Information on EC 2.1.1.88 - 8-hydroxyquercetin 8-O-methyltransferase

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The expected taxonomic range for this enzyme is: Gunneridae

SplaateEC_Number,Commentary
EC NUMBER
COMMENTARY
2.1.1.88
-
SplaateRecommended_Name,GO_Number
RECOMMENDED NAME
GeneOntology No.
8-hydroxyquercetin 8-O-methyltransferase
-
SplaateReaction,Reaction_id,Commentary,IF(Commentary != '',Organism,'') ,IF(Commentary != '',Literature,'')
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone
show the reaction diagram
mechanism
-
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
-
SplaateReaction_Type,Organism,Commentary,Literature
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
SplaatePathway,BRENDA_Link,KEGG_Link,MetaCyc_Link,Source_Database
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gossypetin metabolism
-
-
phenylpropanoids methylation (ice plant)
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SplaateSystematic_Name,Commentary_IUBMB
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3,5,7,8,3',4'-hexahydroxyflavone 8-O-methyltransferase
Also acts on 8-hydroxykaempferol, but not on the glycosides of 8-hydroxyflavonols. An enzyme from the flower buds of Lotus corniculatus.
SplaateSynonyms,Organism,Commentary,Literature
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
flavonol 8-O-methyltransferase
-
-
-
-
methyltransferase, flavonol 8-
-
-
-
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SplaateCAS_Registry_Number,Commentary
CAS REGISTRY NUMBER
COMMENTARY
99775-17-4
-
SplaateOrganism, Commentary,Literature, Sequence_Code,Sequence_db,Textmining
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SplaateGeneral_Information, Organism, Commentary, Literature
SplaateSubstrates,Products,id,Organism_Substrates,Commentary_Substrates, Literature_Substrates, Commentary_Products, Literature_Products,Reversibility
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
i.e. 8-hydroxyquercetin
i.e. 8-methoxyquercetin
?
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
additional information
?
-
-
not: glycosides of 8-hydroxyflavonols
-
-
-
SplaateNatural_Substrates,Natural_Products,id,Organism_Substrates,Commentary_Substrates,Literature_Substrates,Commentary_Products,Literature_Products,Reversibility
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
SplaateCofactor,Organism,Commentary,Literature,Filename
SplaateMetals_Ions,Organism,Commentary, Literature
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
required
SplaateInhibitors, Organism, Commentary, Literature,Filename
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8-Methoxykaempferol
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product inhibition, competitive with respect to 8-hydroxykaempferol
EDTA
-
in absence of Mg2+
S-adenosyl-L-homocysteine
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product inhibition, non-competitive with respect to S-adenosyl-L-methionine
SplaateActivating_Compound, Organism, Commentary, Literature,Filename
SplaateKM_Value,KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0013
8-Hydroxykaempferol
-
-
0.053
S-adenosyl-L-methionine
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-
SplaateTurnover_Number, Turnover_Number_Maximum, Substrate,Organism,Commentary, Literature, Filename
SplaateKCat_KM_Value,KCat_KM_Value_Maximum, Substrate,Organism, Commentary, Literature, Filename
SplaateKI_Value,KI_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateIC50_Value,IC50_Value_Maximum, Inhibitor,Organism, Commentary, Literature, Filename
SplaateSpecific_Activity, Specific_Activity_Maximum, Organism ,Commentary, Literature
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
-
SplaatepH_Optimum, pH_Optimum_Maximum, Organism, Commentary, Literature
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SplaatepH_Range,pH_Range_Maximum, Organism,Commentary, Literature
SplaateTemperature_Optimum, Temperature_Optimum_Maximum, Organism, Commentary, Literature
SplaateTemperature_Range, Temperature_Range_Maximum, Organism, Commentary, Literature
SplaatepI_Value,pI_Value_Maximum, Organism,Commentary, Literature
SplaateSource_Tissue, Organism, Commentary, Literature, Textmining
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
SplaateLocalization, Organism, Commentary, id_go, Literature, Textmining
SplaatePDB,PDB,PDB,Organism,Uniprot_ID
SplaateMolecular_Weight, Molecular_Weight_Maximum, Organism, Commentary, Literature
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55000
-
gel filtration
485658
SplaateSubunits, Organism, Commentary, Literature
SplaatePosttranslational_Modification, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaatepH_Stability,pH_Stability_Maximum, Organism, Commentary, Literature
SplaateTemperature_Stability,Temperature_Stability_Maximum, Organism, Commentary, Literature
SplaateGeneral_Stability, Organism, Literature
SplaateOrganic_Solvent, Organism, Commentary, Literature
SplaateOxidation_Stability,Organism,Literature
SplaateStorage_Stability, Organism, Literature
SplaateCommentary, Organism, Literature
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
SplaateCommentary, Organism, Literature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
SplaateCommentary, Organism, Literature
SplaateEngineering, Organism, Commentary, Literature
SplaateCommentary, Organism, Literature
SplaateApplication,Organism,Commentary,Literature