Information on EC 2.3.1.137 - carnitine O-octanoyltransferase

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The expected taxonomic range for this enzyme is: Opisthokonta

EC NUMBER
COMMENTARY
2.3.1.137
-
RECOMMENDED NAME
GeneOntology No.
carnitine O-octanoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine
show the reaction diagram
-
-
-
-
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine
show the reaction diagram
catalytic mechanism, active site structure, substrate recognition mechanism, amino acid residues 343, 564 and 553 determine the acyl-CoA substrate specificity, catalytic residue is His343
-
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine
show the reaction diagram
acyl-CoA substrate specificity is determined by Gly553, active site structure and substrate positioning modeling
-
octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine
show the reaction diagram
acyl-binding hydrophobic pocket structure involving residues G553, C323, and Met335, the acyl-CoA substrate chain length specificity is determined by G553, Met335 is important for catalysis
Q9DC50
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
Acyl group transfer
-
-
Acyl group transfer
Mus musculus Sv/129
-
-
-
SYSTEMATIC NAME
IUBMB Comments
octanoyl-CoA:L-carnitine O-octanoyltransferase
Acts on a range of acyl-CoAs, with optimal activity with C6 or C8 acyl groups. cf. EC 2.3.1.7 (carnitine O-acetyltransferase) and EC 2.3.1.21 (carnitine O-palmitoyltransferase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carnitine acyltransferase
-
-
carnitine acyltransferase
Mus musculus Sv/129
-
-
-
Carnitine medium-chain acyltransferase
-
-
-
-
carnitine octanoyltransferase
-
-
carnitine octanoyltransferase
-
-
carnitine octanoyltransferase
-
; CROT
carnitine octanoyltransferase
Mus musculus Sv/129
-
; CROT
-
carnitine octanoyltransferase
-
-
COT
Rattus norvegicus Sprague-Dawley
-
;
-
COT
Rattus norvegicus Wistar
-
-
-
CRAT
-
-
CRAT
Mus musculus Sv/129
-
-
-
CrOT
-
-
CrOT
Q9UKG9
-
CrOT
Mus musculus Sv/129
-
-
-
easily solubilized mitochondrial carnitine palmitoyltransferase
-
-
-
-
medium-chain carnitine acyltransferase
-
-
overt mitochondrial carnitine palmitoyltransferase
-
-
-
-
short-chain carnitine acyltransferase
-
-
medium-chain/long-chain carnitine acyltransferase
-
-
-
-
additional information
-
enzyme belongs to the family of carnitine acyltransferases
additional information
-
the enzyme belongs to the carnitine acyltransferase family
CAS REGISTRY NUMBER
COMMENTARY
39369-19-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
beef; bovine
-
-
Manually annotated by BRENDA team
beef; calf
-
-
Manually annotated by BRENDA team
bovine
SwissProt
Manually annotated by BRENDA team
bovine
-
-
Manually annotated by BRENDA team
bovine; ox
-
-
Manually annotated by BRENDA team
SwissProt accession number U65745
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
GenBank accession number AF168793
SwissProt
Manually annotated by BRENDA team
HeLa cells
-
-
Manually annotated by BRENDA team
fed lipid-lowering drug diet containing clofibrate, nafenopin or Wy-14,643
-
-
Manually annotated by BRENDA team
feeding of phytol results in induction of carnitine O-octanoyltransferase as well as catalase, phytanoyl-CoA hydroxylase, peroxisomal 3-ketoacyl-CoA thiolase, and straight-chain acyl-CoA oxidase
-
-
Manually annotated by BRENDA team
male Sv/129 mice; strain Sv/129
-
-
Manually annotated by BRENDA team
NMRI/Bom strain
-
-
Manually annotated by BRENDA team
Mus musculus NMRI/Bom
NMRI/Bom strain
-
-
Manually annotated by BRENDA team
Mus musculus Sv/129
strain Sv/129
-
-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
-
-
-
Manually annotated by BRENDA team
rat; Sprague-Dawley
-
-
Manually annotated by BRENDA team
rat; Wistar
-
-
Manually annotated by BRENDA team
rat; Wistar/Moell
-
-
Manually annotated by BRENDA team
SwissProt accession number U26033
SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar/Moell
Wistar/Moell
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
Q9UKG9
overexpression and knock-down of the enzyme gene in a model of hepatic cells. Increase in enzymic activity induces a decrease in medium chain fatty acid and very long chaichain fatty acid levels in the cell. These changes are accompanied by an increase in the level of mRNA encoding enzymes of the peroxisomal beta oxidation, while there is no change in mitochondrial function. A decrease in carnitine ocatanoyltransferase activity has the opposite effect. Results suggest that carnitine ocatanoyltransferase activity, by controlling the peroxisomal amount of medium chain acyls, may control the peroxisomal oxidative pathway
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-methylglutaryl-CoA + L-carnitine
3-methylglutaryl-L-carnitine + CoA
show the reaction diagram
-
little capacity to use this substrate
-
-
r
3-methylglutaryl-L-carnitine + CoA
3-methyglutaryl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
4,8-dimethylnonanoyl-CoA + L-carnitine
4,8-dimethylnonanoyl-L-carnitine + CoA
show the reaction diagram
Q9UKG9
-
-
-
r
4,8-dimethylnonanoyl-L-carnitine + CoA
4,8-dimethylnonanoyl-CoA + L-carnitine
show the reaction diagram
Q9UKG9
-
-
-
r
acetoacetyl-CoA + L-carnitine
acetoacetyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acetoacetyl-L-carnitine + CoA
acetoacetyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acetyl-CoA + L-carnitine
acetyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acetyl-CoA + L-carnitine
acetyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acetyl-CoA + L-carnitine
CoA + L-acetylcarnitine
show the reaction diagram
Q9DC50
low activity
-
-
?
acetyl-CoA + L-carnitine
CoA + L-acetylcarnitine
show the reaction diagram
-
low activity
-
-
?
acetyl-CoA + L-carnitine
CoA + acetyl-carnitine
show the reaction diagram
Mus musculus, Mus musculus Sv/129
-
-
-
-
?
acetyl-L-carnitine + CoA
acetyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acetyl-L-carnitine + CoA
acetyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
P11466
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Q9UKG9
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
P11466
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
gamma-trimethylamino-beta-hydroxybutyrate
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
O19094
central role in fatty acid metabolism
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
metabolic pathway of peroxisomal beta-oxidation in yeasts
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
beta-oxidation of fatty acids
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-, gamma-trimethylamino-beta-hydroxybutyrate
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
O19094
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
beta-hydroxy-beta-methylglutaryl-CoA + L-carnitine
beta-hydroxy-beta-methylglutaryl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
beta-hydroxy-beta-methylglutaryl-L-carnitine + CoA
beta-hydroxy-beta-methylglutaryl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
butanoyl-CoA + L-carnitine
CoA + L-butanoylcarnitine
show the reaction diagram
-
-
-
-
?
butyryl-CoA + L-carnitine
butyryl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
butyryl-L-carnitine + CoA
butyryl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
choline + L-carnitine
?
show the reaction diagram
-
-
-
-
-
choline + L-carnitine
?
show the reaction diagram
-
extremely poor substrate
-
-
?
decanoyl-CoA + L-carnitine
L-decanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
decanoyl-CoA + L-carnitine
L-decanoyl-L-carnitine + CoA
show the reaction diagram
Q9UKG9
-
-
-
r
decanoyl-CoA + L-carnitine
L-decanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
decanoyl-CoA + L-carnitine
CoA + L-decanoylcarnitine
show the reaction diagram
-
best substrate
-
-
?
