Information on EC 2.1.2.2 - phosphoribosylglycinamide formyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.1.2.2
-
RECOMMENDED NAME
GeneOntology No.
phosphoribosylglycinamide formyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
formyl group transfer
-
-
-
-
formyl group transfer
-
-
formyl group transfer
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-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
5-aminoimidazole ribonucleotide biosynthesis I
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-
Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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Metabolic pathways
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One carbon pool by folate
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Purine metabolism
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-
purine metabolism
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-
sulfopterin metabolism
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-
tetrahydrofolate metabolism
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-
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
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-
SYSTEMATIC NAME
IUBMB Comments
10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase
-
-
2-amino-N-ribosylacetamide 5'-phosphate transformylase
-
-
-
-
5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase
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-
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-
GAR formyltransferase
-
-
-
-
GAR formyltransferase
-
-
GAR synthetase
-
-
GAR TFase
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-
-
-
GAR TFase
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-
GAR transformylase
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-
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-
GARFT
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-
GART
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glycinamide ribonucleotide formyltransferase
Q599X3, Q599X4, Q599X5
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glycinamide ribonucleotide formyltransferase
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glycinamide ribonucleotide transformylase
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-
-
-
glycinamide ribonucleotide transformylase
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-
glycinamide ribonucleotide transformylase
-
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glycinamide ribotide formyltransferase
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-
N10-formyltetrahydrolate:2-amino-N-ribosylacetamide-5'-phosphate transformylase
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-
phosphoribosylglycinamide formyltransferase
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-
purN transformylase
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-
CAS REGISTRY NUMBER
COMMENTARY
9032-02-4
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fragment
SwissProt
Manually annotated by BRENDA team
chinese hamster
-
-
Manually annotated by BRENDA team
strain B
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-
Manually annotated by BRENDA team
strain TX635
-
-
Manually annotated by BRENDA team
strains DH5alpha, BL21(DE3)
-
-
Manually annotated by BRENDA team
strains DH5alpha, BL21(DE3), TX680
-
-
Manually annotated by BRENDA team
Escherichia coli TX635
strain TX635
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-
Manually annotated by BRENDA team
; strain PAO1
-
-
Manually annotated by BRENDA team
fission yeast
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
(6R)-N10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
(6R)-tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyl-5,8-dideazafolate + beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
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-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyl-5,8-dideazafolate + N1-(5-phospho-D-ribosyl)glycinamide
?
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + (alpha,beta)-glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
involved in pyoverdine synthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
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-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
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-
r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
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-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
transformylation reaction of purine biosynthesis
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway
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?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
Escherichia coli TX635
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10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide
tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
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-
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10-formyltetrahydrofolate + beta-5-phospho-D-ribosylglycinamide
tetrahydrofolate + beta-5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + carbocyclic beta-glycinamide ribonucleotide
tetrahydrofolate + formyl glycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
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?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
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-
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-
?
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide
tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + N5-hydroxyornithine
tetrahydrofolate + N2-formyl-N5-hydroxyornithine
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + N5-hydroxyornithine
tetrahydrofolate + N5-formyl-N5-hydroxyornithine
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + O-phosphonate carbocyclic glycinamide ribonucleotide
tetrahydrofolate + O-phosphonate-ribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
acetate + ATP
acetyl phosphate + ADP
show the reaction diagram
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
acetate + ATP
acetyl phosphate + ADP
show the reaction diagram
Escherichia coli TX635
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC 2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
beta-glycinamide ribonucleotide + 10-formyltetrahydrofolate
?
show the reaction diagram
-
-
-
-
?
beta-glycinamide ribonucleotide + ATP + formate
beta-formylglycinamide ribonucleotide + ADP
show the reaction diagram
-
assay at 25C
-
-
?
formate + ATP + beta-glycinamide ribonucleotide
beta-formylglycinamide ribonucleotide + ADP
show the reaction diagram
Escherichia coli, Escherichia coli TX635
-
novel non-folate glycinamide ribonucleotide transformylase purT gene product, no significant homology to known EC2.1.2.2 but significant homology to the purK protein also involved in purine biosynthesis
-
-
?
N10-formyl-5-deazafolate + glycinamide ribonucleotide
formylglycinamide ribonucleotide + 5-deazafolate
show the reaction diagram
-
-
-
-
?
N10-formyl-8-deazafolate + glycinamide ribonucleotide
formylglycinamide ribonucleotide + 8-deazafolate
show the reaction diagram
-
-
-
-
?
N10-formyldideazafolate + 5-phospho-D-ribosylglycinamide
dideazafolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
N10-formylfolate + glycinamide ribonucleotide
folate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
N5,N10-anhydroformyl-5,6,7,8tetrahydrofolic acid + 2-amino-N-ribosylacetamide 5'-phosphate + H2O
formylglycinamide ribotide + tetrahydrofolic acid + 2-formamido-N-ribosylacetamide 5'-phosphate
show the reaction diagram
-
-
-
-
ir
N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O
N10-formyltetrahydrofolate + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
-
N5,N10-anhydroformyltetrahydrofolate + glycinamide ribotide + H2O
N10-formyltetrahydrofolate + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
?
N5,N10-anhydroformyltetrahydrofolic acid + glycinamide ribonucleotide
formylglycinamide ribonucleotide + ?
show the reaction diagram
-
-
-
-
?
inosinic acid + glycinamide ribotide + H2O
5-amino-4-imidazolecarboxamide ribotide + formylglycinamide ribotide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
-
-
-
-
additional information
?
-
-
monofunctional enzyme
-
-
-
additional information
?
-
-
monofunctional enzyme
-
-
-
additional information
?
-
-
reaction does not occur if glycine, glycinamide or glycinamide riboside is substituted for glycinamide ribotide
-
-
-
additional information
?
-
-
glycinamide ribonucleotide nucleoside is no substrate
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
trifunctional enzyme, GAR synthetase, aminoimidazole ribonucleotide synthetase and GAR transformylase
-
-
-
additional information
?
-
-
alpha-glycinamide ribonucleotide and glycinamide ribonucleotide nucleoside are neither substrates for no inhibitors
-
-
-
additional information
?
-
-
10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
3 activities in a multifunctional protein, posessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotidetransformylase and aminoimidazole ribonucleotide synthetase
-
-
-
additional information
?
-
-
purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels
-
-
-
additional information
?
-
-
GAR transformylase activity copurifies with 5,10-methylene-, 5,10-methenyl, and 10-formyl-H4folate sythetase (combined) trifunctional enzyme, EC 6.3.4.3, EC 1.5.1.5 and EC 3.5.4.9
-
-
-
additional information
?
-
-
purified along with 10-formyltetrahydrofolate synthetase, uses both diastereomers of 5,10-methenyltetrahydrofolate, (+)-L-diastereomer is used 120 times faster than the (-)-L-diastereomer
-
-
-
additional information
?
-
Escherichia coli TX635
-
10-formyl-dideazaformate, 5-formyltetrahydroformate, formylaminoimidazolecarboxamide ribonucleotide, formylmethionine, formiminoglutamate, GTP, CTP, TTP, ITP and ZTP are no substrates for the purT GAR transformylase, purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
involved in pyoverdine synthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
third step in de novo purine biosynthetic pathway
-
-
r
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
-
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
transformylation reaction of purine biosynthesis
-
-
?
