Information on EC 2.1.1.80 - protein-glutamate O-methyltransferase

New: Word Map on EC 2.1.1.80
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.1.1.80
-
RECOMMENDED NAME
GeneOntology No.
protein-glutamate O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-glutamate O-methyltransferase
Forms ester groups with L-glutamate residues in a number of membrane proteins.
CAS REGISTRY NUMBER
COMMENTARY hide
9055-09-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains S9 and WFS101
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Nostoc punctiforme ATCC 29133
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
the enzyme catalyzes the methylation of the cytosolic domain of the membrane-bound chemotaxis receptors, and plays a pivotal role in the chemotactic signal transduction pathway
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + chemoreceptor carboxyl-terminal pentapeptide NWETF
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + methyl-accepting chemotaxis protein B L-glutamate
S-adenosyl-L-homocysteine + methyl-accepting chemotaxis protein B L-glutamate methyl ester
show the reaction diagram
exklusive substrate of CheR2
-
-
?
S-adenosyl-L-methionine + methyl-accepting chemotaxis protein FrzCD-L-glutamate
S-adenosyl-L-homocysteine + methyl-accepting chemotaxis protein FrzCD-L-glutamate methyl ester
show the reaction diagram
-
full-length FrzF methylates FrzCD on a single residue, E182, while FrzF lacking tetra trico-peptide repeats methylates FrzCD on three residues, E168, E175, and E182
-
-
?
S-adenosyl-L-methionine + PIP2;1 peptide
?
show the reaction diagram
-
the enzyme acts on the N-terminal tail of aquaporin PIP2;1 at Glu6 and is able to methylate both the un- and dimethylated peptide forms
-
-
?
S-adenosyl-L-methionine + protein L-glutamate
S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + [chemotaxis receptor Tar]-L-glutamate
S-adenosyl-L-homocysteine + [chemotaxis receptor Tar]-L-glutamate methyl ester
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + methyl-accepting chemotaxis protein B L-glutamate
S-adenosyl-L-homocysteine + methyl-accepting chemotaxis protein B L-glutamate methyl ester
show the reaction diagram
Q9I6V7
exklusive substrate of CheR2
-
-
?
S-adenosyl-L-methionine + protein L-glutamate
S-adenosyl-L-homocysteine + protein L-glutamate methyl ester
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
A9145C
p-chloromercuribenzoate
-
0.4 mM, 40% inhibition, can be reversed by 12 mM 2-mercaptoethanol
S-adenosyl-L-homocysteine
sinefungin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0077
follicle-stimulating hormone
-
-
-
0.046
gamma-Globulin
-
-
-
0.1
histone
-
-
0.34
Pancreatic ribonuclease
-
-
-
0.00087 - 0.017
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.167
protein L-glutamate
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004
A1945C
-
-
-
0.000065
Ca2+
-
-
0.0002 - 0.0009
S-adenosyl-L-homocysteine
0.0026
sinefungin
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000036
-
methylation of histone II-A in purine starved cells
0.000076
-
-
0.0043
-
thymus enzyme
0.027
-
erythrocyte enzyme
0.03
-
brain enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
approx. 30% of maximal activity at pH 6.0, approx. 65% of maximal activity at pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the enzyme is mostly expressed in the root endodermis and cortex
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
gel filtration
30000
-
gel filtration, SDS-PAGE
32900
-
deduced from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
phosphorylation at Thr3 in the N-terminal domain
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44
isoform CheR1 has a melting temperature of 44C
48
isoform CheR2 has a melting temperature of 48C
49
isoform CheR3 has a melting temperature of 49C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 20-50% glycerol, 5 mM sodium borate, 5 mM EDTA, 2.4 mM 2-mercaptoethanol, pH 9.3, 1 year, no loss of activity
-
0C, 0.33 mM dithiothreitol, 0.33 mg/ml bovine serum albumin, 1 week, no loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Phenyl-Sepharose, Bio-gel P-60, hydroxylapatite
-
ammonium sulfate, Sephadex G-75, SAH-Sepharose, Sephadex G-75
-
brain enzyme, pH 5.1 treatment, ammonium sulfate, SAH-Sepharose, Sephadex G-100
-
DEAE-Biogel, CM-Biogel, ammonium sulfate, S-adenosylhomocystein affinity column
-
HisTrap column chromatography; HisTrap column chromatography; HisTrap column chromatography, and gel filtration
HisTrap HP nickel column chromatography
-
maltose binding protein-tagged enzyme is purified by Ni-NTA column chromatography
-
Ni-NTA column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli C41(DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells
-
expressed in Escherichia coli strain Tuner
-
expression in Escherichia coli at 30C under the control of The T7 polymerase
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA1-19
-
activity is slightly reduced
K46A
-
moderately reduced activity
R47A
-
mutant protein does not express well, all of the CheR protein is found in inclusion bodies
R53A
-
methyltransferase activity is 4% of the wild-type activity
R56A
-
moderately reduced activity
R57A
-
mutant protein does not express well, all of the CheR protein is found in inclusion bodies
R59A
-
moderately reduced activity
Show AA Sequence (4925 entries)
Please use the Sequence Search for a certain query.