Information on EC 2.1.2.2 - phosphoribosylglycinamide formyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.1.2.2
-
RECOMMENDED NAME
GeneOntology No.
phosphoribosylglycinamide formyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
formyl group transfer
-
-
-
-
formyl group transfer
-
-
formyl group transfer
-
-
PATHWAY
KEGG Link
MetaCyc Link
5-aminoimidazole ribonucleotide biosynthesis I
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Biosynthesis of secondary metabolites
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Metabolic pathways
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One carbon pool by folate
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Purine metabolism
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tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase
-
-
2-amino-N-ribosylacetamide 5'-phosphate transformylase
-
-
-
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5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase
-
-
-
-
GAR formyltransferase
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-
-
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GAR formyltransferase
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GAR synthetase
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GAR TFase
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-
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GAR TFase
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-
GAR transformylase
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-
-
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GARFT
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GART
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glycinamide ribonucleotide formyltransferase
Q599X3, Q599X4, Q599X5
-
glycinamide ribonucleotide formyltransferase
-
-
glycinamide ribonucleotide transformylase
-
-
-
-
glycinamide ribonucleotide transformylase
-
-
glycinamide ribonucleotide transformylase
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glycinamide ribotide formyltransferase
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N10-formyltetrahydrolate:2-amino-N-ribosylacetamide-5'-phosphate transformylase
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-
phosphoribosylglycinamide formyltransferase
-
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purN transformylase
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-
CAS REGISTRY NUMBER
COMMENTARY
9032-02-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
fragment
Q599X3, Q599X4, Q599X5
SwissProt
Manually annotated by BRENDA team
chinese hamster
-
-
Manually annotated by BRENDA team
strain B
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-
Manually annotated by BRENDA team
strain TX635
-
-
Manually annotated by BRENDA team
strains DH5alpha, BL21(DE3)
-
-
Manually annotated by BRENDA team
strains DH5alpha, BL21(DE3), TX680
-
-
Manually annotated by BRENDA team
Escherichia coli TX635
strain TX635
-
-
Manually annotated by BRENDA team
; strain PAO1
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-
Manually annotated by BRENDA team
fission yeast
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
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-
-
?
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyl-5,8-dideazafolate + beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyl-5,8-dideazafolate + N1-(5-phospho-D-ribosyl)glycinamide
?
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + (alpha,beta)-glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences
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-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
involved in pyoverdine synthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
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-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
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r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
transformylation reaction of purine biosynthesis
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
Escherichia coli TX635
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10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide
tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
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-
-
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10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide
tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
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-
-
?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
-
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?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
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?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
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?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
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-
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?
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
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-
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?
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + N5-hydroxyornithine
tetrahydrofolate + N2-formyl-N5-hydroxyornithine
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + N5-hydroxyornithine
tetrahydrofolate + N5-formyl-N5-hydroxyornithine
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + O-phosphonate carbocyclic glycinamide ribonucleotide
tetrahydrofolate + O-phosphonate-ribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
acetate + ATP
acetyl phosphate + ADP
show the reaction diagram
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
acetate + ATP
acetyl phosphate + ADP
show the reaction diagram
Escherichia coli TX635
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
beta-glycinamide ribonucleotide + 10-formyltetrahydrofolate
?
show the reaction diagram
-
-
-
-
?
beta-glycinamide ribonucleotide + ATP + formate
beta-formylglycinamide ribonucleotide + ADP
show the reaction diagram
-
assay at 25C
-
-
?
formate + ATP + beta-glycinamide ribonucleotide
beta-formylglycinamide ribonucleotide + ADP
show the reaction diagram
Escherichia coli, Escherichia coli TX635
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
N10-formyl-5-deazafolate + glycinamide ribonucleotide
formylglycinamide ribonucleotide + 5-deazafolate
show the reaction diagram
-
-
-
-
?
N10-formyl-8-deazafolate + glycinamide ribonucleotide
formylglycinamide ribonucleotide + 8-deazafolate
show the reaction diagram
-
-
-
-
?