10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
show the reaction diagram
-
carries out the second, third and fifth sequential reactions of de novo purine synthesis pathway
-
-
?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
10-formyltetrahydrofolate + glycinamide ribonucleotide
tetrahydrofolate + formylglycinamide ribonucleotide
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
10-formyl-5,8-dideazafolate
-
-
10-formyl-5,8-dideazafolate
-
-
5,10-methenyltetrahydrofolate
-
-
folate
-
-
formate
-
second GAR transformylase using formate and ATP in place of 10-formyltetrahydrofolate
L-(+-)-5,10-methenyltetrahydrofolate
-
-
L-(-)-10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
only formyldonor
N10-formyltetrahydrofolate
-
-
N10-formyltetrahydrofolate
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
-
expression regulated by amount of iron in the growth medium
K+
-
activity enhanced by
Mg2+
-
purT gene product
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(6R)-5,10-dideazatetrahydrofolate
-
lometrexol
(6R)-dideazatetrahydrofolate diglutamate
-
LY235340
(6R)-dideazatetrahydrofolate pentaglutamate
-
LY235542
(6R)-dideazatetrahydrofolate tetraglutamate
-
LY266978
(6R)-dideazatetrahydrofolate triglutamate
-
LY235337
(6S)-dideazatetrahydrofolate
-
LY243246
(6S)-N10-formyltetrahydrofolate
-
-
(alpha,beta)-hydroxyacetamide ribonucleotide
-
-
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
-
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
-
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
-
10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
specifically inhibits recombinant GAR Tfase, inactive against other folate-dependent enzymes, potent inhibitor of tumor cell proliferation
10-Acetyl-5,8-dideazafolate
-
-
10-Acetyl-5,8-dideazafolate
-
-
10-CF3CO-DDACTHF
-
-
10-formyl-5,8-dideazafolic acid hexaglutamate
-
-
10-formyl-DDACTHF
-
-
10-methanesulfonyl-5-DACTHF
-
-
10-methanesulfonyl-DDACTHF
-
-
10-methylthio-DDACTHF
-
-
10-trifluoroacetyl-DDACTHF
-
-
2',3'-dideoxy-2',3'-didehydro-carbocyclic glycinamide ribonucleotide
-
-
2',3'-dideoxy-carbocyclic glycinamide ribonucleotide
-
-
2',5'-furan-dideazatetrahydrofolate
-
LY222306
2',5'-thiophene dideazatetrahydrofolate
-
LY309887, diastereomer B
2',5'-thiophene dideazatetrahydrofolate
-
LY309886, diastereomer A; LY309887, diastereomer B
2-deaminodideazatetrahydrofolate
-
-
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
-
4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
-
5'-phosphonate carbocyclic glycinamide ribonucleotide
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolate
-
-
5,10-dideaza-5,6,7,8-tetrahydrofolic acid
-
-
5,10-dideazafolic acid
-
-
5,10-dideazatetrahydrofolate
-
-
5,10-dideazatetrahydrofolate
-
-
5,10-dideazatetrahydrofolate
-
-
5,8-Dideazafolate
-
-
5,8-dideazafolate hexaglutamate
-
-
5,8-dideazapteroyl hexaglutamate
-
-
5-deazatetrahydrofolate
-
-
AG2034
-
-
AG2037
-
-
alpha,beta-hydroxyacetamideribonucleotide
-
-
alpha,beta-N-(hydroxyacetyl)-D-ribofuranosylamine
-
-
beta-thioglycinamide dideazafolate
-
-
dideazafolate
-
-
dideazatetrahydrofolate
-
-
dideazatetrahydrofolate hexaglutamate
-
-
homo-dideazatetrahydrofolate
-
-
L-(+)-10-Formyltetrahydrofolate
-
excellent competitive inhibitor
LY231514
-
antitumor activity may result from simultaneous and multiple inhibition of several key folate-requiring enzymes via its polyglutamated metabolites
LY231514 monoglutamate
-
-
LY231514 pentaglutamate
-
-
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamylglutamic acid
-
-
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
-
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
-
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
-
N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
effective inhibitor
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
-
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
-
N-{4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl}-L-glutamic acid
