
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
a conserved active site loop is involved in substrate recognition, structure analysis, Ser125 in NAT2 is proximal to the catalytic triad and faces a passageway to the catalytic core
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping pong reaction mechanism, the enzyme contains a strictly conserved a Cys-His-Asp catalytic triad, acetylation of the hamster NAT2 is dependent on a thiolate-imidazolium ion pair, Cys-S--His-ImH+, and not a general acid-base catalysis, a deacetylation mechanism dominated by nucleophilic attack of the thiol ester for arylamines
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping pong reaction mechanism, kinetic mechanism, overview
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
structure-function analysis, Cys68-His107-Asp122 catalytic triad; structure-function analysis, Cys68-His107-Asp122 catalytic triad
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
molecular mechanism of arylamine acetylation by the catalytic triad of NAT1, overview, the catalytic triad is formed by Cys68, His107, and Asp122
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
Cys69, His108, and Asp125 form the catalytic triad
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
steady-state kinetic analysis of TBNAT reveals that the enzyme catalyzes the reaction via a bi-bi ping-pong kinetic mechanism: enzyme initially reacts with AcCoA to generate the first product, CoA, and the acetyl-enzyme intermediate (E-Ac). The amine binds and reacts with E-Ac to generate the second product, acetylated amine, and regenerate the free enzyme. The pH dependence of the kinetic parameters reveals that one enzyme group must be deprotonated for optimal catalytic activity and that two amino acid residues at the active site of the free enzyme are involved in binding and/or catalysis. Solvent kinetic isotope effects suggest that proton transfer steps are not rate-limiting in the overall reaction for substituted aniline substrates but become rate-limiting when poor hydrazide substrates are used
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
it is shown that the formation of a thiolate-imidazolium ion pair by Cys68 and His107 is involved in the catylytic mechanism. Asp122 is required for optimal catalysis and structural stability. Enzyme deacetylation step proceeds via nucleophilic attack for acceptor amines with pKa values less than 5.5 and by deprotonation of a tetrahedral intermediate for amines with pKa values greater than 5.5
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
residue Y190 is critical for maximizing the acetylation rate of NAT2 and the transacetylation rate of substrate p-aminobenzoic acid. Y190 also plays an important role in determining the pKa of the active site Cys during acetylation, as well as the pH versus the rate profile for transacetylation
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
ping-pong bi-bi mechanism
Mus musculus C57BL/67, Rattus norvegicus Sprague-Dawley
-
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
Cys69, His108, and Asp125 form the catalytic triad
Streptomyces griseus IFO13350
-
-
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
-
Acyl group transfer
-
Acyl group transfer
-
-
Acyl group transfer
-
Acyl group transfer
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Caffeine metabolism
-
-
Nitrotoluene degradation
-
-
Drug metabolism - other enzymes
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:arylamine N-acetyltransferase
Wide specificity for aromatic amines, including serotonin; also catalyses acetyl-transfer between arylamines without CoA.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-naphthylamine N-acetyltransferase
-
-
-
-
4-aminobiphenyl N-acetyltransferase
-
-
-
-
acetyl CoA-arylamine N-acetyltransferase
-
-
-
-
acetyltransferase, 2-naphthylamine N-
-
-
-
-
acetyltransferase, 4-aminobiphenyl
-
-
-
-
acetyltransferase, arylamine
-
-
-
-
acetyltransferase, p-aminosalicylate N-
-
-
-
-
acetyltransferase, procainamide N-
-
-
-
-
acetyltransferase, serotonin N-
-
-
-
-
arylamine acetylase
-
-
-
-
arylamine acetyltransferase
-
-
-
-
arylamine N-acetyltransferase
Q6HVT9, Q81PT0, Q81R98
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
Bacillus cereus 10-L-2
-
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
-
arylamine N-acetyltransferase
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
-
-
arylamine N-acetyltransferase
Rhodococcus RHA1
-
-
-
arylamine N-acetyltransferase 1
-
-
arylamine N-acetyltransferase 1
-
-
arylamine N-acetyltransferase 1
-
-
arylamine N-acetyltransferase 1
-
-
arylamine N-acetyltransferase 2
-
arylamine N-acetyltransferase 2
-
-
arylamine N-acetyltransferase 2
-
arylamine N-acetyltransferase 2
-
-
arylamine N-acetyltransferase C
-
-
arylamine N-acetyltransferase C
-
-
arylamine N-acetyltransferase C
-
-
arylamine N-acetyltransferase I
-
-
arylamine N-acetyltransferase type I
-
BanatA
Q6HVT9
-
BanatB
; in contrast to BanatA or BanatC, BanatB also has acetyl-CoA and p-nitrophenyl acetate hydrolysis activity in the absence of arylamine substrates, indicating that it may act also as an AcCoA hydrolase
beta-naphthylamine N-acetyltransferase
-
-
-
-
indoleamine N-acetyltransferase
-
-
-
-
N-acetyltransferase
-
-
-
-
N-acetyltransferase a
-
-
N-acetyltransferase a
Bacillus cereus 10-L-2
-
-
-
N-acetyltransferase b
-
-
N-acetyltransferase b
Bacillus cereus 10-L-2
-
-
-
N-acetyltransferase type 2
-
-
N-hydroxyarylamine O-acetyltransferase
-
-
NAT
-
-
-
-
NAT
Rhodococcus RHA1
-
-
-
NAT
Streptomyces griseus IFO13350
-
-
NAT 1
-
-
NAT-a
Bacillus cereus 10-L-2
-
-
-
NAT-b
Bacillus cereus 10-L-2
-
-
-
NAT1
-
-
-
-
NAT2
-
-
-
-
NAT2
-
equivalent of human NAT1 in terms of tissue distribution, gene organization, C-terminal sequence analysis and substrate specificity
NAT2*1
polymorphic allele Nat2*1, differentiated by one synonymous C624T polymorphism
NAT2*2
polymorphic allele Nat2*2, differentiated by one nonsynonymous G691A polymorphism, resulting in a Val231Ile substitution
NAT3
-
in mice there are three functional polymorphic NAT genes. NAT3 is highly polymorphic
p-aminosalicylate N-acetyltransferase
-
-
-
-
rhesus NAT2
-
-
serotonin acetyltransferase
-
-
-
-
serotonin N-acetyltransferase
-
-
-
-
UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase
-
-
UDP-D-Glc(2NAc3N)A 3-N-acetyltransferase
-
-
additional information
-
WbpD belongs to the hexapeptide acyltransferase superfamily of enzymes
CAS REGISTRY NUMBER
COMMENTARY
9027-33-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Achromobacter xylosoxidans 71.32
strain 71.32
-
-
Manually annotated by BRENDA team
strain 76.14
-
-
Manually annotated by BRENDA team
Aeromonas hydrophila 76.14
strain 76.14
-
-
Manually annotated by BRENDA team
duck
-
-
Manually annotated by BRENDA team
; BanatA
Q6HVT9
UniProt
Manually annotated by BRENDA team
; BanatB
UniProt
Manually annotated by BRENDA team
; BanatC
UniProt
Manually annotated by BRENDA team
distribution among soil microorganisms, overview
-
-
Manually annotated by BRENDA team
strain 10-L-2
-
-
Manually annotated by BRENDA team
Bacillus cereus 10-L-2
strain 10-L-2
-
-
Manually annotated by BRENDA team
strain 100
-
-
Manually annotated by BRENDA team
Bacteroides sp. 100
strain 100
-
-
Manually annotated by BRENDA team
no activity in Canis lupus familiaris
-
-
Manually annotated by BRENDA team
strain 82.89
-
-
Manually annotated by BRENDA team
Citrobacter amalonaticus 82.89
strain 82.89
-
-
Manually annotated by BRENDA team
strain 104553
-
-
Manually annotated by BRENDA team
Citrobacter farmeri 104553
strain 104553
-
-
Manually annotated by BRENDA team
strain 57.32
-
-
Manually annotated by BRENDA team
Citrobacter freundii 57.32
strain 57.32
-
-
Manually annotated by BRENDA team
strain 82.94
-
-
Manually annotated by BRENDA team
Citrobacter koseri 82.94
strain 82.94
-
-
Manually annotated by BRENDA team
insect pathogen fungus
-
-
Manually annotated by BRENDA team
strain N5-36
-
-
Manually annotated by BRENDA team
Enterobacter aerogenes N5-36
strain N5-36
-
-
Manually annotated by BRENDA team
strain 54.8; strain K-12 MG1655
-
-
Manually annotated by BRENDA team
Escherichia coli 54.8
strain 54.8
-
-
Manually annotated by BRENDA team
Escherichia coli K-12 MG1655
strain K-12 MG1655
-
-
Manually annotated by BRENDA team
strain 43579
-
-
Manually annotated by BRENDA team
Helicobacter pylori 43579
strain 43579
-
-
Manually annotated by BRENDA team
genetic polymorphism
-
-
Manually annotated by BRENDA team
human
-
-
Manually annotated by BRENDA team
isoform NAT 1
SwissProt
Manually annotated by BRENDA team
isoform NAT 2
SwissProt
Manually annotated by BRENDA team
isoform NAT2
SwissProt
Manually annotated by BRENDA team
isozyme NAT1
-
-
Manually annotated by BRENDA team
isozyme NAT1; gene NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT1; isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2
-
-
Manually annotated by BRENDA team
isozyme NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
NAT1
-
-
Manually annotated by BRENDA team
NAT1 and NAT2
-
-
Manually annotated by BRENDA team
NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
recombinant NAT2, several genetic variants
-
-
Manually annotated by BRENDA team
two isozymes NAT1, NAT2
-
-
Manually annotated by BRENDA team
strain 103434
-
-
Manually annotated by BRENDA team
Klebsiella oxytoca 103434
strain 103434
-
-
Manually annotated by BRENDA team
strain 82.91
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae 82.91
strain 82.91
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae subsp. ozaenae 52.211
strain 52.211
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae subsp. rhinoscleromatis 52.210
strain 52.210
-
-
Manually annotated by BRENDA team
polymorphic allele Nat2*1; polymorphic allele Nat2*2
Uniprot
Manually annotated by BRENDA team
enzyme form NAT2
-
-
Manually annotated by BRENDA team
isozyme NAT1; isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2; isozyme NAT2
SwissProt
Manually annotated by BRENDA team
recombinant NAT1, NAT2
-
-
Manually annotated by BRENDA team
recombinant protein, fusion with dihydrofolate reductase
-
-
Manually annotated by BRENDA team
recombinant protein, NAT1
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
strain A.231
-
-
Manually annotated by BRENDA team
Morganella morganii A.231
strain A.231
-
-
Manually annotated by BRENDA team
fast acetylator strain C57BL6, slow acetylator strain A/J
-
-
Manually annotated by BRENDA team
isoform NAT1
-
-
Manually annotated by BRENDA team
isozyme NAT1; male mice, isozyme NAT1
SwissProt
Manually annotated by BRENDA team
isozyme NAT2; male mice, isozyme NAT2
SwissProt
Manually annotated by BRENDA team
housefly
-
-
Manually annotated by BRENDA team
K-10, str. 104
-
-
Manually annotated by BRENDA team
; BCG Pasteur, AF2122/97
-
-
Manually annotated by BRENDA team
; strain BCG Pasteur
-
-
Manually annotated by BRENDA team
NAT gene is essential for survival of Mycobacterium bovis
-
-
Manually annotated by BRENDA team
strain Bacille Calmette-Guerin
-
-
Manually annotated by BRENDA team
strain BCG, gene nat
-
-
Manually annotated by BRENDA team
Mycobacterium bovis Bacille
strain Bacille Calmette-Guerin
-
-
Manually annotated by BRENDA team
; CDC1551, Haarlem, F11, C, H73Rv
-
-
Manually annotated by BRENDA team
no activity in Canis familiaris
-
-
-
Manually annotated by BRENDA team
new Zealand white
-
-
Manually annotated by BRENDA team
new Zealand white; rapid acetylator type III/J
-
-
Manually annotated by BRENDA team
slow and fast acetylator phenotype
-
-
Manually annotated by BRENDA team
strain 103286
-
-
Manually annotated by BRENDA team
Pasteurella multocida 103286
strain 103286
-
-
Manually annotated by BRENDA team
Plesiomonas shigelloides 63.5
strain 63.5
-
-
Manually annotated by BRENDA team
strain 58.60
-
-
Manually annotated by BRENDA team
Proteus vulgaris 58.60
strain 58.60
-
-
Manually annotated by BRENDA team
Providencia alcalifaciens 82.90
strain 82.90
-
-
Manually annotated by BRENDA team
strain 100720
-
-
Manually annotated by BRENDA team
strain ATCC 27853
-
-
Manually annotated by BRENDA team
strain PAO1, serotype O5, gene wbpD
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa 100720
strain 100720
-
-
Manually annotated by BRENDA team
hepatocyte primary culture
-
-
Manually annotated by BRENDA team
isozyme Nat3; inbred strains F344, WKY, and SPRD, isozymes Nat1, Nat2, and Nat3
SwissProt
Manually annotated by BRENDA team
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
RHA1
-
-
Manually annotated by BRENDA team
Rhodococcus RHA1
RHA1
-
-
Manually annotated by BRENDA team
strain YG1024, strain TA98 1,8/DNP, strain TA1538, strain TA98, strain 60.62
-
-
Manually annotated by BRENDA team
strain 103235
-
-
Manually annotated by BRENDA team
Serratia marcescens 103235
strain 103235
-
-
Manually annotated by BRENDA team
strain 82.48
-
-
Manually annotated by BRENDA team
Shigella flexneri 82.48
strain 82.48
-
-
Manually annotated by BRENDA team
strain IFO13350, gene natA
SwissProt
Manually annotated by BRENDA team
Streptomyces griseus IFO13350
strain IFO13350, gene natA
SwissProt
Manually annotated by BRENDA team
strain 54.315
-
-
Manually annotated by BRENDA team
Vibrio cholerae 54.315
strain 54.315
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
knockdown of N-acetyltransferase by siRNA results in downregulation of thymidylate synthase mRNA expression and induced apoptosis. N-acetyltransferase overexpression facilitates cell proliferation independent of the presence of 5-fluorouracil
physiological function
-
knock-down of enzyme by lentiviral shRNA reduces the invasion potential of MDA-MB-231 cells. Enzymic activity may be important in breast cancer growth and metastasis
physiological function
-
increasing acetylation by introduction of the human NAT1 transgene is protective against sporadic dilantin-induced orofacial clefting defects in the mouse A/J strain
physiological function
-
knockout of NAT2 significantly decreases teratogen-induced orofacial clefting in the A/J strain, but not in the C57BL/6J strain
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + 2-aminofluorene
4-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 2-aminofluorene
4-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
substrate of isozyme NAT1, very low activity with isozyme NAT2
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
a sunscreen additive, substrate of isozyme NAT1, very low activity with isozyme NAT2
-
?
4-nitrophenyl acetate + 4-aminobenzoyl glutamic acid
4-nitrophenol + N-acetyl-4-aminobenzoyl glutamic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoyl glutamic acid
4-nitrophenol + N-acetyl-4-aminobenzoyl glutamic acid
show the reaction diagram
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
a tobacco smoke carcinogen, substrate of isozyme NAT1, very low activity with isozyme NAT2
-
?
4-nitrophenyl acetate + 4-anisidine
4-nitrophenol + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-nitroaniline
4-nitrophenol + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
?
4-nitrophenyl acetate + 5-aminosalicylic acid
4-nitrophenol + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 5-aminosalicylic acid
4-nitrophenol + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + aniline
4-nitrophenol + N-acetyl-aniline
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + anisidine
4-nitrophenol + N-acetyl-anisidine
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + anisidine
4-nitrophenol + N-acetyl-anisidine
show the reaction diagram
-
-
?
4-nitrophenyl acetate + hydralazine
4-nitrophenol + N-acetyl-hydralazine
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + isoniazid
4-nitrophenol + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + (1-methyl-5-piperazin-1-yl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7-yl)-(5-methyl-pyridin-2-yl)-amine
CoA + ?
show the reaction diagram
-
i.e. UK-469,413. Acetylation by isozyme NAT2 in liver cytosol to N-acetylpiperazine metabolite
-
?
acetyl-CoA + (1-methyl-5-piperazin-1-yl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7-yl)-(5-methyl-pyridin-2-yl)-amine
CoA + ?
show the reaction diagram
-
i.e. UK-469,413. Rapid acetylation in liver cytosol to N-acetylpiperazine metabolite
-
?
acetyl-CoA + 2,3-dimethylaniline
CoA + N-acetyl-2,3-dimethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 2,4-dimethylaniline
CoA + N-acetyl-2,4-dimethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 2,5-dimethylaniline
CoA + N-acetyl-2,5-dimethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-(4-aminobenzamido)pyridine
CoA + 2-(4-acetylamidobenzamido)pyridine
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + 2-acetylamino-4-chlorophenol
show the reaction diagram
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + N-(5-chloro-2-hydroxyphenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-amino-4-chlorophenol
CoA + N-acetyl-2-amino-4-chlorophenol
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + 2-acetylamino-4-methylphenol
show the reaction diagram
best substrate
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + N-(2-hydroxy-5-methylphenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-amino-4-methylphenol
CoA + N-acetyl-2-amino-4-methylphenol
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetylanthranilate
show the reaction diagram
-
i.e. anthranilate, ir
-
ir
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetyl-2-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminobenzoic acid
CoA + N-acetyl-2-aminobenzoic acid
show the reaction diagram
-
relative activity: 0%
-
?
acetyl-CoA + 2-aminobiphenyl
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
N-acetyltransferase 2
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
NAT1, poor substrate
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
interactions and substrate binding structure, overview
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
high specific activity
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
-
mutant Y71F, 128% of wild-type activity, mutant M209T, 62% of wild-type activity
-
?
acetyl-CoA + 2-aminofluorene
CoA + N-acetyl-2-aminofluorene
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
?
acetyl-CoA + 2-aminofluorene
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + 2-acetylaminophenol
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-(2-hydroxyphenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-acetyl-2-aminophenol
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + N-acetyl-2-aminophenol
show the reaction diagram
-
relative activity: 36%, relative activity: 72%
-
?
acetyl-CoA + 2-chloroaniline
CoA + N-acetyl-2-chloroaniline
show the reaction diagram
-
relative activity: 12%, relative activity: 18%
-
?
acetyl-CoA + 2-ethylaniline
CoA + N-acetyl-2-ethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-methylaniline
CoA + N-acetyl-2-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-nitroaniline
CoA + N-acetyl-2-nitroaniline
show the reaction diagram
-
relative activity: 0%, relative activity: 3%
-
?
acetyl-CoA + 2-phenylendiamine
CoA + ?
show the reaction diagram
-
relative activity: 80%, relative activity: 90%
-
?
acetyl-CoA + 2-toluidine
CoA + N-acetyl-2-toluidine
show the reaction diagram
-
relative activity: 0%
-
?
acetyl-CoA + 3,4-dichloraniline
CoA + N-acetyl-3,4-dichloraniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,4-dichloroaniline
CoA + N-acetyl-3,4-dichloroaniline
show the reaction diagram
-
mutant Y71F, 194% of wild-type activity, mutant M209T, 88% of wild-type activity
-
?
acetyl-CoA + 3,4-dimethylaniline
CoA + N-acetyl-3,4-dimethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,5-dimethyl-4-nitroaniline
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,5-dimethylaniline
CoA + N-acetyl-3,5-dimethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3,5-dimethylaniline
CoA + N-acetyl-3,5-dimethylaniline
show the reaction diagram
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
an intermediate of grixazone synthesis
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
Streptomyces griseus IFO13350
an intermediate of grixazone synthesis
-
?
acetyl-CoA + 3-amino-4-hydroxybenzenesulfonic acid
CoA + 3-acetylamino-4-hydroxybenzenesulfonic acid
show the reaction diagram
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
best substrate, an intermediate of grixazone synthesis
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
-
an intermediate of grixazone synthesis
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
Streptomyces griseus IFO13350
best substrate
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-(acetylamino)-4-hydroxybenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + N-acetyl-3-amino-4-hydroxybenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
-
relative activity: 105%, relative activity: 122%
-
?
acetyl-CoA + 3-aminobenzoic acid
CoA + N-acetyl-3-aminobenzoic acid
show the reaction diagram
Bacillus cereus 10-L-2
-
relative activity: 105%, relative activity: 122%
-
?
acetyl-CoA + 3-aminophenol
CoA + N-acetyl-3-aminophenol
show the reaction diagram
-
relative activity: 33%, relative activity: 56%
-
?
acetyl-CoA + 3-chloraniline
CoA + N-acetyl-3-chloroaniline
show the reaction diagram
-
relative activity: 48%
-
?
acetyl-CoA + 3-ethylaniline
CoA + N-acetyl-3-ethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 3-ethylaniline
CoA + N-acetyl-3-ethylaniline
show the reaction diagram
-
-
?
acetyl-CoA + 3-nitroaniline
CoA + N-acetyl-3-nitroaniline
show the reaction diagram
-
relative activity: 2%, relative activity: 6%
-
?
acetyl-CoA + 3-phenylendiamine
CoA + ?
show the reaction diagram
-
relative activity: 0%
-
?
acetyl-CoA + 3-toluidine
CoA + N-acetyl-3-toluidine
show the reaction diagram
-
relative activity: 21%, relative activity: 28%
-
?
acetyl-CoA + 4,4'-methylenebis(2-chloroaniline)
CoA + N-acetyl-4,4'-methylenebis(2-chloroaniline)
show the reaction diagram
-
-
?