N10-formyldideazafolate + 5-phospho-D-ribosylglycinamide
dideazafolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
N10-formylfolate + glycinamide ribonucleotide
folate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
N5,N10-anhydroformyl-5,6,7,8tetrahydrofolic acid + 2-amino-N-ribosylacetamide 5'-phosphate + H2O
formylglycinamide ribotide + tetrahydrofolic acid + 2-formamido-N-ribosylacetamide 5'-phosphate
show the reaction diagram
-
-
-
-
ir
N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O
N10-formyltetrahydrofolate + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
-
N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O
N10-formyltetrahydrofolate + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
?
N5,N10-anhydroformyltetrahydrofolic acid + glycinamide ribonucleotide
formylglycinamide ribonucleotide + ?
show the reaction diagram
-
-
-
-
?
inosinic acid + glycinamide ribotide + H2O
5-amino-4-imidazolecarboxamide ribotide + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
monofunctional enzyme
-
-
-
additional information
?
-
-
monofunctional enzyme
-
-
-
additional information
?
-
-
reaction does not occur if glycine, glycinamide or glycinamide riboside is substituted for glycinamide ribotide
-
-
-
additional information
?
-
-
glycinamide ribonucleotide nucleoside is no substrate
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
alpha-glycinamide ribonucleotide and glycinamide ribonucleotide nucleoside are neither substrates for no inhibitors
-
-
-
additional information
?
-
-
10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels
-
-
-
additional information
?
-
-
GAR transformylase activity copurifies with 5,10-methylene-, 5,10-methenyl, and 10-formyl-H4folate sythetase (combined) trifunctional enzyme, EC 6.3.4.3, EC 1.5.1.5 and EC 3.5.4.9
-
-
-
additional information
?
-
-
purified along with 10-formyltetrahydrofolate synthetase, uses both diastereomers of 5,10-methenyltetrahydrofolate, (+)-L-diastereomer is used 120 times faster than the (-)-L-diastereomer
-
-
-
additional information
?
-
Escherichia coli TX635
-
10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase, purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
involved in pyoverdine synthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
-
-
r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
transformylation reaction of purine biosynthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway
-
-
?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
10-formyl-5,8-dideazafolate
-
-
10-formyl-5,8-dideazafolate
-
-
5,10-methenyltetrahydrofolate
-
-
formate
-
second GAR transformylase using formate and ATP in place of 10-formyltetrahydrofolate
L-(+-)-5,10-methenyltetrahydrofolate
-
-
L-(-)-10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
only formyldonor
N10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Fe2+
-
expression regulated by amount of iron in the growth medium
K+
-
activity enhanced by
Mg2+
-
purT gene product
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(6R)-5,10-dideazatetrahydrofolate
-
lometrexol
(6R)-dideazatetrahydrofolate diglutamate
-
LY235340
(6R)-dideazatetrahydrofolate pentaglutamate
-
LY235542
(6R)-dideazatetrahydrofolate tetraglutamate
-
LY266978
(6R)-dideazatetrahydrofolate triglutamate
-
LY235337
(6S)-dideazatetrahydrofolate
-
LY243246
(6S)-N10-formyltetrahydrofolate
-
-
(alpha,beta)-hydroxyacetamide ribonucleotide
-
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
-
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
-
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
-
10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
specifically inhibits recombinant GAR Tfase, inactive against other folate-dependent enzymes, potent inhibitor of tumor cell proliferation
10-Acetyl-5,8-dideazafolate
-
-
10-Acetyl-5,8-dideazafolate
-
-
10-CF3CO-DDACTHF
-
-
10-formyl-5,8-dideazafolic acid hexaglutamate
-
-
10-formyl-DDACTHF
-
-
10-methanesulfonyl-5-DACTHF
-
-
10-methanesulfonyl-DDACTHF