decanoyl-L-carnitine + CoA
decanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
decanoyl-L-carnitine + CoA
decanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
-
decanoyl-L-carnitine + CoA
decanoyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
dodecanoyl-CoA + L-carnitine
L-dodecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
dodecanoyl-CoA + L-carnitine
L-dodecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
dodecanoyl-CoA + L-carnitine
L-dodecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
dodecanoyl-CoA + L-carnitine
L-dodecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
dodecanoyl-CoA + L-carnitine
L-dodecanoyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
dodecanoyl-CoA + L-carnitine
CoA + L-dodecanoylcarnitine
show the reaction diagram
-
-
-
-
?
dodecanoyl-L-carnitine + CoA
dodecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
dodecanoyl-L-carnitine + CoA
dodecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
dodecanoyl-L-carnitine + CoA
dodecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
dodecanoyl-L-carnitine + CoA
dodecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
dodecanoyl-L-carnitine + CoA
dodecanoyl-CoA + L-carnitine
show the reaction diagram
O19094
-
-
-
r
heptanoyl-CoA + L-carnitine
L-heptanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
heptanoyl-CoA + L-carnitine
L-heptanoyl-L-carnitine + CoA
show the reaction diagram
Q9UKG9
-
-
-
r
heptanoyl-CoA + L-carnitine
L-heptanoyl-L-carnitine + CoA
show the reaction diagram
Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
heptanoyl-L-carnitine + CoA
heptanoyl-CoA + L-carnitine
show the reaction diagram
Mus musculus, Rattus norvegicus, Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
hexadecanoyl-CoA + L-carnitine
L-hexadecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexadecanoyl-CoA + L-carnitine
L-hexadecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexadecanoyl-CoA + L-carnitine
L-hexadecanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexadecanoyl-CoA + L-carnitine
CoA + L-hexadecanoylcarnitine
show the reaction diagram
-
low activity
-
-
?
hexadecanoyl-L-carnitine + CoA
hexadecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexadecanoyl-L-carnitine + CoA
hexadecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexadecanoyl-L-carnitine + CoA
hexadecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexadecanoyl-L-carnitine + CoA
hexadecanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexadecanoyl-L-carnitine + CoA
hexadecanoyl-CoA + L-carnitine
show the reaction diagram
O19094
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
hexanoyl-CoA + L-carnitine
L-hexanoyl-L-carnitine + CoA
show the reaction diagram
Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
hexanoyl-CoA + L-carnitine
CoA + L-hexanoylcarnitine
show the reaction diagram
-
best substrate
-
-
?
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
O19094
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
hexanoyl-L-carnitine + CoA
hexanoyl-CoA + L-carnitine
show the reaction diagram
Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
-, r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
malonyl-CoA + L-carnitine
malonyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
myristoyl-CoA + L-carnitine
myristoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
myristoyl-L-carnitine + CoA
myristoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
-
-
-
?
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
Q9DC50
-
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
-
-
-
?
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
Q9DC50
preferred substrate
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio, carnitine binding site structure involving the highly conserved R518, Y452 and T465, CoA binding site n the active site tunnel via His343, fatty acid binding site
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
Mus musculus Sv/129
-
-
-
-
?
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
O19094
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
reverse reaction
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
palmitoyl-CoA + L-carnitine
L-palmitoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
palmitoyl-CoA + L-carnitine
L-palmitoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
palmitoyl-CoA + L-carnitine
L-palmitoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
palmitoyl-L-carnitine + CoA
palmitoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
palmitoyl-L-carnitine + CoA
palmitoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
palmitoyl-L-carnitine + CoA
palmitoyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
-
palmitoyl-L-carnitine + CoA
palmitoyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
pivaloyl-CoA + L-carnitine
L-pivaloyl-L-carnitine + CoA
show the reaction diagram
-
little capacity to use this substrate
-
-
r
pivaloyl-L-carnitine + CoA
pivaloyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
propionyl-CoA + L-carnitine
propionyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
propionyl-CoA + L-carnitine
propionyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
propionyl-CoA + L-carnitine
CoA + propionyl-carnitine
show the reaction diagram
Mus musculus, Mus musculus Sv/129
-
-
-
-
?
propionyl-L-carnitine + CoA
propionyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
propionyl-L-carnitine + CoA
propionyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
tetradecanoyl-CoA + L-carnitine
CoA + L-tetradecanoylcarnitine
show the reaction diagram
-
-
-
-
?
tetradecanoyl-CoA + L-carnitine
CoA + L-tetradecanoylcarnitine
show the reaction diagram
Q9DC50
low activity
-
-
?
valproyl-CoA + L-carnitine
L-valproyl-L-carnitine + CoA
show the reaction diagram
-
little capacity to use this substrate
-
-
r
valproyl-L-carnitine + CoA
valproyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
malonyl-L-carnitine + CoA
malonyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
additional information
?
-
Q9UKG9
2,6-dimethyl-heptanoyl-CoA is not a substrate
-
-
-
additional information
?
-
-
malonyl-CoA is not a substrate
-
-
-
additional information
?
-
-
palmitoyl-CoA is no substrate
-
-
-
additional information
?
-
-
succinyl-CoA and beta-hydroxy-beta-methylglutaryl-CoA are no substrates
-
-
-
additional information
?
-
-
acyl-CoA substrate specificity is determined by Gly553
-
-
-
additional information
?
-
-
peroxisomal carnitine acyltransferases and acyl-CoA thioesterases are differently expressed to function as complementary enzymes for transport of beta-oxidation metabolites, peroxisomes contain carnitine acetyltransferase and octanoyltransferase that produce carnitine ester for transport out of the peroxisomes
-
-
-
additional information
?
-
Mus musculus Sv/129
-
peroxisomal carnitine acyltransferases and acyl-CoA thioesterases are differently expressed to function as complementary enzymes for transport of beta-oxidation metabolites, peroxisomes contain carnitine acetyltransferase and octanoyltransferase that produce carnitine ester for transport out of the peroxisomes
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-carnitine
CoA + acetyl-carnitine
show the reaction diagram
Mus musculus, Mus musculus Sv/129
-
-
-
-
?
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
P11466
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Q9UKG9
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
P11466
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
O19094
central role in fatty acid metabolism
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
metabolic pathway of peroxisomal beta-oxidation in yeasts
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
-
beta-oxidation of fatty acids
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Mus musculus NMRI/Bom, Rattus norvegicus Wistar/Moell
-
-
-
-
r
acyl-CoA + L-carnitine
acyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
O19094
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
acyl-L-carnitine + CoA
acyl-CoA + L-carnitine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
-
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
-
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
O19094
-
-
-
r
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
-
-
-
?
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
Q9DC50
-
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
-
-
-
?
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
-
step in the transport of fatty acids across the inner mitochondrial membrane, reaction plays a role in balancing cellular CoA:acyl-CoA ratio
-
-
r
octanoyl-CoA + L-carnitine
CoA + L-octanoylcarnitine
show the reaction diagram
Mus musculus Sv/129
-
-
-
-
?
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
O19094
-
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
-
reverse reaction
-
-
r
octanoyl-L-carnitine + CoA
L-carnitine + octanoyl-CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
propionyl-CoA + L-carnitine
CoA + propionyl-carnitine
show the reaction diagram
Mus musculus, Mus musculus Sv/129
-
-
-
-
?
L-carnitine + octanoyl-CoA
octanoyl-L-carnitine + CoA
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
additional information
?
-
-
peroxisomal carnitine acyltransferases and acyl-CoA thioesterases are differently expressed to function as complementary enzymes for transport of beta-oxidation metabolites, peroxisomes contain carnitine acetyltransferase and octanoyltransferase that produce carnitine ester for transport out of the peroxisomes
-
-
-
additional information
?
