Information on EC 2.3.1.174 - 3-oxoadipyl-CoA thiolase

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The expected taxonomic range for this enzyme is: Proteobacteria

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.3.1.174
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
3-oxoadipyl-CoA thiolase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl-CoA
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
Acyl group transfer
Pseudomonas sp. B13
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-oxoadipate degradation
-
-
4-methylcatechol degradation (ortho cleavage)
-
-
benzoyl-CoA degradation I (aerobic)
-
-
phenylacetate degradation I (aerobic)
-
-
phenylacetat degradation (aerobic)
-
-
Phenylalanine metabolism
-
-
Benzoate degradation
-
-
Microbial metabolism in diverse environments
-
-
SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
succinyl-CoA:acetyl-CoA C-succinyltransferase
The enzyme from the bacterium Escherichia coli also has the activity of EC 2.3.1.223 (3-oxo-5,6-dehydrosuberyl-CoA thiolase).
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
mmlC
gene encoding for a 3-oxoadipyl-CoA thiolase
mmlC
Pseudomonas reinekei MT1
gene encoding for a 3-oxoadipyl-CoA thiolase
-
SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
403496-07-1
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain MT1
TREMBL
Manually annotated by BRENDA team
Pseudomonas reinekei MT1
strain MT1
TREMBL
Manually annotated by BRENDA team
strain B13
-
-
Manually annotated by BRENDA team
Pseudomonas sp. B13
strain B13
-
-
Manually annotated by BRENDA team
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-3-oxoadipyl-CoA + CoA
methylsuccinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas reinekei, Pseudomonas reinekei MT1
-
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
-
-
-
-
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp. B13
-
-
-
-
CoA + oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
-
-
?
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-methyl-3-oxoadipyl-CoA + CoA
methylsuccinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas reinekei, Pseudomonas reinekei MT1
D2I940
-
-
?
CoA + 3-oxoadipyl-CoA
succinyl-CoA + acetyl-CoA
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. B13
-
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NaCN
-
1 mM, 12% inhibition
NADH
-
10% inhibition with 0.4 mM, 42% inhibition by 0.8 mM
ZnCl2
-
1 mM, 46% inhibition
CuSO4
-
1 mM, 90% inhibition
additional information
-
1 mM EDTA or 1,10-phenanthroline have no effect
-
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.15
3-oxoadipyl-CoA
-
-
0.01
CoA
-
-
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
7.8
3-oxoadipyl-CoA
Pseudomonas sp.
-
-
7.8
CoA
Pseudomonas sp.
-
-
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
162000
-
gel filtration
646002
SplaateSubunits, Organism, Commentary, Literature
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 41678, calculation from nucleotide sequence
?
Pseudomonas sp. B13
-
x * 41678, calculation from nucleotide sequence
-
tetramer
-
4 * 42000, SDS-PAGE
tetramer
Pseudomonas sp. B13
-
4 * 42000, SDS-PAGE
-
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
generation of a mmlC knockout strain
additional information
Pseudomonas reinekei MT1
-
generation of a mmlC knockout strain
-
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature