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Information on EC 2.3.1.129 - acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SU91

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IUBMB Comments
Involved with EC 2.4.1.182, lipid-A-disaccharide synthase, and EC 2.7.1.130, tetraacyldisaccharide 4'-kinase, in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of Gram-negative bacteria.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9SU91
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
udp-n-acetylglucosamine acyltransferase, lilpxa, udp-n-acetylglucosamine 3-o-acyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-N-acetylglucosamine acyltransferase
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acyltransferase, uridine diphosphoacetylglucosamine
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UDP-N-acetylglucosamine acyltransferase
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uridine diphosphoacetylglucosamine acyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
(3R)-3-hydroxyacyl-[acyl-carrier protein]:UDP-N-acetyl-alpha-D-glucosamine 3-O-(3-hydroxyacyl)transferase
Involved with EC 2.4.1.182, lipid-A-disaccharide synthase, and EC 2.7.1.130, tetraacyldisaccharide 4'-kinase, in the biosynthesis of the phosphorylated glycolipid, Lipid A, in the outer membrane of Gram-negative bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
105843-69-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
show the reaction diagram
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r
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the overall structure of AtLpxA is very similar to that of Escherichia coli LpxA, EcLpxA, with an alpha-helical-rich C-terminus and characteristic N-terminal left-handed parallel beta-helix. All key catalytic and chain length determining residues of EcLpxA are conserved in AtLpxA,but AtLpxA has an additional coil and loop added to the N-terminal left-handed parallel beta-helix not seen in EcLpxA
metabolism
LpxA catalyzes the first step in lipid A biosynthesis, pathway overview
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
LPXA_ARATH
336
0
36714
Swiss-Prot
Mitochondrion (Reliability: 5)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant LpxA, sitting drop vapor diffusion method, mixing of AtLpxA at 18 mg/mL in 10 mM potassium phosphate buffer, pH 7.0, 200 mM KCl, and 20% glycerol, in a 1:1 ratio with precipitant solution containing 0.5 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6, and 1.0 M lithium sulfate monohydrate, equilibration at 20°C, 1-2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant LpxA, excluding the region encoding the predicted signal peptide (residues 2-32), from Escherichia coli strain C41(DE3) by Reactive Green 19 affinity chromatography and gel filtration to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene lpxA, excluding the region encoding the predicted signal peptide (residues 2-32), expression of the plant enzyme in Escherichia coli strain C41(DE3). Functional expression of AtlpxA in and complementation of the constructed lpxA Escherichia coli knockout mutant strains
gene lpxA, five AtLpxC orthologues located in a 68-kb region on chromosome one, DNA and amino acid sequence determination and analysis, sequence comparison
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Joo, S.H.; Chung, H.S.; Raetz, C.R.; Garrett, T.A.
Activity and crystal structure of Arabidopsis thaliana UDP-N-acetylglucosamine acyltransferase
Biochemistry
51
4322-4330
2012
Arabidopsis thaliana (Q9SU91), Arabidopsis thaliana
Manually annotated by BRENDA team
Li, C.; Guan, Z.; Liu, D.; Raetz, C.R.
Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli
Proc. Natl. Acad. Sci. USA
108
11387-11392
2011
Arabidopsis thaliana (Q9SU91)
Manually annotated by BRENDA team