-
-
10-methylthio-DDACTHF
-
-
10-trifluoroacetyl-DDACTHF
-
-
2',3'-dideoxy-2',3'-didehydro-carbocyclic glycinamide ribonucleotide
-
-
2',3'-dideoxy-carbocyclic glycinamide ribonucleotide
-
-
2',5'-furan-dideazatetrahydrofolate
-
LY222306
2',5'-thiophene dideazatetrahydrofolate
-
LY309887, diastereomer B
2',5'-thiophene dideazatetrahydrofolate
-
LY309886, diastereomer A; LY309887, diastereomer B
2-deaminodideazatetrahydrofolate
-
-
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
-
4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
-
5'-phosphonate carbocyclic glycinamide ribonucleotide
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolic acid
-
-
5,10-dideazafolic acid
-
-
5,10-dideazatetrahydrofolate
-
-
5,10-dideazatetrahydrofolate
-
-
5,10-dideazatetrahydrofolate
-
-
5,8-Dideazafolate
-
-
5,8-dideazafolate hexaglutamate
-
-
5,8-dideazapteroyl hexaglutamate
-
-
5-deazatetrahydrofolate
-
-
alpha,beta-hydroxyacetamideribonucleotide
-
-
alpha,beta-N-(hydroxyacetyl)-D-ribofuranosylamine
-
-
beta-thioglycinamide dideazafolate
-
-
dideazafolate
-
-
dideazatetrahydrofolate
-
-
dideazatetrahydrofolate hexaglutamate
-
-
homo-dideazatetrahydrofolate
-
-
L-(+)-10-Formyltetrahydrofolate
-
excellent competitive inhibitor
LY231514
-
antitumor activity may result from simultaneous and multiple inhibition of several key folate-requiring enzymes via its polyglutamated metabolites
LY231514 monoglutamate
-
-
LY231514 pentaglutamate
-
-
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
-
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
-
N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
effective inhibitor
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
-
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
-
N-{4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl}-L-glutamic acid
-
-
N-{4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl}-L-glutamic acid
-
-
N-{4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl}-L-glutamic acid
-
-
N10-(Bromoacetyl)-5,8-dideazafolate
-
-
N10-formyl-2,4-diaminodideazafolate
-
-
additional information
-
10-methanesulfonyl-DDACTHF, 10-methanesulfonyl-5-DACTHF and 10-methylthio-DDACTHF shows KI-values above 0.1 mM
-
additional information
-
no inhibition by N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
glycine
-
stimulatory effect
histidine
-
stimulatory effect
NH4+
-
activity enhanced by
tryptophan
-
stimulatory effect
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0775
-
(6R)-N10-formyltetrahydrofolate
-
pH 8.5
0.0848
-
(6R)-N10-formyltetrahydrofolate
-
pH 7.5
7.5e-05
-
10-formyl-5,8-dideazafolate
-
recombinant enzyme
0.00025
-
10-formyl-5,8-dideazafolate
-
-
0.0009
-
10-formyl-5,8-dideazafolate
-
wild type enzyme
0.0013
-
10-formyl-5,8-dideazafolate
-
-
0.0015
-
10-formyl-5,8-dideazafolate
-
recombinant enzyme, expressed in E.coli
0.0015
-
10-formyl-5,8-dideazafolate
-
mutant K170R
0.0019
-
10-formyl-5,8-dideazafolate
-
-
0.01
-
10-formyl-5,8-dideazafolate
-
mutant H108A
0.0123
-
10-formyl-5,8-dideazafolate
-
wild-type
0.0123
-
10-formyl-5,8-dideazafolate
-
-
0.017
-
10-formyl-5,8-dideazafolate
-
-
0.0196
-
10-formyl-5,8-dideazafolate
-
mutant N106Q
0.024
-
10-formyl-5,8-dideazafolate
-
mutant H108Q
0.025
-
10-formyl-5,8-dideazafolate
-
mutant D144A
0.035
-
10-formyl-5,8-dideazafolate
-
purN/purU hybrid enzyme
0.128
-
10-formyl-5,8-dideazafolate
-
mutant 13N + 13C 1:1
0.131
-
10-formyl-5,8-dideazafolate
-
mutant B13
0.9
-
10-formyl-5,8-dideazafolate
-
mutant N106G
0.99
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
4
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212); wild-type enzyme
12
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
21
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
33
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.019
-
5,8-Dideazafolate
-
-
0.00368
-
acetate
-
purT gene product
0.0774
-
ATP
-
purT gene product
0.0011
-
beta-glycinamide ribonucleotide
-
-
0.0011
-
beta-glycinamide ribonucleotide
-
wild type enzyme
