Information on EC 2.3.1.174 - 3-oxoadipyl-CoA thiolase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
2.3.1.174
-
RECOMMENDED NAME
GeneOntology No.
3-oxoadipyl-CoA thiolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl-CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
Acyl group transfer
Pseudomonas sp. B13
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
3-oxoadipate degradation
-
4-methylcatechol degradation (ortho cleavage)
-
Benzoate degradation
-
benzoyl-CoA degradation I (aerobic)
-
Microbial metabolism in diverse environments
-
phenylacetate degradation I (aerobic)
-
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:acetyl-CoA C-succinyltransferase
The enzyme from the bacterium Escherichia coli also has the activity of EC 2.3.1.223 (3-oxo-5,6-dehydrosuberyl-CoA thiolase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
mmlC
D2I940
gene encoding for a 3-oxoadipyl-CoA thiolase
mmlC
Pseudomonas reinekei MT1
D2I940
gene encoding for a 3-oxoadipyl-CoA thiolase
-
CAS REGISTRY NUMBER
COMMENTARY
403496-07-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain MT1
TREMBL
Manually annotated by BRENDA team
Pseudomonas reinekei MT1
strain MT1
TREMBL
Manually annotated by BRENDA team
strain B13
-
-
Manually annotated by BRENDA team
Pseudomonas sp. B13
strain B13
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-3-oxoadipyl-CoA + CoA
methylsuccinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas reinekei, Pseudomonas reinekei MT1
D2I940
-
-
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
-
-
-
-
-
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
-
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp. B13
-
-
-
-
-
CoA + oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-methyl-3-oxoadipyl-CoA + CoA
methylsuccinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas reinekei, Pseudomonas reinekei MT1
D2I940
-
-
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NaCN
-
1 mM, 12% inhibition
NADH
-
10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM
ZnCl2
-
1 mM, 46% inhibition
CuSO4
-
1 mM, 90% inhibition
additional information
-
1 mM EDTA or 1,10-phenanthroline have no effect
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.15
-
3-oxoadipyl-CoA
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
7.8
-
3-oxoadipyl-CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
11.1
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
162000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 41678, calculation from nucleotide sequence
?
Pseudomonas sp. B13
-
x * 41678, calculation from nucleotide sequence
-
tetramer
-
4 * 42000, SDS-PAGE
tetramer
Pseudomonas sp. B13
-
4 * 42000, SDS-PAGE
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
D2I940
generation of a mmlC knockout strain
additional information
Pseudomonas reinekei MT1
-
generation of a mmlC knockout strain
-