-
-
N-{4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl}-L-glutamic acid
-
-
N-{4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl}-L-glutamic acid
-
-
N10-(Bromoacetyl)-5,8-dideazafolate
-
-
N10-formyl-2,4-diaminodideazafolate
-
-
LY309887
-
-
additional information
-
10-methanesulfonyl-DDACTHF, 10-methanesulfonyl-5-DACTHF and 10-methylthio-DDACTHF shows KI-values above 0.1 mM
-
additional information
-
no inhibition by N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
glycine
-
stimulatory effect
histidine
-
stimulatory effect
NH4+
-
activity enhanced by
tryptophan
-
stimulatory effect
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0775
(6R)-N10-formyltetrahydrofolate
-
pH 8.5
0.0848
(6R)-N10-formyltetrahydrofolate
-
pH 7.5
0.000075
10-formyl-5,8-dideazafolate
-
recombinant enzyme
0.00025
10-formyl-5,8-dideazafolate
-
-
0.0009
10-formyl-5,8-dideazafolate
-
wild type enzyme
0.0013
10-formyl-5,8-dideazafolate
-
-
0.0015
10-formyl-5,8-dideazafolate
-
recombinant enzyme, expressed in E.coli
0.0015
10-formyl-5,8-dideazafolate
-
mutant K170R
0.0019
10-formyl-5,8-dideazafolate
-
-
0.01
10-formyl-5,8-dideazafolate
-
mutant H108A
0.0123
10-formyl-5,8-dideazafolate
-
wild-type
0.0123
10-formyl-5,8-dideazafolate
-
-
0.017
10-formyl-5,8-dideazafolate
-
-
0.0196
10-formyl-5,8-dideazafolate
-
mutant N106Q
0.024
10-formyl-5,8-dideazafolate
-
mutant H108Q
0.025
10-formyl-5,8-dideazafolate
-
mutant D144A
0.035
10-formyl-5,8-dideazafolate
-
purN/purU hybrid enzyme
0.128
10-formyl-5,8-dideazafolate
-
mutant 13N + 13C 1:1
0.131
10-formyl-5,8-dideazafolate
-
mutant B13
0.9
10-formyl-5,8-dideazafolate
-
mutant N106G
0.99
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
4
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212); wild-type enzyme
12
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
21
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
33
10-formyl-5,8-dideazafolate
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.019
5,8-Dideazafolate
-
-
0.00368
acetate
-
purT gene product
0.0774
ATP
-
purT gene product
0.0011
beta-glycinamide ribonucleotide
-
-
0.0011
beta-glycinamide ribonucleotide
-
wild type enzyme
0.0018
beta-glycinamide ribonucleotide
-
mutant K170R
0.008
beta-glycinamide ribonucleotide
-
pH 8.5, tetrahydrofolate as cofactor
0.0101
beta-glycinamide ribonucleotide
-
purT gene product
0.0101
beta-glycinamide ribonucleotide
-
-
0.012
beta-glycinamide ribonucleotide
-
pH 7.5, tetrahydrofolate as cofactor
0.0122
beta-glycinamide ribonucleotide
-
pH 7.5, tetrahydrofolate as cofactor
0.016
beta-glycinamide ribonucleotide
-
purN/purU hybrid enzyme
0.018
beta-glycinamide ribonucleotide
-
mutant H108Q
0.019
beta-glycinamide ribonucleotide
-
-
0.0192
beta-glycinamide ribonucleotide
-
pH 7.5, dideazafolate as cofactor
0.0235
beta-glycinamide ribonucleotide
-
pH 8.5, dideazafolate as cofactor
0.029
beta-glycinamide ribonucleotide
-
mutant D144A
0.0295
beta-glycinamide ribonucleotide
-
mutant N106Q
0.054
beta-glycinamide ribonucleotide
-
mutant H108A
0.095
beta-glycinamide ribonucleotide
-
wild-type
0.115
beta-glycinamide ribonucleotide
-
strain H108A
0.118
beta-glycinamide ribonucleotide
-
wild-type
0.118
beta-glycinamide ribonucleotide
-
-
0.122
beta-glycinamide ribonucleotide
-
strain D144A
0.178
beta-glycinamide ribonucleotide
-
mutant B13
0.19
beta-glycinamide ribonucleotide
-
mutant 13N + 13C 1:1
0.414
beta-glycinamide ribonucleotide
-
strain H121Q
0.99
beta-glycinamide ribonucleotide
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
4
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212); wild-type enzyme
12
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
21
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
33
beta-glycinamide ribonucleotide
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0036
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.0369
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.0465