0.0018
-
beta-glycinamide ribonucleotide
-
mutant K170R
0.008
-
beta-glycinamide ribonucleotide
-
pH 8.5, tetrahydrofolate as cofactor
0.0101
-
beta-glycinamide ribonucleotide
-
purT gene product
0.0101
-
beta-glycinamide ribonucleotide
-
-
0.012
-
beta-glycinamide ribonucleotide
-
pH 7.5, tetrahydrofolate as cofactor
0.0122
-
beta-glycinamide ribonucleotide
-
pH 7.5, tetrahydrofolate as cofactor
0.016
-
beta-glycinamide ribonucleotide
-
purN/purU hybrid enzyme
0.018
-
beta-glycinamide ribonucleotide
-
mutant H108Q
0.019
-
beta-glycinamide ribonucleotide
-
-
0.0192
-
beta-glycinamide ribonucleotide
-
pH 7.5, dideazafolate as cofactor
0.0235
-
beta-glycinamide ribonucleotide
-
pH 8.5, dideazafolate as cofactor
0.029
-
beta-glycinamide ribonucleotide
-
mutant D144A
0.0295
-
beta-glycinamide ribonucleotide
-
mutant N106Q
0.054
-
beta-glycinamide ribonucleotide
-
mutant H108A
0.095
-
beta-glycinamide ribonucleotide
-
wild-type
0.115
-
beta-glycinamide ribonucleotide
-
strain H108A
0.118
-
beta-glycinamide ribonucleotide
-
wild-type
0.118
-
beta-glycinamide ribonucleotide
-
-
0.122
-
beta-glycinamide ribonucleotide
-
strain D144A
0.178
-
beta-glycinamide ribonucleotide
-
mutant B13
0.19
-
beta-glycinamide ribonucleotide
-
mutant 13N + 13C 1:1
0.414
-
beta-glycinamide ribonucleotide
-
strain H121Q
0.99
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
4
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212); wild-type enzyme
12
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
21
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
33
-
beta-glycinamide ribonucleotide
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0036
-
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.0369
-
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.0465
-
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.018
-
carbocyclic glycinamide ribonucleotide
-
-
0.319
-
formate
-
purT gene product
0.102
-
formyl-beta-glycinamide ribonucleotide
-
-
-
0.01
-
glycinamide ribonucleotide
-
-
0.4
-
glycinamide ribonucleotide
-
-
0.052
-
glycinamide ribotide
-
-
0.00576
-
L-(+)-5,10-methenyltetrahydrofolate
-
-
0.0008
-
L-(-)-10-formyltetrahydrofolate
-
-
0.00594
-
L-(-)-10-formyltetrahydrofolate
-
in absence of trifunctional protein
0.00676
-
L-(-)-10-formyltetrahydrofolate
-
-
0.21
-
N-formylglycinamide ribotide
-
-
0.0211
-
N10-formyl-5,8-dideazafolate
-
strain H108A; wild-type
0.0284
-
N10-formyl-5,8-dideazafolate
-
strain D144A
0.099
-
N10-formyl-5,8-dideazafolate
-
strain H121Q
0.19
-
N10-formyl-5-deazafolate
-
-
0.15
-
N10-Formyl-8-deazafolate
-
-
0.0167
-
N10-formyldideazafolate
-
pH 7.5
0.0365
-
N10-formyldideazafolate
-
pH 8.5
0.6
-
N10-formylfolate
-
-
0.058
-
N5,N10-anhydroformyl tetrahydrofolate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
13.5
-
(6R)-N10-formyltetrahydrofolate
-
pH 7.5
20.7
-
(6R)-N10-formyltetrahydrofolate
-
pH 8.5
0.0019
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212)
0.0059
-
10-formyl-5,8-dideazafolate
-
wild-type enzyme
0.018
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
0.031
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
0.043
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
0.102
-
10-formyl-5,8-dideazafolate
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0034
-
beta-glycinamide ribonucleotide
-
wild-type enzyme
0.0262
-
beta-glycinamide ribonucleotide
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0662
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212)
0.262
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
0.293
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
0.63
-
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
7.5
-
beta-glycinamide ribonucleotide
-
wild type enzyme
7.7
-
beta-glycinamide ribonucleotide
-
mutant K170R
14
-
beta-glycinamide ribonucleotide
-
mutant N106Q
33
-
carbocyclic glycinamide ribonucleotide
-
-
16.1
-
N10-formyl-5,8-dideazafolate
-
pH 7.5
40
-
N10-formyl-5,8-dideazafolate
-
pH 8.5
1.52
-
N10-formyl-5-deazafolate
-
-
