Information on EC 2.1.1.77 - protein-L-isoaspartate(D-aspartate) O-methyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria

EC NUMBER
COMMENTARY hide
2.1.1.77
-
RECOMMENDED NAME
GeneOntology No.
protein-L-isoaspartate(D-aspartate) O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
D-aspartate (but not L-aspartate) residues in proteins can also act as acceptors
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-isoaspartate O-methyltransferase
D-Aspartate (but not L-aspartate) residues in proteins can also act as acceptors. Previously also listed as EC 2.1.1.24.
CAS REGISTRY NUMBER
COMMENTARY hide
105638-50-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mushroom
-
-
Manually annotated by BRENDA team
Heynh.
Uniprot
Manually annotated by BRENDA team
pill woodlouse
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cultivar Poinsett 76
-
-
Manually annotated by BRENDA team
carrot, var. Danvers Half Long
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
horse
-
-
Manually annotated by BRENDA team
great slug
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
-
-
-
Manually annotated by BRENDA team
no activity in Aquifex aeolicus
-
-
-
Manually annotated by BRENDA team
no activity in Bacillus subtilis
-
-
-
Manually annotated by BRENDA team
no activity in Borrelia burgdorferi
-
-
-
Manually annotated by BRENDA team
no activity in Haemophilus influenzae
-
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma genitalium
-
-
-
Manually annotated by BRENDA team
no activity in Mycoplasma pneumoniae
-
-
-
Manually annotated by BRENDA team
no activity in Sinorhizobium meliloti
-
-
-
Manually annotated by BRENDA team
no activity in Treponema pallidum
-
-
-
Manually annotated by BRENDA team
strain M-101
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
tomato, Mill.
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 60 S ribosomal protein L27
S-adenosyl-L-homocysteine +
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate
S-adenosyl-L-homocysteine + 7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide-L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 7-nitro-2,1,3-benzoxadiazole-labeled delta sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + 7-nitro-2,1,3-benzoxadiazole-labeled delta sleep-inducing peptide L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + acid trehalase D-aspartate
S-adenosyl-L-homocysteine + acid trehalase D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + actin L-isoaspartate
S-adenosyl-L-homocysteine + actin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ATHSP17.4 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Bcl-xL L-isoaspartate
S-adenosyl-L-homocysteine + Bcl-xL L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + beta-101-lysozyme
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + bovine serum albumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + bovine serum albumin L-isoaspartate
S-adenosyl-L-homocysteine + bovine serum albumin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
enzyme activity is lowest against bovine serum albumin but it doubles when the bovine serum albumin is aged upon incubation in in vitro aging buffer
-
-
?
S-adenosyl-L-methionine + calmodulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + carboxyesterase 12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + cinnamoyl-CoA reductase family protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta sleep inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + delta sleep inducing peptide L-isoaspartate alpha-methyl ester
show the reaction diagram
-
highest activity
-
-
?
S-adenosyl-L-methionine + delta sleep-inducing peptide
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta-sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + delta-sleep-inducing peptide L-aspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + delta-sleep-inducing peptide L-isoaspartate
S-adenosyl-L-homocysteine + delta-sleep-inducing peptide L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + early flowering 8 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + gamma-globulin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + gamma-globulin L-isoaspartate
S-adenosyl-L-homocysteine + gamma-globulin L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + gelatin I
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + GFDL-isoD-GGGVG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + histidine phosphocarrier protein (isoAsp-38)
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histidine phosphocarrier protein-2 (isoAsp-12,-38)
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histone H2A L-isoaspartate
S-adenosyl-L-homocysteine + histone H2A L-isoaspartate alpha-methyl ester
show the reaction diagram
-
in chicken, iso-L-Asp occurs at the Asp121-Ser122 bond in the flexible C-terminal tail of histone H2A
-
-
?
S-adenosyl-L-methionine + histone H2B L-isoaspartate
S-adenosyl-L-homocysteine + histone H2B L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + histone H4 D-aspartate
S-adenosyl-L-homocysteine + histone H4 D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Hsp70 L-isoaspartate
S-adenosyl-L-homocysteine + Hsp70 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Hsp90 L-isoaspartate
S-adenosyl-L-homocysteine + Hsp90 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + isoACTH
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
r
S-adenosyl-L-methionine + isoAspDSIP
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA (D-Asp) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + KASA (isoD) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + KASA(iso-D)LAKY
S-adenosyl-L-homocysteine + KASA(isoaspartate alpha-methyl ester)LAKY
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + KASA(iso-D)LAKY
S-adenosyl-L-homocysteine + KASA(L-isoaspartate alpha-methyl ester)LAKY
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + KASA-iso-L-Asp-LAKY
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + KASA-L-isoaspartyl-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + KMK-isoD-TDSEEEIR
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KQVV-isoD-SAYEVIK
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala
S-adenosyl-L-homocysteine + L-Val-L-Tyr-L-Pro-(L-iso-Asp alpha methyl ester)-L-His-L-Ala
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + LSH-isoD-GY-Cya-L
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + LSH-isoD-GYCL
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + mitochondrial glycoprotein family protein/MAM33
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPF-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPL-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAV-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAVA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + native lysozyme
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + oligocellula 2 protein
S-adenosyl-L-homocysteine +
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + ovalbumin L-isoaspartate
S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + PIRL8 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + plant RNA helicase 75 L-isoaspartate
S-adenosyl-L-homocysteine + plant RNA helicase 75 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + PRH75 protein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-aspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein Lisoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein p53
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
the enzyme mediates methylation of protein p53 at isoaspartate residues 29 and 30
-
-
?
