Information on EC 2.3.1.38 - [acyl-carrier-protein] S-acetyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.3.1.38
-
RECOMMENDED NAME
GeneOntology No.
[acyl-carrier-protein] S-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein]
show the reaction diagram
Bi Bi ping pong mechanism, acetyl-enzyme intermediate
-
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein]
show the reaction diagram
Arg-606 is responsible for malonyl-CoA specificity of the bifunctional enzyme; mechanism
-
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
fatty acid biosynthesis initiation II
-
superpathway of fatty acid biosynthesis initiation (E. coli)
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:[acyl-carrier protein] S-acetyltransferase
This enzyme, along with EC 2.3.1.39, [acyl-carrier-protein] S-malonyltransferase, is essential for the initiation of fatty-acid biosynthesis in bacteria. The substrate acetyl-CoA protects the enzyme against inhibition by N-ethylmaleimide or iodoacetamide [4]. This is one of the activities associated with beta-ketoacyl-ACP synthase III (EC 2.3.1.180) [5].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
ACAT
Persea sp.
-
-
acetyl coenzyme A-acyl-carrier-protein transacylase
-
-
-
-
acetyl coenzyme A:ACP transacylase
-
-
acetyl coenzyme A:ACP transacylase
Euglena gracilis Z
-
-
-
acetyl-CoA-ACP-transacylase
-
-
acetyl-CoA:ACP transacylase
Persea sp.
-
-
acetyl-CoA:acyl carrier protein transacylase
-
-
acetyltransferase, [acyl-carrier-protein]
-
-
-
-
acyl-CoA:ACP transacylase
-
-
malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
-
[ACP] acetyltransferase
-
-
-
-
[acyl-carrier-protein]acetyltransferase
-
-
-
-
malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
i.e. MAT
additional information
Persea sp.
-
cf. EC 2.3.1.85 and EC 2.3.1.86; possibility that beta-ketoacyl-[acyl-carrier-protein] synthetase III has intrinsic acetyl-CoA:[acyl-carrier-protein] transacylase activity and the separated acetyl-CoA:[acyl-carrier-protein] transacylase would be a proteolytic fragment of beta-ketoacyl-[acyl-carrier-protein] synthetase III
additional information
-
cf. EC 2.3.1.85 and EC 2.3.1.86; dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
additional information
-
cf. EC 2.3.1.85 and EC 2.3.1.86; enzyme has both a acetyl-CoA:acyl carrier protein transacylase activity and a much higher thiolase activity
CAS REGISTRY NUMBER
COMMENTARY
37257-16-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain E26
-
-
Manually annotated by BRENDA team
Escherichia coli E26
strain E26
-
-
Manually annotated by BRENDA team
v. bacillaris
-
-
Manually annotated by BRENDA team
Euglena gracilis Z
Z
-
-
Manually annotated by BRENDA team
Persea sp.
avocado
-
-
Manually annotated by BRENDA team
var. Viroflay
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + pantetheine
CoA + acetyl-S-pantetheine
show the reaction diagram
-
dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
r
acetyl-CoA + pantetheine
CoA + acetyl-S-pantetheine
show the reaction diagram
-
dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
r
acetyl-CoA + pantetheine
CoA + acetyl-S-pantetheine
show the reaction diagram
-
octanoyl-S-pantetheine, decanoyl-S-pantetheine, and dodecanoyl-S-pantetheine can also serve as substrates for the backward reaction
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
Persea sp.
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
equilibrium in forward direction
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
dual specificity of a malonyl-CoA/acetyl-CoA:acyl carrier protein S-acyltransferase
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
via acetyl-enzyme intermediate
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
via acetyl-enzyme intermediate
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
via acetyl-enzyme intermediate
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
pantetheine, not 2-mercaptoethanol can replace [acyl-carrier-protein]
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
acetyl-S-pantetheine can replace acetyl-CoA
-
-
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
acetyl-S-pantetheine can replace acetyl-CoA
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
specific for acetyl-CoA, utilizes [acyl-carrier-protein] of diverse origins with differing affinities
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
enzyme is component of fatty acid synthase complex II
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
enzyme is component of fatty acid synthase complex II
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
first step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
rate-limiting step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
rate-limiting step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
Escherichia coli E26
-
via acetyl-enzyme intermediate
-
r
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
Euglena gracilis Z
-
-, enzyme is component of fatty acid synthase complex II
-
?