acetyl-CoA + 4,4'-methylenedianiline
CoA + N-acetyl-4,4'-methylenedianiline
show the reaction diagram
-
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + 4-acetylamino-3-hydroxybenzoic acid
show the reaction diagram
low activity
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + 4-(acetylamino)-3-hydroxybenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-amino-3-hydroxybenzoic acid
CoA + N-acetyl-4-amino-3-hydroxybenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-amino-4'-nitroazobenzene
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoacetanilide
CoA + N-acetyl-4-aminoacetanilide
show the reaction diagram
-
relative activity: 0%
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobenzoate
CoA + 4-acetylaminobenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
poor
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
N-acetyltransferase 1
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
-
the catalytic mechanism depends on the formation of a thiolate-imidazolium ion pair (Cys-S-)-(His-ImH+)
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-acetylaminobenzoic acid
show the reaction diagram
Enterobacter aerogenes N5-36
-
poor
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
preferred substrate
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
isozyme NAT1
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
high specific activity
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
relative activity: 19%, relative activity: 26%
-
?
acetyl-CoA + 4-aminobenzoic acid
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoic acid
CoA + 4-(acetylamino)benzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamate
CoA + 4-acetylaminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamate
CoA + N-acetyl-4-aminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobenzoyl-L-glutamic acid
CoA + 4-acetylaminobenzoyl-L-glutamic acid
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
substrate of isozyme NAT2 in liver cytosol
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
substrate of isozyme NAT2
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + 4-acetylaminobiphenyl
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminobiphenyl
CoA + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminofluorene
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminophenol
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminophenol
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminophenol
CoA + N-acetyl-4-aminophenol
show the reaction diagram
-
relative activity: 48%, relative activity: 94%
-
?
acetyl-CoA + 4-aminopyridine
?
show the reaction diagram
-
very low activity
-
-
?
acetyl-CoA + 4-aminopyridine
?
show the reaction diagram
very low activity
-
-
?
acetyl-CoA + 4-aminopyridine
CoA + N-acetyl-4-aminopyridine
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminosalicylate
CoA + N-acetyl-4-aminosalicylate
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
?
acetyl-CoA + 4-aminosalicylate
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
N-acetyltransferase 1
-
-
acetyl-CoA + 4-aminosalicylic acid
CoA + 4-acetylamino-2-hydroxybenzoate
show the reaction diagram
Mus musculus C57BL/67
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
high specific activity
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
relative activity: 100%, relative activity: 90%
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
mutant Y71F, 17% of wild-type activity, mutant M209T, 128% of wild-type activity
-
?
acetyl-CoA + 4-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + N-acetyl-4-aminoveratrole
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
-
-
?
acetyl-CoA + 4-aminoveratrole
CoA + 1,2-dimethoxy-4-acetylaminobenzene
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-aminoveratrole
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-aminoveratrole
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + N-acetyl-4-anisidine
show the reaction diagram
-
mutant Y71F, 860% of wild-type activity, mutant M209T, 673% of wild-type activity
-
?
acetyl-CoA + 4-anisidine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-anisidine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromaniline
CoA + N-acetyl-4-bromaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + 4-bromoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-(4-bromophenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-acetyl-4-bromoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-bromoaniline
CoA + N-acetyl-4-bromoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
CoA + N-acetyl-4-butoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-butoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + 4-chloroacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
relative activity: 18%, relative activity: 21%
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-acetyl-4-chloroaniline
show the reaction diagram
-
mutant Y71F, 174% of wild-type activity, mutant M209T, 122% of wild-type activity
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-(4-chlorophenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chloroaniline
CoA + N-(4-chlorophenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N'-acetyl-(4-chlorobenzo)-hydrazide
show the reaction diagram
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N-acetyl-4-chlorobenzoic hydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-chlorobenzoic hydrazide
CoA + N-acetyl-4-chlorobenzoic hydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-dimethylaminobenzaldehyde
CoA + 5-acetyl-4-dimethylaminobenzaldehyde
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + 4-ethoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
CoA + N-acetyl-4-ethoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-ethylaniline
CoA + 4-ethylacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-ethylaniline
CoA + N-acetyl-4-ethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-fluoroaniline
CoA + N-(4-fluorophenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-fluoroaniline
CoA + N-acetyl-4-fluoroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + 4-hexyloxyacetanilide
show the reaction diagram
best substrate
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
CoA + N-acetyl-4-hexyloxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hexyloxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-hydroxybenzhydrazide
CoA + N'-acetyl-4-hydroxybenzohydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-hydroxybenzhydrazide
CoA + N-acetyl-4-hydroxybenzhydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-acetyl-4-iodoaniline
show the reaction diagram
-
mutant Y71F, 196% of wild-type activity, mutant M209T, 83% of wild-type activity
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + 4-iodoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-iodoaniline
CoA + N-(4-iodophenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methoxyaniline
CoA + N-acetyl-4-methoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + 4-methoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methoxyphenylhydrazine
CoA + N-acetyl-4-methoxyphenylhydrazine
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-acetyl-4-methylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-methylaniline
CoA + N-(4-methylphenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
-
-
?
acetyl-CoA + 4-nitroaniline
CoA + N-acetyl-4-nitroaniline
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
relative activity: 0%
-
?
acetyl-CoA + 4-nitrobenzalhydrazone
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + 4-phenoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
CoA + N-acetyl-4-phenoxyaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-phenoxyaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-phenylenediamine
CoA + 4-aminoacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-toluidine
CoA + N-acetyl-4-toluidine
show the reaction diagram
-
relative activity: 40%, relative activity: 80%
-
?
acetyl-CoA + 4-trifluoromethylaniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + 4-trifluoromethylaniline
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 4-trifluoromethylaniline
CoA + N-acetyl-4-trifluoromethylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
?
acetyl-CoA + 5-aminosalicylate
CoA + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylate
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylate
?
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylamino-2-hydroxybenzoate
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
high specific activity
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
relative activity: 510%, relative activity: 550%
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
-
mutant Y71F, 147% of wild-type activity, mutant M209T, 21% of wild-type activity
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + N-acetyl-5-aminosalicylic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
poor substrate
-
?
acetyl-CoA + 5-methoxytryptamine
CoA + N-acetyl-5-methoxytryptamine
show the reaction diagram
-
poor substrate
-
?
acetyl-CoA + 8-aminoisoindolo (1,2-b)quinazolin-12(10H)-one
?
show the reaction diagram
-
batricylin, an antitumor agent, is shown to be a substrate for NAT2
-
-
-
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
involved in metabolism of drugs and endogenous substances containing an amine or hydrazine group
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
pineal gland: inducible
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
expression of NAT1 in muscle cells may be an important factor in the detoxification/activation process because of the potential involvement of the muscle in the pharmacokinetics of many xenobiotics
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
important role in detoxification and metabolic activation of a variety of aromatic xenobiotics, including numerous carcinogens. Cellular generation of peroxinitrite may contribute to carcinogenesis and tumor progression by weakening key cellular defense enzymes such as NAT1
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
constitutive enzyme, pineal gland: inducible
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
endogenous role of the enzyme in the protection of bacteria from aromatic and lipophilic toxins
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
catalytic pathway, overview
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
NAT2 is responsible for the biotransformation of numerous arylamine drugs and carcinogens
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are important enzymes involved in the metabolic activation of aromatic and heterocyclic amines
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
the enzyme catalyzes the N-acetylation of arylamines and hydrazines and O-acetylation of N-hydroxy-arylamines and heterocyclic amines
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an N-hydroxyarylamine
CoA + an N-acetoxyarylamine
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetylaniline
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + acetanilide
show the reaction diagram
Enterobacter aerogenes N5-36
-
-
-
?
acetyl-CoA + aniline
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
-
-
?
acetyl-CoA + aniline
CoA + N-acetyl-aniline
show the reaction diagram
-
relative activity: 12%, relative activity: 24%
-
?
acetyl-CoA + aniline
CoA + N-phenylacetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
-
-
-
?
acetyl-CoA + anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
-
-
?
acetyl-CoA + anisidine
CoA + N-(4-methoxyphenyl)acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + benzidine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + benzidine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + benzidine
CoA + N-acetyl-benzidine
show the reaction diagram
-
-
?
acetyl-CoA + benzoic acid hydrazide
CoA + N'-acetylbenzohydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + benzoic acid hydrazide
CoA + N-acetyl-benzoic acid hydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + beta-naphthylamine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + beta-naphthylamine
CoA + N-acetyl-beta-naphthylamine
show the reaction diagram
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
-
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
poor substrate
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
-
not chicken pineal gland and brain
-
?
acetyl-CoA + beta-phenylethylamine
CoA + N-(2-phenylethyl)-acetaminde
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
acetyl-CoA + butoxyaniline
CoA + 4-butoxyacetanilide
show the reaction diagram
-
-
-
?
acetyl-CoA + D-glucosamine
CoA + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
acetyl-CoA + decylamine
CoA + N-acetyldecylamine
show the reaction diagram
-
-
-
-
acetyl-CoA + decylamine
CoA + N-acetyldecylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + diaminodiphenylsulfone
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + diaminodiphenylsulfone
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + diaminodiphenylsulfone
monoacetyldiaminodiphenylsulfone + CoA
show the reaction diagram
-
i.e. dapsone, predominantly acetylated by NAT2
-
?
acetyl-CoA + hexylamine
CoA + N-acetylhexylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + histamine
CoA + N-acetylhistamine
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
?
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetylhydralazine
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + N'-phthalazin-1-ylacetohydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
?
acetyl-CoA + hydralazine
CoA + N-acetyl-hydralazine
show the reaction diagram
-
low specific activity
-
?
acetyl-CoA + hydroxytyramine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetyl-isoniazid
show the reaction diagram
-
low specific activity
-
?
acetyl-CoA + isoniazid
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N'-acetylbenzohydrazide
show the reaction diagram
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
-
mutant Y71F, 102% of wild-type activity, mutant M209T, 229% of wild-type activity
-
?
acetyl-CoA + isoniazid
CoA + an N-acetylisoniazid
show the reaction diagram
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazid
?
show the reaction diagram
inactivation of the anti-tubercular drug isoniazid by acetyltransfer
-
-
?
acetyl-CoA + isoniazid
CoA + N'-acetylpyridine-4-carbohydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
-
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
i.e. isonicotinyl hydrazide
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
-
isoniazide binds the enzyme prior to acetyl-CoA
-
?
acetyl-CoA + isoniazide
CoA + acetylniazide
show the reaction diagram
Mycobacterium bovis Bacille
-
-
-
?
acetyl-CoA + long-chain primary amines
CoA + ?
show the reaction diagram
-
C6-C16
-
?
acetyl-CoA + methyl bis-2-chloroaniline
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + N,N-1,4-phenylenediamine
CoA + ?
show the reaction diagram
-
relative activity: 100%
-
?
acetyl-CoA + N-(4-acetylaminobenzoyl)-L-glutamate
CoA + 4-acetylaminobenzoyl-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + N-(4-aminobenzoyl)-L-glutamate
CoA + N-(4-acetylaminobenzoyl)-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-acetyl-N-hydroxy-4-aminobiphenyl + CoA
show the reaction diagram
substrate of isozyme NAT2 in liver cytosol
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
O-acetylation activity
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-hydroxy-O-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
substrate of isozyme NAT2, O-acetylation activity of NAT2
-
?
acetyl-CoA + naphthylamine
CoA + N-acetylnaphthylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + nicotinic acid hydrazide
CoA + N'-acetylpyridine-3-carbohydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + nicotinic acid hydrazide
CoA + N-acetyl-nicotinic acid hydrazide
show the reaction diagram
-
-
-
?
acetyl-CoA + noradrenaline
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + normetanephrine
CoA + N-acetylnormetanephrine
show the reaction diagram
-
-
-
?
acetyl-CoA + octylamine
CoA + N-acetyloctylamine
show the reaction diagram
-
-
-
-
acetyl-CoA + octylamine
CoA + N-acetyloctylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
poor activity
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-p-aminobenzoic acid
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
?