S-adenosyl-L-methionine + succinamide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + synapsin I
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + synuclein L-isoaspartate
S-adenosyl-L-homocysteine + synuclein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + T1-isoAsp-12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + T4-isoAsp-38
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + UDP-xylose synthase 5
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + V2-isoAsp-12
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + V4-isoAsp-38
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + Val-Tyr-Pro(L-iso-aspartate)-His-Ala
S-adenosyl-L-homocysteine + Val-Tyr-Pro(L-isoaspartate alpha-methyl ester)-His-Ala
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + VYP (isoD) HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + VYP(iso-D)HA
S-adenosyl-L-homocysteine + VYP(isoaspartate alpha-methyl ester)HA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + VYP(iso-D)HA
S-adenosyl-L-homocysteine + VYP(L-isoaspartate alpha-methyl ester)HA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + VYP-D-alpha-Asp-GA
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-CA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-Cya-G
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-DA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-isoD-GA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + VYP-L-beta-Asp-GA
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-CA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-GA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-GHG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + VYP-L-isoAsp-RR
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoaspartyl-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoAsp-GHG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-G-Cya-G
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-GCG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + YVS-isoD-GDG
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + acid trehalase D-aspartate
S-adenosyl-L-homocysteine + acid trehalase D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + histone H4 D-aspartate
S-adenosyl-L-homocysteine + histone H4 D-aspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + plant RNA helicase 75 L-isoaspartate
S-adenosyl-L-homocysteine + plant RNA helicase 75 L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein D-isoaspartate
S-adenosyl-L-homocysteine + protein D-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein Lisoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
-
tightly bound cofactor
S-adenosyl-L-homocysteine
S-adenosyl-L-methionine
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-deoxy-5'-methylthioadenosine
adenosine
-
-
S-adenosyl-L-homocysteine
S-adenosylhomocysteine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CGP3466B
-
-
-
Urea
-
recombinant enzyme relative activity is greatest in liquid assays in 1 M urea
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.055
7-nitro-2,1,3-benzoxadiazole-delta sleep-inducing peptide -L-isoaspartate
0.183
beta-101-lysozyme
-
-
-
0.03336
delta sleep inducing peptide L-isoaspartate
-
at pH 7.0 and 30C
-
0.0035
GFDL-isoD-GGGVG
-
-
0.00358
histidine phosphocarrier protein-1 (isoAsp-38)
-
-
-
0.197
HPr-2 (isoAsp-12,-38)
-
-
-
0.013
isoACTH
-
-
0.0164
isoAspDSIP
-
-
0.023 - 2.7
KASA (D-Asp) LAKY
0.00026 - 0.0506
KASA (isoAsp) LAKY
0.0004 - 0.0506
KASA (isoD) LAKY
0.0145 - 0.0286
KASA(iso-D)LAKY
0.08 - 0.092
KASA-L-isoAsp-LAKY
0.00055
KQVV-isoD-SAYEVIK
-
-
0.00617
KVTCK-isoD-GQTNCYQSK
-
-
0.25
L-Val-L-Tyr-L-Pro-(L-iso-Asp)-L-His-L-Ala
in HEPES buffer, pH 7.5, at 37C
0.017
LSH-isoD-GY-Cya-L
-
-
0.0036
LSH-isoD-GYCL
-
-
0.375 - 11
N-succinyl-AAVAp-nitroanilide
1.081
native lysozyme
-
-
-
0.00463 - 12.7
ovalbumin
0.0423 - 0.159
ovalbumin L-isoaspartate
0.00051
plant RNA helicase 75 L-isoaspartate
-
at pH 7.5 and 25C
-
0.00089 - 0.03
S-adenosyl-L-methionine
0.0012
synapsin I
-
-
-
0.0229
T1-isoAsp-12
-
-
-
0.339
T4-isoAsp-38
-
-
-
0.0274
V2-isoAsp-12
-
-
-
0.0975
V4-isoAsp-38
-
-
-
0.208 - 0.27
Val-Tyr-Pro(L-iso-aspartate)-His-Ala
0.00029 - 0.0118
VYP (isoAsp) HA
0.0041 - 0.0336
VYP (isoD) HA
0.00167
VYP-isoD-AA
-
-
-
0.0028
VYP-isoD-CA
-
-
-
0.175
VYP-isoD-Cya-G
-
-
-
0.00295
VYP-isoD-DA
-
-
-
0.00425 - 0.0062
VYP-isoD-GA
0.00304
VYP-isoD-SA
-
-
-
0.0068
VYP-L-isoAsp-GA
-
-
-
0.00029 - 0.38
VYP-L-isoAsp-HA
0.38
VYP-L-isoaspartyl-HA
-
in sodium citrate buffer at pH 6.0 and 45C
0.005
WM-isoD-F-NH2
-
-
-
0.0155
YVS-isoAsp-GHG
-