-
Mus musculus Sv/129
-
peroxisomal carnitine acyltransferases and acyl-CoA thioesterases are differently expressed to function as complementary enzymes for transport of beta-oxidation metabolites, peroxisomes contain carnitine acetyltransferase and octanoyltransferase that produce carnitine ester for transport out of the peroxisomes
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-bromodecanoic acid
-
-
2-bromooctanoic acid
-
-
2-bromopalmitic acid
-
potent inhibitor
2-Bromopalmitoyl-CoA
-
-
Aminocarnitine
-
-
D-carnitine
-
-
Dodecanoyl-CoA
-
substrate inhibition
etomoxir
-
hypoglycaemia-inducing drug, double mutant H131A/H340A is insensitive to etomoxir
hemiacylcarnitinium
-
-
hemicholinium
-
-
hexadecanoyl-CoA
-
substrate inhibition
malonyl-CoA
-
inhibits the purified enzyme, inhibition is affected by buffer, pH, substrate and presence or absence of bovine serum albumin
malonyl-CoA
-
microsomal COT, no inhibition of the mitochondrial enzyme
malonyl-CoA
-
double mutant H131A/H340A is insensitive to malonyl-CoA
malonyl-CoA
-
regulatory role, enzyme belongs to the group of malonyl-CoA inhibitable carnitine acyltransferases with highly conserved residues being involved in the inhibition, i.e. Thr314, Asn464, Ala478, Met593, and Cys608, amino acid sequence comparison with enzymes from other species
Octanoyl-CoA
-
substrate inhibition
palmitoyl-CoA
-
-
tetradecylglycidic acid
-
-
Trypsin
-
-
-
Zn2+
-
inhibits enzyme in reverse direction
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
retains its maximum activity when preincubated at pH 7.0 or 8.5
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.155
-
acetyl-CoA
-
-
16
-
acetyl-CoA
-
-
0.783
-
acetyl-L-carnitine
-
-
0.002
-
acyl-CoA
-
-
0.0167
-
acyl-CoA
-
mutant strain expressed in Saccharomyces cerevisiae COT A238D
0.0948
-
carnitine
O19094
mutant ATS-COT
0.0948
-
carnitine
-
-
143
-
choline
-
mutant [Asn505]COT
0.11
-
CoASH
-
-
0.0002
-
Decanoyl-CoA
-
-
0.000206
-
Decanoyl-CoA
O19094
mutant AAA-COT
0.00024
-
Decanoyl-CoA
-
pH 6.8
0.00024
-
Decanoyl-CoA
-
mutant [Asn505]COT
0.00034
-
Decanoyl-CoA
-
pH 7.4
0.00138
-
Decanoyl-CoA
O19094
purified enzyme, wild-type COT
0.00138
-
Decanoyl-CoA
-
purified enzyme, wild-type COT
0.00139
-
Decanoyl-CoA
O19094
mutant ATS-COT
0.002
-
Decanoyl-CoA
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTwt
0.002
-
Decanoyl-CoA
P11466
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTwt
0.0026
-
Decanoyl-CoA
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH340A/H131
0.00588
-
Decanoyl-CoA
O19094
mutant AAS-COT
0.006
-
Decanoyl-CoA
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH340
0.00762
-
Decanoyl-CoA
O19094
mutant SAS-COT
0.0107
-
Decanoyl-CoA
P11466
-
0.013
-
Decanoyl-CoA
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH340A/H131
0.0513
-
Decanoyl-CoA
O19094
mutant STA-COT
0.0819
-
Decanoyl-CoA
O19094
wild-type COT
0.303
-
Decanoyl-CoA
O19094
mutant SAA-COT
0.34
-
Decanoyl-CoA
-
-
0.373
-
Decanoyl-CoA
O19094
mutant ATA-COT
0.794
-
Decanoyl-CoA
O19094
mutant AAA-COT
8.4
-
Decanoyl-L-carnitine
-
-
0.036
-
hexadecanoyl-L-carnitine
-
-
0.102
-
L-carnitine
-
pH 7.4
0.106
-
L-carnitine
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH340A
0.106
-
L-carnitine
P11466
mutant A332G
0.117
-
L-carnitine
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH340A/H131
0.13
-
L-carnitine
-
-
0.172
-
L-carnitine
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTwt
0.172
-
L-carnitine
P11466
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTwt
0.218
-
L-carnitine
-
-
0.227
-
L-carnitine
-
pH 6.8
0.992
-
L-carnitine
O19094
mutant AAS-COT
108
-
L-carnitine
-
-
146
-
L-carnitine
-
recombinant enzyme expressed in Saccharomyces cerevisiae COT238D
160
-
L-carnitine
-
mutant [Asn505]COT
160
-
L-carnitine
-