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + 4-(acetylamino)benzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
activity assay
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
activity assay
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
in vitro N-acetylation assay
-
?
acetyl-CoA + p-aminobenzoic acid
CoA + N-acetyl-aminobenzoic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminobenzoylglutamate
CoA + N-[(4-acetylamino)]benzoyl-L-glutamate
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminosalicylate
CoA + N-acetyl-p-aminosalicylate
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-aminosalicylic acid
CoA + N-acetyl-4-aminosalicylic acid
show the reaction diagram
-
-
-
?
acetyl-CoA + p-anisidine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + p-anisidine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + p-anisidine
CoA + N-acetyl-anisidine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-anisidine
CoA + p-methoxyacetanilide
show the reaction diagram
-
-
?
acetyl-CoA + p-anisidine
CoA + N-acetylanisidine
show the reaction diagram
-
-
?
acetyl-CoA + p-octopamine
CoA + N-acetyloctopamine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-octopamine
CoA + N-acetyloctopamine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-octopamine
CoA + N-acetyloctopamine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + p-phenetidine
CoA + N-(4-ethoxyphenyl)-acetamide
show the reaction diagram
-
i.e. 4-ethoxybenzenamine, best substrate
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
-
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
-
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
-
ie. 4-(2-aminoethyl)phenol
-
?
acetyl-CoA + p-tyramine
CoA + N-acetyl-p-tyramine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
acetyl-CoA + peptide
CoA + Nalpha-acetylpeptide
show the reaction diagram
-
-
-
?
acetyl-CoA + phenelzine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + phenylhydrazine
CoA + N-acetyl-phenylhydrazine
show the reaction diagram
-
low specific activity
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
N-acetyltransferase 2
-
?
acetyl-CoA + procainamide
CoA + N-acetylprocainamide
show the reaction diagram
-
N-acetyltransferase 2
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
?
acetyl-CoA + procainamide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
lower activity
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-2-procainamide
show the reaction diagram
Escherichia coli 54.8, Aeromonas hydrophila 76.14, Klebsiella pneumoniae subsp. rhinoscleromatis 52.210, Citrobacter amalonaticus 82.89, Citrobacter freundii 57.32, Vibrio cholerae 54.315, Bacteroides sp. 100, Escherichia coli K-12 MG1655, Providencia alcalifaciens 82.90, Klebsiella pneumoniae subsp. ozaenae 52.211, Klebsiella pneumoniae 82.91, Helicobacter pylori 43579, Pseudomonas aeruginosa 100720, Plesiomonas shigelloides 63.5, Morganella morganii A.231, Pasteurella multocida 103286, Klebsiella oxytoca 103434, Shigella flexneri 82.48, Citrobacter koseri 82.94, Citrobacter farmeri 104553, Serratia marcescens 103235, Proteus vulgaris 58.60, Achromobacter xylosoxidans 71.32
-
-
-
?
acetyl-CoA + procainamide
CoA + N-acetyl- procainamide
show the reaction diagram
-
low specific activity
-
?
acetyl-CoA + procainamide
CoA + N-acetyl-procainamide
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
-
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
-
-
-
?
acetyl-CoA + serotonin
CoA + N-acetylserotonin
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfadiazine
CoA + N-acetylsulfadiazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamerazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamerazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamerazine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
N-acetyltransferase 2
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
-
N-acetyltransferase 2
-
?
acetyl-CoA + sulfamethazine
CoA + N-4-acetylsulfamethazine
show the reaction diagram
Bacillus cereus 10-L-2
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
-
very low activity
-
-
?
acetyl-CoA + sulfamethazine
?
show the reaction diagram
very low activity
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
isozyme NAT2
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
low specific activity
-
?
acetyl-CoA + sulfamethazine
CoA + N-acetyl-sulfamethazine
show the reaction diagram
-
relative activity: 0%, relative activity: 46%
-
?
acetyl-CoA + sulfamethoxazole
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-acetyl-sulfamethoxazole
show the reaction diagram
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-acetyl-sulfamethoxazole
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfamethoxazole
?
show the reaction diagram
Q6HVT9, Q81PT0, Q81R98
-
-
-
?
acetyl-CoA + sulfamethoxazole
CoA + N-[4-[(5-methylisoxazol-3-yl)sulfamoyl]phenyl]acetamide
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfanilamide
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfanilamide
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulfapyridine
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + sulphamethazine
CoA + N-acetylsulphamethazine
show the reaction diagram
-
-
?
acetyl-CoA + sulphamethoxazole
CoA + N-acetylsulphamethoxazole
show the reaction diagram
-
-
-
?
acetyl-CoA + sulphisoxazole
CoA + 4-acetylamino-N-(3,4-dimethylisoxazol-5-yl)-benzenesulfonamide
show the reaction diagram
-
-
-
?
acetyl-CoA + sulphisoxazole
CoA + N-acetylsulphisoxazole
show the reaction diagram
-
-
-
?
acetyl-CoA + tetradecylamine
CoA + N-acetyltetradecylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + thiazolsulfone
CoA + ?
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
-
-
-
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
-
not chicken pineal gland and brain
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
acetyl-CoA + tryptamine
CoA + N-acetyltryptamine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
?
N-hydroxy-2-acetylaminofluorene + 4-aminoazobenzene
CoA + ?
show the reaction diagram
-
much better acetyl donor for NAT1 than for NAT2
-
?
p-nitroacetanilide + aniline
p-nitroaniline + acetanilide
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + 2-aminofluorene
p-nitrophenol + N-acetyl-2-aminofluorene
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + 5-aminosalicylic acid
p-nitrophenol + 5-acetylaminosalicylic acid
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + aniline
p-nitrophenol + N-acetyl-aniline
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + hydralazine
p-nitrophenol + N-acetyl-hydralazine
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + isoniazid
p-nitrophenol + N-acetyl-isoniazid
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + p-aminobenzoic acid
p-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
p-nitrophenyl acetate + p-anisidine
p-nitrophenol + N-acetyl-4-anisidine
show the reaction diagram
-
-
-
?
p-nitrophenylacetate + 2-aminofluorene
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + 5-aminosalicylic acid
4-nitrophenol + N-acetyl-5-aminosalicylate
show the reaction diagram
-
-
-
?
p-nitrophenylacetate + aniline
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + hydralazine
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + isoniazid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-aminobenzoic acid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-aminosalicylic acid
?
show the reaction diagram
-
-
-
-
?
p-nitrophenylacetate + p-anisidine
?
show the reaction diagram
-
-
-
-
?
sulphamethazine + acetyl-CoA
N-[4-[(4,6-dimethylpyrimidin-2-yl)sulfamoyl]phenyl]acetamide
show the reaction diagram
-
-
?
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
CoA + UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid
show the reaction diagram
-
WbpD is a putative 3-N-acetyltransferase that has been implicated in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, a precursor for the D-Man(2NAc3NAc)A residues in the B-band O antigen of Pseudomonas aeruginosa, the enzyme is involved in the catalysis of the fourth step by acting as an UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase, WbpD utilizes a direct-transfer reaction mechanism
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
the enzyme exhibits arylhydroxamic acid N,O-acetyltransferase activity
-
-
-
additional information
?
-
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
-
N-diacetyl cysteamine can replace acetyl-CoA with less than 10% efficiency
-
-
-
additional information
?
-
-
no substrate: o-nitroaniline
-
-
-
additional information
?
-
-
no substrate: o-nitroaniline
-
-
-
additional information
?
-
-
not butyryl-CoA, no substrate: glucosamine 6-phosphate
-
-
-
additional information
?
-
-
no substrate: phenylalanine, cyclohexylamine
-
-
-
additional information
?
-
-
no substrate: polyamines, amino acids, sphingosine bases, ethanolamine, C1-C4 primary amines, some secondary and tertiary amines
-
-
-
additional information
?
-
-
no substrate: 2-methyl-4-nitroaniline, 2,4-dinitroaniline, 4-nitrophenylhydrazine, carbopyridine
-
-
-
additional information
?
-
-
4-nitroacetanilide, 4-nitrophenylacetate can replace acetyl-CoA
-
-
-
additional information
?
-
-
propionyl-CoA can replace acetyl-CoA
-
-
-
additional information
?
-
-
no substrate: 2-amino-6-nitropyridine
-
-
-
additional information
?
-
-
acetylthiocholine can replace acetyl-CoA
-
-
-
additional information
?
-
-
no expression of NAT2
-
-
-
additional information
?
-
-
the enzyme is required for synthesis of mycolic acids and complex lipids
-
-
-
additional information
?
-
-
xenobiotic metabolizing enzyme. Catalyzes the biotransformation of primary aromatic amine or hydrazine drugs and carcinogens. In addition to genetically controlled interindividual variations in NAT1 activity, oxidative stress and cellular redox status may also regulate NAT1 activity
-
-
-
additional information
?
-
-
no acetylation of sulfamethazine and procainamide
-
-
-
additional information
?
-
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 is not increased in breast tumors compared to healthy tissue, the grade of malignancy is positively related with NAT1
-
-
-
additional information
?
-
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 is increased in breast tumors compared to healthy tissue, the grade of malignancy is negatively associated with NAT2
-
-
-
additional information
?
-
-
NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates
-
-
-
additional information
?
-
the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics
-
-
-
additional information
?
-
-
the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens
-
-
-
additional information
?
-
-
the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate
-
-
-
additional information
?
-
the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview
-
-
-
additional information
?
-
the isozymes catalyze the N-acetylation of aromatic amine and hydrazine drugs and carcinogens, after N-hydroxylation, they also catalyze the metabolic activation of N-hydroxy-arylamines via O-acetylation
-
-
-
additional information
?
-
-
variable expression of isozyme NAT1 due to genetic polymorphism, gene regulation or environmental influences is associated with individual susceptibility to various cancers, overview
-
-
-
additional information
?
-
4-aminobenzoic acid is the preferred substrate
-
-
-
additional information
?
-
-
NAT acceptor substrate specificity, overview
-
-
-
additional information
?
-
-
poor activity wit 2-toluidine and other arylamines linked to bladder cancer
-
-
-
additional information
?
-
-
poor activity with 2-toluidine and other arylamines linked to bladder cancer
-
-
-
additional information
?
-
substrate specificities of isozymes Nat1-Nat3, overview
-
-
-
additional information
?