butyryl-CoA + [acyl-carrier protein]
CoA + butyryl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
butyryl-CoA + [acyl-carrier protein]
CoA + butyryl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
-
butyryl-CoA + [acyl-carrier protein]
CoA + butyryl-[acyl-carrier protein]
show the reaction diagram
-
35% activity of that with acetyl-CoA
-
-
?
butyryl-CoA + [acyl-carrier protein]
CoA + butyryl-[acyl-carrier protein]
show the reaction diagram
-
9.8% activity of that with acetyl-CoA
-
-
?
butyryl-CoA + [acyl-carrier protein]
CoA + butyryl-[acyl-carrier protein]
show the reaction diagram
Escherichia coli E26
-
9.8% activity of that with acetyl-CoA
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
CoA + hexanoyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
CoA + hexanoyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
-
hexanoyl-CoA + [acyl-carrier protein]
CoA + hexanoyl-[acyl-carrier protein]
show the reaction diagram
-
4.6% activity of that with acetyl-CoA
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
CoA + hexanoyl-[acyl-carrier protein]
show the reaction diagram
-
18% activity of that with acetyl-CoA
-
-
?
octanoyl-CoA + [acyl-carrier protein]
CoA + octanoyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
octanoyl-CoA + [acyl-carrier protein]
CoA + octanoyl-[acyl-carrier protein]
show the reaction diagram
-
poor substrate, 7% activity of that with acetyl-CoA
-
-
?
octanoyl-CoA + [acyl-carrier protein]
CoA + octanoyl-[acyl-carrier protein]
show the reaction diagram
Escherichia coli, Escherichia coli E26
-
10.3% activity of that with acetyl-CoA
-
-
?
propionyl-CoA + [acyl-carrier protein]
CoA + propionyl-[acyl-carrier protein]
show the reaction diagram
-
transacylation at 23.4% the rate of acetyl-CoA
-
-
?
propionyl-CoA + [acyl-carrier protein]
CoA + propionyl-[acyl-carrier protein]
show the reaction diagram
Escherichia coli E26
-
transacylation at 23.4% the rate of acetyl-CoA
-
-
?
hexanoyl-CoA + [acyl-carrier protein]
CoA + hexanoyl-[acyl-carrier protein]
show the reaction diagram
Escherichia coli E26
-
4.6% activity of that with acetyl-CoA
-
-
?
additional information
?
-
-
2-mercaptoethanol does not substitute for [acyl-carrier-protein]
-
-
-
additional information
?
-
-
no substrate: malonyl-CoA
-
-
-
additional information
?
-
-
no substrate: malonyl-CoA
-
-
-
additional information
?
-
Persea sp.
-
no substrate: malonyl-CoA
-
-
-
additional information
?
-
-
in vertebrates, yeast and Mycobacteria acetyl transferase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86
-
-
-
additional information
?
-
-
enzyme is part of fatty acid synthetase complex, consisting of 2 subunits
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
enzyme is component of fatty acid synthase complex II
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
enzyme is component of fatty acid synthase complex II
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
first step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
part of non-associated fatty acid synthase system of plants and prokaryotes
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
rate-limiting step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
-
rate-limiting step of fatty acid synthesis
-
?
acetyl-CoA + [acyl-carrier protein]
CoA + acetyl-[acyl-carrier protein]
show the reaction diagram
Euglena gracilis Z
-
enzyme is component of fatty acid synthase complex II
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
high ionic strength required for maximal activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
acetyl-CoA
-
at high concentration
acyl-carrier protein
-
at high concentration
cerulenin
Persea sp.
-
antibiotic, weak inhibition
CoA
-
competitive
iodoacetamide
-
-
iodoacetamide
-
acetyl-CoA protects
iodoacetamide
-
acetyl-CoA protects; strong
iodoacetamide
-
no inhibition
iodoacetamide
-
acetyl-CoA protects; strong
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-ethylmaleimide
-
acetyl-CoA protects
N-ethylmaleimide
-
acetyl-CoA protects; strong
N-ethylmaleimide
-
weak
p-chloromercuribenzoate
-
strong inhibition
p-Substituted mercuribenzoate
-
-
pantetheine
-
weak, competitive
thiolactomycin
Persea sp.
