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Information on EC 2.1.2.5 - glutamate formimidoyltransferase and Organism(s) Gallus gallus and UniProt Accession Q9YH58

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IUBMB Comments
The enzyme also catalyses formyl transfer from 5-formyltetrahydrofolate to L-glutamate. In eukaryotes, it occurs as a bifunctional enzyme that also has formimidoyltetrahydrofolate cyclodeaminase (EC 4.3.1.4) activity.
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This record set is specific for:
Gallus gallus
UNIPROT: Q9YH58
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The taxonomic range for the selected organisms is: Gallus gallus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
formiminotransferase-cyclodeaminase, glutamate formiminotransferase, glutamate formyltransferase, formiminoglutamic acid formiminotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formiminoglutamic acid formiminotransferase
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formiminoglutamic acid transferase
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formiminoglutamic formiminotransferase
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formiminotransferase, glutamate
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glutamate formiminotransferase
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glutamate formyltransferase
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formimino group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
5-formimidoyltetrahydrofolate:L-glutamate N-formimidoyltransferase
The enzyme also catalyses formyl transfer from 5-formyltetrahydrofolate to L-glutamate. In eukaryotes, it occurs as a bifunctional enzyme that also has formimidoyltetrahydrofolate cyclodeaminase (EC 4.3.1.4) activity.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-83-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-formiminoglutamate + tetrahydrofolate
5-formiminotetrahydrofolate + L-glutamate
show the reaction diagram
tetrahydrofolate + N-formimino-L-glutamate
5-formiminotetrahydrofolate + L-glutamate
show the reaction diagram
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-
?
additional information
?
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p60: bifunctional formiminotransferase cyclodeaminase, can mediate interaction between trans-Golgi network derived vesicles and microtubule in vitro, p60 may bind to the polyglutamate on microtubules, globular hydrophilic protein
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-formiminoglutamate + tetrahydrofolate
5-formiminotetrahydrofolate + L-glutamate
show the reaction diagram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pI: 6.13
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
chicken, several isoforms of p60, bifunctional formiminotransferase cyclodeaminase
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
DU249 chicken hepatoma cells, contain p60 but no measurable formiminotransferase activity, may be due to the low expression of p60
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures
Manually annotated by BRENDA team
localized on Golgi membranes as well as on some potentially Golgi complex-derived vesicular structures
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Manually annotated by BRENDA team
microtubule-binding protein
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FTCD_CHICK
541
0
59154
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59100
x * 59100, calculated from the amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 59100, calculated from the amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
p60, bifunctional formiminotransferase cyclodeaminase, is post-translationally modified
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purification from liver
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and sequencing of the cDNA coding for p60, the chicken homologue of bifunctional formiminotransferase cyclodeaminase, a protein of 541 amino acids
expressed in HeLa cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hennig, D.; Scales, S.J.; Moreau, A.; Murley, L.L.; De Mey, J.; Kreis, T.E.
A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules
J. Biol. Chem.
273
19602-19611
1998
Gallus gallus (Q9YH58), Meleagris gallopavo
Manually annotated by BRENDA team