-
substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview
-
-
-
additional information
?
-
-
enzyme follows a Ping Pong Bi Bi kinetic mechanism
-
-
-
additional information
?
-
-
mouse Nat2 is very similar to human NAT1 in substrate specificity
-
-
-
additional information
?
-
-
results confirm that substituted anilines are excellent substrates and that TBNAT is ineffective in hydrazide N-acetylation
-
-
-
additional information
?
-
Mus musculus C57BL/67
-
the reaction proceeds via a covalent acetyl-enzyme intermediate
-
-
-
additional information
?
-
Streptomyces griseus IFO13350
substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview
-
-
-
additional information
?
-
Enterobacter aerogenes N5-36
-
not butyryl-CoA, no substrate: glucosamine 6-phosphate, propionyl-CoA can replace acetyl-CoA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobenzoic acid
4-nitrophenol + N-acetyl-4-aminobenzoic acid
show the reaction diagram
-
a sunscreen additive
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
-
-
?
4-nitrophenyl acetate + 4-aminobiphenyl
4-nitrophenol + N-acetyl-4-aminobiphenyl
show the reaction diagram
-
a tobacco smoke carcinogen
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminofluorene
CoA + 2-acetylaminofluorene
show the reaction diagram
-
-
-
?
acetyl-CoA + 2-aminophenol
CoA + 2-acetylaminophenol
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
A2V7M5
-
-
?
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde
CoA + 3-acetylamino-4-hydroxybenzaldehyde
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
A2V7M5
an intermediate of grixazone synthesis
-
?
acetyl-CoA + 3-amino-4-hydroxybenzoic acid
CoA + 3-acetylamino-4-hydroxybenzoic acid
show the reaction diagram
A2V7M5
an intermediate of grixazone synthesis
-
?
acetyl-CoA + 4-aminobiphenyl
N-acetyl-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
?
acetyl-CoA + 5-aminosalicylic acid
CoA + 5-acetylaminosalicylic acid
show the reaction diagram
A2V7M5
-
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
constitutive enzyme
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
involved in metabolism of drugs and endogenous substances containing an amine or hydrazine group
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
pineal gland: inducible
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
expression of NAT1 in muscle cells may be an important factor in the detoxification/activation process because of the potential involvement of the muscle in the pharmacokinetics of many xenobiotics
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
-
important role in detoxification and metabolic activation of a variety of aromatic xenobiotics, including numerous carcinogens. Cellular generation of peroxinitrite may contribute to carcinogenesis and tumor progression by weakening key cellular defense enzymes such as NAT1
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50293
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P18440
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P50294, P50295
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
Q80SX3
-
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
O86309
endogenous role of the enzyme in the protection of bacteria from aromatic and lipophilic toxins
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
catalytic pathway, overview
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245
NAT2 is responsible for the biotransformation of numerous arylamine drugs and carcinogens
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are important enzymes involved in the metabolic activation of aromatic and heterocyclic amines
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
NATs are xenobiotic metabolizing enzymes responsible for the acetylation of many arylamine and heterocyclic amines, they therefore play an important role in the detoxification and activation of numerous drugs and carcinogens, regulation, overview
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
P11245, P18440
the enzyme is important for the activation and deactivation of exocyclic amine-containing pro-carcinogens, and for the metabolism of some pharmaceutical drugs
-
?
acetyl-CoA + an arylamine
CoA + a N-acetylarylamine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
constitutive enzyme, pineal gland: inducible
-
?
acetyl-CoA + an arylamine
CoA + an N-acetylarylamine
show the reaction diagram
-
-
-
?
acetyl-CoA + an N-hydroxyarylamine
CoA + an N-acetoxyarylamine
show the reaction diagram
Bacillus cereus, Bacillus cereus 10-L-2
-
-
-
?
acetyl-CoA + isoniazid
CoA + N-acetylisoniazid
show the reaction diagram
O86309
-
-
?
acetyl-CoA + N-hydroxy-4-aminobiphenyl
N-acetyl-N-hydroxy-4-aminobiphenyl + CoA
show the reaction diagram
P50294, P50295
substrate of isozyme NAT2 in liver cytosol
-
?
acetyl-CoA + peptide
CoA + Nalpha-acetylpeptide
show the reaction diagram
-
-
-
?
acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid
CoA + UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronic acid
show the reaction diagram
-
WbpD is a putative 3-N-acetyltransferase that has been implicated in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid, a precursor for the D-Man(2NAc3NAc)A residues in the B-band O antigen of Pseudomonas aeruginosa, the enzyme is involved in the catalysis of the fourth step by acting as an UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase
-
?
additional information
?
-
-
no expression of NAT2
-
-
-
additional information
?
-
-
the enzyme is required for synthesis of mycolic acids and complex lipids
-
-
-
additional information
?
-
-
xenobiotic metabolizing enzyme. Catalyzes the biotransformation of primary aromatic amine or hydrazine drugs and carcinogens. In addition to genetically controlled interindividual variations in NAT1 activity, oxidative stress and cellular redox status may also regulate NAT1 activity
-
-
-
additional information
?
-
P50292
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT1 is not increased in breast tumors compared to healthy tissue, the grade of malignancy is positively related with NAT1
-
-
-
additional information
?
-
P50292
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 acetylates and detoxifies arylamine carcinogens
-
-
-
additional information
?
-
-
isozyme NAT2 is increased in breast tumors compared to healthy tissue, the grade of malignancy is negatively associated with NAT2
-
-
-
additional information
?
-
-
NAT plays a key role in the metabolic activation of aromatic amine and nitroaromatic mutagens to electrophilic reactive intermediates
-
-
-
additional information
?
-
P50293
the enzyme catalyzes an acetyl group transfer from AcCoA to primary arylamines, hydrazines, and hydrazides and play a very important role in the metabolism and bioactivation of drugs, carcinogens, and other xenobiotics
-
-
-
additional information
?
-
-
the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens
-
-
-
additional information
?
-
-
the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate
-
-
-
additional information
?
-
P18440
the human enzyme NAT1 bioactivates arylamine and heterocyclic amine carcinogens present in red meat and tobacco products, overview
-
-
-
additional information
?
-
P50294, P50295
the isozymes catalyze the N-acetylation of aromatic amine and hydrazine drugs and carcinogens, after N-hydroxylation, they also catalyze the metabolic activation of N-hydroxy-arylamines via O-acetylation
-
-
-
additional information
?
-
-
variable expression of isozyme NAT1 due to genetic polymorphism, gene regulation or environmental influences is associated with individual susceptibility to various cancers, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
acetyl-CoA
-
-
acetyl-CoA
-
Glu60 and especially Lys136 play important roles in acetyl-CoA binding
acetyl-CoA
-
-
acetyl-CoA
Q6HVT9, Q81PT0, Q81R98
;
acetyl-CoA
-
-
acetyl-CoA
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
activation
K+
-
activation
Mg2+
-
activation
Na+
-
activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(-)-epigallocatechin-3-O-gallate
-
EGCG, non-competitive
(5Z)-3-amino-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(2-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
i.e. Rhod-o-hp. Significant reduction of cell growth by increasing the percent of MDA-MB-231 cells in G2/M phase of the cell cycle, and reduction of the ability of cells to grow in soft agar
(5Z)-5-(2-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(2-methylbenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3,4-dichlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3-hydroxy-2,4-diiodobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(3-hydroxybenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
(5Z)-5-(4-chlorobenzylidene)-2-thioxo-1,3-thiazolidin-4-one
-
inhibition of both recombinant enzyme and native enzyme in ZR-75 cell lysate, competitive
1-butoxy-2-methylbenzene
-
-
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris, the component or extract has no effect on a Nat-deficient Mycobacterium bovis strain; a drimane sesquiterpenoid lactone isolated from Warburgia salutaris. Compound inhibits the growth of Mycobacterium bovis BCG wild-type in a dose-dependent fashion, while it had little effect of the growth of Mycobacterium bovis BCG nat deleted mutant
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris, 35% inhibition of the recombinant enzyme at 0.5 mg/ml; a novel drimane sesquiterpenoid lactone isolated from Warburgia salutaris. Compound inhibits recombinant NAT activity of Mycobacterium smegmatis (35% inhibition of acetylation at 0.5 mg/mL). Compound exhibits anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium bovis BCG Pasteur. Compound does not inhibit growth of Escherichia coli and is not toxic to cultured mammalian macrophage cells at the concentrations at which anti-mycobacterial activity is observed; isolated from the bark of Warburgia salutaris
11alpha-hydroxycinnamosmolide
-
a drimane sesquiterpenoid lactone from Warburgia salutaris inhibits the purified mycobacterial enzyme and contributes to the anti-mycobacterial activity of Warburgia salutaris