-
-
methyl methanethiosulfonate
-
weak
additional information
-
no inhibition by PMSF
-
additional information
-
no inhibition by diisopropylphosphofluoridate and antibiotic cerulenin
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0018
-
acetyl-CoA
-
recombinant purified mutant R606A
0.0029
-
acetyl-CoA
-
recombinant purified mutant R606K
0.0039
-
acetyl-CoA
-
recombinant purified wild-type enzyme
0.0043
-
acetyl-CoA
-
native wild-type enzyme
0.008
-
acetyl-CoA
-
-
0.0087
-
acetyl-CoA
-
recombinant wild-type enzyme
0.05
-
acetyl-CoA
-
-
0.035
-
acyl-carrier protein
-
-
0.0083
-
Butyryl-CoA
-
-
0.0086
-
Hexanoyl-CoA
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0225
-
acetyl-CoA
-
recombinant wild-type, crude Streptomyces glaucescens [acyl-carrier-protein]
0.0508
-
acetyl-CoA
-
native wild-type, crude E. coli [acyl-carrier-protein]
1.9
-
acetyl-CoA
-
recombinant purified wild-type enzyme
3.4
-
acetyl-CoA
-
recombinant purified mutant R606K
4.2
-
acetyl-CoA
-
recombinant purified mutant R606A
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.2
-
pantetheine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000005
-
-
-
0.000009
-
-
crude enzyme extract
0.000018
-
-
-
0.000064
-
-
-
0.00019
-
-
-
0.00023
-
-
crude extract
0.0056
-
-
purified enzyme
0.0056
-
-
partially purified enzyme
0.022
-
-
purified native wild-type enzyme
0.03
-
-
partially purified enzyme
0.059
-
-
-
0.3
-
-
purified enzyme
0.8
-
-
approx., purified enzyme
2.68
-
-
recombinant purified wild-type enzyme
3.22
-
-
recombinant purified wild-type enzyme, carboxyterminally FLAG-tagged
3.81
-
-
recombinant purified wild-type enzyme, carboxyterminally His-tagged
4.53
-
-
recombinant purified mutant R606K
17.8
-
-
recombinant purified mutant R606A
additional information
-
-
fatty acid synthase II assay
additional information
-
-
fatty acid synthase II assay
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
assay at
6.8
-
-
assay at
7
-
Persea sp.
-
-
8
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8.5
-
about 60% of maximal activity at pH 5.0 and pH 8.5
6.2
7.5
Persea sp.
-
about half-maximal activity at pH 6.0 and pH 7.5
7.5
9.1
-
about half-maximal activity at pH 7.5 and about 75% of maximal activity at pH 9.1
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
25
-
-
assay at
30
-
-
assay at
30
-
-
assay at
35
-
-
assay at
38
-
-
assay at
38
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Persea sp.
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
fatty acid synthase II
Manually annotated by BRENDA team
Persea sp.
-
-
Manually annotated by BRENDA team
-
fatty acid synthase II
Manually annotated by BRENDA team
Persea sp.
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
18500
-
Persea sp.
-
gel filtration
48000
-
-
gel filtration
60000
-
-
gel filtration
61000
-
-
gel filtration
147000
-
-
gel filtration
185000
-
-
gel filtration
additional information
-
-
amino acid sequence, comparison
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
-
4 * 45000, SDS-PAGE
dimer
-
2 * 29000, SDS-PAGE
additional information
-
vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-
30% loss of activity after 40 min
50
-
-
t1/2: 18 min
60
-
-
t1/2: 9 min
100
-
-
inactivation after 1 min
additional information
-
-
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
EDTA at 5 mM stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10C, unpredictable instability on storage, sometimes total loss of activity occured immediately after thawing
-
-20C, at least 1 month
-
-20C, less than 10% of activity retained after 1 year
-
-20C, several months
-
0C, inactivation within several days
-
-70C, several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
Persea sp.
-
recombinant mutants from E. coli
-
native wild-type and recombinant wild-type and mutant from E. coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expression of wild-type and His-tagged mutant S581A as His-tagged proteins in Escherichia coli and as FLAG-tagged proteins in Sf9 insect cells via baculovirus infection
-
expression of wild-type and mutants in Escherichia coli BL21 (DE3)
-
cloning and sequencing of fadA gene, expression in Escherichia coli of wild-type and C92S mutant
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C159A
-
no activity
C59S
-
no activity
R606A
-
oligonucleotide-directed in vitro mutagenesis, 6.6fold increase in activity about 16000fold increase in selectivity for acetyl-CoA against malonyl-CoA, increased production of medium-chain length fatty acids
R606K
-
oligonucleotide-directed in vitro mutagenesis, 1.7fold increase in activity about 16fold increase in selectivity for acetyl-CoA against malonyl-CoA
S581A
-
nearly no remaining activity, below 0.03% compared to wild-type
C92S
-
no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant enzyme purified from E. coli, refolding after treatment of inclusion bodies with urea
-