Information on EC 2.1.1.88 - 8-hydroxyquercetin 8-O-methyltransferase

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The expected taxonomic range for this enzyme is: Gunneridae

EC NUMBER
COMMENTARY
2.1.1.88
-
RECOMMENDED NAME
GeneOntology No.
8-hydroxyquercetin 8-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone
show the reaction diagram
mechanism
-
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3,5,7,8,3',4'-hexahydroxyflavone 8-O-methyltransferase
Also acts on 8-hydroxykaempferol, but not on the glycosides of 8-hydroxyflavonols. An enzyme from the flower buds of Lotus corniculatus.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
flavonol 8-O-methyltransferase
-
-
-
-
methyltransferase, flavonol 8-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
99775-17-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
i.e. 8-hydroxyquercetin
i.e. 8-methoxyquercetin
?
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
additional information
?
-
-
not: glycosides of 8-hydroxyflavonols
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8-Methoxykaempferol
-
product inhibition, competitive with respect to 8-hydroxykaempferol
EDTA
-
in absence of Mg2+
S-adenosyl-L-homocysteine
-
product inhibition, non-competitive with respect to S-adenosyl-L-methionine
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0013
-
8-Hydroxykaempferol
-
-
0.053
-
S-adenosyl-L-methionine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55000
-
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
Q6VMW0