carbocyclic beta-glycinamide ribonucleotide
-
-
-
0.018
carbocyclic glycinamide ribonucleotide
-
-
0.319
formate
-
purT gene product
0.102
formyl-beta-glycinamide ribonucleotide
-
-
-
0.01
glycinamide ribonucleotide
-
-
0.4
glycinamide ribonucleotide
-
-
0.052
glycinamide ribotide
-
-
0.00576
L-(+)-5,10-methenyltetrahydrofolate
-
-
0.0008
L-(-)-10-formyltetrahydrofolate
-
-
0.00594
L-(-)-10-formyltetrahydrofolate
-
in absence of trifunctional protein
0.00676
L-(-)-10-formyltetrahydrofolate
-
-
0.21
N-formylglycinamide ribotide
-
-
0.0211
N10-formyl-5,8-dideazafolate
-
strain H108A; wild-type
0.0284
N10-formyl-5,8-dideazafolate
-
strain D144A
0.099
N10-formyl-5,8-dideazafolate
-
strain H121Q
0.19
N10-formyl-5-deazafolate
-
-
0.15
N10-Formyl-8-deazafolate
-
-
0.0167
N10-formyldideazafolate
-
pH 7.5
0.0365
N10-formyldideazafolate
-
pH 8.5
0.6
N10-formylfolate
-
-
0.058
N5,N10-anhydroformyl tetrahydrofolate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
13.5
(6R)-N10-formyltetrahydrofolate
-
pH 7.5
20.7
(6R)-N10-formyltetrahydrofolate
-
pH 8.5
0.0019
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212)
0.0059
10-formyl-5,8-dideazafolate
-
wild-type enzyme
0.018
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
0.031
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
0.043
10-formyl-5,8-dideazafolate
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
0.102
10-formyl-5,8-dideazafolate
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0034
beta-glycinamide ribonucleotide
-
wild-type enzyme
0.0262
beta-glycinamide ribonucleotide
-
chimeric enzyme PGX-B12 (PurN1-101/hGART102-202)
0.0662
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M1 (hGART1-100/PurN101-212)
0.262
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M24 (hGART1-54/PurN55-212)
0.293
beta-glycinamide ribonucleotide
-
chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
0.63
beta-glycinamide ribonucleotide
-
chimeric enzyme GPX-M55 (hGART1-56/PurN58-212)
7.5
beta-glycinamide ribonucleotide
-
wild type enzyme
7.7
beta-glycinamide ribonucleotide
-
mutant K170R
14
beta-glycinamide ribonucleotide
-
mutant N106Q
33
carbocyclic glycinamide ribonucleotide
-
-
16.1
N10-formyl-5,8-dideazafolate
-
pH 7.5
40
N10-formyl-5,8-dideazafolate
-
pH 8.5
1.52
N10-formyl-5-deazafolate
-
-
0.069
N10-Formyl-8-deazafolate
-
-
0.016
N10-formylfolate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00021
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
-
0.0014
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
-
0.0012
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
-
0.0011
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
-
0.000015
10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
-
0.000015
10-CF3CO-DDACTHF
-
26C, pH 7.5
0.000015
10-CF3CO-DDACTHF
-
-
0.00003
10-CF3CO-DDACTHF
-
-
0.000014
10-formyl-DDACTHF
-
26C, pH 7.5
0.000014
10-formyl-DDACTHF
-
-
0.00058
10-methanesulfonyl-5-DACTHF
-
26C, pH 7.5
0.00023
10-methanesulfonyl-DDACTHF
-
26C, pH 7.5
0.00025
10-methylthio-DDACTHF
-
26C, pH 7.5
0.00025
10-methylthio-DDACTHF
-
-
0.000015
10-trifluoroacetyl-DDACTHF
-
26C, pH 7.5
0.0019
10-trifluoroacetyl-DDACTHF
-
26C, pH 7.5
0.000056
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
26C, pH 7.5
0.0048
4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid
-
26C, pH 7.5
0.0017
DDACTHF
-
26C, pH 7.5
0.0017
DDACTHF
-
-
0.005
DDACTHF
-
26C, pH 7.5
0.0093
LY231514 monoglutamate
-
-
0.0011
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
-
0.00085
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
-
0.00018
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
-
0.00006
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
-
0.0005
N-[4-[5-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-2-(2,2,2-trifluoroacetyl)pentyl]benzoyl]-L-glutamic acid
-
26C, pH 7.5
0.000065
LY231514 pentaglutamate
-
-
additional information
additional information
-