0.069
-
N10-Formyl-8-deazafolate
-
-
0.016
-
N10-formyltetrahydrofolate
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00021
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
-
-
0.0014
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
-
0.0012
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
-
0.0011
-
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
-
1.5e-05
-
10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
-
1.5e-05
-
10-CF3CO-DDACTHF
-
26C, pH 7.5
1.5e-05
-
10-CF3CO-DDACTHF
-
-
3e-05
-
10-CF3CO-DDACTHF
-
-
1.4e-05
-
10-formyl-DDACTHF
-
26C, pH 7.5
1.4e-05
-
10-formyl-DDACTHF
-
-
0.00058
-
10-methanesulfonyl-5-DACTHF
-
26C, pH 7.5
0.00023
-
10-methanesulfonyl-DDACTHF
-
26C, pH 7.5
0.00025
-
10-methylthio-DDACTHF
-
26C, pH 7.5
0.00025
-
10-methylthio-DDACTHF
-
-
1.5e-05
-
10-trifluoroacetyl-DDACTHF
-
26C, pH 7.5
0.0019
-
10-trifluoroacetyl-DDACTHF
-
26C, pH 7.5
5.6e-05
-
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
26C, pH 7.5
0.0048
-
4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
26C, pH 7.5
0.0017
-
DDACTHF
-
26C, pH 7.5
0.0017
-
DDACTHF
-
-
0.005
-
DDACTHF
-
26C, pH 7.5
0.0093
-
LY231514 monoglutamate
-
-
0.0011
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
-
0.00085
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
-
0.00018
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
-
6e-05
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
0.0005
-
N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
26C, pH 7.5
6.5e-05
-
LY231514 pentaglutamate
-
-
additional information
-
additional information
-
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid and 4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid show Ki-values above 0.1 mM
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
7e-05
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
-
8e-05
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
-
9e-05
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
-
0.0055
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
-
0.01
-
(S)-2-(4((R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
-
0.0005
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0006
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0007
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0008
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0009
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.1
-
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
1.6e-05
-
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
1.7e-05
-
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
6e-05
-
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0027
-
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0036
-
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.01
-
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.1
-
DDACTHF
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.00852
-
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
pH 7.5, 37C
0.0024
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0044
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.0055
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0057
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.01
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0005
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0006
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.0007
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.006
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
5e-05
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
6e-05
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
7e-05
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.005
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.001
-
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
in situ
0.02
-
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
in vitro
1.8e-05
-
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
in situ
1.4e-05
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in situ