17-hydroxy-beta-estradiol
-
-
17-hydroxy-progesterone
-
-
2,3-Dihydroxybiphenyl
-
the growth of strain BCG is retarded when the cultures are incubated either in the presence of 2,3-dihydroxybiphenyl on both solid and liquid media
2-bromoacetanilide
-
irreversible inhibitor
2-butoxyphenol
-
-
2-nitrosofluorene
-
; potent inactivator, incubation with 2-nitrosofluorene causes 91% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 28%
2-nitrosotoluene
-
; less potent inactivator of NAT1, NAT1 with 2-nitrosotoluene causes 46% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 5%
3',5'-ADP
-
kinetics
3-chlorocatechol
-
the growth of strain BCG is retarded when the cultures are incubated either in the presence of 3-chlorocatechol on both solid and liquid media
4-hydroxy tamoxifen
-
-
4-nitrosobenzene
-
less potent inactivator of NAT1, NAT1 with nitrosobenzene causes 59% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 13%
4-nitrosobiphenyl
-
; potent inactivator, incubation with 4-nitrosobiphenyl causes 71% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 35%
5-aminosalicylic acid
-
substrate inhibition, in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex in non-productive reaction pathways
5-aminosalicylic acid
-
substrate inhibition; substrate inhibition through 5-aminosalicylic acid in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM in Colo 205 cells, inhibitory at lower dosage of 0.05-0.5 mM, concentrations of 5-25 mM have no effect in Colo 205 cells
acetoacetyl-CoA
-
0.1 mM, 44% inhibtion
acetoaminophen
-
-
Ag+
-
; 26% remaining activity isoenzyme Nat-b; 30% remaining activity isoenzyme NAT-a
alpha-zearalenol
-
-
AMP
-
kinetics
androstenedione
-
-
Berberine
-
berberine affects kinetic constants of NAT, 24 h berberine treatment decreases bacterial growth and amounts of 2-acetylaminofluorene in Salmonella typhimurium by downregulation of the NAT enzyme expression
beta-methylesculetin
-
inhibits NAT2 but not NAT1
beta-zearalenol
-
-
bisphenol A
-
-
bromoacetamide
-
NAT2
Bromoacetanilide
-
product analogue, kinetics
Butyryl-CoA
-
0.1 mM, 28% inhibtion
Ca2+
-
at increased concentrations
caffeic acid
-
4 mM, 65.3% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 37.9% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 37.6% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 39.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
4 mM, 83.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
caffeic acid
-
non-competitive
caffeic acid
-
inhibits NAT1 but not NAT2
Chloro-beta-naphthalide
-
-
chlorogenic acid
-
dose-dependent enzyme inhibition in cytosolic and intact cell examinations
chlorogenic acid
-
4mM, significant inhibitory effect for 4 hours. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
chlorogenic acid
-
dose-dependent enzyme inhibition in cytosolic and intact cell examinations
cholate
-
-
cisplatin
-
; exposure of MCF-7 breast cancer cells to cisplatin at clinically relevant concentrations (below 0.4 nM) causes significant dose-dependent inhibition of the endogenous NAT1 enzyme. The incubation of NAT1 with various concentrations of cisplatin results in the dose-dependent modification of cysteine residues, as indicated by the disappearance of fluorescein-conjugated iodoacetamide labeling
cisplatin
-
treating C57BL/6J mice with cisplatin significantly decreases murine Nat2 (murine counterpart of human NAT1) enzymatic function in the liver (25% inhibition), kidney (40% inhibition), and blood cells (50% inhibition)
Co2+
-
; 39% remaining activity isoenzyme NAT-a
coenzyme A
-
-
coenzyme A
-
-
Cu2+
-
reversible by EDTA or histidine
Cu2+
-
; 30% remaining activity isoenzyme Nat-b
curcumin
-
non-competitive
curcumin
-
inhibits NAT2 but not NAT1
cytokine
-
mixture of proinflammatory cytokines, interferon-gamma, interleukin-1beta, tumor necrosis factor-alpha
-
deoxycholate
-
-
diethyldicarbonate
-
-
diethyldicarbonate
-
-
diethylstilbestrol
-
-
dihydrofolic acid
-
-
dithiothreitol
-
above 0.5 mM, not
dithiothreitol
-
-
DTNB
-
-
DTNB
-
partly reversible by dithioerythritol
EDTA
-
partly reversible by MgCl2
ellagic acid
-
48.9% to 80.3% inhibition of the enzyme activity in the intact cell, time- and dose-dependent inhibition, ellagic acid suppresses enzyme activity in six clinically isolated Pseudomonas aeruginosa strains
ellagic acid
-
-
esculetin
-
;
estrone
-
-
ferulic acid
-
4 mM, 46.5% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 41.4% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 35.8% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 39.7% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
4 mM, 75.1% suppression. Dose-dependent enzyme inhibition in cytosolic and intact cell examinations
ferulic acid
-
-
ferulic acid
-
inhibits NAT1 but not NAT2
folic acid
-
competitive
gallic acid
-
-
gallic acid
-
inhibits NAT1 but not NAT2
genistein
-
;
genistein
-
-
glucosamine
-
-
glucosamine 6-phosphate
-
-
glutaryl CoA
-
0.1 mM, 34% inhibtion
glycyrrhizic acid
-
-
H2O2
-
NAT1 is reversibly inactivated by physiological aoncentrations of hydrogen peroxide. Inactivation of NAT1 is fully reversed by physiological concentrations of GSH
Hexanoyl-CoA
-
0.1 mM, 24% inhibtion
Hg2+
-
; 11% remaining activity isoenzyme Nat-b; 36% remaining activity isoenzyme NAT-a
hydrogen peroxide
-
inactivates isozyme NAT1, in vivo effect, overview
hydrogen peroxide
-
inhibition is caused by oxidation at the active site cysteine
Ibuprofen
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
-
iodoacetamide
-
Cys68 is the only site of alkylation, NAT2
iodoacetamide
-
50% inhibition at 0.25 mM, 90% inhibition at 1.0 mM
iodoacetamide
-
-
iodoacetamide
-
incubation of TBNAT with iodoacetamide results in a time-dependentloss of enzymatic activity
iodoacetic acid
-
-
iodoacetic acid
-
-
iodoacetic acid
-
-
iodoacetic acid
-
; 13% remaining activity
K+
-
at increased concentrations
kaemferol
-
non-competitive; non-competitive
kaempferol
-
inhibits NAT1 and NAT2
Ketoprofen
-
competitive inhibitor of NAT enzymes
luteolin
-
-
malonyl-CoA
-
0.1 mM, 11 inhibtion
methopterin
-
kinetics
methotrexate
-
inhibits reaction with 4-aminobenzoic acid, but with sulfamethazine, possibly due to two different enzymes
methotrexate
-
competitive
Mg2+
-
at increased concentrations
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-Hydroxy-2-acetylaminofluorene
-
mechanism-based inactivator, kinetics
N-Hydroxy-2-acetylaminofluorene
-
mechanism-based inactivator, kinetics
Na+
-
at increased concentrations
nitrosobenzene
-
-
Octanoyl-CoA
-
0.1 mM, 74% inhibtion
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-mercuribenzoate
-
-
p-mercuribenzoate
-
-
paclitaxel
-
inhibits NAT1 and NAT2
palmitoyl-CoA
-
-
peroxinitrite
-
rapid and irreversible inactivation
peroxynitrite
-
inactivates isozyme NAT1, in vivo effect, overview
peroxynitrite
-
inhibition is caused by oxidation at the active site cysteine
Phenylglyoxal
-
-
Phenylglyoxal
-
-
pregnenolone
-
-
proinflammatory cytokine
-
treatment of cholangiocarcinoma KKU-100 cells with cytokines (interferon-gamma, interleukin-1beta and tumor necrosis factor-alpha) suppresses NAT1 activity, reducing the Vmax without affecting the Km
-
propionyl-CoA
-
-
quercetin
-
non-competitive; non-competitive
quercetin
-
inhibits NAT1 and NAT2
S-nitroso-glutathione
-
treatment of cholangiocarcinoma KKU-100 cells S-nitroso-glutathione results in reduced NAT1 activity as early as 2 h, and the suppression persists for 48 h
S-nitroso-N-acetyl-DL-penicillamine
-
reversible inactivation due to direct atteck of the highly reactive cysteine residue in the enzyme active site on the sulfur of S-nitrosothiols to form a mixed disulfide between these NO-derived oxidants and NAT1
S-nitrosoglutathione
-
-
salicylic acid
-
-
scopoletin
-
-
silymarin
-
-
succinyl-CoA
-
0.1 mM, 96% inhibtion
tamoxifen
-
-
taurocholate
-
-
taxifolin
-
;
testosterone
-
-
Triton X-100
-
-
vitamin C
-
-
Warburgia salutaris extract
-
-
-
zearalenone
-
-
Zn2+
-
reversible by dimercaptosuccinate
Zn2+
-
; 39% remaining activity isoenzyme Nat-b; 41% remaining activity isoenzyme NAT-a
additional information
-
no inhibition by acetylated products and 2-mercaptoethanol
-
additional information
-
-
-
additional information
-
no inhibition by CdCl2, arsenite, diisopropylfluorophosphate
-
additional information
-
-
-
additional information
-
no inhibition by CdCl2, arsenite, diisopropylfluorophosphate; no inhibition by paraoxon
-
additional information
-
no significant inactivation with either iodoacetic acid or bromoacetic acid, NAT2
-
additional information
-
inhibitory potency of flavonoids on isozyme NAT1, no inhibition by curcumin, silymarin, and scopoletin, overview; inhibitory potency of flavonoids on isozyme NAT2, no inhibition by ()epigallocatechin gallate, gallic acid, caffeic acid, and ferulic acid, overview
-
additional information
-
inhibition profile by polyphenol compounds is different between NAT1 and NAT2. The small polyphenol of cinnamic acid derivates shows some inhibitory activity toward NAT1 , but it has very low activity toward NAT2
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
1 mM, activation
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM in Colo 205 cells, inhibitory at lower dosage of 0.05-0.5 mM in Colo 205 cells and at 0.5-0.25 mM in SC-M1 cells, concentrations of 5-25 mM have no effect in Colo 205 cells
5-methoxypsoralen
-
i.e. 5-MOP, activates the enzyme at 50 mM and the further metabolism of the product of the enzyme catalytic reaction 2-acetylaminofluorene in the stomach and colon
albumin
-
activation, with long-chain primary amines as substrate
-
cysteine
-
activation
Dimercaptosuccinate
-
1 mM, activation
Penicillamine
-
1 mM, activation
R1881
the androgen R1881 induces NAT1 activity in androgen receptor-positive prostate cancer cells
glycyrrhizic acid
-
slightly activates isozyme NAT1
additional information
-
no activation by EDTA or histidine
-
additional information
the androgen R1881 induces the enzyme about 4.5fold in androgen receptor AR-positive prostate cell lines 22Rv1 and LNCaP, but not in the AR-negative PC-3, HK-293, or HeLa cells, androgen up-regulation of NAT1 is prevented by the AR antagonist flutamide, the effect of R1881 may not be by direct transcriptional activation of NAT1, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.11
2,3-dimethylaniline
-
-
5.53
2,3-dimethylaniline
-
-
2.06
2,4-dimethylaniline
-
-
5.03
2,4-dimethylaniline
-
-
3.12
2,5-dimethylaniline
-
-
6.73
2,5-dimethylaniline
-
-
0.81
2-amino-4-chlorophenol
pH 7.0, 30C, recombinant enzyme
1.9
2-amino-4-chlorophenol
-
-
1.9
2-amino-4-methylphenol
pH 7.0, 30C, recombinant enzyme
3.06
2-amino-4-methylphenol
-
-
13
2-aminobenzoic acid
-
-
0.000153
2-Aminofluorene
-
-
0.00019
2-Aminofluorene
-
-
0.00055
2-Aminofluorene
-
-
0.033
2-Aminofluorene
recombinant isozyme Nat3
0.037
2-Aminofluorene
recombinant isozyme Nat1
0.044
2-Aminofluorene
-
pH 7.4, 37C
0.064
2-Aminofluorene
-
pH 7.4, 37C
0.079
2-Aminofluorene
-
pH 7.4, 37C
0.082
2-Aminofluorene
-
pH 7.4, 37C
0.084
2-Aminofluorene
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
0.096
2-Aminofluorene
-
pH 7.4, 37C
0.106
2-Aminofluorene
-
pH 7.4, 37C
0.109
2-Aminofluorene
-
-
0.117
2-Aminofluorene
-
pH 7.4, 37C
0.127
2-Aminofluorene
-
strain 54.8, pH 7.4, 37C
0.141
2-Aminofluorene
-
pH 7.4, 37C
0.158
2-Aminofluorene
-
pH 7.4, 37C
0.165
2-Aminofluorene
-
pH 7.4, 37C
0.17
2-Aminofluorene
-
-
0.205
2-Aminofluorene
-
strain K-12, pH 7.4, 37C
0.21
2-Aminofluorene
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.238
2-Aminofluorene
-
wild-type enzyme
0.286
2-Aminofluorene
-
-
0.298
2-Aminofluorene
-
mutant enzyme I238T
0.301
2-Aminofluorene
-
mutant enzyme 245I
0.304
2-Aminofluorene
-
mutant enzyme W77R
0.311
2-Aminofluorene
-
strain 60.62, pH 7.4, 37C
0.365
2-Aminofluorene
-
strain YG1024, pH 7.4, 37C
0.367
2-Aminofluorene
-
mutant enzyme K185N
0.397
2-Aminofluorene
-
strain TA98, pH 7.4, 37C
0.404
2-Aminofluorene
-
strain TA1538, pH 7.4, 37C
0.504
2-Aminofluorene
-
-
0.568
2-Aminofluorene
recombinant isozyme Nat2
0.72
2-Aminofluorene
-
-
0.81
2-Aminofluorene
-
-
0.842
2-Aminofluorene
-
-
0.857
2-Aminofluorene
-
mutant enzyme N172I
1.06
2-Aminofluorene
-
-
1.1
2-Aminofluorene
-
-
2.92
2-Aminofluorene
-
-
1.4
2-Aminophenol
pH 7.0, 30C, recombinant enzyme
5.79
2-Aminophenol
-
-
2.11
2-ethylaniline
-
-
2.32
2-methylaniline
-
-
7.18
2-methylaniline
-
-
1.1
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
1.2
2-toluidine
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
6.6
2-toluidine
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.352
3,4-dimethylaniline
-
-
0.688
3,4-dimethylaniline
-
-
0.5
3,5-dimethyl-4-nitroaniline
-
-
0.28
3,5-dimethylaniline
-
-
0.742
3,5-dimethylaniline
-
-
2.6
3-amino-4-hydroxybenzaldehyde
pH 7.0, 30C, recombinant enzyme
12
3-amino-4-hydroxybenzenesulfonic acid
pH 7.0, 30C, recombinant enzyme
0.32
3-Amino-4-hydroxybenzoic acid
-
-
2
3-Amino-4-hydroxybenzoic acid
pH 7.0, 30C, recombinant enzyme
12.5
3-aminobenzoic acid
-
-
0.576
3-ethylaniline
-
-
1.32
3-ethylaniline
-
-
0.9
4-amino-3-hydroxybenzoic acid
-
-
0.17
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
0.18
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
11
4-amino-3-methylbenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.04
4-amino-4'-nitroazobenzene
-
-
2.42
4-Aminobenzoate
-
-
0.049
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
0.061
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.23
4-Aminobenzoic acid
pH 7.0, 25C, recombinant wild-type enzyme; pH 7.0, 25C, recombinant wild-type enzyme
0.37
4-Aminobenzoic acid
pH 7.0, 25C, recombinant mutant R64W; pH 7.0, 25C, recombinant mutant R64W
2.25
4-Aminobenzoic acid
-
-
2.34
4-Aminobenzoic acid
-
-
166
4-Aminobenzoic acid
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
0.0058
4-aminobiphenyl
recombinant isozyme Nat1
0.018
4-aminobiphenyl
recombinant isozyme Nat3
0.191
4-aminobiphenyl
-
-
0.486
4-aminobiphenyl
-
-
1.57
4-aminobiphenyl
recombinant isozyme Nat2
0.834
4-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.258
4-aminosalicylic acid
-
pH 7.4, 37C
0.86
4-aminosalicylic acid
-
pH 7.4, 37C
1.16
4-aminosalicylic acid
-
strain K-12, pH 7.4, 37C
1.24
4-aminosalicylic acid
-
strain 54.8, pH 7.4, 37C
1.79
4-aminosalicylic acid
-
pH 7.4, 37C
2.2
4-aminosalicylic acid
-
pH 7.4, 37C
1.8
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
2.3
4-anisidine
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
3.7
4-anisidine
-
wild-type, pH 8.0, 28C
1.8
4-bromoaniline
-
0.33
4-butoxyaniline
-
2
4-chloroaniline
-
6
4-chloroaniline
-
-
1.01
4-ethoxyaniline
-
0.205
4-ethylaniline
-
-
3.27
4-ethylaniline
-
-
51
4-fluoroaniline
-
-
20
4-hydroxybenzhydrazide
-
-
0.483
4-methylaniline
-
-
11
4-methylaniline
-
-
118
4-methylaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.28 - 0.37
4-nitroaniline
-
-
0.3
4-nitrobenzalhydrazone
-
-
2.8
4-nitrophenyl acetate
-
mutant Y190A, pH 7.0, 25C
3.5
4-nitrophenyl acetate
-
mutant Y190F, pH 7.0, 25C
4.1
4-nitrophenyl acetate
-
mutant Y190I, pH 7.0, 25C
4.81
4-nitrophenyl acetate
pH 7.0, 25C, recombinant mutant R64W; pH 7.0, 25C, recombinant mutant R64W
9
4-nitrophenyl acetate
-
wild-type, pH 7.0, 25C
10
4-nitrophenyl acetate
pH 7.0, 25C, recombinant wild-type enzyme; pH 7.0, 25C, recombinant wild-type enzyme
3.2
4-phenylenediamine
-
-
5.4
4-phenylenediamine
-
-
0.2
5-Aminosalicylate
-
-
0.228
5-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.25
5-Aminosalicylate
-
0.622
5-Aminosalicylate
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
0.0067
5-aminosalicylic acid
isoform NAT 1, pH 7.4, 37C
0.026
5-aminosalicylic acid
-
pH 7.4, 37C
0.061
5-aminosalicylic acid
-
pH 7.4, 37C
0.112
5-aminosalicylic acid
-
pH 7.4, 37C
0.117
5-aminosalicylic acid
-
strain 54.8, pH 7.4, 37C
0.158
5-aminosalicylic acid
-
pH 7.4, 37C
0.184
5-aminosalicylic acid
isoform NAT 2, pH 7.4, 37C
0.208
5-aminosalicylic acid
-
strain 60.62, pH 7.4, 37C
0.21
5-aminosalicylic acid
-
pH 7.4, 37C
0.219
5-aminosalicylic acid
-
-
0.347
5-aminosalicylic acid
-
pH 7.4, 37C
1
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
1.02
5-aminosalicylic acid
-
-
1.2
5-aminosalicylic acid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
1.7
5-aminosalicylic acid
-
wild-type, pH 8.0, 28C
0.0084
acetyl-CoA
-
-
0.013
acetyl-CoA
-
-
0.02
acetyl-CoA
-
-
0.028
acetyl-CoA
-
-
0.05
acetyl-CoA
-
-
0.07
acetyl-CoA
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
0.11 - 0.125
acetyl-CoA
-
-
0.136
acetyl-CoA
-
concentration p-anisidine: 200 nM, reaction: acetyl-CoA + p-anisidine
0.158
acetyl-CoA
-
wild-type, pH 8.0, 28C
0.181
acetyl-CoA
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
0.2
acetyl-CoA
-
using 5-aminosalicylate as a substrate
0.466
acetyl-CoA
-
concentration p-anisidine: 2 mM, reaction: acetyl-CoA + p-anisidine
0.77
acetyl-CoA
-
-
1.02
acetyl-CoA
-
-
560
acetyl-CoA
-
wild-type, pH 8.0, 28C
2.49
aniline
-
-
112
aniline
-
-
14
benzoic acid hydrazide
-
-
0.77 - 0.8
decylamine
-
-
0.77 - 0.8
decylamine
-
-
0.3
dodecylamine
-
-
0.14
Hexylamine
-
-
0.72
histamine
-
-
0.61
hydralazine
-
-
0.849
hydralazine
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatB
1.327
hydralazine
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.71 - 0.72
hydroxytyramine
-
-
0.18
Isoniazid
-
-
0.366
Isoniazid
-
in vitro result for allele NAT2*7
0.374
Isoniazid
-
in vitro result for allele NAT2*4
1.414
Isoniazid
; apparent KM
4.7
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
6.1
Isoniazid
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
6.2
Isoniazid
-
wild-type, pH 8.0, 28C
0.38 - 0.58
isoniazide
-
-
3.2
N,N-1,4-phenylenediamine
-
Vmax: 0.17 micromol/min/mg
5.4
N,N-1,4-phenylenediamine
-
Vmax: 2.1 micromol/min/mg
0.058
N-Hydroxy-4-aminobiphenyl
recombinant isozyme Nat1
0.062
N-Hydroxy-4-aminobiphenyl
recombinant isozyme Nat3
3.3
N-Hydroxy-4-aminobiphenyl
recombinant isozyme Nat2
14
nicotinic acid hydrazide
-
-
0.08
octylamine
-
-
0.08
octylamine
-
-
0.0028
p-Aminobenzoic acid
-
control; using control cholangiocarcinoma KKU-100 cells without cytokine administration, Vmax: 211.2 pmol/min/mg
0.0033
p-Aminobenzoic acid
-
cytokine mixture; using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount of enzyme but not in its affinity
0.01
p-Aminobenzoic acid
-
at fixed concentration of 0.1 mM acetyl-CoA
0.084
p-Aminobenzoic acid
-
-
0.283
p-Aminobenzoic acid
-
strain 60.62, pH 7.4, 37C
0.87
p-Aminobenzoic acid
-
pH 7.4, 37C
1.2
p-Aminobenzoic acid
-
pH 7.4, 37C
2.9
p-Aminobenzoic acid
-
at fixed concentration of 0.1 mM acetyl-CoA
5
p-Aminobenzoic acid
-
-
51.6
p-Aminobenzoic acid
-
strain YG1024, pH 7.4, 37C
152
p-Aminobenzoic acid
-
-
0.059
p-Aminosalicylic acid
-
-
0.184
p-Aminosalicylic acid
-
mutant G129A
0.216
p-Aminosalicylic acid
-
-
0.447
p-Aminosalicylic acid
-
mutant G129S
5.39
p-Aminosalicylic acid
-
-
0.136
p-anisidine
-
pH8, 37C
1.3
p-octopamine
-
liver
0.14 - 0.145
p-tyramine
-
-
0.14 - 0.145
p-tyramine
-
-
0.184
PAS
-
mutant G129A
0.216
PAS
-
wild-type
0.447
PAS
-
mutant G129S
0.18
Phenylethylamine
-
-
0.3
Phenylethylamine
-
-
45
procainamide
-
isozyme NAT1
0.11 - 0.125
serotonin
-
brain
0.25
serotonin
-
-
0.42
serotonin
-
-
3.1
Sulfadiazine
-
-
0.02
sulfamethazine
-
mutant F125S
0.059
sulfamethazine
-
strain 54.8, pH 7.4, 37C
0.07
sulfamethazine
-
pH 7.4, 37C
0.115
sulfamethazine
-
pH 7.4, 37C
0.12
sulfamethazine
-
at fixed concentration of 0.1 mM acetyl-CoA
0.121
sulfamethazine
-
strain K-12, pH 7.4, 37C
0.146
sulfamethazine
-
pH 7.4, 37C
0.55
sulfamethazine
-
pH 7.4, 37C
1.2
sulfamethazine
-
at fixed concentration of 0.1 mM acetyl-CoA
3.5
sulfamethazine
-
isozyme NAT1
5.39
sulfamethazine
-
-
0.662
sulfamethoxazole
Q6HVT9, Q81PT0, Q81R98
apparent KM; isoenzyme BanatC
0.07
tetradecylamine
-
-
0.031
tryptamine
-
-
0.24 - 0.25
tryptamine
-
-
0.24 - 0.25
tryptamine
-
liver
0.53
tryptamine
-
pineal gland
0.0088
tyramine
-
-
0.08
m-tyramine
-
-
additional information
additional information
-
reaction kinetics
-
additional information
additional information
-