recombinant enzyme expressed in Saccharomyces cerevisiae pYESCOTH131A
0.015
-
Octanoyl-CoA
-
-
0.1
-
octanoylcarnitine
-
-
0.104
-
palmitoyl lysophosphatidic acid
-
-
0.00046
-
palmitoyl-CoA
-
peroxisomal COT
0.00047
-
palmitoyl-CoA
-
pH 6.8
0.0006
-
palmitoyl-CoA
-
purified COT, pH 7.4
0.069
-
palmitoyl-CoA
-
-
0.6
-
palmitoyl-CoA
O19094
mutant SAA-COT
7.4
-
palmitoyl-L-carnitine
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.367
-
Octanoyl-CoA
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.84
-
D-carnitine
-
-
113
-
hemiacylcarnitinium
-
-
0.0035
-
malonyl-CoA
-
pH 6.8
0.0064
-
malonyl-CoA
-
peroxisomal COT
0.106
-
malonyl-CoA
-
pH 7.4
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.072
-
-
-
additional information
-
-
acyl-CoA substrate specificity of wild-type and G553M mutant enzymes, overview
additional information
-
Q9DC50
substrate specificity of wild-type and mutant enzymes, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.8
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
10
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Mus musculus Sv/129, Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
high expression of CRAT
Manually annotated by BRENDA team
Q9UKG9
skin fibroblast
Manually annotated by BRENDA team
-
high expression of CRAT
Manually annotated by BRENDA team
Mus musculus Sv/129
-
high expression of CRAT
-
Manually annotated by BRENDA team
-
proximal and distal
Manually annotated by BRENDA team
Mus musculus Sv/129
-
-
-
Manually annotated by BRENDA team
Mus musculus NMRI/Bom, Mus musculus Sv/129, Rattus norvegicus Sprague-Dawley, Rattus norvegicus Wistar/Moell
-
-
-
Manually annotated by BRENDA team
-
breast muscle
Manually annotated by BRENDA team
Mus musculus Sv/129
-
-
-
Manually annotated by BRENDA team
-
high expression of CRAT
Manually annotated by BRENDA team
additional information
-
low in distal intestine epithelium, distal intestine muscle, brain, heart, lung, spleen, brown and white adipose tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Sprague-Dawley
-
rough and smooth
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
;
-
-
Manually annotated by BRENDA team
-
innermitochondrial membrane
Manually annotated by BRENDA team
-
synaptic and non-synaptic mitochondria
Manually annotated by BRENDA team
-
; the crat gene contains 2 alternative start codons, translation from the first codon results in a protein of 626 amino acids, with a 21-amino acid leader sequence that targets the CRAT protein to mitochondria
Manually annotated by BRENDA team
Mus musculus NMRI/Bom
-
innermitochondrial membrane
-
Manually annotated by BRENDA team
Mus musculus Sv/129
-
; the crat gene contains 2 alternative start codons, translation from the first codon results in a protein of 626 amino acids, with a 21-amino acid leader sequence that targets the CRAT protein to mitochondria
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
synaptic and non-synaptic mitochondria
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar/Moell
-
innermitochondrial membrane
-
Manually annotated by BRENDA team
-
matrix of liver peroxisomes
Manually annotated by BRENDA team
-
; in the peroxisomal CRAT mRNA, there is an in-frame stop codon and translation starts at a downstream second start codon, resulting in a protein that contains 605 amino acids
Manually annotated by BRENDA team
Mus musculus Sv/129
-
; in the peroxisomal CRAT mRNA, there is an in-frame stop codon and translation starts at a downstream second start codon, resulting in a protein that contains 605 amino acids
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
55000
-
SDS-PAGE
53000
-
-
Western blot, polyclonal antibody reaction
59000
-
-
gel filtration
60000
-
-
gel filtration
64400
-
-
SDS-PAGE
66000
-
-
gel filtration
66000
-
Q9UKG9
recombinant enzyme, expressed in Saccharomyces cerevisiae
