Information on EC 2.3.1.107 - deacetylvindoline O-acetyltransferase

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The expected taxonomic range for this enzyme is: Catharanthus roseus

EC NUMBER
COMMENTARY hide
2.3.1.107
-
RECOMMENDED NAME
GeneOntology No.
deacetylvindoline O-acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + deacetylvindoline = CoA + vindoline
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Indole alkaloid biosynthesis
-
-
vindoline and vinblastine biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:deacetylvindoline 4-O-acetyltransferase
Catalyses the final step in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle, Catharanthus roseus.
CAS REGISTRY NUMBER
COMMENTARY hide
100630-41-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 17-O-deacetylvindoline
CoA + vindoline
show the reaction diagram
acetyl-CoA + deacetylvindoline
CoA + vindoline
show the reaction diagram
propionyl-CoA + 17-O-deacetylvindoline
CoA + 17-O-deacetyl-17-O-propionylvindoline
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + 17-O-deacetylvindoline
CoA + vindoline
show the reaction diagram
acetyl-CoA + deacetylvindoline
CoA + vindoline
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
10 mM, activity is enhanced by 18%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
minovincinine-19-O-acetyltransferase
-
incubation of DAT with varying amounts of crude recombinant minovincinine-19-O-acetyltransferase (MAT) inhibits DAT activity in a dose dependent manner
-
phytoregulator
-
-
-
secologanin
-
0.5 mM, 25% inhibition
tabersonine
-
0.045 mM, 50% inhibition
tryptamine
-
0.5 mM, 28% inhibition
vindoline
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
essential to maintain enzyme activity
jasmonate
-
enhances the vindoline accumulation in in vitro shoot cultures
additional information
-
16 h photoperiod treatment of shoot cultures followed by synchronized formation of new plantlets, peaks of activity at day 12, 20 and 28 after photoperiod treatment
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.025
acetyl-CoA
0.0013 - 0.03
deacetylvindoline
0.0007
vindoline
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
CoA
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00012
-
continuous light, day 0, 4 and 8; photoperiod, day 4
0.0003
-
continuous light, day 28, 32 and 36; photoperiod, day 8
0.0006
-
continuous light, day 24; photoperiod, day 0
0.0009
-
photoperiod, day 16 and 24
0.0015
-
photoperiod, day 12
0.0018
-
photoperiod, day 32
0.002
-
continuous light, day 16; photoperiod, day 20 and 28
0.0024
-
continuous light, day 12 and 20; photoperiod, day 36
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 11
-
activity increases from pH 5.5 to 7 and decreases rapidly between pH 9 and 11
6.5 - 9.5
-
about 50% of maximal activity, pH 9.5: about 55% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of light-treated etiolated seedlings
Manually annotated by BRENDA team
-
iodioblasts and laticifer of the bud
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
GFP-tagged DAT is localized to the cytoplasm and the nucleus
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme is no hetero- or homodimer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme is expressed as recombinant His-tagged protein in Escherichia coli
-
expressed in Escherichia coli and Catharanthus roseus
-
expression in Escherichia coli
-
gene Dat, DNA sequence determination and analysis
transformation to Agrobacterium tumefaciens
-
transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
increased expression after treamtment of cell suspension with methyl-ester jasmonic acid
-
Pythium aphanidermatum homogenate and methyl jasmonate upregulate DAT production in cell suspension cultures, elicitation to callus and shoot induce enzyme expression, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
production of therapeutically valuable terpenoid indole alkaloids