Information on EC 2.1.1.84 - methylquercetagetin 6-O-methyltransferase

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The expected taxonomic range for this enzyme is: Chrysosplenium americanum

EC NUMBER
COMMENTARY
2.1.1.84
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RECOMMENDED NAME
GeneOntology No.
methylquercetagetin 6-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-adenosyl-L-methionine + 5,6,3',4'-tetrahydroxy-3,7-dimethoxyflavone = S-adenosyl-L-homocysteine + 5,3',4'-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
mechanism
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S-adenosyl-L-methionine + 5,6,3',4'-tetrahydroxy-3,7-dimethoxyflavone = S-adenosyl-L-homocysteine + 5,3',4'-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
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-
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PATHWAY
KEGG Link
MetaCyc Link
polymethylated quercetin glucoside biosynthesis II - quercetagetin series (Chrysosplenium)
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superpathway polymethylated quercetin/quercetagetin glucoside biosynthesis (Chrysosplenium)
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5,6,3',4'-tetrahydroxy-3,7-dimethoxyflavone 6-O-methyltransferase
The enzymes from Chrysosplenium americanum also methylates 3,7,3'-trimethylquercetagetin at the 6-position. Does not act on flavones, dihydroflavonols, or their glucosides.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-OMT
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-
-
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flavonol 6-O-methyltransferase
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-
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methyltransferase, flavonol 6-
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-
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CAS REGISTRY NUMBER
COMMENTARY
96477-61-1
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine
3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine
show the reaction diagram
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-
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3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine
3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine
show the reaction diagram
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-
?
8-hydroxykaempferol + S-adenosyl-L-methionine
?
show the reaction diagram
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25% of activity with 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
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-
?
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3,6,7,3'-Tetramethylquercetagetin
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noncompetitive product inhibition
p-chloromercuribenzoate
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S-adenosyl-L-homocysteine
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competitive product inhibition
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.018
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3,7,3'-Trimethylquercetagetin
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0.051
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S-adenosyl-L-methionine
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SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
57000
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-15°C, 0.2 M potassium phosphate, pH 8, 20% glycerol, 5 mM dithiothreitol, for several months, no loss of activity
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4°C, 4-6 weeks, 80% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ammonium sulfate, Sephacryl S-200, hydroxylapatite, chromatofocusing
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chromatofocusing on Superose 12 and Mono P
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