Information on EC 2.1.1.84 - methylquercetagetin 6-O-methyltransferase

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The expected taxonomic range for this enzyme is: Chrysosplenium americanum

EC NUMBER
COMMENTARY
2.1.1.84
-
RECOMMENDED NAME
GeneOntology No.
methylquercetagetin 6-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 5,6,3',4'-tetrahydroxy-3,7-dimethoxyflavone = S-adenosyl-L-homocysteine + 5,3',4'-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
polymethylated quercetin glucoside biosynthesis II - quercetagetin series (Chrysosplenium)
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superpathway polymethylated quercetin/quercetagetin glucoside biosynthesis (Chrysosplenium)
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5,6,3',4'-tetrahydroxy-3,7-dimethoxyflavone 6-O-methyltransferase
The enzymes from Chrysosplenium americanum also methylates 3,7,3'-trimethylquercetagetin at the 6-position. Does not act on flavones, dihydroflavonols, or their glucosides.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6-OMT
-
-
-
-
flavonol 6-O-methyltransferase
-
-
-
-
methyltransferase, flavonol 6-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
96477-61-1
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine
3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
-
-
3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine
3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
8-hydroxykaempferol + S-adenosyl-L-methionine
?
show the reaction diagram
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25% of activity with 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
-
-
?
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3,6,7,3'-Tetramethylquercetagetin
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noncompetitive product inhibition
N-ethylmaleimide
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p-chloromercuribenzoate
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S-adenosyl-L-homocysteine
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competitive product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.018
3,7,3'-Trimethylquercetagetin
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-
0.051
S-adenosyl-L-methionine
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
57000
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gel filtration
485587
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 0.2 M potassium phosphate, pH 8, 20% glycerol, 5 mM dithiothreitol, for several months, no loss of activity
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4°C, 4-6 weeks, 80% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Sephacryl S-200, hydroxylapatite, chromatofocusing
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chromatofocusing on Superose 12 and Mono P
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