-
-
0.104
YVS-isoD-G-Cya-G
-
-
-
0.0046
YVS-isoD-GCG
-
-
-
0.469
YVS-isoD-GDG
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
233.3
delta sleep inducing peptide L-isoaspartate
Cyberlindnera jadinii
-
at pH 7.0 and 30C
-
203.9
S-adenosyl-L-methionine
Cyberlindnera jadinii
-
at pH 7.0 and 30C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
S-adenosyl-L-homocysteine
-
at pH 7.0 and 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000057
-
-
0.0000069
-
-
0.0000127
-
VYP-L-isoAsp-GHG as substrate
0.0000175
-
-
0.0000215
-
-
0.00013
-
gamma-globulin as substrate
0.000229
-
VYP-L-isoAsp-RR as substrate
0.000233
-
-
0.000433
-
ovalbumin as substrate
0.001441
-
VYP-L-isoAsp-CA as substrate
0.004179
-
KASA-L-isoAsp-LAKY as substrate
0.00427
-
-
0.005028
-
VYP-L-isoAsp-HA as substrate
0.011
-
using the delta sleep-inducing peptide as the methyl acceptor
0.012 - 0.016
-
-
0.016 - 0.02
-
gamma-globulin as methyl acceptor
0.021
-
recombinant enzyme, at 37C
0.4014
-
crude enzyme, at pH 7.0 and 30C
17.01
-
after 43.41fold purification, at pH 7.0 and 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
maximum enzyme activity at pH 6.0 to 8.0, while the enzyme activity is significantly inhibited above pH 9.5; maximum enzyme activity at pH 6.0 to 8.0, while the enzyme activity is significantly inhibited above pH 9.5
7 - 8
-
recombinant enzyme, expressed in Escherichia coli
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 55
-
-
45 - 55
55
-
recombinant enzyme, expressed in E. coli
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 65
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
isoform PIMT1, isoelectric focusing
6.7
-
isoform PIMT2 TISIIIalphaomega, isoelectric focusing; isoform PIMT2 TISIIIalphapsi, isoelectric focusing
7.9
-
isoform PIMT2 TISIIIbetapsi, isoelectric focusing
9
-
isoform PIMT2 TISIIalphaomega, isoelectric focusing; isoform PIMT2 TISIIalphapsi, isoelectric focusing
9.1
-
isoform PIMT2 TISIIbetapsi, isoelectric focusing
9.4
-
isoform PIMT2 TISIalphaomega, isoelectric focusing; isoform PIMT2 TISIalphapsi, isoelectric focusing
9.5
-
isoform PIMT2 TISIbetapsi, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
69% reduced PIMT expression as compared to normal brains
Manually annotated by BRENDA team
-
PIMT expression in bovine aortic endothelial cells is regulated by cell detachment and readhesion to a substratum
Manually annotated by BRENDA team
-
enzyme expression and enzyme activity are significantly decreased in all grades of tumors, 76% reduced PIMT expression in pilocytic astrocytomas (grade I), 46% reduced PIMT expression in astrocytomas (grade II), 69% reduced PIMT expression in anaplastic astrocytomas (grade III); enzyme expression and enzyme activity are significantly decreased in all grades of tumors, 76% reduced PIMT expression in pilocytic astrocytomas (grade I), 46% reduced PIMT expression in astrocytomas (grade II), 69% reduced PIMT expression in anaplastic astrocytomas (grade III), 80% reduced PIMT expression in glioblastomas (grade IV) as compared to normal brains
Manually annotated by BRENDA team
-
treatment of astrocytoma cells (U-87) with direct pharmacological GSK-3 inhibitors stimulate PIMT expression (2fold). Results demonstrate regulation of PIMT expression by GSK-3 inhibitor lithium at both the transcriptional and the translational levels. Inhibition by siRNA of GSK-3 and beta-catenin modulates expression of the PIMT in accordance with GSK-3 pharmacological inhibition. Valproic acid up-regulates phospho-GSK-3b (Ser9), beta-catenin and PIMT levels similarly to lithium
Manually annotated by BRENDA team
-
PIMT levels are regulated by cell adhesion in various cancer cell lines. The upregulation of PIMT expression could be prevented by an anti-alphavbeta3 antibody (LM609) or by a cyclic RGD peptide (XJ735) specific to integrin alphavbeta3. PIMT expression returns to the basal level when cells are replated on a substratum after detachment, though downregulation of PIMT expression could be partly prevented by the PI3K inhibitors LY294002 and wortmannin, as well as by the proteasome inhibitors MG-132, lactacystin, and beta-lactone
Manually annotated by BRENDA team
-
80% reduced PIMT expression as compared to normal brains
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
; head region, lateral interneurons and tail region
Manually annotated by BRENDA team
-
76% reduced PIMT expression as compared to normal brain
Manually annotated by BRENDA team
-
highest expression of isoform PIMT1
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team