Information on EC 2.3.1.190 - acetoin dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.3.1.190
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RECOMMENDED NAME
GeneOntology No.
acetoin dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetoin + CoA + NAD+ = acetaldehyde + acetyl-CoA + NADH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acetoin degradation
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:acetoin O-acetyltransferase
Requires thiamine diphosphate. This enzyme, which belongs to the family of 2-oxo acid dehydrogenase complexes, catalyses the oxidative-hydrolytic cleavage of acetoin to acetaldehyde and acetyl-CoA in many bacterial strains, both aerobic and anaerobic. The enzyme is composed of multiple copies of three enzymic components: acetoin oxidoreductase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoyl dehydrogenase (E3).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Alcaligenes eutrophus H16
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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disruption of the gene acoA encoding subunit E1alpha results in strains impaired in expression of dephosphate-dependent E1 activity, to remove acetoin from the medium and to grow with acetoin as sole carbon source
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetoin + 2,6-dichlorophenolindophenol
acetaldehyde + ?
show the reaction diagram
diacetyl + 2,6-dichlorophenolindophenol
?
show the reaction diagram
methyl acetoin + 2,6-dichlorophenolindophenol
? + ?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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The combination of purified Ao:DCPIP OR, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase in the presence of thiamine diphosphate and the substrate acetoin or methylacetoin results in a coenzyme A-dependent reduction of NAD+
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0028
acetoin
E1 complex, 80°C, pH 7.0; E1 complex, 80°C, pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.15
Alcaligenes eutrophus H16
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
136000
Alcaligenes eutrophus H16
gel filtration; gel filtration
138000
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gel filtration, component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase
142000
gel filtration, E1 complex; gel filtration, E1 complex
143000 - 177000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase
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from acetoin-grown cells
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from acetoin-grown cells; from acetoin-grown cells
Alcaligenes eutrophus H16
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
component E1alpha; component E1beta
expression in Escherichia coli
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expression in Escherichia coli; expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
formation of the enzyme components of the acetoin dehydrogenase enzyme system E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase Ao:DCPIP OR,, E2, i.e. dihydrolipoamide acetyltransferase DHLTA, and E3, i.e. dihydrolipoamide dehydrogenase DHLDH, are induced during growth on acetoin
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the enzymes Ao:DCPIP OR, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase are induced during growth on acetoin, whereas they are absent or scarcely present in cells grown on a nonacetoinogenic substrate
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