0.0002
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.00078
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro
0.01
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.1
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
6.8e-06
-
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
in situ
0.00015
-
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
in vitro
7.2e-06
-
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
in situ
0.00226
-
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
in vitro
8.6e-06
-
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
in situ
0.00251
-
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
in vitro
3e-05
-
pemetrexed
-
in situ
0.02
-
pemetrexed
-
in vitro
0.00551
-
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
pH 7.5, 37C
additional information
-
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
IC50 value above 0.02 mM, pH 7.5, 37C
additional information
-
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
IC50 value above 0.02 mM, pH 7.5, 37C
additional information
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
value above 0.05
0.00244
-
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
in vitro
additional information
-
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
-
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.651
-
-
-
0.95
-
-
-
1.294
-
-
-
3.3
-
-
-
14
-
-
purN gene product
14.8
-
-
-
53.6
-
-
-
additional information
-
-
purT gene product, 0.052 mg /min.mg.mL
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
in vitro GARFT enzyme inhibition assay
7.8
-
-
Tris buffer, 38C
8
-
-
purTgene product
8.5
8.8
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
11
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
37
-
-
in situ GARFT enzyme inhibition assay
37
-
-
in vitro GARFT enzyme inhibition assay
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
-
xenograft HXGC3, HC1 and VRC5
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
-
murine lymphoma cell line
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
-
xenograft, pancreatic carcinoma
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
Q599X3, Q599X4, Q599X5
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Anaplasma phagocytophilum (strain HZ)
Aquifex aeolicus (strain VF5)
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Geobacillus kaustophilus (strain HTA426)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
21060
-
-
calculated from peptide sequence deduced from the cDNA
21800
-
-
recombinant enzyme, predicted from cDNA
21840
-
-
recombinant enzyme, MALDI-TOF mass spectral analysis
23000
-
-
purN encoded, DNA sequencing
23000
-
-
-
23240
-
-
calculated from peptide sequence deduced from the cDNA
23240
-
-
amino acid sequence, deduced from nucleotide sequence
23240
-
-
-
24000
-
-
equilibrium sedimentation ultracentrifugation
25700
-
-
SDS-PAGE
31000
-
-
DNA sequencing
41900
-
-
product of the purT gene, SDS-PAGE
42000
-
-
product of the purT gene, deduced from predicted amino acid sequence
42400
-
-
product of the purT gene
70000
-
-
gel filtration
100000
-
-
protein possessing transformylase activity, sucrose density gradient ultracentrifugation
100000
-
-
-
108000
-
-
human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme
110000
120000
-
gel filtration, sucrose density gradient ultracentrifugation, sedimentation velocity
110000
-
-
SDS-PAGE
110000
-
-
GAR transformylase, sucrose density gradient ultracentrifugation
110000
-
-
-
112000
-
-
HeLa and L1210 cells
115000
-
-
-
117000
-
-
ultracentrifugation
125000
-
-
sucrose density gradient ultracentrifugation
140000
-
-
sucrose density ultracentrifugation
150000
-
-
gel filtration
160000
-
-
trifunctional protein, sucrose density gradient ultracentrifugation
190000
-
-
trifunctional protein, sucrose density gradient ultracentrifugation
201000
-
-
gel filtration
201000
-
-
-
218000
-
-
gel filtration
218000
-
-
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 30985, calculated
?