kinetic data of various acetyl donors with 4-nitroaniline or aniline as acceptor amine and of various acceptor amines with acetyl-CoA as donor
-
additional information
additional information
-
assay based on HPLC
-
additional information
additional information
-
kinetic data of isozymes NAT1 and 2
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
not detectable: p-aminobenzoic acid
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
not detectable: 4-aminosalicylic-acid, pH 7.4, 37C
-
additional information
additional information
-
not detectable: 5-aminosalicylic acid
-
additional information
additional information
-
steady-state kinetics, kinetic and isotope effect studies
-
additional information
additional information
-
kinetics analysis, kinetic mechanism, determination of the half-life of the acetylated enzyme intermediate as 270 s, overview
-
additional information
additional information
-
kinetics; kinetics
-
additional information
additional information
-
NAT1 shows much higher affinity for p-amino benzoic acid than for sulfamethazine while NAT2 shows the opposite selectivity
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
27
2,3-dimethylaniline
Homo sapiens
-
-
256
2,3-dimethylaniline
Homo sapiens
-
-
67
2,4-dimethylaniline
Homo sapiens
-
-
661
2,4-dimethylaniline
Homo sapiens
-
-
5.7
2,5-dimethylaniline
Homo sapiens
-
-
313
2,5-dimethylaniline
Homo sapiens
-
-
0.27
2-amino-4-chlorophenol
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
21
2-amino-4-chlorophenol
Mycobacterium tuberculosis
-
-
1.3
2-amino-4-methylphenol
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
70
2-amino-4-methylphenol
Mycobacterium tuberculosis
-
-
449
2-Aminofluorene
Homo sapiens
-
-
759
2-Aminofluorene
Homo sapiens
-
-
0.41
2-Aminophenol
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
51
2-Aminophenol
Mycobacterium tuberculosis
-
-
61
2-ethylaniline
Homo sapiens
-
-
12
2-methylaniline
Homo sapiens
-
-
111
2-methylaniline
Homo sapiens
-
-
4.8
2-toluidine
Mesocricetus auratus
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
6.3
2-toluidine
Homo sapiens
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
72
2-toluidine
Homo sapiens
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
461
3,4-dimethylaniline
Homo sapiens
-
-
800
3,4-dimethylaniline
Homo sapiens
-
-
308
3,5-dimethylaniline
Homo sapiens
-
-
1220
3,5-dimethylaniline
Homo sapiens
-
-
0.29
3-amino-4-hydroxybenzaldehyde
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
1.1
3-amino-4-hydroxybenzenesulfonic acid
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
1.3
3-Amino-4-hydroxybenzoic acid
Streptomyces griseus
A2V7M5
pH 7.0, 30C, recombinant enzyme
94
3-Amino-4-hydroxybenzoic acid
Mycobacterium tuberculosis
-
-
310
3-ethylaniline
Homo sapiens
-
-
1960
3-ethylaniline
Homo sapiens
-
-
66
4-amino-3-hydroxybenzoic acid
Mycobacterium tuberculosis
-
-
1.3
4-amino-3-methylbenzoic acid
Homo sapiens
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
14.2
4-amino-3-methylbenzoic acid
Mesocricetus auratus
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
16.6
4-amino-3-methylbenzoic acid
Homo sapiens
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
8
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate acetyl-CoA, mutant Y190A, pH 7.0, 25C
21
4-Aminobenzoic acid
Homo sapiens
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
38
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate 4-nitrophenyl acetate, mutant Y190A, pH 7.0, 25C
58
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate acetyl-CoA, mutant Y190I, pH 7.0, 25C
60
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate 4-nitrophenyl acetate, mutant Y190I, pH 7.0, 25C
127
4-Aminobenzoic acid
Homo sapiens
-
pH 7.0, 23C, isozyme NAT1, with 4-nitrophenyl acetate
189
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate acetyl-CoA, mutant Y190F, pH 7.0, 25C
200
4-Aminobenzoic acid
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
200
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate acetyl-CoA, wild-type, pH 7.0, 25C
230
4-Aminobenzoic acid
Mesocricetus auratus
-
pH 7.0, 23C, isozyme NAT2, with 4-nitrophenyl acetate
265
4-Aminobenzoic acid
Mesocricetus auratus
P50292
pH 7.0, 25C, recombinant mutant R64W, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant mutant R64W, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant wild-type enzyme, with 4-nitrophenyl acetate; pH 7.0, 25C, recombinant wild-type enzyme, with 4-nitrophenyl acetate
393
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate 4-nitrophenyl acetate, mutant Y190F, pH 7.0, 25C
620
4-Aminobenzoic acid
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
620
4-Aminobenzoic acid
Mesocricetus auratus
-
cosubstrate 4-nitrophenyl acetate, wild-type, pH 7.0, 25C
16
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
mutant Y190A, pH 7.0, 25C
76
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
77
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
mutant Y190I, pH 7.0, 25C
86
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
mutant Y190F, pH 7.0, 25C
120
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
120
4-aminobenzoyl glutamic acid
Mesocricetus auratus
-
wild-type, pH 7.0, 25C
243
4-aminobiphenyl
Homo sapiens
-
-
256
4-aminobiphenyl
Homo sapiens
-
-
0.03
4-anisidine
Mycobacterium smegmatis
O86309
-
0.3
4-anisidine
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
0.6
4-anisidine
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5.2
4-anisidine
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
0.0086
4-bromoaniline
Mycobacterium smegmatis
O86309
-
0.039
4-butoxyaniline
Mycobacterium smegmatis
O86309
-
0.0069
4-chloroaniline
Mycobacterium smegmatis
O86309
-
0.2
4-chloroaniline
Mycobacterium tuberculosis
-
-
0.036
4-ethoxyaniline
Mycobacterium smegmatis
O86309
-
25
4-ethylaniline
Oryctolagus cuniculus
-
-
430
4-ethylaniline
Homo sapiens
-
-
700
4-ethylaniline
Homo sapiens
-
-
0.9
4-fluoroaniline
Mycobacterium tuberculosis
-
-
10.9
4-hydroxybenzhydrazide
Mycobacterium tuberculosis
-
-
0.0098
4-iodoaniline
Mycobacterium smegmatis
O86309
-
0.8
4-methylaniline
Mycobacterium tuberculosis
-
-
303
4-methylaniline
Homo sapiens
-
-
1600
4-methylaniline
Homo sapiens
-
-
0.25
4-nitroaniline
Mesocricetus auratus
-
mutant Y190A, pH 7.0, 25C
0.31
4-nitroaniline
Mesocricetus auratus
-
mutant Y190F, pH 7.0, 25C
0.6
4-nitroaniline
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor acetyl-CoA, recombinant wild-type enzyme
0.6
4-nitroaniline
Mesocricetus auratus
-
wild-type, pH 7.0, 25C
0.89
4-nitroaniline
Mesocricetus auratus
-
mutant Y190I, pH 7.0, 25C
15
4-nitrophenyl acetate
Mesocricetus auratus
-
mutant Y190A, pH 7.0, 25C
57
4-nitrophenyl acetate
Mesocricetus auratus
-
mutant Y190I, pH 7.0, 25C
279
4-nitrophenyl acetate
Mesocricetus auratus
-
mutant Y190F, pH 7.0, 25C
620
4-nitrophenyl acetate
Mesocricetus auratus
P50292
pH 7.0, 25C, recombinant mutant R64W, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant mutant R64W, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant wild-type enzyme, with 4-aminobenzoic acid; pH 7.0, 25C, recombinant wild-type enzyme, with 4-aminobenzoic acid
1301
4-nitrophenyl acetate
Mesocricetus auratus
-
wild-type, pH 7.0, 25C
0.044
5-Aminosalicylate
Mycobacterium smegmatis
O86309
-
0.0017
5-aminosalicylic acid
Mycobacterium tuberculosis
-
wild-type, pH 8.0, 28C
1.8
5-aminosalicylic acid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5.2
5-aminosalicylic acid
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
434
5-aminosalicylic acid
Pseudomonas aeruginosa
-
-
0.28
aniline
Mycobacterium tuberculosis
-
-
281
aniline
Homo sapiens
-
-
711
aniline
Homo sapiens
-
-
2 - 8
anisidine
Mesocricetus auratus
-
mutant Y190A, pH 7.0, 25C
2.9
anisidine
Mycobacterium tuberculosis
-
-
85
anisidine
Mesocricetus auratus
-
mutant Y190I, pH 7.0, 25C
260
anisidine
Mesocricetus auratus
-
pH 7.0, 25C, with cofactor 4-nitrophenyl acetate, recombinant wild-type enzyme
260
anisidine
Mesocricetus auratus
-
wild-type, pH 7.0, 25C
288
anisidine
Mesocricetus auratus
-
mutant Y190F, pH 7.0, 25C
1.9
benzoic acid hydrazide
Mycobacterium tuberculosis
-
-
0.063
hydralazine
Mycobacterium smegmatis
O86309
-
44
hydralazine
Mycobacterium tuberculosis
-
-
0.1
Isoniazid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
0.3
Isoniazid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
1.7
Isoniazid
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
4.9
Isoniazid
Mycobacterium tuberculosis
-
-
0.6
nicotinic acid hydrazide
Mycobacterium tuberculosis
-
-
0.7
p-Aminobenzoic acid
Mycobacterium tuberculosis
-
-
38
p-Aminobenzoic acid
Homo sapiens
-
-
298
p-Aminobenzoic acid
Homo sapiens
-
-
27
p-Aminosalicylic acid
Homo sapiens
-
-
591
p-Aminosalicylic acid
Homo sapiens
-
-
387
sulfamethazine
Homo sapiens
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.166
4-anisidine
Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
3894
0.26
4-anisidine
Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
3894
0.018
5-aminosalicylic acid
Mycobacterium tuberculosis
-
wild-type, pH 8.0, 28C
1817
1.5
5-aminosalicylic acid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C; chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
1817
3.058
5-aminosalicylic acid
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
1817
0.166
anisidine
Mycobacterium marinum
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
5655
0.26
anisidine
Mycobacterium marinum
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
5655
1.405
anisidine
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
5655
0.009
Isoniazid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28C
851
0.121
Isoniazid
Mycobacterium marinum, Mycobacterium tuberculosis
-
chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28C
851
0.274
Isoniazid
Mycobacterium marinum
-
wild-type, pH 8.0, 28C
851
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.54
4-nitrosobenzene
-
-
0.00067
4-nitrosobiphenyl
-
; recombinant NAT1
0.54
nitrosobenzene
-
recombinant NAT1
0.01
quercetin
-
isozyme NAT2
0.0486
quercetin
-
isozyme NAT1
0.67
Bromoacetanilide
-
-