4-carbamoyl-2-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid and 4-carbamoyl-4-[4-[4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(2,2,2-trifluoroacetyl)butyl]benzoylamino]butyric acid show Ki-values above 0.1 mM
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00007
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.00008
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.00009
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0055
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
(S)-2-(((4R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylthio)butyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.0005
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0006
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0007
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0008
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0009
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
(S)-2-(4((S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.1
(S)-2-(4-(2-(3-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)propyl)-1,3-dithian-2-yl)benzamido)pentanedioic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.000016
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.000017
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.00006
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
10-CF3CO-DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0027
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0036
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.01
DDACTHF
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.1
DDACTHF
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.00852
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acid
-
pH 7.5, 37C
0.0024
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0044
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.0055
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0057
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.01
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-hydroxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.0005
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.0006
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.0007
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.006
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
N-([4-[(1R)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-methoxybutyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.00005
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.00006
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.00007
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine
0.005
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.01
N-([4-[(1S)-4-(2,4-diamino-6-oxo-1,6-dihydropyrimidin-5-yl)-1-(methylsulfanyl)butyl]phenyl]carbonyl)glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine
0.001
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
in situ
0.02
N-([4-[(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)methyl]phenyl]carbonyl)glutamic acid
-
in vitro
0.000018
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
in situ
0.000014
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in situ
0.0002
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity, CCRF-CEM cell, in the presence of thymidine, in the absence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM cell, in the absence of thymidine, in the absence of hypoxanthine
0.00078
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro
0.01
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity, CCRF-CEM cell, in the absence of thymidine, in the presence of hypoxanthine; in vitro cytotoxic activity in mutant cell line, CCRF-CEM/FPGS- cell, in the absence of thymidine, in the absence of hypoxanthine
0.1
N-[4-[2-[(6R)-2-amino-3,4,5,6,7,8-hexahydro-4-oxopyrido[2,3-d]pyrimidin-6-yl]ethyl]benzoyl]-L-glutamic acid
-
in vitro cytotoxic activity in mutant cell line, CCRF-CEM/MTX cell, in the absence of thymidine, in the absence of hypoxanthine
0.0000068