70000
-
-
gel filtration
70000
-
Q9UKG9
calculated from cDNA sequence
70260
-
-
calculated from cDNA sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 69000, recombinant enzyme, SDS-PAGE
monomer
-
1 * 60000, SDS-PAGE
monomer
-
-
monomer
-
1 * 64400, SDS-PAGE
monomer
Q9UKG9
1 * 66000, SDS-PAGE
additional information
-
active site structure and substrate positioning modeling for wild-type and G553M mutant enzymes
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified recombinant His-tagged wild-type and mutants M335V and C323M enzymes free or in complex with octanoylcarnitine, sitting drop vapour diffusion method, 4C, reservoir solution contains 0.1 M HEPES, pH 7.4, 62% v/v 2-methyl-2,4-pentanediol, larger crystals by micro- and macroseeding, cross-seeding of recombinant purified selenomethionine-labeled enzyme, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution
Q9DC50
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
quite unstable, labile in Triton X-100 and octylglucoside, stable in 8 mM CHAPS, 200 mM guanidium chloride and 0.5% Tween 20
-
stable for at least 1 week in 100 mM phosphate buffer with 0.4 M KCl, pH 5.5-7.5
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-18C, can be kept for several months with only moderate loss of activity
-
-20C, freezing causes a 90% loss of activity within 2 days
-
-80C, freezing causes a 90% loss of activity within 2 days
-
20C, 10 mM sodium diphosphate buffer at pH 7.5, stable for months
-
4C, 10 mM sodium diphosphate buffer at pH 7.5, stable for months
-
-70C, stable to freezing at for at least 5 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially
-
recombinant enzyme
-
soluble recombinant His-tagged wild-type and selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography
Q9DC50
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cDNA cloning by library screening and recombinant expression in Escherichia coli
-
cloned and expressed in Escherichia coli
O19094
cDNA PCR amplified, cloned in yeast expression vector pEL26 and expressed in Saccharomyces cerevisiae
Q9UKG9
expression in HepG2 cell
Q9UKG9
overexpression of the His-tagged enzyme in Escherichia coli as soluble protein, expression of enzyme for selenomethionine-labeling in Escherichia coli strain B834(DE3)
Q9DC50
cDNA clone from a rat liver library, 2 forms of COT in peroxisomes results of the trans-splicing mechanism in pre-mRNAs
-
expression of wild-type and G553M mutant enzymes in an enzyme-deficient Saccharomyces cerevisiae strain
-
pYES2 plasmid containing the wild-type and the double mutant H131A/H340A of COT expressed in Saccharomyces cerevisiae
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
R518N
-
site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme
S552A
-
site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme
S554X
-
site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme
C323M
Q9DC50
site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, altered acyl-CoA substrate specificity compared to the wild-type enzyme, highly increased specificity for octanoyl-CoA
G553M
Q9DC50
site-directed mutagenesis, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme
M335A
Q9DC50
site-directed mutagenesis, reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme
M335V
Q9DC50
site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme
G553M
-
site-directed mutagenesis, the mutant enzyme shows altered substrate specificity: highly reduced activity with medium- and long-chain acyl-CoAs and increased activity with acetyl-CoA and butanoyl-CoA compared to the wild-type enzyme
additional information
-
the carnitine acetyl-transferase, specific for acetyl-CoA/short-chain acyl-CoAs, can be modified to an enzyme with elevated carnitine octanoyltransferase activity by mutation of Met564 to Gly, overview