P12040
x * 21700, calculated, x * 26000, SDS-PAGE of recombinant protein including His-tag
dimer
-
2 * 63000, transformylase, homodimer, SDS-PAGE; 2 * 83000, trifunctional protein, SDS-PAGE; homodimer
dimer
-
2 * 55000, SDS-PAGE, sucrose density ultracentrifugation, proteolytic cleavage; homodimer
dimer
-
2 * 61000, GAR transformylase, SDS-PAGE; 2 * 95000, trifunctional protein, SDS-PAGE
dimer
-
-
dimer
-
2 * 61000, SDS-PAGE
dimer
-
2 * 55000-57000, SDS-PAGE
dimer
-
1 * 12100 + 1 * 11200, heterodimer, SDS-PAGE
dimer
-
pH-dependent dimer-monomer transition near physiological pH might act as a means of regulating enzyme activity
dimer
-, P71554
dynamic light-scattering and gel filtration
monomer
-
1 * 70000, gel filtration
monomer
-
-
monomer
-
1 + 25000, SDS-PAGE
monomer
-
1 * 117000, ultracentrifugation
monomer
-
1 * 102000, SDS-PAGE; 1 * 110000, SDS-PAGE
monomer
-
1 * 42000, product of the purT gene
monomer
-
1 * 25700, SDS-PAGE
monomer
-
1 * 23000
monomer
Escherichia coli TX635
-
1 * 42000, product of the purT gene
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
diffraction to 2.5 A, space group P3121
P12040
crystals grown at 20C by hanging-drop vapor diffusion, space group C222, a = 140.9 A, b = 97.6 A, c = 102.4
-
crystals grown by vapor diffusion, space group C222, a = 140.5 A, b = 98.2 A, c = 103.3 A
-
orthorhombic space group C222, a = 141.4 A, b = 98.2 A, c = 103.5 A
-
vapor diffusion sitting drop method, crystals of enzyme in complex with 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
in complex with magnesium and iodide at 2.2 A resolution, and with cofactor analogue 5-methyltetrahydrofolate at 2.2 A resolution
-, P71554
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10C, 8months, no appreciable loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant protein
P12040
transformylase, product of the purT gene
-
copurification of trifunctional 5,10-methenyl-, 5,10-methylene, and 10-formylH4folate synthetase, aminoimidazole carboxamide ribotide transformylase and serine transhydroxymethylase
-
partially
-
purified in combination with formyl-methenyl-methylenetetrahydrofolate synthetase
-
recombinant enzyme, expressed in E. coli
-
using a NiHisTrap HP column, the affinity tag is removed by cleavage with TEV protease
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cloning of a 12-gene cluster encoding 9 enzymes for de novo purine nucleotide synthesis
-
expression in Escherichia coli
P12040
cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene; cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene; cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene
Q599X3, Q599X4, Q599X5
coding region of Escherichia coli GART amplified from chromosomal DNA with PCR method
-
harboring expression vector pJS167, encoding the purN gene
-
hybrid glycinamide ribonucleotide transformylase, ribonucleotide binding domain from purN, catalytic machinery and formyl tetrahydrofolate binding domain from purU encoded formyl tetrahydrofolate hydrolase
-
second GAR transformylase encoded by the Escherichia coli purT gene
-
cloned and expressed in Escherichia coli BL21(DE3)pLysS
-
cloned and expressed in Escherichia coli HMS174(DE3)/pCC12
-
cloned by functional complementation in Escherichia coli
-
hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202), GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
-
into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells
-
cDNA, recombinant enzyme expressed in insect cells with a recombinant baculovirus
-
expression in Escherichia coli
-, P71554
; pvdF gene characterization
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D144E
-
catalytically inactive mutant
D144H
-
catalytically inactive mutant
H108K
-
catalytically inactive mutant
H108R
-
catalytically inactive mutant
N106D
-
catalytically inactive mutant
N106H
-
catalytically inactive mutant
K170R
-
catalytically active mutant
additional information
-
hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202),GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
N106Q
-
catalytically active mutant
additional information
-
enzyme disruption mutant, no synthesis of pyoverdine, mutant strain is unable to grow on Kings B agar containing iron-chelating agent EDTA
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs; target for structure-based drug design
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs
medicine
-
glycinamide ribonucleotide transformylase is a validated target for cancer chemotherapy
medicine
-
glycinamide ribonucleotide transformylase has emerged as a productive target for antineoplastic therapeutic intervention
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs
medicine
-
inhibitor 5,10-dideazatetrahydrofolate is antiproliferate and cytotoxic against mammalian tumor cells in culture and have broad spectrum activity against transplantable mouse tumors and human tumor xenografts