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
in situ
0.00015
N-[4-[4-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)butyl]benzoyl]-L-glutamic acid
-
in vitro
0.0000072
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
in situ
0.00226
N-[4-[5-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)pentyl]benzoyl]-L-glutamic acid
-
in vitro
0.0000086
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
in situ
0.00251
N-[4-[6-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)hexyl]benzoyl]-L-glutamic acid
-
in vitro
0.00003
pemetrexed
-
in situ
0.02
pemetrexed
-
in vitro
0.00551
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acid
-
pH 7.5, 37C
additional information
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acid
-
IC50 value above 0.02 mM, pH 7.5, 37C
additional information
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acid
-
IC50 value above 0.02 mM, pH 7.5, 37C
additional information
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acid
-
value above 0.05
0.00244
N-([4-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)glutamic acid
-
in vitro
additional information
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acid
-
value above 0.05
additional information
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
additional information
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acid
-
value above 0.05
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.651
-
-
1.294
-
-
14
-
purN gene product
additional information
-
purT gene product, 0.052 mg /min.mg.mL
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
-
in vitro GARFT enzyme inhibition assay
7.8
-
Tris buffer, 38C
8
-
purTgene product
8.5 - 8.8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 11
-
-
6 - 9.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
37
-
in situ GARFT enzyme inhibition assay
37
-
in vitro GARFT enzyme inhibition assay
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Anaplasma phagocytophilum (strain HZ)
Aquifex aeolicus (strain VF5)
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Geobacillus kaustophilus (strain HTA426)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
21060
-
calculated from peptide sequence deduced from the cDNA
485718
21800
-
recombinant enzyme, predicted from cDNA
485734
21840
-
recombinant enzyme, MALDI-TOF mass spectral analysis
485734
23000
-
-
485715, 485720
23000
-
purN encoded, DNA sequencing
485733
23000
-
-
485741
23240
-
calculated from peptide sequence deduced from the cDNA
485718
23240
-
amino acid sequence, deduced from nucleotide sequence
485719
23240
-
-
485729
24000
-
equilibrium sedimentation ultracentrifugation
485718
25700
-
SDS-PAGE
485734
31000
-
DNA sequencing
485736
41900
-
product of the purT gene, SDS-PAGE
485732
42000
-
product of the purT gene, deduced from predicted amino acid sequence
485732
42400
-
product of the purT gene
485732
70000
-
gel filtration
485711
100000
-
protein possessing transformylase activity, sucrose density gradient ultracentrifugation
485716
100000
-
-
485719
108000
-
human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme
685128
110000 - 120000
-
gel filtration, sucrose density gradient ultracentrifugation, sedimentation velocity
485728
110000
-
SDS-PAGE
485721, 485722
110000
-
GAR transformylase, sucrose density gradient ultracentrifugation
485723
110000
-
-
485725
112000
-
HeLa and L1210 cells
485725
115000
-
-
485725
117000
-
ultracentrifugation
485721
125000
-
sucrose density gradient ultracentrifugation
485724
140000
-
sucrose density ultracentrifugation
485721
145000
-
-
485718
150000
-
gel filtration
485716
150000
-
-
485723
160000
-
trifunctional protein, sucrose density gradient ultracentrifugation
485716
190000
-
trifunctional protein, sucrose density gradient ultracentrifugation
485723
201000
-
gel filtration
485716
201000
-
-
485723
215000
-
-
485723
218000
-
gel filtration
485716
218000
-
-
485723
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 30985, calculated
?
P12040
x * 21700, calculated, x * 26000, SDS-PAGE of recombinant protein including His-tag
dimer
-
-
dimer
-
homodimer
dimer
-
2 * 61000, SDS-PAGE
dimer
-
1 * 12100 + 1 * 11200, heterodimer, SDS-PAGE
dimer
-
pH-dependent dimer-monomer transition near physiological pH might act as a means of regulating enzyme activity
dimer
-
2 * 63000, transformylase, homodimer, SDS-PAGE
dimer
-
2 * 61000, GAR transformylase, SDS-PAGE
dimer
-
2 * 55000, SDS-PAGE, sucrose density ultracentrifugation, proteolytic cleavage
dimer
-
2 * 55000-57000, SDS-PAGE
dimer
-
2 * 83000, trifunctional protein, SDS-PAGE
dimer
-
2 * 95000, trifunctional protein, SDS-PAGE
dimer
P9WHM5
dynamic light-scattering and gel filtration
monomer
-
-
monomer
-
1 * 110000, SDS-PAGE, 1 * 102000, SDS-PAGE
monomer
-
1 * 70000, gel filtration
monomer
-
1 * 42000, product of the purT gene
monomer
-
1 * 23000
monomer
-
1 * 117000, ultracentrifugation
monomer
-
1 * 25700, SDS-PAGE
monomer
-
1 + 25000, SDS-PAGE
monomer
Escherichia coli TX635
-
1 * 42000, product of the purT gene
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
diffraction to 2.5 A, space group P3121
P12040
crystals grown at 20C by hanging-drop vapor diffusion, space group C222, a = 140.9 A, b = 97.6 A, c = 102.4
-
crystals grown by vapor diffusion, space group C222, a = 140.5 A, b = 98.2 A, c = 103.3 A
-
orthorhombic space group C222, a = 141.4 A, b = 98.2 A, c = 103.5 A
-
vapor diffusion sitting drop method, crystals of enzyme in complex with 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid
P22102
in complex with magnesium and iodide at 2.2 A resolution, and with cofactor analogue 5-methyltetrahydrofolate at 2.2 A resolution
P9WHM5
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml of 22 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M lithium chloride, 0.1 M sodium cacodylate trihydrate, pH 6.6, 14% PEG 3350, and equilibration against 0.2 ml of reservoir solution, 18C, method optimization, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
-
-
485741
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, 8months, no appreciable loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein
P12040
transformylase, product of the purT gene
-
copurification of trifunctional 5,10-methenyl-, 5,10-methylene, and 10-formylH4folate synthetase, aminoimidazole carboxamide ribotide transformylase and serine transhydroxymethylase
-
partially
-
purified in combination with formyl-methenyl-methylenetetrahydrofolate synthetase
-
recombinant enzyme, expressed in E. coli
-
using a NiHisTrap HP column, the affinity tag is removed by cleavage with TEV protease
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography, and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of a 12-gene cluster encoding 9 enzymes for de novo purine nucleotide synthesis
-
expression in Escherichia coli
P12040
cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene; cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene; cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene
Q599X3, Q599X4, Q599X5
coding region of Escherichia coli GART amplified from chromosomal DNA with PCR method
-
harboring expression vector pJS167, encoding the purN gene
-
hybrid glycinamide ribonucleotide transformylase, ribonucleotide binding domain from purN, catalytic machinery and formyl tetrahydrofolate binding domain from purU encoded formyl tetrahydrofolate hydrolase
-
second GAR transformylase encoded by the Escherichia coli purT gene
-
cloned and expressed in Escherichia coli BL21(DE3)pLysS
-
cloned and expressed in Escherichia coli HMS174(DE3)/pCC12
-
cloned by functional complementation in Escherichia coli
-
hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202), GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
-
into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells
-
cDNA, recombinant enzyme expressed in insect cells with a recombinant baculovirus
-
expression in Escherichia coli
P9WHM5
; pvdF gene characterization
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D144E
-
catalytically inactive mutant
D144H
-
catalytically inactive mutant
H108K
-
catalytically inactive mutant
H108R
-
catalytically inactive mutant
N106D
-
catalytically inactive mutant
N106H
-
catalytically inactive mutant
K170R
-
catalytically active mutant
additional information
-
hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202),GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
N106Q
-
catalytically active mutant
additional information
-
enzyme disruption mutant, no synthesis of pyoverdine, mutant strain is unable to grow on Kings B agar containing iron-chelating agent EDTA
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
target for structure-based drug design
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs
medicine
-
glycinamide ribonucleotide transformylase has emerged as a productive target for antineoplastic therapeutic intervention
medicine
-
glycinamide ribonucleotide transformylase is a validated target for cancer chemotherapy
medicine
-
inhibitor 5,10-dideazatetrahydrofolate is antiproliferate and cytotoxic against mammalian tumor cells in culture and have broad spectrum activity against transplantable mouse tumors and human tumor xenografts
medicine
-
pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs